4NQO Carcinogenesis: A Model of Oral Squamous Cell Carcinoma

Martinez, CAR (reprint author), Univ Talca, Edificio Dept Ciencias Basicas Biomed, Ave Lircay S-N,Oficina 1, Talca, Chile.The experimental oral carcinogenesis induced by the chemical 4-nitroquinoline 1-oxide (4NQO) is one of the most frequent in the study of squamous cell carcinoma of the oral cavity (CCEC). The clear advantage is that the model is very similar to the physiological process of malignancy. The model has clear benefits by and is suitable for applications in therapeutic research

Oral administration of curcumin (curcuma Longa) can attenuate the neutrophil inflammatory response in zymosan-induced arthritis in rats

PURPOSE: To evaluate the effect of curcumin in the acute phase of zymosan-induced arthritis.METHODS: Twenty-eight male rats were subjected to intra-articular infiltration of zymosan of both knees and, in four the infiltration was made with saline. The animals were divided into five groups second received every six hours by gavage: corn oil by (positive and negative control); curcumin (100 mg/kg); prednisone 1 mg/kg/day; prednisone 8 mg/kg. All animals were sacrificed after six, 12, 24 and 48 hours of the infiltration. The knees were removed for evaluation of neutrophil infiltration. The number of neutrophils was counted by computer-assisted analysis of the images. The neutrophil infiltrate was stratified into four grades: 0 = normal; + = mild; ++/+++ = moderate; > ++++ = severe. The results were compared using the Mann-Whitney test and the variance by Kruskal-Wallis test adopting a significance level of 5% (p<0.05).RESULTS: Curcumin reduces inflammatory activity in the first six hours after zymosan-induced arthritis when compared to saline (p<0.01). This was also observed in animals subjected to administration of prednisone (1 mg/kg) and those treated with prednisone (8 mg/kg). Curcumin was more effective than lower doses of prednisone in the first six hours after induction of the arthritis. After 12, 24 and 48 hours, curcumin does not have the same anti-inflammatory effects when compared to prednisone. After 48 hours, prednisone is more effective than curcumin in reducing the inflammatory infiltrate regardless of the dose of prednisone used.CONCLUSION: Oral administration of curcumin reduces inflammation in the first six hours after experimentally zymosan-induced arthritis.To evaluate the effect of curcumin in the acute phase of zymosan-induced arthritis.METHODS: Twenty-eight male rats were subjected to intra-articular infiltration of zymosan of both knees and, in four the infiltration was made with saline. The animals were2911727734sem informaçãosem informaçãoLawrence, R.C., Felson, D.T., Helmick, C.G., Arnold, L.M., Choi, H., Deyo, R.A., Gabriel, S., Wolfe, F., National Arthritis Data Workgroup. Estimates of the prevalence of arthritis and other rheumatic conditions in the United States. Part II (2008) Arthritis Rheum, 58 (1), pp. 26-35. , JanMahajan, A., Verma, S., Tandon, V., Osteoarthritis (2005) J Ass Physicians India, 53, pp. 634-641. , Jul, PMID: 16190135Arden, N., Nevitt, M.C., Osteoarthritis: Epidemiology (2006) Best Pract Res Clin Rheumatol, 20 (1), pp. 3-25. , Feb, PMID:16483904Dibonaventura, M., Gupta, S., McDonald, M., Sadosky, A., Evaluating the health and economic impact of osteoarthritis