Earthquake‐induced landslide scenarios for seismic microzonation. Application to the Accumoli area (Rieti, Italy)

Scenarios of earthquake-induced landslides are necessary for seismic microzonation (SM) studies since they must be integrated with the mapping of instability areas. The PARSIFAL (Probabilistic Approach to pRovide Scenarios of earthquake‐Induced slope FAiLures) approach provides extensive analyses, over tens to thousands of square kilometers, and is designed as a fully comprehensive methodology to output expected scenarios which depend on seismic input and saturation conditions. This allows to attribute a rating, in terms of severity level, to the landslide-prone slope areas in view of future engineering studies and designs. PARSIFAL takes into account first-time rock- and earth-slides as well as re-activations of existing landslides performing slope stability analyses of different failure mechanisms. The results consist of mapping earthquake-induced landslide scenarios in terms of exceedance probability of critical threshold values of co-seismic displacements (P[D≥Dc|a(t),ay]). PARSIFAL was applied in the framework of level 3 SM studies over the municipality area of Accumoli (Rieti, Italy), strongly struck by the 2016 seismic sequence of Central Apennines. The use of the PARSIFAL was tested for the first time to screen the Susceptibility Zones (ZSFR) from the Attention Zones (ZAFR) in the category of the unstable areas, according to the guidelines by Italian Civil Protection. The results obtained were in a GIS-based mapping representing the possibility for a landslide to be induced by an earthquake (with a return period of 475 years) in three different saturation scenarios (i.e. dry, average, full). Only 41% of the landslide-prone areas in the Municipality of Accumoli are existing events, while the remaining 59% is characterized by first-time earth- or rock-slides. In dry conditions, unstable conditions or P[D≥Dc|a(t),ay]>0 were for 54% of existing landslides, 17% of first-time rock-slides and 1% of first-time earth- slides. In full saturation conditions, the findings are much more severe since unstable conditions or P[D≥Dc|a(t),ay]>0 were found for 58% of the existing landslides and for more than 80% of first-time rock- and earth-slides. Moreover, comparison of the total area of the ZAFR versus ZSFR, resulted in PARSIFAL screening reducing of 22% of the mapped ZAFR

Innovative Energy Approach for Design and Sizing of Electric Vehicle Charging Infrastructure

In Italy, the availability of service areas (SAs) equipped with charging stations (CSs) for electric vehicles (EVs) on highways is limited in comparison to the total number of service areas. The scope of this work is to create a prototype and show a different approach to assessing the number of inlets required on highways. The proposed method estimates the energy requirements for the future electric fleet on highways. It is based on an energy conversion that starts with the fuel sold in the highway network and ends with the number of charging inlets. A proposed benchmark method estimates energy requirements for the electric fleet using consolidated values and statistics about refueling attitudes, with factors for range correction and winter conditions. The results depend on assumptions about future car distribution, with varying numbers of required inlets. The analysis revealed that vehicle traffic is a critical factor in determining the number of required charging inlets, with significant variance between different SAs. This study highlights the necessity of incorporating factors like weather, car charging power, and the future EV range into these estimations. The findings are useful for planning EV charging infrastructure, especially along major traffic routes and in urban areas with high-range vehicles relying on High-Power DC (HPDC) charging. The model’s applicability to urban scenarios can be improved by considering the proportion of energy recharged at the destination. A key limitation is the lack of detailed origin–destination (OD) highway data, leading to some uncertainty in the calculated range ratio coefficient and underscoring the need for future research to refine this model

γδ T Cells Cross-Link Innate and Adaptive Immunity in Mycobacterium tuberculosis Infection

Protective immunity against mycobacterial infections such as Mycobacterium tuberculosis is mediated by interactions between specific T cells and activated antigen presenting cells. To date, many aspects of mycobacterial immunity have shown that innate cells could be the key elements that substantially may influence the subsequent adaptive host response. During the early phases of infection, innate lymphocyte subsets play a pivotal role in this context. Here we summarize the findings of recent investigations on γδ T lymphocytes and their role in tuberculosis immunity

Histoire de la Chine

Port. con grab. xil.Viñetas xil.Sign.: []1, A-T12, V3

A murine systemic model for shigellosis

Summary Shigella spp. are pathogenic bacteria responsible for bacillary dysentery in humans. The major lesions in colonic mucosa are intense inflammation with apoptosis of macrophages and release of pro-inflammatory cytokines. The study of shigellosis is hindered by the natural resistance of rodents to oral infection with Shigella. Therefore, animal models exploit other routes of infection. Here, we describe a novel murine model in which animals receive shigellae via the caudal vein. Mice infected with 5 × 106 (LD50) virulent shigellae died at 48 h post infection, whereas animals receiving non-invasive mutants survived. The liver is the main target of infection, where shigellae induce microgranuloma formation. In mice infected with invasive bacteria, high frequency of apoptotic cells is observed within hepatic microgranulomas along with significant levels of mRNA for pro-inflammatory cytokines such as IL-1β, IL-18, IL-12 and IFN-γ. Moreover, in the blood of these animals high levels of IL-6 and transaminases are detected. Our results demonstrate the intravenous model is suitable for pathogenicity studies and useful to explore the immune response after Shigella infection

Probabilistic Approach to Provide Scenarios of Earthquake-Induced Slope Failures (PARSIFAL) Applied to the Alcoy Basin (South Spain)

