Adaptação cultural e validação da versão portuguesa do questionário Medical Outcomes Study Social Support Survey (MOS-SSS)

O objectivo deste trabalho é calcular as propriedades psicométricas da versão portuguesa do questionário Medical Outcomes Study Social Support Survey (MOS-SSS). Este questionário foi traduzido e adaptado à cultura portuguesa. A amostra é constituída por 101 doentes com doença crónica, utentes de um centro de saúde rural de Portugal. A idade média é 63,4 anos; 56,4% são mulheres. 29% são analfabetos e 2% possuem estudos superiores. 78% sofrem de hipertensão arterial e 56,4% sofrem de diabetes mellitus tipo 2. A consistência interna foi avaliada com o alpha de Cronbach. Depois, foi realizada uma análise factorial exploratória e confirmatória com o objectivo de confirmar a validade e fiabilidade da escala e as suas características multidimensionais. A confiabilidade teste-reteste realizada às duas semanas foi estimada usando a análise kappa ponderado para as variáveis ordinais e o coeficiente de correlação intraclasse para as variáveis quantitativas. A alpha de Cronbach para as subescalas varia entre 0,873 e 0,967 no teste e 0,862 e 0,972 no reteste. A análise factorial exploratória revelou a existência de quatro factores (apoio emocional, material, afectivo e interacção social positiva) que explicam o 72,71% da variância. A análise factorial confirmatória permite concluir a existência destes quatro factores que permitem a aplicação da escala com os itens do questionário original. Os índices de ajustamento são satisfatórios e verificam a estrutura inicial, apresentando valores de χ2/df =2,01; GFI =0,998; CFI=0,999; AGFI =0,998; TLI =0,999; NFI =0,998; SRMR =0,332; RMSEA =0,76. A confiabilidade teste-reteste às duas semanas da versão portuguesa do MOS-SSS foi estimada pelo coeficiente de correlação intraclasse que variou entre 0,941 e 0,966 para as quatro dimensões do questionário e para o índice de apoio total. Os índices kappa ponderados variaram entre 0,67 e 0,87 para todos os itens. A versão portuguesa do MOS-SSS demonstrou boas características psicométricas e parece ser útil para avaliar as características multidimensionais do apoio social em população portuguesa.The aim of this study was the assesment of psychometric properties of the Portuguese version of the instrument «Medical Outcomes Study – Social Support Survey (MOSSSS) ». This questionnaire has been translated and adapted in a Portuguese sample of 101 patients with chronic illness of a rural health centre in Portugal. The average age of patients was 63.4 years, 56.4% female. 29% were illiterate and 2% had completed high school. 78% had arterial hypertension and the 56.4% had diabetes mellitus type 2. The internal consistency was evaluated using Cronbach’s alpha. Exploratory and Confirmatory factor analysis were performed in order to confirm reliability and validity of the scale and its multidimensional characteristics. The 2-week test-retest reliability was estimated using weighted kappa for the ordinals variables and intraclass coefficient correlation for the quantitative variables. Cronbach’s alphas for the subscales ranged from 0.873 to 0.967 at test, and 0.862 to 0.972 at retest. Exploratory factor analysis revealed the existence of four factors (emotional, tangible, positive interaction and affection support) that explain the 72.71% of the variance. Confirmatory factor analysis supported the existence of four factors that allowed the application of the scale with original items. The goodness-of-fit measures corroborate the initial structure, with χ2/ df=2.01, GFI=0.998, CFI=0.999, AGFI=0.998, TLI=0.999, NFI=0.998, SRMR=0.332, RMSEA=0.76. The 2-weeks test-retest reliability of the Portuguese MOS-SSS as measured by the intraclass correlation coefficient was ranged from 0.941 to 0.966 for the four dimensions and the overall support index. The weighted kappa was ranged from 0.67 to 0.87 for all the items. The MOS-SSS Portuguese version demonstrates good psychometric properties and seems to be useful to measure multidimensional aspects of social support in the Portuguese population.Sociedade Espanhola de Medicina Familiar e Comunitária (SEMFYC

Do the low molecular weight heparins improve efficacy and safety of the treatment of deep venous thrombosis? A meta-analysis

We compared the efficacy and safety of low molecular weight heparins (LMWH) and unfractionated heparin (UFH) in the treatement of deep venous thrombosis (DVT). A comparison between two daily subcutaneous injections of LMWH against a single injection was also performed. DESIGN AND METHODS: The study was performed by a meta-analysis. Clot improvement in venography, recurrency, total mortality and major hemorrhages were assessed in 4,472 patients with DVT from 21 studies treated with subcutaneous LMWH or UFH. RESULTS: An improvement in clot reduction (odds ratio 0.73, 95% confidence interval 0.59 to 0.90, p = 0.004), a decrease in total mortality (0. 68, 0.50 to 0.91, p = 0.012) and a lower incidence of hemorrhage (0. 65, 0.43 to 0.98, p = 0.047) were observed in LMWH treated patients. There were no differences in recurrences (0.78, 0.59 to 1.04, p = 0. 10). A single dose of LMWH was better than two in reducing major bleeding (c2 = 4.99, p = 0.025); however, the two dose regimen was more effective in clot reduction (c2 = 8.56, p = 0.004). INTERPRETATION AND CONCLUSIONS: LMWH is superior to UFH in terms of safety and efficacy. A single daily dose of LMWH dose is a suitable therapeutic regimen and could facilitate the outpatient treatment of venous thromboembolism