pain in the workforce: results from the National Health and Wellness Survey (2011) BMC Musculoskelet Disord, 12, p. 83. , Apr 28Rocha, F.A., Andrade, L.E., Jancar, S., Immune complex induced arthritis in rats: Role of lipid mediators on cell infiltration (1996) Med Inflam, 5 (2), pp. 104-109. , PMID: 18475706Henrotin, Y.E., Bruckner, P., Pujol, J.P., The role of reactive oxygen species in homeostasis and degradation of cartilage (2003) Osteoarthritis Cartilage, 11 (10), pp. 747-755. , Oct, PMID:13129694Fermor, B., Christensen, S.E., Youn, I., Cernanec, J.M., Davies, C.M., Weinberg, J.B., Oxygen, nitric oxide and articular cartilage (2007) Eur Cell Mater., 13, pp. 56-65. , Apr 11, discussion 65, PMID: 17427142Jurenka, J.S., Anti-inflammatory properties of curcumin, a major constituent of Curcuma longa: A review of pre-clinical and clinical research (2009) Altern Med Rev, 14 (2), pp. 141-153. , Jun, PMID:19594223Chopra, A., Lavin, P., Patwardhan, B., Chitre, D., A 32-week randomized, placebo-controlled clinical evaluation of RA-11, an Ayurvedic drug, on osteoarthritis of the knees (2004) Clin Rheumatol, 10 (5), pp. 236-245. , Oct, PMID: 17043520Park, C., Moon, D.O., Choi, I.W., Choi, B.T., Nam, T.J., Rhu, C.H., Kwon, T.K., Choi, Y.H., Curcumin induces apoptosis and inhibits prostaglandin E(2) production in synovial fibroblasts of patients with rheumatoid arthritis (2007) Int J Mol Med, 20 (3), pp. 365-372. , Sep, PMID: 17671742Funk, J.L., Oyarzo, J.N., Frye, J.B., Chen, G., Lantz, R.C., Jolad, S.D., Sólyom, A.M., Timmermann, B.N., Turmeric extracts containing curcuminoids prevent experimental rheumatoid arthritis (2006) J Nat Prod, 69 (3), pp. 351-355. , Mar, PMID: 16562833Chaves, H.V., Ribeiro R De, A., De Souza, A.M., Rodrigues E Silva, A.A., Gomes, A.S., Vale, M.L., Bezerra, M.M., Brito, G.A., Experimental model of zymosan-induced arthritis in the rat temporomandibular joint: Role of nitric oxide and neutrophils (2011) J Biomed Biotechnol, 2011, p. 707985Keystone, E.C., Schorlemmer, H.U., Pope, C., Allison, A.C., Zymosan-induced arthritis: A model of chronic proliferative arthritis following activation of the alternative pathway of complement (1977) Arthritis Rheum, 20 (7), pp. 1397-1401. , Sep-Oct, PMID: 911357Gegout, P., Gillet, P., Terlain, B., Netter, P., Zymosan induced arthritis in rats II. Effects of antiinflammatory drugs (1995) Life Sci, 56 (20), pp. PL389-PL394. , PMID: 7723595Berner, J., Gabay, C., Best practice use of corticosteroids in rheumatoid arthritis (2014) Rev Med Suisse, 10 (421), pp. 603-608. , Mar 12, PMID: 24701713Narendhirakannan, R.T., Limmy, T.P., Anti-inflammatory and anti-oxidant properties of Sida rhombifolia stems and roots in adjuvant induced arthritic rats (2012) Immunopharmacol Immunotoxicol, 34 (2), pp. 326-336. , AprAggarwal, B.B., Sundaram, C., Malani, N., Ichikaw, H., Curcumin: The Indian solid gold (2007) Adv Exp Med Biol, 595, pp. 1-75. , PMID: 17569205Anand, P., Kunnumakkara, A.B., Newman, R.A., Aggarwal, B.B., Bioavailability of curcumin: Problems and promises (2007) Mol Pharm, 4 (6), pp. 807-818. , Nov-Dec, PMID: 17999464Taty Anna, K., Elvy Suhana, M.R., Faizah, O., Hamzaini, A.H., Anti-inflammatory effect of Curcuma longa (turmeric) on collagen-induced arthritis: An anatomico-radiological study (2011) Clin Ter, 162 (3), pp. 201-207. , PMID: 