EVALUACIÓN DE LA PROGRAMACIÓN DEL RIEGO EN UNA COMUNIDAD DE REGANTES MEDIANTE BALANCE DE ENERGIA CON IMÁGENES LANDSAT 8

[EN] Surface Energy Balance Algorithms for Land (SEBAL) parametrized for citrus (Jiménez-Bello et al. 2015) was applied to an irrigation district during the irrigation season 2014. 22 irrigated orchards were selected and monthly ratios of ET calculated by FAO methodology (ETFAO), with no soil water limitations, and calculated ET by SEBAL (ETSEBAL ) were compared to the ratio of ETFAO and supplied volumes to each orchard (VOL). Linear and potential trends of ETSEBAL ETFAO-1 compared to ETFAO Vol-1 were determined. In every month there was a positive correlation for the two trends, showing that ETSEBAL in stressed crops is lower than ETFAO. The potential trend has shown best determination coefficients, since for overirrigated crops water percolates and is not registered in the surface energy balance model. Instead of further research is needed to know how pixel size and vegetation cover affect ETSEBAL, SEBAL is a methodology that can be used to estimate citrus water requirements for large irrigation districts,[ES] El modelo para para el cálculo de la evapotranspiración (ET) por balance de energía a nivel del suelo (SEBAL), una vez ha sido parametrizado para cítricos (Jiménez-Bello et al. 2015), ha sido aplicado a una comunidad de regantes durante la campaña de riego 2014. Entre las parcelas regadas se han seleccionado 22 y se han comparado mensualmente los ratios de la ET calculada mediante la metodología FAO (ETFAO), que lo hace para un cultivo sin limitaciones de agua en el suelo, y la ET calculada con SEBAL (ETSEBAL) frente al ratio de ETFAO y los volúmenes aportados a cada parcela (VOL). Se ha determinado la tendencia lineal y potencial de la comparación de ETSEBAL ETFAO-1 con ETFAO Vol-1 . En todos los meses ha existido correlación positiva de los datos para las dos tendencias, lo que viene a demostrar que ETSEBAL en cultivos estresados es menor que ETFAO. El modelo potencial ha mostrado mejores coeficientes de determinación, puesto que en cultivos sobreregados llega un límite en el que no se produce más ET y al percolar el agua, ésta no queda recogida en el balance de energía superficial. A falta de una investigación más detallada de cómo puede afectar el tamaño y la cubierta vegetal de la parcela, SEBAL es una metodología que puede ser utilizada para la estimación de las necesidades de riego en cítricos en comunidades de regantes.Este trabajo ha sido financiado por el proyecto FIGARO del Séptimo Programa Marco europeo. Los autores agradecen la ayuda proporcionada por el personal del Sector XI de Picassent.Jiménez Bello, MÁ.; Martínez Alzamora, F.; Martínez Gimeno, M.; Intrigliolo, D. (2015). EVALUACIÓN DE LA PROGRAMACIÓN DEL RIEGO EN UNA COMUNIDAD DE REGANTES MEDIANTE BALANCE DE ENERGIA CON IMÁGENES LANDSAT 8. En XXXIII CONGRESO NACIONAL DE RIEGOS. Valencia 16-18 junio de 2015. Editorial Universitat Politècnica de València. https://doi.org/10.4995/CNRiegos.2015.1505OC

Usefulness of the ZIM-probe technology for detecting water stress in clementine and persimmon trees

