Nutrigenomics and immune function in fish : new insights from omics technologies

This study was funded by BBSRC grant BB/M026604/1.Peer reviewedPublisher PD

Seawater transfer alters the intestinal microbiota profiles of Atlantic salmon (Salmo salar L.)

This study was funded by a BBSRC Eastbio PhD studentship to CED and BBSRC grant BB/M026604/1. The authors wish to thank Ana Rita Sancho Silva for facilitating the sampling for the experiment. Furthermore we would like to express our gratitude to Ian and Alastair Fraser for their support at the SFF fish farms on the Isle of Mull.Peer reviewedPublisher PD

Cloning and characterisation of multiple ferritin isoforms in the Atlantic salmon (Salmo salar)

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NFAT5 genes are part of the osmotic regulatory system in Atlantic salmon (Salmo salar)

Acknowledgements This study was supported by a grant from the Biotechnology and Biological Sciences Research Council (BBSRC, BB/H008063/1), UK to DGH and SAM. Funding also came from Research Council Norway for project number 241016 for DGH and EJ. This work was carried out as part of a PhD thesis funded by the Marine Alliance of Science and Technology Scotland (MASTS).Peer reviewedPublisher PD

Transcriptomic responses in the fish intestine

Acknowledgements This study was funded by BBSRC grant BB/M026604/1, and UK Technology Strategy Board (TSB) grant 11974-81166. CED was funded by a BBSRC EastBio PhD studentship at University of Aberdeen. We are grateful to Chris Secombes, Helen Dooley and the two anonymous referees who made valuable comments on the earlier version of the manuscript.Peer reviewedPublisher PD

Environmental and physiological factors shape the gut microbiota of Atlantic salmon parr (Salmo salar L.)

This study was funded by the BBSRC Eastbio PhD studentship of CED and BBSRC grant BB/M026604/1. The authors wish to thank the team at the Centre for Genome Enabled Biology and Medicine (CGEBM, Aberdeen) for their help and support, especially Brennan Martin for technical support and Matthew Gemmel for bioinformatics support.Peer reviewedPublisher PD

The compositional and metabolic responses of gilthead seabream (Sparus aurata) to a gradient of dietary fish oil and associated n-3 long-chain PUFA content

The authors express their gratitude to the technical team at the BioMar Feed Trial Unit, Hirtshals, in particular, Svend Jørgen Steenfeldt for expert care of the experimental subjects, for training and supervision provided by laboratory staff at Nutrition Analytical Services and Molecular Biology at the Institute of Aquaculture, University of Stirling, UK. S. J. S. H’s. PhD was co-funded by BioMar and the Marine Alliance for Science and Technology Scotland. BioMar provided the experimental feeds, trial facilities and fish, and covered travel expenses. V. K. and J. T. designed and executed the nutritional trial and all authors contributed to planning the analyses. V. K., J. T. and S. J. S. H. carried out the sampling. O. M., D. R. T and S. A. M. M. supervised the lead author. M. B. B. provided training in molecular biology to S. J. S. H. who carried out all analytical procedures. S. J. S. H. analysed all of the data and prepared the manuscript. Subsequently the manuscript was shared between all authors who made amendments, contributions and recommendations. The authors declare that there are no conflicts of interestPeer reviewedPublisher PD

Gut Associated Lymphoid Tissue (GALT) primary cells and stable cell lines as predictive models for intestinal health in rainbow trout (Oncorhynchus mykiss)

Funding This work was funded by the University of Aberdeen. Acknowledgments This work was funded by Skretting AI and the University of Aberdeen. Technical support by Dr Dawn Shewring was greatly appreciated. We also thank Dr Tiehui Wang, Scottish Fish Immunology Research Centre for providing the rIL-1β.Peer reviewedPublisher PD

Development of an Efficient Genome Editing Method by CRISPR/Cas9 in a Fish Cell Line

This project was carried out during CD’s BBSRC Eastbio PIPS placement at Marine Scotland. The authors are grateful to Dr Milena Monte (University of Aberdeen) for help with the FACS analysis. The authors wish to thank Dr Filippo Del Bene (Neuronal Circuit Development, Institut Curie) and Dr Wenbiao Chen (School of Medicine, Vanderbilt University) for the Addgene plasmids, #61051 and #47929, respectively, and Prof. Nancy C. Reich Marshall (Department of Molecular Genetics and Microbiology, Stony Brooks University) for the plasmid pmEGFP-N1.Peer reviewedPublisher PD