Organic vs Conventional Suckling Lamb Production: Product Quality and Consumer Acceptance

Samples of suckling lambs (n=40) of two breeds reared under conventional and organic conditions were analysed to asses physico-chemical characteristics, including instrumental texture, and nutritional quality in terms of fatty acid composition. Consumer acceptance was also studied using the home-use test. Results revealed that organic suckling lamb meat is healthier as shown by the lower saturated fatty acid levels, the higher polyunsaturated fatty acid contents and the higher 6/3 ratiko. The organic meat had lower instrumental hardness, received higher scores in all sensory parameters, and had statistically better fat sensation and higher ratings for overall liking. These results lend support to the notion among consumers that organic products are healthier and tastier

Consumer Appreciation of Carcass Quality of Organic vs Conventional Suckling Lamb Production

Carcass characteristics of sucking lambs (n= 40) of two breeds reared under conventional and organic conditions were analysed including objective and subjective parameters for fatness and conformation, meat and fat colour. Consumer acceptance was also studied using the home-use test. Results showed that the characteristics of the carcass of suckling lamb were similar for both types of production systems pointing out that organic production system did not affect fatness or muscle development. However, organic meat was darker (higher L* and a* values) probably related with the higher amount of exercise, although fat was not more yellow. In contrast consumers did not consider organic meat darker and there were not significant differences in appearance related with the similar conformation. These results reflect that consumer perceive organic meat as at least as good as conventional production not only regarding environmental quality but also regarding carcass quality

Thy-1 (CD90)-Induced Metastatic Cancer Cell Migration and Invasion Are β3 Integrin-Dependent and Involve a Ca²⁺/P2X7 Receptor Signaling Axis.

Cancer cell adhesion to the vascular endothelium is an important step in tumor metastasis. Thy-1 (CD90), a cell adhesion molecule expressed in activated endothelial cells, has been implicated in melanoma metastasis by binding to integrins present in cancer cells. However, the signaling pathway(s) triggered by this Thy-1-Integrin interaction in cancer cells remains to be defined. Our previously reported data indicate that Ca <sup>2+</sup> -dependent hemichannel opening, as well as the P2X7 receptor, are key players in Thy-1-α <sub>V</sub> β <sub>3</sub> Integrin-induced migration of reactive astrocytes. Thus, we investigated whether this signaling pathway is activated in MDA-MB-231 breast cancer cells and in B16F10 melanoma cells when stimulated with Thy-1. In both cancer cell types, Thy-1 induced a rapid increase in intracellular Ca <sup>2+</sup> , ATP release, as well as cell migration and invasion. Connexin and Pannexin inhibitors decreased cell migration, implicating a requirement for hemichannel opening in Thy-1-induced cell migration. In addition, cell migration and invasion were precluded when the P2X7 receptor was pharmacologically blocked. Moreover, the ability of breast cancer and melanoma cells to transmigrate through an activated endothelial monolayer was significantly decreased when the β <sub>3</sub> Integrin was silenced in these cancer cells. Importantly, melanoma cells with silenced β <sub>3</sub> Integrin were unable to metastasize to the lung in a preclinical mouse model. Thus, our results suggest that the Ca <sup>2+</sup> /hemichannel/ATP/P2X7 receptor-signaling axis triggered by the Thy-1-α <sub>V</sub> β <sub>3</sub> Integrin interaction is important for cancer cell migration, invasion and transvasation. These findings open up the possibility of therapeutically targeting the Thy-1-Integrin signaling pathway to prevent metastasis

Linking optical and molecular signatures of dissolved organic matter in the Mediterranean Sea