Further improvement on irrigation management requires continuous plant water status monitoring. The non-invasive ZIM-probe measures the pressure (Pp) transfer function through a patch of an intact leaf, which is inversely correlated with the turgor pressure. Data are sent wireless in real-Time by telemetry to an internet server via a mobile phone network where it is available to be analyzed. In this work, the detection of water stress by measuring relative changes in turgor pressure with the ZIM-probe was evaluated in clementine and persimmon trees. Ten trees of both species were equipped with two ZIM-probes each located at the east side of the canopy. The ZIM-probes were used over several months during which half of the trees were subjected to two drought cycles. Concomitant measurements of stem water potential (ψs) were taken at midday in both orchards during the drought periods. Additionally, determinations of ψs and stomatal conductance (gs) were also performed during 1-2 days at hourly intervals in the clementine and persimmon orchards, respectively, to study the existing relationship of these classical indicators with the leaf turgor pressure. Results showed that diurnal Pp values increased in non-irrigated clementine trees when water restrictions were imposed. Persimmon drought-stressed trees, on the other hand, showed different Pp curve shapes (half and complete inverse curve) depending on the level of stress reached by the trees. There was a tight correlation between the hourly spot measurements of ψs and gs with the probe data. Overall, results show that the ZIM-probe enables the detection of drought stress in clementine and persimmon trees. Nevertheless, different approaches for calculating the water stress level must be used in each of these species due to the higher tendency of persimmon leaves to the inversed Pp curve phenomena

PROYECTO FIGARO: PLATAFORMA PARA LA GESTIÓN INTEGRAL Y EFICIENTE DEL RIEGO

[EN] Water and energy consumptions at plot level irrigation are issues of broad interest with an important environmental and economic impact. The solution to this double challenge requires the implementation of “precise irrigation”. In this work is shown the under developing software system FIGARO (Flexible and PrecIse IrriGation PlAtform to Improve FaRm Scale Water PrOductivity), which is part of a project co-funded by the European Commission under the 7th Framework Programme, in the scope of “Precision technologies to improve irrigation management and increase water productivity in major water-demanding crops in Europe” (KBBE.2012.1.1-03). The aim is to develop a tool to support decision-making for end users that let them perform irrigation scheduling with the aim of reducing water, energy and fertilizers consumptions, as well as yield crop is maximized. Implementation and validation of the FIGARO platform is being performed in several countries with different crops and weather conditions. First results in the year 2014, in citrus and grapevine crops, suggest increases in water productivity with savings close to 30% of water and 36% of energy at Water User Association level.[ES] El consumo de agua y energía en el ámbito del riego en parcela es una cuestión de amplio interés y consecuencias para el medio ambiente y la economía. La solución a este doble reto exige la implantación del denominado “riego de precisión”. En el presente trabajo se presenta la plataforma informática en desarrollo FIGARO (Flexible and PrecIse IrriGation PlAtform to Improve FaRm Scale Water PrOductivity), la cual forma parte de un proyecto financiado por la Unión Europea dentro del 7º Programa Marco, en el ámbito “Precision technologies to improve irrigation management and increase water productivity in major water-demanding crops in Europe” (KBBE.2012.1.1-03). El objetivo principal es desarrollar una herramienta de soporte a la toma de decisiones, que permita a los agricultores realizar programaciones de riego dirigidas a reducir el uso de agua, energía y fertilizantes, a la vez que se maximiza la productividad de las cosechas. La puesta en marcha y validación de la plataforma FIGARO se está llevado a cabo en diferentes países, con cultivos y condiciones climáticas diversas. Los primeros resultados de la campaña de 2014 en cultivos de cítrico y vid, sugieren incrementos de la productividad del agua con ahorros próximos al 30% de agua y del 36% de energía a nivel de comunidad de regantes.Este trabajo ha sido financiado por Unión Europea dentro del 7º Programa Marco, en el ámbito “Precision technologies to improve irrigation management and increase water productivity in major water-demanding crops in Europe” (KBBE.2012.1.1-03).Martínez Gimeno, M.; Martínez Alzamora, F.; Jiménez Bello, MÁ.; Royuela Tomás, Á.; Manzano Juarez, J.; Intrigliolo Molina, DS. (2015). PROYECTO FIGARO: PLATAFORMA PARA LA GESTIÓN INTEGRAL Y EFICIENTE DEL RIEGO. En XXXIII CONGRESO NACIONAL DE RIEGOS. Valencia 16-18 junio de 2015. Editorial Universitat Politècnica de València. https://doi.org/10.4995/CNRiegos.2015.1457OC