21717043Shishodia, S., Sethi, G., Aggarwal, B.B., Curcumin: Getting back to the roots (2005) Ann N Y Acad Sci, 1056, pp. 206-217. , Nov, PMID: 16387689Samuhasaneeto, S., Thong-Ngam, D., Kulaputana, O., Suyasunanont, D., Klaikeaw, N., Curcumin decreased oxidative stress, inhibited NF- kappaB activation, and improved liver pathology in ethanol-induced liver injury in rats (2009) J Biomed Biotechnol, 2009, p. 981963Ramadan, G., Al-Kahtani, M.A., El-Sayed, W.M., Anti-inflammatory and anti-oxidant properties of Curcuma longa (turmeric) versus Zingiber officinale (ginger) rhizomes in rat adjuvant-induced arthritis (2011) Inflammation, 34 (4), pp. 291-301. , AugPark, C., Moon, D.O., Choi, I.W., Choi, B.T., Nam, T.J., Rhu, C.H., Kwon, T.K., Choi, Y.H., Curcumin induces apoptosis and inhibits prostaglandin E(2) production in synovial fibroblasts of patients with rheumatoid arthritis (2007) Int J Mol Med, 20 (3), pp. 365-372. , Sep, PMID: 17671742Mun, S.H., Kim, H.S., Kim, J.W., Ko, N.Y., Kim, D.O.K., Lee, B.Y., Kim, B., Choi, W.S., Oral administration of curcumin suppresses production of matrix metalloproteinase (MMP)-1 and MMP-3 to ameliorate collagen- induced arthritis: Inhibition of the PKCdelta/JNK/c-Jun pathway (2009) J Pharmacol Sci, 111 (1), pp. 13-21. , Sep, PMID: 19763044Moon, D.O., Kim, M.O., Choi, Y.H., Park, Y.M., Kim, G.Y., Curcumin attenuates inflammatory response in IL-1beta-induced human synovial fibroblasts and collagen-induced arthritis in mouse model (2010) Int Immunopharmacol, 10 (5), pp. 605-610. , MayLantz, R.C., Chen, G.J., Solyom, A.M., Jolad, S.D., Timmermann, B.N., The effect of turmeric extracts on inflammatory mediator production (2005) Phytomedicine, 12 (6-7), pp. 445-452. , Jun, PMID: 16008121Srimal, R.C., Dhawan, B.N., Pharmacology of diferuloyl methane (curcumin), a non-steroidal antiinflammatory agent (1973) J Pharm Pharmacol, 25 (6), pp. 447-452. , Jun, PMID: 4146582Baker, C.L., Jr., Ferguson CM. Future treatment of osteoarthritis (2005) Orthopedics, 28 (2), pp. s227-s234. , Feb, PMID: 15747611Banerjee, M., Tripathi, L.M., Srivastava, V.M., Puri, A., Shukla, R., Modulation of inflammatory mediators by ibuprofen and curcumin treatment during chronic inflammation in rat (2003) Immunopharmacol Immunotoxicol, 25 (2), pp. 213-224. , May, PMID: 12784914Banji, D., Pinnapureddy, J., Banji, O.J., Saidulu, A., Hayath, M.S., Synergistic activity of curcumin with methotrexate in ameliorating Freund’s Complete Adjuvant induced arthritis with reduced hepatotoxicity in experimental animals (2011) Eur J Pharmacol, 668 (1-2), pp. 293-298. , Oct 1Funk, J.L., Frye, J.B., Oyarzo, J.N., Kuscuoglu, N., Wilson, J., McCaffrey, G., Stafford, G., Timmermann, B.N., Efficacy and mechanism of action of turmeric supplements in the treatment of experimental arthritis (2006) Arthritis Rheum, 54 (11), pp. 3452-3464. , Nov, PMID: 17075840Jancinová, V., Perecko, T., Nosál, R., Kostálová, D., Bauerová, K., Drábiková, K., Decreased activity of neutrophils in the presence of diferuloylmethane (curcumin) involves protein kinase C inhibition (2009) Eur J Pharmacol, 612 (1-3), pp. 161-166. , Jun 10Joe, B., Rao, U.J., Lokesh, B.R., Presence of an acidic glycoprotein in the serum of arthritic rats: Modulation by capsaicin and curcumin (1997) Mol Cell Biochem, 169 (1-2), pp. 125-134. , Apr, PMID: 908963