Synthesis of a Se0/Calcite Composite Using Hydrothermal Carbonation of Ca(OH)2 Coupled to a Complex Selenocystine Fragmentation

International audienceElemental selenium (Se0)/calcite composites were synthesized in a batch system by hydrothermal carbonation of calcium hydroxide under high CO2−Ar pressure (90 bar) and high temperature (90 °C) coupled to a complex selenocystine fragmentation. Under O2-poor conditions, the composite consisted predominantly of spherical, amorphous nanoparticles of elemental red selenium (<500 nm) deposited on the calcite matrix. Conversely, under O2-rich conditions, the composite consisted rod-shaped, well-crystallized microparticles of elemental gray selenium (<25 µm) dispersed in the calcite matrix. The carbonate matrix was constituted by nano- to microrhombohedral crystals (<2 µm) and micrometric agglomerates and/or aggregates (<5 µm). Our results present a new synthesis path to Se0/calcite composites, with spherical or rod-shaped Se0 morphology with high potential for medical (e.g., dietary supplement) or industrial (e.g., pigments) applications. Furthermore, this study may have implications in the field of biomineralization

Changes in plasma fatty acid composition are associated with improvements in obesity and related metabolic disorders: A therapeutic approach to overweight adolescents

Background & aims: In recent years, obesity has reached alarming levels among children and adolescents. The study of plasma fatty acid (FA) composition, as a reflection of diet, and its associations with other parameters, that are closely linked to obesity and the cardiometabolic profile, may be useful for setting nutritional goals for obesity treatment and prevention. This study explored the relationship between plasma FA levels and body fat and cardiometabolic risk markers, in overweight adolescents. Methods: A multidisciplinary weight loss program was followed by 127 overweight and obese adolescents aged 12-17 years old. Plasma FA composition, anthropometric indicators of adiposity and biochemical parameters were analyzed at baseline, two months (the end of the intensive intervention phase) and six months (the end of the extensive phase). Results: While saturated fatty acid (SFA) and n-6 polyunsaturated fatty acid (PUFA) levels decreased significantly during the intervention, monounsaturated fatty acid (MUFA) and n-3 PUFA showed the opposite trend. The decrease in SFA C14:0 was associated with a reduction in total and LDL cholesterol, apolipoprotein B and insulin. The increase in MUFAs, especially C18:1n-9, was related to a reduction in weight, fat mass, fat mass index and glucose. Regarding PUFAs, changes in the n-3 series were not associated with any of the parameters studied, whereas the reduction in n-6 PUFA5 was directly related to weight, fat mass, total and HDL cholesterol, apolipoprotein Al, glucose and insulin, and inversely associated with diastolic blood pressure. The adolescents with greater weight loss presented significant changes in MUFAs, n-6 PUFA5 and C14:0. Conclusions: Modifications in plasma FA composition were associated with adiposity reduction and cardiometabolic profile improvement in an anti-obesity program aimed at adolescents. The changes observed in FA composition were related to the success of the treatment, since the individuals most affected by these variations were those who presented the greatest weight loss

Feminicide and counterdata production: Activist efforts to monitor and challenge gender-related violence

Gender-related violence against women and its lethal outcome, feminicide, are a serious problem throughout the world. Official government data on gender violence and feminicide are often absent, incomplete, infrequently updated, and contested. We draw on data feminism to situate feminicide data as missing data. Building on qualitative interviews, this study discusses the informatic work of ten activist and civil society organizations across six countries who combat missing data by producing counterdata. Activists enact alternative epistemological approaches to data science that center care, memory, and justice. Activists also face significant information challenges that increase monitoring labor and add emotional burden to reading about violent deaths. This work contributes to literature on data activism and critical data studies, proposing feminicide data practices as an important research subject. The empirical insights contribute to human-computer interaction (HCI) research, suggesting ways that the field may support and sustain the counterdata production practices of activists

Development of an In Vitro Model for the Multi-Parametric Quantification of the Cellular Interactions between Candida Yeasts and Phagocytes

We developed a new in vitro model for a multi-parameter characterization of the time course interaction of Candida fungal cells with J774 murine macrophages and human neutrophils, based on the use of combined microscopy, fluorometry, flow cytometry and viability assays. Using fluorochromes specific to phagocytes and yeasts, we could accurately quantify various parameters simultaneously in a single infection experiment: at the individual cell level, we measured the association of phagocytes to fungal cells and phagocyte survival, and monitored in parallel the overall phagocytosis process by measuring the part of ingested fungal cells among the total fungal biomass that changed over time. Candida albicans, C. glabrata, and C. lusitaniae were used as a proof of concept: they exhibited species-specific differences in their association rate with phagocytes. The fungal biomass uptaken by the phagocytes differed significantly according to the Candida species. The measure of the survival of fungal and immune cells during the interaction showed that C. albicans was the more aggressive yeast in vitro, destroying the vast majority of the phagocytes within five hours. All three species of Candida were able to survive and to escape macrophage phagocytosis either by the intraphagocytic yeast-to-hyphae transition (C. albicans) and the fungal cell multiplication until phagocytes burst (C. glabrata, C. lusitaniae), or by the avoidance of phagocytosis (C. lusitaniae). We demonstrated that our model was sensitive enough to quantify small variations of the parameters of the interaction. The method has been conceived to be amenable to the high-throughput screening of mutants in order to unravel the molecular mechanisms involved in the interaction between yeasts and host phagocytes