Distinct roles for PARP-1 and PARP-2 in c-Myc-driven B-cell lymphoma in mice

Dysregulation of the c-Myc oncogene occurs in a wide variety of hematologic malignancies, and its overexpression has been linked with aggressive tumor progression. Here, we show that poly (ADP-ribose) polymerase 1 (PARP-1) and PARP-2 exert opposing influences on progression of c-Myc-driven B-cell lymphoma. PARP-1 and PARP-2 catalyze the synthesis and transfer of ADP-ribose units onto amino acid residues of acceptor proteins in response to DNA strand breaks, playing a central role in the response to DNA damage. Accordingly, PARP inhibitors have emerged as promising new cancer therapeutics. However, the inhibitors currently available for clinical use are not able to discriminate between individual PARP proteins. We found that genetic deletion of PARP-2 prevents c-Myc-driven B-cell lymphoma, whereas PARP-1 deficiency accelerates lymphomagenesis in the E¿-Myc mouse model of aggressive B-cell lymphoma. Loss of PARP-2 aggravates replication stress in preleukemic E¿-Myc B cells, resulting in accumulation of DNA damage and concomitant cell death that restricts the c-Myc-driven expansion of B cells, thereby providing protection against B-cell lymphoma. In contrast, PARP-1 deficiency induces a proinflammatory response and an increase in regulatory T cells, likely contributing to immune escape of B-cell lymphoma, resulting in an acceleration of lymphomagenesis. These findings pinpoint specific functions for PARP-1 and PARP-2 in c-Myc-driven lymphomagenesis with antagonistic consequences that may help inform the design of new PARP-centered therapeutic strategies, with selective PARP-2 inhibition potentially representing a new therapeutic approach for the treatment of c-Myc-driven tumors.The J.Y. laboratory is funded by the Spanish Ministerio de Economía, Industria y Competitividad (grant SAF2017-83565-R), Spanish Ministerio de Ciencia e Innovación (grant PID2020-112526RB-I00), and Fundación Científica de la Asociación Española Contra el Cáncer (grant PROYEI6018YÉLA). Work in the J.E.S. laboratory is supported by a core grant to the Laboratory of Molecular Biology from the Medical Research Council (U105178808). The F.D. laboratory is supported by a Laboratory of Excellence grant (ANR-10-LABX-0034_Medalis) to Strasbourg University, Centre National de la Recherche Scientifique. The P.N. laboratory is supported by grants from the Spanish Ministry of Economy and Competitiveness/Instituto de Salud Carlos III–Fondo Europeo de Desarrollo Regional (FEDER; PI17/00199 and PI20/00625) and the Generalitat de Catalunya (2017-SGR-225). The P.M. laboratory acknowledges support from Centres de Recerca de Catalunya/Generalitat de Catalunya and Fundació Josep Carreras-Obra Social la Caixa for core support, the Spanish Ministry of Economy and Competitiveness (grant SAF-2019-108160-R), the Fundación Uno entre Cienmil, the Obra Social La Caixa (grant LCF/PR/HR19/52160011), and the German Josep Carreras Leukamie Stiftung. Work at the G.R. and P.M. laboratories are cofinanced by the European Regional Development Fund through the Interreg V-A Spain-France-Andorra Program (project PROTEOblood; grant EFA360/19). The O.F.-C. laboratory is funded by grants from the Spanish Ministry of Science, Innovation and Universities (RTI2018-102204-B-I00; cofinanced with European FEDER funds) and the European Research Council (ERC-617840). T.V.-H. was supported by a Marie Sklodowska Curie fellowship (GA792923). The A.B. laboratory is supported by the Spanish Ministry of Economy and Competitiveness (grant PID2019-104695RB-I00)

Dyslipidemias and stroke prevention: recommendations of the Study Group of Cerebrovascular Diseases of the Spanish Society of Neurology

