Creación de un Portafolio Gráfico de Marcas Representativas a Nivel Mundial, Nacional y Local

Adj, Cd. "PORTAFOLIO DE MARCAS" 88T00008Mediante el análisis de marcas se desarrolló un Portafolio Gráfico que constituirá una valiosa fuente referencial que utilizada en el proceso de creación de identidad corporativa permitirá potenciar su calidad. El diseño del portafolio está definido previo a un estudio realizado mediante método inductivo para la investigación de la información, el método experimental fue utilizado para el análisis de las marcas y del cómo cada persona reaccionaba frente a ellas. Para la elaboración del portafolio se utilizó programas graficadores como Adobe: Ilustrador y photoshop, que ayudaron en el diseño general y contenidos del impreso, utilizando técnicas de análisis gráficos, semánticos, culturas, estilos gráficos colores presentes dentro de cada marca, además de técnicas de escogitamiento de marcas de un universo de 210 marcas tanto mundiales, nacionales como locales del que se trabajó con una muestra de 53 unidades. El portafolio gráfico desarrollado es de mucha utilidad en la Escuela de Diseño Gráfico, sirviendo como una guía funcional para mejorar los posibles diseños que se podrían realizar, además se logró la asimilación de los contenidos en un 99% de manera atractiva y a la vez muy práctica. Este documento servirá como una ficha de consulta para profesionales, estudiantes y docentes de diseño Gráfico contribuyendo con su desarrollo creativo recomendándose que este portafolio repose en la biblioteca de la Institución para su consulta y utilización

Accessible Interfaces for Educational Multimedia Contents

Proceedings of: Workshop on Advanced Learning Technologies for Disabled and Non-Disabled People (WALTD 2008), July 1st-July 5th, 2008, Santander, Cantabria, Spain, in conjunction with the 8th IEEE International Conference on Advanced Learning Technologies (ICALT 2008)The use of technology is growing in every field of education, and not only in the education of disabled students but also as a learning resource for everybody. The teachers are more and more introducing these digital contents in their lessons and there are many resources related to learning on internet.. If an equal access to these resources is guaranteed, then we can avoid students feeling that their learning capacity is limited due to a possible inaccessibility to them. Inclusive methodologies have to be followed to reach these objectives applying the standards such as Web Content Accessibility Guideline (WCAG) and rules of accessibility in the design and development of web pages, technical supporting, software, author tools, etc. This papers describes a practical case with two accessible interfaces of multimedia resources implemented with Synchronized Multimedia Integration Language (SMIL.)Publicad

Accessible Interface for Multimedia Presentation in Inclusive Education

[Poster] 12th Annual SIGCSE Conference on Innovation and Technology in Computer Science Education (ITiCSE'07), 25-27th June, 2007, Dundee (Scotland)Among the technological solutions to achieve an inclusive education, the educative electronic accessible resources providing content adaptability is an alternative which reduces discrimination. It also favors the inclusion with right equalities which can be used by students regardless of their specific need and use contexts. Following this line of adaptability of contents on the web, a practical case [1] has been launched: an accessible interface of a multimedia resource with caption and audiodescription that offers the user the control over the decision of which sound and/or visual alternatives he wants to be introduced to. This resource has been designed with Universal Design criteria which are found in the Inclusive Design methodological frameworkPublicad

The Effects of Native Shrub, Fencing, and Acorn Size on the Emergence of Contrasting Co-Occurring Oak in Mediterranean Grazed Areas