A retrospective analysis of colorectal serrated lesions from 2005 to 2014 in a single center: importance of the establishment of diagnostic patterns

sem informaçãoSerrated colorectal lesions are increasingly recognized as an important process in the development of colorectal cancer. Endoscopic and histological diagnosis may be difficult, and knowledge of the serrated lesions is important for the establishment of stsem informaçãosem informaçãosem informaçã

Paraestomal hernia with gastric outlet obstruction: a case report and literature review

sem informaçãoAn 69-year-old obese woman was submitted to an abdominoperineal resection (APR) with left side end colostomy to treat a synchronic sigmoid and middle rectum cancer. Six months after APR, she develop a PH with a progressive increase of the size. The patien7sem informaçãosem informaçãosem informaçã

Oxidative Stress And Changes In The Content And Pattern Of Tissue Expression Of β-catenin Protein In Diversion Colitis

Objective: The aim of this study is to verify if oxidative stress is related to changes in content and pattern of β-catenin protein expression in an experimental model of diversion colitis. Methods: Sixty Wistar rats were submitted to intestinal bypass. The animals were divided into three groups according to the sacrifice to take place in six, 12 and 18 weeks. For each group, five animals only underwent laparotomy (control). The presence of colitis was diagnosed by histological study, and its severity, by inflammation grading scale. Cellular oxidative stress was measured by comet assay. Tissue expression of β-catenin protein was analyzed by the immunohistochemistry and quantification of its tissue content by computerized morphometry. Statistical analysis was performed with the Student's t-test, median, Mann-Whitney, ANOVA and Kruskal-Wallis, adopting a significance level of 5% (p <0.05). Results: Colon segments without fecal stream developed colitis, which worsened with time of exclusion. Segments without fecal stream suffer higher levels of oxidative stress when compared to those with stream, and it worsens with time of exclusion. The levels of cellular oxidative stress are directly related to the degree of inflammation. The total content of β-catenin in segments without fecal stream reduces after six weeks, and does not vary thereafter. The content of β-catenin in the apical portion of the colon crypts decreases with time, whereas in the basal region, it increases. The total content of β-catenin is inversely related to the degree of inflammation and levels of tissue oxidative stress levels. Conclusion: There are changes in tissue content of E-cadherin and increased expression of β-catenin in proliferative regions of colonic crypts, related with oxidative tissue stress.324343358Pravda, J., Radical induction theory of ulcerative colitis (2005) World J Gastroenterol, 11 (16), pp. 2371-2384Gaudier, E., Hoebler, C., Physiological role of mucins in the colonic barrier integrity (2006) Gastroenterol Clin Biol, 30 (8-9), pp. 965-974Laukoetter, M.G., Nava, P., Nusrat, A., Role of the intestinal barrier in inflammatory bowel disease (2008) World J Gastroenterol, 14 (3), pp. 401-407Berkes, J., Viswananthan, V.K., Savkovic, S.D., Hecht, G., Intestinal epithelial