The clinical utility of the AUSDRISK tool in assessing change in type 2 diabetes risk in overweight/obese volunteers undertaking a healthy lifestyle intervention

The objective of this study was to assess the clinical utility of the AUSDRISK tool for determining risk of Type 2 diabetes mellitus (T2DM). In this secondary analysis from the HealthTrack study, the AUSDRISK tool was applied to data from overweight/obese volunteers completing a lifestyle intervention trial. Participants were volunteer residents of the Illawarra region recruited in 2014-2015. From 377 trial participants (BMI 25-40 kg/m2, 25-54 yr), 161 provided data required for measurement of AUSDRISK, collected at 0 and 12 months. They had been randomised to one of two lifestyle interventions (±a healthy food sample, 30 g walnuts/day, I and IW) delivered by dietitians, or a control intervention (C) delivered by nurse practitioners. HbA1c measures were considered for comparison. At baseline the AUSDRISK score indicated n = 83 (51.5%) were at high risk of T2DM within 5 years (≥12 points). After 12 months the proportion scored as high risk significantly decreased in the IW group (51.5% vs 33.3%; p = 0.005), but not I (51.2% vs 39.0%; p = 0.063) or C group (51.9% vs 38.9%; p = 0.065). By comparison, HbA1c measures indicated high risk in n = 24 (17%) of 139 participants at baseline and borderline non-significant changes over time in the randomised groups. In conclusion, the AUSDRISK tool has reasonable clinical utility in identifying T2DM risk in clinical samples of overweight/obese individuals

The Trypanosoma cruzi enzyme TcGPXI is a glycosomal peroxidase and can be linked to trypanothione reduction by glutathione or tryparedoxin.

Trypanosoma cruzi glutathione-dependent peroxidase I (TcGPXI) can reduce fatty acid, phospholipid, and short chain organic hydroperoxides utilizing a novel redox cycle in which enzyme activity is linked to the reduction of trypanothione, a parasite-specific thiol, by glutathione. Here we show that TcGPXI activity can also be linked to trypanothione reduction by an alternative pathway involving the thioredoxin-like protein tryparedoxin. The presence of this new pathway was first detected using dialyzed soluble fractions of parasite extract. Tryparedoxin was identified as the intermediate molecule following purification, sequence analysis, antibody studies, and reconstitution of the redox cycle in vitro. The system can be readily saturated by trypanothione, the rate-limiting step being the interaction of trypanothione with the tryparedoxin. Both tryparedoxin and TcGPXI operate by a ping-pong mechanism. Overexpression of TcGPXI in transfected parasites confers increased resistance to exogenous hydroperoxides. TcGPXI contains a carboxyl-terminal tripeptide (ARI) that could act as a targeting signal for the glycosome, a kinetoplastid-specific organelle. Using immunofluorescence, tagged fluorescent proteins, and biochemical fractionation, we have demonstrated that TcGPXI is localized to both the glycosome and the cytosol. The ability of TcGPXI to use alternative electron donors may reflect their availability at the corresponding subcellular sites

Delivery of Family-Based Treatment for Adolescent Anorexia Nervosa in a Public Health Care Setting: Research Versus Non-Research Specialty Care.

Comparing evidence-based psychotherapy (EBP) to usual care typically demonstrates the superiority of EBPs, although this has not been studied for eating disorders EBPs such as family-based treatment (FBT). The current study set out to examine weight outcomes for adolescents with anorexia nervosa who received FBT through a randomized clinical research trial (RCT, n = 54) or non-research specialty care (n = 56) at the same specialist pediatric eating disorder service. Weight was recorded throughout outpatient treatment (up to 18 sessions over 6 months), as well as at 6- and 12-month follow-up. Survival curves were used to examine time to weight restoration [greater than 95% median body mass index (mBMI)] as predicted by type of care (RCT vs. non-research specialty care), baseline clinical and demographic characteristics, and their potential interaction. Results did not indicate a significant main effect for type of care, but there was a significant effect for baseline weight (p = .03), such that weight restoration was achieved faster across both treatment types for those with a higher initial %mBMI. These data suggest that weight restoration achieved in non-research specialty care FBT was largely similar to that achieved in a controlled research trial. Clinical Trial Registration:http://www.anzctr.org.au/, identifier ACTRN12610000216011

Effects of prenatal exposure to endocrine disruptors and toxic metals on the fetal epigenome