The PARSIFAL (Probabilistic Approach to pRovide Scenarios of earthquake-Induced slope FAiLures) approach was applied in the basin of Alcoy (Alicante, South Spain), to provide a comprehensive scenario of earthquake-induced landslides. The basin of Alcoy is well known for several historical landslides, mainly represented by earth-slides, that involve urban settlement as well as infrastructures (i.e., roads, bridges). The PARSIFAL overcomes several limits existing in other approaches, allowing the concomitant analyses of: (i) first-time landslides (due to both rock-slope failures and shallow earth-slides) and reactivations of existing landslides; (ii) slope stability analyses of different failure mechanisms; (iii) comprehensive mapping of earthquake-induced landslide scenarios in terms of exceedance probability of critical threshold values of co-seismic displacements. Geotechnical data were used to constrain the slope stability analysis, while specific field surveys were carried out to measure jointing and strength conditions of rock masses and to inventory already existing landslides. GIS-based susceptibility analyses were performed to assess the proneness to shallow earth-slides as well as to verify kinematic compatibility to planar or wedge rock-slides and to topples. The experienced application of PARSIFAL to the Alcoy basin: (i) confirms the suitability of the approach at a municipality scale, (ii) outputs the main role of saturation in conditioning slope instabilities in this case study, (iii) demonstrates the reliability of the obtained results respect to the historical data.This work was partially funded by Spanish MINECO and European funds under project EPILATES (CGL2015.65602-R) and by the University of Alicante, research group VIGROB-184

Design of a study to investigate the mechanisms of obstructive sleep apnoea by means of drug-induced sleep endoscopy

Background Obstructive sleep apnoea (OSA) is an independent risk factor of hypertension and cardiovascular diseases. Recurrent episodes of upper airways collapse during sleep causing blood oxygen desaturation, hypercapnia, and micro-arousals, are known to activate the sympathetic nervous system (SNS). However, whether changes in the renin-angiotensin-aldosterone system and endothelial activation also occur remains contentious. Methods Based on routine use of drug-induced sleep endoscopy (DISE) for the work-up of OSA patients in our centre, we designed a prospective study to investigate the haemodynamic and humoral changes occurring during the apnoeic episodes reproduced in vivo in the course of DISE. Specifically, plasma aldosterone concentration and renin activity, C-terminal fragment of proendothelin-1, as a marker of endothelial damage, and free plasma catecholamines, will be measured at fixed times during DISE. The activity of catechol-O-methyltransferase (COMT), a key catecholamine-inactivating enzyme that has been scantly investigated thus far owing to the lack of commercially available kits, will be also determined by a newly developed high performance liquid chromatography method, which is herein described. Results and conclusions The aim of this study is to provide novel information on the haemodynamic, hormonal, and SNS changes, and also on COMT activity modification concomitantly occurring during apnoea, thus contributing substantively to the understanding of the pathophysiology of OSA

YAP regulates cell mechanics by controlling focal adhesion assembly

Hippo effectors YAP/TAZ act as on-off mechanosensing switches by sensing modifications in extracellular matrix (ECM) composition and mechanics. The regulation of their activity has been described by a hierarchical model in which elements of Hippo pathway are under the control of focal adhesions (FAs). Here we unveil the molecular mechanism by which cell spreading and RhoA GTPase activity control FA formation through YAP to stabilize the anchorage of the actin cytoskeleton to the cell membrane. This mechanism requires YAP co-transcriptional function and involves the activation of genes encoding for integrins and FA docking proteins. Tuning YAP transcriptional activity leads to the modification of cell mechanics, force development and adhesion strength, and determines cell shape, migration and differentiation. These results provide new insights into the mechanism of YAP mechanosensing activity and qualify this Hippo effector as the key determinant of cell mechanics in response to ECM cues.Peer reviewe

Influence of geological complexities on local seismic response in the municipality of Forio (Ischia Island, Italy)

Seismic response studies carried out in the Municipality of Forio on Ischia (NA), southern Italy, following the 21st August 2017 earthquake allowed to detect local effects related to specific geological-structural settings that reflect the complexity of the volcanic context which characterises the entire island of Ischia and, more specifically, the western sector of Mt. Epomeo and the adjacent coastal plain of Forio. In particular, the following features have been observed: i) polarization and amplification effects in the proximity of tectonic elements that dissect the Zaro promontory, where volcanic deposits from massive to stratified widely outcrop; ii) stratigraphic resonances on significantly variable frequency values, changing within distances of a few hundred meters, which can be related to the juxtaposition of landslide debris (such as debris-/rockavalanches and lahar) in correspondence with the town of Forio; iii) seismic amplification in the sector involved in the ongoing gravitational deformation of Mt. Nuovo even in the absence of polarization of the particle motion. The peculiarities of the geological contexts analysed in the Forio Municipality allow to apply different interpretative schemes that vary from stratigraphic resonance (one-dimensional model of resonant column) mostly controlled by thickness and wave velocity in soft soils onto a stiff bedrock, to the resonance of jointed rock masses which are not completely released from the adjacent bedrock so avoiding typical free vibrations with normal modes (three-dimensional oscillating mass model), or to the interaction in the near surface with physical discontinuities responsible for modifying the physical properties of surface waves polarizing and amplifying them. The collected evidence of local seismic response in Forio exemplifies how not conventional interpretative keys for seismic zoning can be proposed to identify sectors whose response schemes do not necessarily fall within the standard of stratigraphic or topographical amplification adopted by current national guidelines for Seismic Microzonation (SM) studies. In particular, the evidence of local seismic response collected for the Forio Municipality were taken into account in the SM and relative products that were realised in the following year