responses to enteric pathogens: Effects on the tight junction barrier, iron transport, and inflammation (2003) Gut, 52 (3), pp. 439-451Clayburgh, D.R., Shen, L., Turner, J.R., A porous defense: The leaky epithelial barrier in intestinal disease (2004) Lab Invest, 84 (3), pp. 282-291Usami, Y., Chiba, H., Nakayama, F., Ueda, J., Matsuda, Y., Sawada, N., Reduced expression of claudin-7 correlates with invasion and metastasis in squamous cell carcinoma of the esophagus (2006) Hum Pathol, 37 (5), pp. 569-577Gumbiner, B., Stevenson, B., Grimaldi, A., The role of the cell adhesion molecule uvomorulin in the formation and maintenance of the epithelial junctional complex (1988) J Cell Biol, 107 (4), pp. 1575-1587Gumbiner, B.M., McCrea, P.D., Catenins as mediators of the cytoplasmic functions of cadherins (1993) J Cell Sci Suppl, 17, pp. 155-158Yeager, M., Unger, V.M., Falk, M.M., Synthesis, assembly and structure of gap junction intercellular channels (1998) Curr Opin Struct Biol, 8 (6), pp. 810-811Hynes, R.O., Zhao, Q., The evolution of cell adhesion (2000) J Cell Biol, 150 (2), pp. F89-F96Kypta, R., Bernfield, M., Burridge, K., Geiger, B., Goodenough, D., Humphries, M., Hynes, R., Yurchenco, P., Junções celulares, adesão celular e matriz extracelular (2006) Biologia Molecular Da Célula, pp. 1065-1125. , In: Alberts B, Johnson A, Lewis J, Raff M, Roberts K, Walter P. (eds.), Porto Alegre: ARTMEDDemetter, P., de Vos, M., van Damme, N., Baeten, D., Elewaut, D., Vermeulen, S., Focal up-regulation of E-cadherin catenin complex in inflamed bowel mucosa but reduced expression in ulcer-associated cell lineage (2000) Am J Clin Pathol, 114 (3), pp. 364-370Aust, D.E., Terdiman, J.P., Willenbucher, R.F., Chew, K., Ferrell, L., Florendo, C., Altered distribution of β-catenin, and its binding proteins E-cadherin and APC, in ulcerative colitisrelated colorectal cancers (2001) Mod Pathol, 14 (1), pp. 29-39Kucharzik, T., Walsh, S.V., Chen, J., Parkos, C.A., Nusrat, A., Neutrophil transmigration in inflammatory bowel disease is associated with differential expression of epithelial intercellular junction proteins (2001) Am J Pathol, 159 (6), pp. 2001-2009Laukoetter, M.G., Nava, P., Nusrat, A., Role of the intestinal barrier in inflammatory bowel disease (2008) World J Gastroenterol, 14 (3), pp. 401-407Gassler, N., Rohr, C., Schneider, A., Kartenbeck, J., Bach, A., Obermüller, N., Inflammatory bowel disease is associated with changes of enterocytic junctions (2001) Am J Physiol Gastrointest Liver Physiol, 281 (1), pp. G216-G228Ozawa, M., Ringwald, M., Kemler, R., Uvomorulin-catenin complex formation is regulated by a specific domain in the cytoplasmic region of the cell adhesion molecule (1990) Proc Natl Acad Sci USA, 87 (11), pp. 4246-4250Schmitz, H., Barmeyer, C., Fromm, M., Runkel, N., Foss, H.D., Bentzel, C.J., Altered tight junction structure contributes to the impaired epithelial barrier function in ulcerative colitis (1999) Gastroenterology, 116 (2), pp. 301-309Takahashi, M., Fukuda, K., Sugimura, T., Wakabayashi, K., Beta-catenin is frequently mutated and demonstrates altered cellular localization in azoxymethane-induced rat colon tumors (1998) Cancer Res, 58 (1), pp. 42-46Parrish, A.R., Catania, J.M., Orozco, J., Gandolfi, A.J., Chemically