Haemophilus parasuis

The expression of chemokines (CCL-2 and CXCL-8) and cytokines (IL-1α, IL-1β, IL-6, TNF-α, and IL-10) was evaluated by RT-qPCR in colostrum-deprived pigs vaccinated and challenged with Haemophilus parasuis serovar 5. Two vaccines containing native proteins with affinity to porcine transferrin (NPAPTim and NPAPTit) were tested, along with two control groups: one inoculated with PBS instead of antigen (challenge group (CHG)), and another one nonimmunized and noninfected (blank group). The use of NPAPTim and NPAPTit resulted in complete protection against H. parasuis (no clinical signs and/or lesions), and both vaccines were capable of avoiding the expression of the proinflammatory molecules to levels similar to physiological values in blank group. However, overexpression of all proinflammatory molecules was observed in CHG group, mainly in the target infection tissues (brain, lungs, and spleen). High expression of CCL-2, CXCL-8, IL-1α, IL-1β, and IL-6 can be considered one of the characteristics of H. parasuis infection by serovar 5

Inhomogeneity correction of magnetic resonance images by minimization of intensity overlapping

Proceeding of: IEEE 2003 International Conference on Image Processing (ICIP), Barcelona, Spain, 14-17 Sept. 2003This work presents a new algorithm (NIC; Non uniform Intensity Correclion) for the correction of intensity inhomogeneities in magnetic resonance images. The algorithm has been validated by means of realistic phantom images and a set of 24 real images. Evaluation using previously proposed phantom images for inhomogeneity correction algorithms allowed us to obtain results fully comparable to the previous literature on the topic. This new algorithm was also compared, using a real image dataset, to other widely used methods which are freely available in the Internet (N3, SPM'99 and SPM2). Standard quality criteria have been used for determining the goodness of the different methods. The new algorithm showed better results removing the intensity inhomogeneities and did not produce degradation when used on images free from this artifact

Ejercicio interlaboratorio de bioensayos marinos para la evaluación de la calidad ambiental de sedimentos costeros. IV. Ensayo de toxicidad sobre sedimento con crustáceos anfípodos

9 páginas, 2 figuras, 3 tablas.[EN] Interlaboratory variability was studied for the bioassay using crustacean amphipods. Nine series of data were obtained from different laboratories using four different species (Ampelisca brevicornis, Corophium volutator, Corophium multisetosum and Microdeutopus gryllotalpa), all previously used for sediment toxicity assessment in Spain. Results were studied for interlaboratory variability and according to different confounding factors. The coefficients of variation were similar to those previously reported for this bioassay and sample classification was homogeneous among the laboratories that successfully completed the exercise. No significant tendencies related to test species were identified and it seems that the factor most critically affecting test results and the classification of samples is storage time before testing.[ES] Se estudió la precisión interlaboratorio del bioensayo con crustáceos anfípodos. Se recogieron nueve series de datos procedentes de distintos laboratorios europeos y de cuatro especies distintas (Ampelisca brevicornis, Corophium volutator, Corophium multisetosum y Microdeutopus gryllotalpa), todas ellas usadas previamente para la caracterización de la toxicidad de sedimentos en España. Esos resultados se estudiaron para evaluar la variabilidad interlaboratorio de acuerdo a las diferentes especies y los distintos tiempos de almacenamiento de las muestras antes del ensayo. Los resultados mostraron coeficientes de variación similares a los encontrados en estudios previos y permitieron una clasificación homogénea de las muestras entre los laboratorios que completaron el ejercicio con éxito. No se identificaron tendencias significativas debidas al uso de distintas especies y, al parecer, el factor que puede afectar más críticamente la clasificación de las muestras y, por lo tanto, el uso de los resultados para la toma de decisiones, es el tiempo de almacenamiento de los sedimentos previamente al desarrollo del ensayo.This study was carried out under a joint research project between CEDEX and the University of Cádiz. Part of the work was funded by the Spanish Ministry of Science and Technology REN2002_01699/TECNO). The first author was supported by a grant (FPI) from the Spanish Ministry of Education and Science.Peer reviewe

Cap Formation Induced by Concanavalin A in Pathogenic Free-living Amoebae

Morphological differences in cap formation were found when trophozoites of different free-living amoebae were treated with the lectin Concanavalin A, which resulted in a rapid redistribution of certain surface components to form small clusters and membrane-folded structures of diverse sizes. Acanthamoeba castellanii, Acanthamoba polyphaga and Naegleria lovaniensis exhibited characteristic caps, however, in A. castellanii this structure was larger and included several folds of the plasma membrane; furthermore, some of these caps had vacuoles containing a fi bro granular content. In contrast, the caps formed by A. polyphaga and N. lovaniensis lacked vacuoles. Regarding Naegleria fowleri, the trophozoites did not produce a defi ned cap, and only small patches of lectin-bound surface receptor complexes were observed at one pole of the cell body. In the free-living amoebae studied, it was not possible to correlate the shape and size of cap with pathogenicity

Dietary avian proteins are comparable to soybean proteins on the atherosclerosis development and fatty liver disease in apoe-deficient mice

