766 research outputs found
Virtual Issue: Molecular Sensors
MartĂnez-Máñez, R. (2014). Virtual Issue: Molecular Sensors. ChemistryOpen. 3(6):232-232. doi:10.1002/open.201402070S2322323
Microalgae degradation follow up by voltammetric electronic tongue, impedance spectroscopy and NMR spectroscopy
[EN] Microalgae play a fundamental role in aquatic primary production and the food chain. They are a recognized source of fatty acids and fatty acid-based lipids of potential interest in the preparation of functional health products, biofuels and renewable chemicals. The exploitation of this bioresource requires a fine monitoring of each production stage. The aim of this work is the microalgae degradation follow up after the concentration stage at the end of the production process by voltammetric electronic tongue, impedance spectroscopy and H-1 NMR spectroscopy. Microalgae samples were allowed to progress along time (from 1 to 23 days). At scheduled selected times, voltammetry, impedance spectroscopy and H-1 NMR measurements were performed. Multivariate analysis was carried out on these data by PLSR. A model calculated in a training set was then applied to a set of validation to predict the time of evolution. For the three techniques good results in prediction for the validation set were obtained (R-2/RMSEP of 0.961/1.51, 0.956/1.67 and 0.969/1.25 respectively for impedance, voltammetry and NMR spectroscopy). The three techniques were sensitive to the evolution of the microalgae samples. The detection of metabolical changes in the H-1 NMR spectra is also included. This proof of concept could be the basis for future development of rapid and robust strategies for quality control on microalgae production plants.Authors gratefully acknowledge the financial support of MAT2015-64139-C4-1-R and MAT2015-64139-C4-3-R (MINECO/FEDER, UE) and PROMETEOII/2014/047 projects. BUGGYPOWER, S.L. is acknowledged for the financial support and for providing the samples. U26 Nanbiosis is acknowledged by the NMR measurements.MartĂnez-Bisbal, M.; CarbĂł-Mestre, N.; MartĂnez-Máñez, R.; Bauzá, J.; Alcañiz Fillol, M. (2019). Microalgae degradation follow up by voltammetric electronic tongue, impedance spectroscopy and NMR spectroscopy. Sensors and Actuators B Chemical. 281:44-52. https://doi.org/10.1016/j.snb.2018.10.069S445228
A Versatile New Paradigm for the Design of Optical Nanosensors Based on Enzyme-Mediated Detachment of Labeled Reporters: The Example of Urea Detection
"This is the peer reviewed version of the following article: Llopis-Lorente, Antoni, Reynaldo Villalonga, M. Dolores Marcos, RamĂłn MartĂnez-Máñez, and FĂ©lix SancenĂłn. 2018. A Versatile New Paradigm for the Design of Optical Nanosensors Based on Enzyme‐Mediated Detachment of Labeled Reporters: The Example of Urea Detection. Chemistry A European Journal 25 (14). Wiley: 3575 81. doi:10.1002/chem.201804706. , which has been published in final form at https://doi.org/10.1002/chem.201804706. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving."[EN] Here, a new bio-inspired nanoarchitectonics approach for the design of optical probes is presented. It is based on nanodevices that combine 1) an enzymatic receptor subunit, 2) a signaling subunit (consisting of a labeled reporter attached to a silica surface), and 3) a mechanism of communication between the two sites based on the production of chemical messengers by the enzymatic subunit, which induces the detachment of the reporter molecules from the silica surface. As a proof of concept, a urea nanosensor based on the release of Alexa-Fluor-647-labeled oligonucleotide from enzyme-functionalized Janus gold-mesoporous-silica nanoparticles (Au-MSNPs) was developed. The Janus particles were functionalized on the silica face with amino groups to which the labeled oligonucleotides were attached by electrostatic interactions, whereas the gold face was used for grafting urease enzymes. The nanodevice was able to release the fluorescent oligonucleotide through the enzyme-mediated hydrolysis of urea to ammonia and the subsequent deprotonation of amino groups on the silica face. This simple nanodevice was applied for the fluorometric detection of urea in real human blood samples and for the identification of adulterated milk. Given the large variety of enzymes and reporter species that could be combined, this is a general new paradigm that could be applied to the design of a number of optical probes for the detection of target analytes.A.L.