Original research paperDissolved organic matter (DOM) plays a key role in global biogeochemical cycles and experiences changes in molecular composition as it undergoes processing. In the semi-closed basins of the oligotrophic Mediterranean Sea, these gradual molecular modifications can be observed in close proximity. In order to extend the spatial resolution of information on DOM molecular composition available from ultrahigh resolution mass spectrometry in this area, we relate this data to optical (fluorescence and absorption spectroscopy) measurements. Covariance between molecular formulae signal intensities and carbon-specific fluorescence intensities was examined by means of Spearman’s rank correlations. Fifty two per cent of the assigned molecular formulae were associated with at least one optical parameter, accounting for 70% of the total mass spectrum signal intensity. Furthermore, we obtained significant multiple linear regressions between optical and intensity-weighted molecular indices. The resulting regression equations were used to estimate molecular parameters such as the double bond equivalent, degradation state and occurrence of unsaturated aliphatic compounds from optical measurements. The statistical linkages between DOM molecular and optical properties illustrate that the simple, rapid and cost-efficient optical spectroscopic measurements provide valuable proxy information on the molecular composition of open ocean marine DOM.y the project HOTMIX (grant number CTM2011-30010-C02-MAR) and the project FERMIO (MINECO, CTM2014-57334-JIN), both co-financed with FEDER fundsVersión del editor2,92

Control biológico In Vitro de nematodos agalladores (Meloidogyne sp.) a base de quitosano y fluensulfone

El nematodo agallador (Meloidogyne sp.) tiene importancia económica potencial en diversos cultivos, en su combate casi siempre se han utilizado diferentes productos de agroquímicos, por lo que se necesita evaluar nuevos productos que sean una mejor alternativa en su manejo y en el cuidado al medio ambiente. En el presente trabajo se evaluó la eficacia biológica de diferentes extractos del quitosano un biopolímero natural con efecto nematicida y regulador de poblaciones de estos fitonematodos y Fluensulfone un nematicida de amplio espectro, el estudio se realizó In vitro, se colectaron muestras de suelo y se extrajeron los nematodos mediante el método de embudo de Baermann, se colocaron en cajas petri en diluciones con 30-35 nematodos por unidad experimental, en donde para su control se utilizó quitosano extraído de langostino de rio y quitosano comercial a diferentes concentraciones (0. 25, 0.50, 0.75), así mismo un nematicida comercial con extracto de quitosano Nematrol Plus , esto comparado con otros nematicidas comerciales Ditera (Myrothecium verrucaria  ),  Nemover  (Extracto  de  pino)  y  Nimitz  (Fluensulfone)  .Se  encontró  que  el  efecto nematicida del quitosano en dosis media (0.50) fue mejor posterior a las 24 horas después de la aplicación con una mortalidad de 95.83 a 100 % de nematodos,  y una eficacia del 100 %, esto comparado con los nematicidas comerciales

Safety of switching from intravenous to subcutaneous rituximab during first-line treatment of patients with non-Hodgkin lymphoma: the Spanish population of the MabRella study

Rituximab is a standard treatment for non-Hodgkin diffuse large B-cell (DLBCL) and follicular (FL) lymphomas. A subcutaneous formulation was developed to improve the resource use of intravenous rituximab, with comparable efficacy and safety profiles except for increased administration-related reactions (ARRs). MabRella was a phase IIIb trial to assess the safety of switching from intravenous to subcutaneous administration of rituximab during first-line induction/maintenance for DLBCL or FL, focusing on ARRs. Efficacy, satisfaction and quality of life were also assessed. Patients received subcutaneous rituximab plus standard induction chemotherapy for DLBCL or FL for 4–7 cycles, and/or every 2 months maintenance monotherapy for FL for 6–12 cycles. The study included 140 patients: DLBCL, n = 29; FL, n = 111. Ninety-five percent of patients experienced adverse events, reaching grade ≥3 in 38 6% and were serious in 30 0%. AARs occurred in 48 6%, mostly (84 9%) at the injection site, with only 2 1% of patients reaching grade 3. The end-of-induction complete/unconfirmed complete response rate was 69 6%. After a median follow-up of 33 5 months, median disease-/event-/progression-free and overall survivals were not attained. The Rituximab Administration Satisfaction Questionnaire showed improvements in overall satisfaction and the EuroQoL-5D a good quality-of-life perception at induction/maintenance end. Therefore, switching to subcutaneous rituximab showed no new safety issues and maintained efficacy with improved satisfaction and quality of life