Objetivo: Actualizar las recomendaciones de la Sociedad Española de Neurología para la prevención del ictus, tanto primaria como secundaria, en pacientes con dislipidemia. Desarrollo: Se ha realizado una revisión sistemática en Pubmed evaluando los principales aspectos relacionados con el manejo de las dislipidemias en la prevención primaria y secundaria del ictus, elaborándose una serie de recomendaciones relacionadas con los mismos. Conclusiones: En prevención primaria se recomienda determinar el riesgo vascular del paciente con el fin de definir los objetivos de LDLc. En prevención secundaria tras un ictus de origen aterotrombótico se recomienda un objetivo de LDLc < 55 mg/dl, mientras que en ictus isquémicos de origen no aterotrombótico, dado que su relación con dislipidemias es incierta, se establecerán los objetivos en función del grupo de riesgo vascular de cada paciente. Tanto en prevención primaria como secundaria las estatinas son los fármacos de primera elección, pudiendo asociarse ezetimiba y/o inhibidores de PCSK9 en aquellos casos que no alcancen los objetivos terapéuticosObjective We present an update of the Spanish Society of Neurology's recommendations for prevention of both primary and secondary stroke in patients with dyslipidaemia. Development We performed a systematic review to evaluate the main aspects of the management of dyslipidaemias in primary and secondary stroke prevention and establish a series of recommendations. Conclusions In primary prevention, the patient's vascular risk should be determined in order to define target values for low-density lipoprotein cholesterol. In secondary prevention after an atherothrombotic stroke, a target value < 55 mg/dL is recommended; in non-atherothombotic ischaemic strokes, given the unclear relationship with dyslipidaemia, target value should be established according to the vascular risk group of each patient. In both primary and secondary prevention, statins are the drugs of first choice, and ezetimibe and/or PCSK9 inhibitors may be added in patients not achieving the target valu

Spread of a SARS-CoV-2 variant through Europe in the summer of 2020.

Following its emergence in late 2019, the spread of SARS-CoV-21,2 has been tracked by phylogenetic analysis of viral genome sequences in unprecedented detail3–5. Although the virus spread globally in early 2020 before borders closed, intercontinental travel has since been greatly reduced. However, travel within Europe resumed in the summer of 2020. Here we report on a SARS-CoV-2 variant, 20E (EU1), that was identified in Spain in early summer 2020 and subsequently spread across Europe. We find no evidence that this variant has increased transmissibility, but instead demonstrate how rising incidence in Spain, resumption of travel, and lack of effective screening and containment may explain the variant’s success. Despite travel restrictions, we estimate that 20E (EU1) was introduced hundreds of times to European countries by summertime travellers, which is likely to have undermined local efforts to minimize infection with SARS-CoV-2. Our results illustrate how a variant can rapidly become dominant even in the absence of a substantial transmission advantage in favourable epidemiological settings. Genomic surveillance is critical for understanding how travel can affect transmission of SARS-CoV-2, and thus for informing future containment strategies as travel resumes. © 2021, The Author(s), under exclusive licence to Springer Nature Limited

CIBERER : Spanish national network for research on rare diseases: A highly productive collaborative initiative

Altres ajuts: Instituto de Salud Carlos III (ISCIII); Ministerio de Ciencia e Innovación.CIBER (Center for Biomedical Network Research; Centro de Investigación Biomédica En Red) is a public national consortium created in 2006 under the umbrella of the Spanish National Institute of Health Carlos III (ISCIII). This innovative research structure comprises 11 different specific areas dedicated to the main public health priorities in the National Health System. CIBERER, the thematic area of CIBER focused on rare diseases (RDs) currently consists of 75 research groups belonging to universities, research centers, and hospitals of the entire country. CIBERER's mission is to be a center prioritizing and favoring collaboration and cooperation between biomedical and clinical research groups, with special emphasis on the aspects of genetic, molecular, biochemical, and cellular research of RDs. This research is the basis for providing new tools for the diagnosis and therapy of low-prevalence diseases, in line with the International Rare Diseases Research Consortium (IRDiRC) objectives, thus favoring translational research between the scientific environment of the laboratory and the clinical setting of health centers. In this article, we intend to review CIBERER's 15-year journey and summarize the main results obtained in terms of internationalization, scientific production, contributions toward the discovery of new therapies and novel genes associated to diseases, cooperation with patients' associations and many other topics related to RD research