Exposure to environmental contaminants during pregnancy has been linked to adverse outcomes at birth and later in life. The link between prenatal exposures and latent health outcomes suggests that these exposures may result in long-term epigenetic reprogramming. Toxic metals and endocrine disruptors are two major classes of contaminants that are ubiquitously present in the environment and represent threats to human health. In this review, we present evidence that prenatal exposures to these contaminants result in fetal epigenomic changes, including altered global DNA methylation, gene-specific CpG methylation and microRNA expression. Importantly, these changes may have functional cellular consequences, impacting health outcomes later in life. Therefore, these epigenetic changes represent a critical mechanism that warrants further study

Structure and Function of the Stigma in Relation to the Germinative Requirements of the Pollen in the Easter Lily

Stigma of Easter Lily is papillate. Over the surface of the papillae a mucilaginous layer is formed and from this mucilaginous layer the pollen absorbs the requisite amount of water for germination. The papillae and nearly all cells of the stigma previous to the opening of the flower contain much starch which is transported from cell to cell chiefly in the form of dextrin. As the starch disappears in the papillae the mucilage appears on the outside of their walls. The pollen germinates on almost any media or in almost any solution that furnishes the required amount of water

Our 25+ Year Journey To AACSB Accreditation

How does a publicly supported university with a primary focus on teaching, achieve accreditation from the premier agency and service organization for business schools all over the world, the Association to Advance Collegiate Schools of Business (AACSB)? Our journey began in 1978 when a college department hired a Dean, became a separate business school and had a vision to become a successful leader in business education. Our goal was achieved and culminated on January 7, 2005 with the accreditation of our undergraduate and graduate business programs. The who, what, where, when and why of our 25+ year journey will be provided. Particular emphasis will be placed on our numerous hurdles and how the goal was achieved such as funding availability; curriculum reform; raising publication rates, and why it took so long to get accredited

NGC 5548: THE AGN ENERGY BUDGET PROBLEM AND THE GEOMETRY OF THE BROAD-LINE REGION AND TORUS

We consider in detail the spectral energy distribution (SED) and multi-wavelength variability of NGC 5548. Comparison with the SEDs of other AGNs implies that the internal reddening of NGC 5548 is E(B-V) = 0.17 mag. The extinction curve is consistent with the mean curve of other AGNs found by Gaskell & Benker, but inconsistent with an SMC-type reddening curve. Because most IR emission originates exterior to the broad-line region (BLR), the SED seen by the inner BLR is different from that seen by the outer BLR and from the earth. The most likely BLR covering factor is ~ 40% and it is not possible to get an overall BLR covering factor of less than 20%. This requires that the BLR is not spherically symmetric and that we are viewing through a hole. Line-continuum variability transfer functions are consistent with this geometry. The covering factor and geometry imply that near the equatorial plane the BLR covering approaches 100%. The spectrum seen by the outer regions of the BLR and by the torus is thus modified by the absorption in the inner BLR. This shielding solves the problem of observed BLR ionization stratification being much greater than implied by photoionization models. The BLR obscuration also removes the problem of the torus covering factor being greater than the BLR covering factor, and gives consistency with the observed fraction of obscured AGNs. The flux reduction at the torus also reduces the problem of AGN dust-reverberation lags giving sizes smaller than the dust-sublimation radii

Environmental contaminants and microRNA regulation: Transcription factors as regulators of toxicant-altered microRNA expression

MicroRNAs (miRNAs) regulate gene expression by binding mRNA transcripts and inhibiting translation and/or inducing degradation of the associated transcripts. Expression levels of miRNAs have been shown to be altered in response to environmental toxicants, thus impacting cellular function and influencing disease risk. Transcription factors (TFs) are known to be altered in response to environmental toxicants and play a critical role in the regulation of miRNA expression. To date, environmentally-responsive TFs that are important for regulating miRNAs remain understudied. In a state-of-the-art analysis, we utilized in silico bioinformatic analysis to characterize potential transcriptional regulators of environmentally-responsive miRNAs. Using the miRStart database, genomic sequences of promoter regions for all available human miRNAs (n=847) were identified and promoter regions were defined as −1000/+500 base pairs from the transcription start site. Subsequently, the promoter region sequences of environmentally-responsive miRNAs (n=128) were analyzed using enrichment analysis to determine overrepresented TF binding sites (TFBS). While most (56/73) TFs differed across environmental contaminants, a set of 17 TFs was enriched for promoter binding among miRNAs responsive to numerous environmental contaminants. Of these, one TF was common to miRNAs altered by the majority of environmental contaminants, namely SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin, subfamily A, member 3 (SMARCA3). These identified TFs represent candidate common transcriptional regulators of miRNAs perturbed by environmental toxicants