[ENG]Research Highlights: The regeneration of Quercus species is usually very difficult in many oak woodlands transformed by livestock farming. Some studies have reported that shrubs can facilitate regeneration. However, the strength of interaction may vary depending on, among other factors, the shrub species and the stress tolerance of the oak species. Moreover, further studies are necessary to clarify the relative importance of the two facilitation mechanisms in the same community. Background and Objectives: Cytisus multiflorus (L’Her.) Sweet is a predominant shrub species in the Mediterranean grazed open-oak-woodlands found in the central west of the Iberian Peninsula (bioclimatic limit) and is present with Quercus pyrenaicaWilld and Quercus ilex subsp. ballota Samp trees. Thus, we assessed the effect of these native shrubs and acorn size, and the effect of excluding large herbivores, on the seedling emergence of two contrasting co-occurring Quercus species under a bioclimatic limit. Materials and Methods: A manipulative field experiment was carried out considering four treatments as a combination of shrubs (shrub/no-shrub) and fence (fenced/open) factors. A total of twenty plots, five replicates for each treatment were available. In each plot, 20 acorns were sown: 10 acorns (5 small and 5 large) for each Quercus species. Acorn emergence was recorded during the first four years following the sowing. Results: Seedling emergence took place mostly in the spring of the first year after sowing. The presence of shrub was the main significant factor and incremented the emergence of both Q. ilex and Q. pyrenaica. The effect of the fence depended on the Quercus species considered, improving only the emergence of Q. pyrenaica. A negative effect with the small acorns was detected but only for Q. pyrenaica. In all treatments, Q. ilex emerged more than Q. pyrenaica. Conclusions: C. multiflorus had a clear facilitative effect on the seedling emergence of Q. ilex and Q. pyrenaica, which was much greater than the physical effect that acorn size and excluding large herbivores had. As such, this native shrub may have a key role in oak regeneration in Mediterranean grazed areas. Furthermore, in these areas of contact between marcescent and sclerophyllous Quercus species, Q. ilex currently emerges more than Q. pyrenaica. This could be indicative of a shift towards more xeric climatic conditions, which could lead to a change in the dominant tree species in the future. However, this change could be modulated by the effects of native shrub and large herbivores

Development of an in-capillary derivatization method by CE for the determination of chiral amino acids in dietary supplements and wines

A fast in-capillary derivatization method by CE with 6-aminoquinolyl-N-hydroxysuccinimidyl\ud carbamate was developed for the first time for the determination of\ud amino acid enantiomers (arginine, lysine, and ornithine) in dietary supplements and\ud wines. Because of the initial current problems due to the formation of precipitates into\ud the capillary during the derivatization reaction, a washing step with an organic solvent as\ud DMSO between injections was necessary. Different approaches were also investigated to\ud enhance the sensitivity of detection. A derivatization procedure, where plugs of ACN,\ud derivatizing agent (10mM 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate), and\ud sample in borate (1:1 v/v) were injected in tandem (2, 3, and 6 s, respectively, at 50 mbar),\ud was selected because it enabled to obtain the most sensitive and reproducible results.\ud Appropriate analytical characteristics (linearity, LOD and LOQ, precision, absence of\ud matrix interferences, and accuracy) were obtained for this method. Finally, the optimized\ud method was successfully applied to the determination of the enantiomers of arginine,\ud lysine, and ornithine in food samples of different complexities (dietary supplements and\ud wines).The authors thank the Ministry of Education and Science (Spain) and the Comunidad Autónoma de Madrid (Spain) for research projects CTQ2006-03849/BQU and S-0505/AGR- 0312, respectively. Carmen Garcı´a-Ruiz thanks the Ministry of Science and Technology (Spain) for the Ramon y Cajal program (RYC-2003-001). A.B.M.-G. thanks the University of Alcala for her pre-doctoral contract

Modeling Splicing Variants Amenable to Antisense Therapy by Use of CRISPR-Cas9-Based Gene Editing in HepG2 Cells

The field of splice modulating RNA therapy has gained new momentum with FDA approved antisense-based drugs for several rare diseases. In vitro splicing assays with minigenes or patient-derived cells are commonly employed for initial preclinical testing of antisense oligonucleotides aiming to modulate splicing. However, minigenes do not include the full genomic context of the exons under study and patients' samples are not always available, especially if the gene is expressed solely in certain tissues (e.g. liver or brain). This is the case for specific inherited metabolic diseases such as phenylketonuria (PKU) caused by mutations in the liver-expressed PAH gene.Herein we describe the generation of mutation-specific hepatic cellular models of PKU using CRISPR/Cas9 system, which is a versatile and easy-to-use gene editing tool. We describe in detail the selection of the appropriate cell line, guidelines for design of RNA guides and donor templates, transfection procedures and growth and selection of single-cell colonies with the desired variant , which should result in the accurate recapitulation of the splicing defectThis book is based upon work from COST Action DARTER (CA 17103), supported by COST (European Cooperation in Science and Technology)

Effect of the forest-mine boundary form on woody colonization and forest expansion in degraded ecosystems