induced oxidative stress disrupts the E-cadherin/catenin cell adhesion complex (1999) Toxicol Sci, 51 (1), pp. 80-86Meyer, T.N., Schwesinger, C., Ye, J., Denker, B.M., Nigam, S.K., Reassembly of the tight junction after oxidative stress depends on tyrosine kinase activity (2001) J Biol Chem, 276 (25), pp. 22048-22055Dorudi, S., Sheffield, J.P., Poulsom, R., Northover, J.M., Hart, I.R., E-cadherin expression in colorectal cancer. An immunocytochemical and in situ hybridization study (1998) Am J Pathol, 142 (4), pp. 981-986Chen, J., Huang, X.F., The signal pathways in azoxymethane induced colon cancer and preventive implications (2009) Cancer Biol Ther, 8 (14), pp. 1313-1317Jankowski, J.A., Bedford, F.K., Boulton, R.A., Cruickshank, N., Hall, C., Elder, J., Alterations in classical cadherins associated with progression in ulcerative and Crohn's colitis (1998) Lab Invest, 78 (9), pp. 1155-1167Hermiston, M.L., Gordon, J.I., Inflammatory bowel disease and adenomas in mice expressing a dominant negative N-cadherin (1995) Science, 270 (5239), pp. 1203-1207Karayiannakis, A.J., Syrigos, K.N., Efstathiou, J., Valizadeh, A., Noda, M., Playford, R.J., Expression of catenins and E-cadherin during epithelial restitution in inflammatory bowel disease (1998) J Pathol, 185 (4), pp. 413-418Nollet, F., Berx, G., van Roy, F., The role of the E-cadherin/ catenin adhesion complex in the development and progression of cancer (1999) Mol Cell Biol Res Commun, 2 (2), pp. 77-85Sheehan, J.F., Brynjolfsson, G., Ulcerative colitis following hydrogen peroxide enema: Case report and experimental production with transient emphysema of colonic wall and gas embolism (1960) Lab Invest, 9, pp. 150-168Marques, L.H.S., Silva, C.M.G., Lameiro, T.M.M., Almeida, M.G., Cunha, F.L., Pereira, J.A., Avaliação dos níveis de peroxidação lipídica em células da mucosa cólica após aplicação de enemas com peróxido de hidrogênio: Estudo experimental em ratos (2010) Rev Bras Colo-proctol, 30 (3), pp. 272-280Martinez, C.A., Ribeiro, M.L., Gambero, A., Miranda, D.D., Pereira, J.A., Nadal, S.R., The importance of oxygen free radicals in the etiopathogenesis of diversion colitis in rats (2010) Acta Cir Bras, 25 (5), pp. 387-395Longatti, T.S., Acedo, S.C., de Oliveira, C.C., Miranda, D.D., Priolli, D.G., Ribeiro, M.L., Inflammatory alterations in excluded colon in rats: A comparison with chemically induced colitis (2010) Scand J Gastroenterol, 45 (3), pp. 315-324Damiani, C.R., Benetton, C.A., Stoffel, C., Bardini, K.C., Cardoso, V.H., Di Giunta, G., Oxidative stress and metabolism in animal model of colitis induced by dextran sulfate sodium (2007) J Gastroenterol Hepatol, 22 (11), pp. 1846-1851Liu, Q., Shimoyama, T., Suzuki, K., Umeda, T., Nakaji, S., Sugawara, K., Effect of sodium butyrate on reactive oxygen species generation by human neutrophils (2001) Scand J Gastroenterol, 36 (7), pp. 744-750Glotzer, D.J., Glick, M.E., Goldman, H., Proctitis and colitis following diversion of the fecal stream (1981) Gastroenterology, 80 (3), pp. 438-441Agarwal, V.P., Schimmel, E.M., Diversion colitis: A nutritional deficiency syndrome? 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MODELO DE TREINAMENTO DE DISSECÇÃO PÉLVICA LATERAL POR VIDEOLAPAROSCOPIA EM ANIMAIS (SUÍNOS)