Background and aim: The type and amount of dietary protein has become a topic of re-newed interest in light of their involvement in metabolic diseases, atherosclerosis and thrombosis. However, little attention has been devoted to the effect of avian proteins despite their wide human consumption. The aim was to investigate the influence of chicken and turkey as sources of protein compared with that of soybean on atherosclerosis and fatty liver disease. Methods and results: To this purpose, male and female Apoe-deficient were fed purified Western diets differing in their protein sources for 12 weeks. After this period, blood, liver, aortic tree and heart base samples were taken for analyses of plasma lipids and atherosclerosis. Plasma triglycerides, non-esterified fatty acids, esterified cholesterol levels and radical oxygen species in lipoproteins changed depending on the diet and sex. Females consuming the turkey protein-containing diet showed decreased athero-sclerotic foci, as evidenced by the en face atherosclerosis analyses. The presence of macrophages and smooth muscle cells in plaques were not modified, and no changes were observed in hepatic lipid droplets in the studied groups either. Paraoxonase activity was higher in the group consuming turkey protein without sex differences, but only in females, it was significantly associated with aor-tic lesion areas. Conclusions: Compared to soybean protein, the consumption of avian proteins depending on sex resulted in similar or lower atherosclerosis development and comparable hepatic steatosis. © 2021 by the authors. Licensee MDPI, Basel, Switzerland

A novel Carcinoembryonic Antigen (CEA)-Targeted Trimeric Immunotoxin shows signifcantly enhanced Antitumor Activity in Human Colorectal Cancer Xenografts

Immunotoxins are chimeric molecules, which combine antibody specifcity to recognize and bind with high-afnity tumor-associated antigens (TAA) with the potency of the enzymatic activity of a toxin, in order to induce the death of target cells. Current immunotoxins present some limitations for cancer therapy, driving the need to develop new prototypes with optimized properties. Herein we describe the production, purifcation and characterization of two new immunotoxins based on the gene fusion of the anti-carcinoembryonic antigen (CEA) single-chain variable fragment (scFv) antibody MFE23 to α-sarcin, a potent fungal ribotoxin. One construct corresponds to a conventional monomeric single-chain immunotoxin design (IMTXCEAαS), while the other one takes advantage of the trimerbody technology and exhibits a novel trimeric format (IMTXTRICEAαS) with enhanced properties compared with their monomeric counterparts, including size, functional afnity and biodistribution, which endow them with an improved tumor targeting capacity. Our results show the highly specifc cytotoxic activity of both immunotoxins in vitro, which was enhanced in the trimeric format compared to the monomeric version. Moreover, the trimeric immunotoxin also exhibited superior antitumor activity in vivo in mice bearing human colorectal cancer xenografts. Therefore, trimeric immunotoxins represent a further step in the development of next-generation therapeutic immunotoxins

Chromospheric activity and rotation of FGK stars in the solar vicinity. An estimation of the radial velocity jitter

Context: Chromospheric activity produces both photometric and spectroscopic variations that can be mistaken as planets. Large spots crossing the stellar disc can produce planet-like periodic variations in the light curve of a star. These spots clearly affect the spectral line profiles and their perturbations alter the line centroids creating a radial velocity jitter that might contaminate" the variations induced by a planet. Precise chromospheric activity measurements are needed to estimate the activity-induced noise that should be expected for a given star. Aims: We obtain precise chromospheric activity measurements and projected rotational velocities for nearby (d < 25 pc) cool (spectral types F to K) stars, to estimate their expected activity-related jitter. As a complementary objective, we attempt to obtain relationships between fluxes in different activity indicator lines, that permit a transformation of traditional activity indicators, i.e, CaII H & K lines, to others that hold noteworthy advantages. Methods: We used high resolution (~50000) echelle optical spectra. To determine the chromospheric emission of the stars in the sample, we used the spectral subtraction technique. Rotational velocities were determined using the cross-correlation technique. To infer activity-related radial velocity (RV) jitter, we used empirical relationships between this jitter and the R'_HK index. Results: We measured chromospheric activity, as given by different indicators throughout the optical spectra, and projected rotational velocities for 371 nearby cool stars. We have built empirical relationships among the most important chromospheric emission lines. Finally, we used the measured chromospheric activity to estimate the expected RV jitter for the active stars in the sample.Comment: Accepted for publication in Astronomy & Astrophysic

SARS-CoV-2 outbreak on a Spanish mink farm: epidemiological, molecular, and pathological studies

Farmed minks have been reported to be highly susceptible to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and may represent a risk to humans. In this study, we describe the first outbreak of SARS-CoV-2 occurred on a mink farm in Spain, between June and July 2020, involving 92,700 animals. The outbreak started shortly after some farm workers became seropositive for SARS-CoV-2. Minks showed no clinical signs compatible with SARS-CoV-2 infection throughout the outbreak. Samples from 98 minks were collected for histopathological, serological, and molecular studies. Twenty out of 98 (20.4%) minks were positive by RT-qPCR and 82 out 92 (89%) seroconverted. This finding may reflect a rapid spread of the virus at the farm with most of the animals overcoming the infection. Additionally, SARS-CoV-2 was detected by RT-qPCR in 30% of brain samples from positive minks. Sequencing analysis showed that the mink sequences were not closely related with the other mink SARS-CoV-2 sequences available, and that this mink outbreak has its probable origin in one of the genetic variants that were prevalent in Spain during the first COVID-19 epidemic wave. Histological studies revealed bronchointerstitial pneumonia in some animals. Immunostaining of viral nucleocapsid was also observed in nasal turbinate tissue. Farmed minks could therefore constitute an important SARS-CoV-2 reservoir, contributing to virus spread among minks and humans. Consequently, continuous surveillance of mink farms is needed