[ENG]We evaluated the ecological significance of the boundary form between two patches with contrasting vegetation (mine grassland and adjacent forest) on woody colonization and forest expansion in open-cast coal mines in Northern Spain. Woody colonization and browsing traces were measured on three mine sites, along 24 transects that were laid out perpendicular to the forest-mine boundary and classified according to their shape (concave, convex, straight). Mine sites were colonized from the close forest by woody species, whose colonization intensity depends on the boundary form. The overall colonization intensity decreased with increasing distance to the forest and differed depending on the boundary form. The more intense colonization was found in concave boundaries and the strongest decrease in convex boundaries close to the forest, whereas straight boundaries showed an intermediate colonization pattern. Concave boundaries reached higher woody cover in the basal strata of the mines than convex (up to 2 m) or straight boundaries (up to 1 m) from 11 m to the forest edge, mainly by the presence of dense patches of Cytisus scoparius (L.) Link, with a scattered overstory of Genista florida L. These shrubs might reduce the browsing intensity and act as nurse plants facilitating the establishment of Quercus petraea (Matt.) Liebl. in mine areas at greater distances from the forest edge. The forest-mine boundary form does not affect the forest vertical structure that is homogenous and does not help explain the woody colonization pattern in the mines. We conclude that edge characteristics have a strong potential to be used in the restoration of native forests based on natural processes. The implications of our results for sessile oak (Quercus petraea (Matt.) Liebl.) forest expansion along edges in fragmented Mediterranean forest landscapes were discussed

Toward an equal opportunity web: applications, standards, and tools that increase accessibility

This paper discusses about rich Internet applications and supporting technology, such as JavaScript and Flash. RIAs can offer an interactive experience closer to that of a desktop application.This research work is supported by the Research Network MAVIR (S2009/TIC-1542) (www.mavir.net), project TIN2007- 67407-C03-01, and the Spanish Center of Captioning and Audio Description (www.cesya.com)

Enantiomeric separation of ornithine in complex mixtures of amino acids by EKC with off-line derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate

A new analytical methodology was developed by EKC enabling the fast enantiomeric separation of\ud Ornithine in complex mixtures of amino acids. A previous derivatization step with 6-aminoquinolyl-Nhydroxysuccinimidyl\ud carbamate (AQC) was achieved to enable the sensitive UV detection of amino acids\ud as well as to make possible their interaction with the CDs employed as chiral selectors. A dual CD system\ud containing an anionic and a neutral CD in phosphate buffer at acid pH showed a high resolving power\ud allowing the enantiomeric separation of 18 protein amino acids and Orn. The method was applied to the\ud analysis of fermented foods to investigate the extent of the presence of Orn enantiomers.Authors thank the Ministry of Education of Science (Spain) and\ud the Comunidad Autonoma de Madrid (Spain) for research projects CTQ2006-03849/BQU and S-0505/AGR-0312, respectively. Carmen Garcıa-Ruiz thanks the Ministry of Science and Technology (Spain) for the Ramon y Cajal program (RYC-2003-001). Ana Belen Martínez \ud Giron and Elena Domínguez-Vega thank the University of Alcala for their pre-doctoral grants

Thermal characterization and diffusivity of two mono-component epoxies for transformer insulation

The main aim of this study is the thermal characterization of an organic insulation. This insulation is a compound of two mono-component epoxy resins: Epoxylite® primer and Elmotherm® varnish. A mono-component epoxy resin usually needs a high temperature to cure; through differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA), non-isothermal curves are obtained, allowing the estimation of activation energies of curing and decomposition processes respectively. If Model Free Kinetic (MFK) is used from DSC curves, it is possible to simulate isothermal curves at different temperatures and times, plotting activation energy as a function of the conversion degree. The simulation from TGA curves can be used to estimate lifetime of the resins and compare them following the Toop method. DSC also allows measurement of thermal conductivity, the melting peak of metallic gallium being used for this measurement. Finally, water diffusion in resins is studied. Currently, the Materials Performance research group of UC3M is working on the European project named "Essial", where this organic insulation is used to protect the windings and the whole transformer from the environment. The results obtained will be used to determinate the optimal operating range for this insulator, demonstrating that both epoxies are required to achieve the insulating performance of the transformer and long curing times are required for full curing of Epoxylite®.This research was funded by European Union's Horizon 2020 research and innovation programme under grant agreement No 766437 (ESSIAL project)