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Adoção de técnicas laparoscópicas em ressecções colorretais entre cirurgiões brasileiros. Fatores limitantes que afetam a incorporação na prática diária

Routine adoption of laparoscopy in clinical practice and Medical Residency has not been widely evaluated in Brazil so far. Aim: To take an overview on the adoption and limitations concerning the use of laparoscopic techniques among Brazilian colorectal surgeons. Methods: A questionnaire was sent to 1870 SBCP filiated members, containing personal and professional data such as sex, age, length and local of practice, SBCP filliation, number of procedures, treatment of cancer and laparoscopy limitations. Results: 418 members (22.4%) sent their response (80% men and 20% women). 110 members (26.3%) affirmed they don't perform any laparoscopic procedure, while 308 (73.7%) have already adopted laparoscopy as a routine. An average number of 7.6 laparoscopic colorectal procedures were declared to be performed per month (1 to 40 procedures). Laparoscopic adoption rates were favourably influenced by young age members (46% vs. 28%) and affiliation to University hospitals (p = 0.01). Conversely, surgeons from private clinic showed a greater tendency of no adoption. Among the 308 responders, 106 (34.4%) have already surpassed more than 100 laparoscopic cases, and 167 (54.2%) reported an experience of more than 50 operated patients. The group of surgeons not using minimally invasive techniques incriminated lack of training (73.6%) and laparoscopic instruments availability (27.3%) as the main reasons for no adoption. Conclusions: Adoption rate of laparoscopic techniques to treat colorectal diseases is still low (at least 17%). Future efforts should focus on providing supervised training, proctorship during the initial experience and help instrumental acquisition in centers willing to change their routine and perspectives3912732sem informaçãoDurante as últimas décadas, a incorporação de técnicas minimamente invasivas no tratamento de doenças colorretais testemunhou um progresso lento e firme, principalmente após o reconhecimento da segurança oncológica e melhor evolução. A adoção rotineira na prática clínica e na Residência Médica no Brasil ainda não amplamente avaliada até agora. O presente estudo visou avaliar a adoção e as limitações relativas ao uso de técnicas laparoscópicas entre cirurgiões colorretais brasileiros. Um questionário foi enviado a 1870 membros filiados à Sociedade Brasileira de Coloproctologia (SBCP) em 2006. As questões foram enviadas por email, incluindo dados pessoais (sexo, idade) e profissionais (tempo e local de prática, filiação à SBCP, número mensal de procedimentos laparoscópicos, tratamento de câncer e limitações para realizar laparoscopia na rotina. Entre os 1870 membros, 418 (22.4%) mandaram sua resposta, com uma maior participaçãoo de homens (80%) em comparação às mulheres (20%). A idade média foi de 43 (28–80) anos. A distribuição entre membros titulares e não titulares foi semelhantes (48% vs. 52%). As atividades profissionais foram desenvolvidas em clínica privada (84%), hospitais privados (73%), hospitais públicos (50%) e hospitais universitários (53%). Entre os que responderam (418), 110 (26.3%) não realizavam procedimentos laparoscópicos, enquanto 308 (73.7%) já haviam adotado o acesso laparoscópico rotineiramente na prática clínica. Um número médio de 7.6 procedimentos colorretais laparoscópicos são realizados por mês (1–40). Cerca de 13% dos cirurgiões iniciaram sua experiência laparoscópica diretamente com procedimentos colorretais, enquanto a maioria (87%) começaram por outros procedimentos no trato digestivo. A adoção da laparoscopia foi positivamente influenciada pela idade jovem dos membros (46% vs. 28%) e pela filiação a hospitais universitários (p = 0,01). Inversamente, cirurgiões trabalhando na prática privada demonstraram uma menor tendência em adotar o método. A maioria dos cirurgiões (93%) que adoraram a laparoscopia afirmou incluir pacientes com câncer colorretal em suas indicações operatórias. Entre os que responderam 106 (34,4%) já realizaram mais de 100 procedimentos laparoscópicos, e 167 (54,2%) reportaram experiência maior que 50 casos. Dentre aqueles que não adotaram técnicas minimamente invasivas, a falta de treinamento (73,6%) ou a indisponibilidade de instrumental laparoscópico (27,3%) foram incriminadas como os principais fatores limitantes. O índice de adoção de técnicas laparoscópicas no tratamento de doenças intestinais ainda é baixo (pelo menos 17%) entre cirurgiões colorretais brasileiros. Esforços futuros de nossa Sociedade Médica devem focar na provisão de treinamento supervisionado, na criação de oportunidades para preceptoria durante a experiência inicial e na obtenção de instrumental em centros que queiram mudar sua rotina e perspectivassem informaçã

Evaluation Of The Expression Of The Mgmt Gene In Normal And Neoplastic Tissue Of Patients With Colorectal Cancer [avaliação Da Expressão Do Gene Mgmt Nos Tecidos Normal E Neoplásico De Doentes Com Câncer Colorretal]