-L. is grateful to "La Caixa" Banking Foundation for his Ph.D. fellowship. The authors thank to the Spanish Government (MINECO Projects MAT2015-64139-C4-1, AGL2015-70235-C2-2-R, CTQ2014-58989-P and CTQ2015-71936-REDT) and the Generalitat Valencia (Projects PROMETEOII/2014/047, PROMETEO2018/024) for support. The Comunidad de Madrid (S2013/MIT-3029, Programme NANOAVANSENS) is also gratefully acknowledged.Llopis-Lorente, A.; Villalonga, R.; Marcos MartĂnez, MD.; MartĂnez-Máñez, R.; SancenĂłn Galarza, F. (2019). A Versatile New Paradigm for the Design of Optical Nanosensors Based on Enzyme-Mediated Detachment of Labeled Reporters: The Example of Urea Detection. Chemistry - A European Journal. 25(14):3575-3581. https://doi.org/10.1002/chem.201804706S35753581251
Sucrose-responsive intercommunicated janus nanoparticles network
[EN] Inspired by biological systems, the development of artificial nanoscale materials that communicate over a short distance is still at its early stages. This work shows a new example of a cooperating system with intercommunicated devices at the nanoscale. The system is based on the new sucrose-responsive Janus gold-mesoporous silica (Janus Au-MS) nanoparticles network with two enzyme-powered nanodevices. These nanodevices involve two enzymatic processes based on invertase and glucose oxidase, which are anchored on the Au surfaces of different Janus Au-MS nanoparticles, and N-acetyl-L-cysteine and [Ru(bpy)(3)](2+) loaded as chemical messengers, respectively. Sucrose acts as the INPUT, triggering the sequential delivery of two different cargoes through the enzymatic control. Nanoscale communication using abiotic nanodevices is a developing potential research field and may prompt several applications in different disciplines, such as nanomedicine.Financial support was provided by the Spanish Ministry of Economy and Competitiveness (MINECO Projects CTQ2017-87954-P). D.V. thanks MICINN for the Juan de la Cierva fellowship (IJC2018-035658-I). R.M.-M. thanks the Generalitat Valenciana (Project PROMETEO2018/024).JimĂ©nez-Falcao, S.; Torres, D.; MartĂnez-Ruiz, P.; Vilela, D.; MartĂnez-Máñez, R.; Villalonga, R. (2021). Sucrose-responsive intercommunicated janus nanoparticles network. Nanomaterials. 11(10):1-11. https://doi.org/10.3390/nano11102492111111
Zeolites synthesis using as a raw material the aluminium anodizing sludges
[EN] There were found, by atomic absorption and scanning electronic microscopy techniques, concentrations in between of 25,3% and 39,45% of aluminium respectively, in the anodized aluminium sludge on an industry that operates in Ecuador. Besides there were identified minor concentrations of Na, Mg, Ca and Si. Therefore the objective of the present research was to investigate, at the laboratory level, the feasibility of using these industrial residues as a source of precursors for the zeolites synthesis.
The chemical composition of a fixed 20 g of sludge was adjusted by using a sodium hydroxide solutions in a varying concentrations of 0,5 M, 1,0 M, 1,5 M, 2,0 M, 2,5 M and 3,0 M in a mixture with hydrated Na2SiO3 in a constant amount added to the raw material. Molar ratio SiO2/Al2O was kept constant at 1,24 value, whereas the Na2O/Al2O3; Na2O/SiO2 and H2O/Na2O were varied as sodium hydroxide solutions changed in concentration.
The processes of precursor's dilution, aging and zeolite crystallization were carried out a constant temperature of 100 degrees C for a 48 hours reaction time.