Dissolved Organic Matter (DOM) in the open Mediterranean Sea. I. Basin-Wide distribution and drivers of chromophoric DOM

Original research articleChromophoric dissolved organic matter (CDOM) in the open Mediterranean Sea (MedSea) is barely documented, remaining the basin–wide patterns in intermediate and deep waters still enigmatic. Here, full–depth distributions of CDOM absorption coefficients and spectral slopes recorded during the HOTMIX 2014 cruise are presented and their respective environmental drivers resolved. General Additive Models (GAMs) in surface waters and Optimum MultiParameter (OMP) water mass analysis in deep waters were applied. In the surface, apparent oxygen utilisation (AOU), a proxy to cumulative net community respiration, explained most of the variability of dissolved organic carbon (DOC) and the absorption coefficient at 254 nm (a254), whereas the absorption coefficient at 325 nm (a325), and the spectral slopes were mostly explained by potential temperature, a proxy to stratification and solar radiation, indicating that both water column stability and photobleaching may drive the variability of the UV–A absorbing CDOM components. In deep waters, the effect of water mass mixing and basin–scale mineralization were discerned from local mineralization processes. Water mass mixing and basin–scale mineralization contributed more substantially to explain the variability of DOC, a254 and a325 (82–91%) than the variability of the spectral slopes (35–64%). Local mineralization processes indicate that DOC and CDOM play a more relevant role in the carbon cycle in the Eastern (EastMed) than in the Western (WestMed) Mediterranean: whereas DOC contributed to 66 ± 10% of the oxygen demand in the EastMed, it represented only 24 ± 4% in the WestMed. Independently of basins and layers, a254 revealed as an excellent proxy to the concentration of DOC in the MedSea. Also, the unexpected inverse relationship of a325 with AOU indicates that the consumption of the UV–A absorbing CDOM fraction prevails over their productionSpanish Ministry of Education and Culture, Spanish Ministry of Economy and Competitiviness, FEDER, CSIC, University of GranadaVersión del editor3,26

Dissolved organic matter (DOM) in the open Mediterranean Sea. II: Basin-wide distribution and drivers of fluorescent DOM

Research articleFluorescent dissolved organic matter (FDOM) in the Mediterranean Sea was analysed by excitation–emission matrix (EEM) spectroscopy and parallel factor (PARAFAC) analysis during the cruise HOTMIX 2014. A 4–component model, including 3 humic–like and 1 protein–like compounds, was obtained. To decipher the environmental factors that dictate the distributions of these components, we run generalized additive models (GAMs) in the epipelagic layer and an optimum multiparametric (OMP) water masses analysis in the meso– and bathypelagic layers. In the epipelagic layer, apparent oxygen utilization (AOU) and temperature presented the most significant effects on the variability of the marine humic-like peak M fluorescence, suggesting that its distribution was controlled by the net community respiration of organic matter and photobleaching. On the contrary, the variability of the soil humic-like peak E and the protein–like peak T fluorescence was explained mainly by the prokaryotic heterotrophic abundance, which decreased eastwards. In the meso– and bathypelagic layers, water mass mixing and basin–scale mineralization processes explained >72% and 63% of the humic–like and protein–like fluorescence variability, respectively. When analysing the two basins separately, the OMP model offered a better explanation of the distribution of fluorescence in the eastern Mediterranean Sea, as expected from the reduced biological activity in this ultra–oligotrophic basin. Furthermore, while western Mediterranean deep waters display the usual trend in the global ocean (increase of humic–like fluorescence and decrease of protein–like fluorescence with higher AOU values), the eastern Mediterranean deep waters presented an opposite trend. Different initial fluorescence intensities of the water masses that mix in the eastern basin, with Adriatic and Aegean origins, seem to be behind this contrasting pattern. The analysis of the transect–scale mineralization processes corroborate this hypothesis, suggesting a production of humic–like and a consumption of protein–like fluorescence in parallel with water mass ageing. Remarkably, the transect–scale variability of the chromophoric dissolved organic matter (CDOM) absorbing at the excitation wavelength of the humic–like peak M indicates an unexpected loss with increasing AOU, which suggests that the consumption of the non–fluorescent fraction of CDOM absorbing at that wavelength exceeded the production of the fluorescent fraction observed hereProject HOTMIX (reference CTM2011–30010–C02 01–MAR and 02–MAR), co–financed with FEDER funds (re ference BES–2012–056175) ; the project MODMED from CSIC (PIE, 201730E020) and the project FERMIO (MINECO, CTM2014–57334–JIN), co–financed with FEDER fundsVersión del editor3,26