Objective: To evaluate the expression of tissue repair gene MGMT by comparing normal and neoplastic colonic mucosa in patients with colorectal cancer (CRC). Methods: We studied 44 patients with colorectal cancer confirmed by histopathology. We excluded patients suspected of belonging to families with hereditary colorectal cancer (HNPCC and FAP) and patients with cancer of the lower or medium rectum treated with neoadjuvant chemoradiotherapy. The MGMT gene expression was assessed by the technique of polymerase chain reaction in real time (RT-PCR). The comparison of results for MGMT gene expression between normal and neoplastic tissues was made by paired Student's t test, adopting a significance level of 5% (p A:T transitions (2005) Gut, 54 (6), pp. 797-802Zaidi, N.H., Pretlow, T.P., O'Riordan, M.A., Dumenco, L.L., Allay, E., Gerson, S.L., Transgenic expression of human MGMT protects against azoxymethane-induced aberrant crypt foci and G to A mutations in the K-ras oncogene of mouse colon (1995) Carcinogenesis., 16 (3), pp. 451-456Qian, X.C., Brent, T.P., Methylation hot spots in the 5' flanking region denote silencing of the O6-methylguanine-DNA methyltransferase gene (1997) Cancer Res., 57 (17), pp. 3672-3677Esteller, M., Risques, R.A., Toyota, M., Capella, G., Moreno, V., Peinado, M.A., Promoter hypermethylation of the DNA repair gene O(6)-methylguanine-DNA methyltransferase is associated with the presence of G:C to A:T transition mutations in p53 in human colorectal tumorigenisis (2001) Cancer Res., 61 (12), pp. 4689-4692Esteller, M., Toyota, M., Sanchez-Cespedes, M., Capella, G., Peinado, M.A., Watkins, D.N., Inactivation of the DNA repair gene O6-methylguanine-DNA methyltransferase by promoter hypermethylation is associated with G to A mutations in K-ras in colorectal tumorigenisis (2000) Cancer Res., 60 (9), pp. 2368-2371Qi, J., Zhu, Y.Q., Huang, M.F., Yang, D., Hypermethylation of CpG island in O6-methylguanine-DNA methyltransferase gene was associated with K-ras G to A mutation in colorectal tumor (2005) World J Gastroenterol, 11 (13), pp. 2022-2025Martinez, C.A.R., Priolli, D.G., Cardinalli, I.A., Pereira, J.A., Portes, A.V., Margarido, N.F., Influência da localização do tumor na expressão tecidual da proteína p53 em doentes com câncer colorretal: Estudo de 100 casos (2008) Rev Col Bras Cir., 35 (4), pp. 235-243Sanchez de Abajo, A., de la Hoya, M., van Puijenbroek, M., Godino, J., Díaz-Rubio, E., Morreau, H., Dual role of LOH at MMR loci in hereditary non-polyposis colorectal cancer? (2006) Oncogene., 25 (14), pp. 2124-2130Martinez, C.A.R., Cordeiro, A.T., Priolli, D.G., Miranda, D.D.C., Bartchewsky Júnior, W., Margarido, N.F., Avaliação da expressão tecidual do gene de reparo MLH1 e dos níveis de dano oxidativo ao DNA em doentes com câncer colorretal (2009) Rev bras colo-proctol., 29 (3), pp. 303-313Park, J.H., Kim, N.S., Park, J.Y., Chae, Y.S., Kim, J.G., Sohn, S.K., MGMT-535G>T polymorphism is associated with prognosis for patients with metastatic colorectal cancer treated with oxaliplatin-based chemotherapy (2010) J Cancer Res Clin Oncol., 136 (8), pp. 1135-114

Laparoscopic versus open restorative proctocolectomy for familial adenomatous polyposis

sem informaçãoThis study compared outcomes after laparoscopic (LAP) or conventional (open) total proctocolectomy with outcomes after ileal J-pouch anal anastomosis (IPAA) at a single institution. Methods: Charts from 133 familial adenomatous polyposis patients (1997-202814752sem informaçãosem informaçãosem informaçã