By the X-ray diffraction technique, the synthesis of the Na-P1 GIS zeolite [Na-6(H2O)(12)][Si10Al6O32] was identified, with three types of mineralogy. Besides the zeolites ACP-1 and Sodalite Octahydrate were identified. The electronic images show a product with a well-defined morphology. The optimum conditions for the synthesis were the 1.5 M concentration of the caustic soda solution, and molar ratios of 1,24 SiO2/Al2O3; 0,61 Na2O/Al2O3; 2,02 SiO2/Na2O; 15,51 H2O/Na2O and 7,68 H2O/SiO2.
At higher concentrations of the sodium hydroxide solution, the same zeolites were obtained, as evidenced by X-ray diffractograms, but the electronic images of the products show deformed crystals, possibly due to the excess of sodium hydroxide.[ES]
Se encontrĂł, por absorciĂłn atĂłmica y escaneado electrĂłnico de barrido concentraciones entre 25,3% y 39,45 % de aluminio, además de sodio, magnesio, calcio y silicio en los lodos de anodizado de aluminio de una industria que opera en Ecuador. Por lo tanto el objetivo del presente trabajo fue investigar a nivel de laboratorio, la posibilidad de utilizar estos residuos como fuente de precursores en la sĂntesis de zeolitas.
Utilizando una soluciĂłn de hidrĂłxido de sodio en concentraciones 0,5M, 1,0 M, 1,5 M, 2,0 M, 2,5 M y 3,0 M en mezcla con Na2SiO3 hidratado, se ajustĂł la composiciĂłn quĂmica de 20 g de muestra de lodo a una constante de relaciĂłn molar SiO2/Al2O3 de 1,24 para todas las pruebas y variando las relaciones molares de Na2O/Al2O3; Na2O/SiO2 y H2O/ Na2O, conforme a la variaciĂłn de la concentraciĂłn de la soluciĂłn de hidrĂłxido de sodio. Se mantuvo constante la temperatura de fusiĂłn de precursores, envejecimiento y cristalizaciĂłn de la zeolita a 100°C y tiempo de reacciĂłn de 48 horas.
Mediante la tĂ©cnica de difracciĂłn de rayos X, se identificĂł la sĂntesis de la zeolita Na-P1 GIS , con tres tipos de mineralogĂa, además las zeolitas ACP-1 y Sodalita Octahidrato. Las imágenes electrĂłnicas muestran un producto con una morfologĂa bien definida. Las mejores condiciones del resultado fueron la concentraciĂłn 1,5 M de la soluciĂłn de sosa y relaciones molares 1,24 de SiO2/Al2O3; 0,61 de Na2O/Al2O3; 2,02 de SiO2/Na2O; 15,51, de H2O/ Na2O y 7,68 de H2O/ SiO2.
A mayores concentraciones de sosa se obtienen las mismas zeolitas, como lo demuestran los difractogramas de rayos X, pero las imágenes electrĂłnicas de los productos muestran cristales deformados, posiblemente por el exceso de sosa.Peñafiel-Villarreal, F.; MartĂnez-Máñez, R. (2019). Sintesis de Zeolitas utilizando como materia prima lodos de los procesos de anodizado de aluminio. TecnologĂa en Marcha. 32(3):12-23. https://doi.org/10.18845/tm.v32i3.4476S122332
Tratamiento con agonistas opiáceos y prolongación del intervalo QTc: una revisión sistemática
Fluorogenic Detection of Human Serum Albumin Using Curcumin-Capped Mesoporous Silica Nanoparticles
[EN] Mesoporous silica nanoparticles loaded with rhodamine B and capped with curcumin are used for the selective and sensitive fluorogenic detection of human serum albumin (HSA). The sensing mesoporous silica nanoparticles are loaded with rhodamine B, decorated with aminopropyl moieties and capped with curcumin. The nanoparticles selectively release the rhodamine B cargo in the presence of HSA. A limit of detection for HSA of 0.1 mg/mL in PBS (pH 7.4)-acetonitrile 95:5 v/v was found, and the sensing nanoparticles were used to detect HSA in spiked synthetic urine samples.This research was funded by the Spanish Government (RTI2018-100910-B-C41 (MCUI/FEDER, EU)) and the Generalitat Valenciana (PROMETEO 2018/024). I.O. was funded by Erasmus Mundus Programme, Action 2, Lot 1, Syria (predoctoral fellowship). S.M. was funded by Generalitat Valenciana (Santiago Grisolia fellowship).Otri, I.; Medaglia, S.; Aznar, E.; SancenĂłn Galarza, F.; MartĂnez-Máñez, R. (2022). Fluorogenic Detection of Human Serum Albumin Using Curcumin-Capped Mesoporous Silica Nanoparticles. Molecules. 27(3):1-9. https://doi.org/10.3390/molecules270311331927