Perrault syndrome with neurological features in a compound heterozygote for two TWNK mutations : Overlap of TWNK-related recessive disorders

Altres ajuts: This research was supported with cofounding from the European Regional Development Fund (ERDF), "A way to make Europe") (to IdC); S2017/BMD‑3721‑RAREGENOMICS‑CM from the Consejería de Educación e Investigación de la Comunidad de Madrid (to MAMP).Background: Perrault syndrome is a rare autosomal recessive disorder that is characterized by the association of sensorineural hearing impairment and ovarian dysgenesis in females, whereas males have only hearing impairment. In some cases, patients present with a diversity of neurological signs. To date, mutations in six genes are known to cause Perrault syndrome, but they do not explain all clinically-diagnosed cases. In addition, the number of reported cases and the spectra of mutations are still small to establish conclusive genotype-phenotype correlations. Methods: Affected siblings from family SH19, who presented with features that were suggestive of Perrault syndrome, were subjected to audiological, neurological and gynecological examination. The genetic study included genotyping and haplotype analysis for microsatellite markers close to the genes involved in Perrault syndrome, whole-exome sequencing, and Sanger sequencing of the coding region of the TWNK gene. Results: Three siblings from family SH19 shared similar clinical features: childhood-onset bilateral sensorineural hearing impairment, which progressed to profound deafness in the second decade of life; neurological signs (spinocerebellar ataxia, polyneuropathy), with onset in the fourth decade of life in the two females and at age 20 years in the male; gonadal dysfunction with early cessation of menses in the two females. The genetic study revealed two compound heterozygous pathogenic mutations in the TWNK gene in the three affected subjects: c.85C>T (p.Arg29∗), previously reported in a case of hepatocerebral syndrome; and a novel missense mutation, c.1886C>T (p.Ser629Phe). Mutations segregated in the family according to an autosomal recessive inheritance pattern. Conclusions: Our results further illustrate the utility of genetic testing as a tool to confirm a tentative clinical diagnosis of Perrault syndrome. Studies on genotype-phenotype correlation from the hitherto reported cases indicate that patients with Perrault syndrome caused by TWNK mutations will manifest neurological signs in adulthood. Molecular and clinical characterization of novel cases of recessive disorders caused by TWNK mutations is strongly needed to get further insight into the genotype-phenotype correlations of a phenotypic continuum encompassing Perrault syndrome, infantile-onset spinocerebellar ataxia, and hepatocerebral syndrome

On the use of evolutionary time series analysis for segmenting paleoclimate data

Recent studies propose that different dynamical systems, such as climate, ecological and financial systems, among others, present critical transition points named to as tipping points (TPs). Climate TPs can severely affect millions of lives on Earth so that an active scientific community is working on finding early warning signals. This paper deals with the development of a time series segmentation algorithm for paleoclimate data in order to find segments sharing common statistical patterns. The proposed algorithm uses a clustering-based approach for evaluating the solutions and six statistical features, most of which have been previously considered in the detection of early warning signals in paleoclimate TPs. Due to the limitations of classical statistical methods, we propose the use of a genetic algorithm to automatically segment the series, together with a method to compare the segmentations. The final segments provided by the algorithm are used to construct a prediction model, whose promising results show the importance of segmentation for improving the understanding of a time series