Chromo-fluorogenic probes for beta-galactosidase detection
[EN] beta-Galactosidase (beta-Gal) is a widely used enzyme as a reporter gene in the field of molecular biology which hydrolyzes the beta-galactosides into monosaccharides. beta-Gal is an essential enzyme in humans and its deficiency or its overexpression results in several rare diseases. Cellular senescence is probably one of the most relevant physiological disorders that involve beta-Gal enzyme. In this review, we assess the progress made to date in the design of molecular-based probes for the detection of beta-Gal both in vitro and in vivo. Most of the reported molecular probes for the detection of beta-Gal consist of a galactopyranoside residue attached to a signalling unit through glycosidic bonds. The beta-Gal-induced hydrolysis of the glycosidic bonds released the signalling unit with remarkable changes in color and/or emission. Additional examples based on other approaches are also described. The wide applicability of these probes for the rapid and in situ detection of de-regulation beta-Gal-related diseases has boosted the research in this fertile fieldR.M laboratory members received the financial support from the Spanish Government (project RTI2018-100910-B-C41) and the Generalitat Valenciana (project PROMETEO 2018/024). B.L-T. received support from the Spanish Ministry of Economy for their PhD grants (FPU15/02707). J. F.-B received fellowship (CD19/00038)Lozano-Torres, B.; Blandez, JF.; SancenĂłn Galarza, F.; MartĂnez-Máñez, R. (2021). Chromo-fluorogenic probes for beta-galactosidase detection. Analytical and Bioanalytical Chemistry. 413(9):2361-2388. https://doi.org/10.1007/s00216-020-03111-8S236123884139Fernandes P. Enzymes in food processing: a condensed overview on strategies for better biocatalysts. Enzyme Res. 2010;2010:86253–73.Likidlilid A, Patchanans N, Peerapatdit T, Sriratanasathavorn C. 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Glycosyltransferase and glycosidase activities in ovarian cancer
Avidin-gated mesoporous silica nanoparticles for signal amplification in electrochemical biosensor
[EN] We report herein a novel sensing strategy for electrochemical biosensors, by using mesoporous silica nanoparticles loaded with the redox probe methylene blue and capped with an avidin/imminobiotin stimulus-responsive gate-like ensemble as signal amplification element. As a proof of concept, an aptasensor for carcinoembryonic antigen (CEA) was constructed by attaching a biotin and thiol-functionalized anti-CEA DNA hairpin aptamer on gold nanoparticles modified carbon screen-printed electrodes. The biosensing approach relied on the unfolding of the aptamer molecule after specific recognition of CEA, unmasking the biotin residue and allowing further association with the avidin-capped mesoporous nanocarrier. Incubation with H2SO4 trigger the release of the encapsulated redox probe allowing the detection of the cancer biomarker from 1.0 pg/mL to 160 ng/mL.Financial support from the Spanish Ministry of Economy and Competitiveness (projects CTQ2014-58989-P, CTQ2015-71936-REDT, CTQ2017-87954-P and RTI2018-100910-B-C41, fellowship BES-2015-073565 to SJF) and the Generalitat Valencia (Project PROMETEO/2018/024) are gratefully acknowledged.Jimenez-Falcao, S.; Parra-Nieto, J.; PĂ©rez-Cuadrado, H.; MartĂnez-Máñez, R.; MartĂnez-Ruiz, P.; Villalonga, R. (2019). Avidin-gated mesoporous silica nanoparticles for signal amplification in electrochemical biosensor. Electrochemistry Communications. 108:1-4. https://doi.org/10.1016/j.elecom.2019.1065561410
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