Epidemiological trends of HIV/HCV coinfection in Spain, 2015-2019

Altres ajuts: Spanish AIDS Research Network; European Funding for Regional Development (FEDER).Objectives: We assessed the prevalence of anti-hepatitis C virus (HCV) antibodies and active HCV infection (HCV-RNA-positive) in people living with HIV (PLWH) in Spain in 2019 and compared the results with those of four similar studies performed during 2015-2018. Methods: The study was performed in 41 centres. Sample size was estimated for an accuracy of 1%. Patients were selected by random sampling with proportional allocation. Results: The reference population comprised 41 973 PLWH, and the sample size was 1325. HCV serostatus was known in 1316 PLWH (99.3%), of whom 376 (28.6%) were HCV antibody (Ab)-positive (78.7% were prior injection drug users); 29 were HCV-RNA-positive (2.2%). Of the 29 HCV-RNA-positive PLWH, infection was chronic in 24, it was acute/recent in one, and it was of unknown duration in four. Cirrhosis was present in 71 (5.4%) PLWH overall, three (10.3%) HCV-RNA-positive patients and 68 (23.4%) of those who cleared HCV after anti-HCV therapy (p = 0.04). The prevalence of anti-HCV antibodies decreased steadily from 37.7% in 2015 to 28.6% in 2019 (p < 0.001); the prevalence of active HCV infection decreased from 22.1% in 2015 to 2.2% in 2019 (p < 0.001). Uptake of anti-HCV treatment increased from 53.9% in 2015 to 95.0% in 2019 (p < 0.001). Conclusions: In Spain, the prevalence of active HCV infection among PLWH at the end of 2019 was 2.2%, i.e. 90.0% lower than in 2015. Increased exposure to DAAs was probably the main reason for this sharp reduction. Despite the high coverage of treatment with direct-acting antiviral agents, HCV-related cirrhosis remains significant in this population

Deep-sequencing reveals broad subtype-specific HCV resistance mutations associated with treatment failure

A percentage of hepatitis C virus (HCV)-infected patients fail direct acting antiviral (DAA)-based treatment regimens, often because of drug resistance-associated substitutions (RAS). The aim of this study was to characterize the resistance profile of a large cohort of patients failing DAA-based treatments, and investigate the relationship between HCV subtype and failure, as an aid to optimizing management of these patients. A new, standardized HCV-RAS testing protocol based on deep sequencing was designed and applied to 220 previously subtyped samples from patients failing DAA treatment, collected in 39 Spanish hospitals. The majority had received DAA-based interferon (IFN) a-free regimens; 79% had failed sofosbuvir-containing therapy. Genomic regions encoding the nonstructural protein (NS) 3, NS5A, and NS5B (DAA target regions) were analyzed using subtype-specific primers. Viral subtype distribution was as follows: genotype (G) 1, 62.7%; G3a, 21.4%; G4d, 12.3%; G2, 1.8%; and mixed infections 1.8%. Overall, 88.6% of patients carried at least 1 RAS, and 19% carried RAS at frequencies below 20% in the mutant spectrum. There were no differences in RAS selection between treatments with and without ribavirin. Regardless of the treatment received, each HCV subtype showed specific types of RAS. Of note, no RAS were detected in the target proteins of 18.6% of patients failing treatment, and 30.4% of patients had RAS in proteins that were not targets of the inhibitors they received. HCV patients failing DAA therapy showed a high diversity of RAS. Ribavirin use did not influence the type or number of RAS at failure. The subtype-specific pattern of RAS emergence underscores the importance of accurate HCV subtyping. The frequency of “extra-target” RAS suggests the need for RAS screening in all three DAA target regions

CARB-ES-19 Multicenter Study of Carbapenemase-Producing Klebsiella pneumoniae and Escherichia coli From All Spanish Provinces Reveals Interregional Spread of High-Risk Clones Such as ST307/OXA-48 and ST512/KPC-3

ObjectivesCARB-ES-19 is a comprehensive, multicenter, nationwide study integrating whole-genome sequencing (WGS) in the surveillance of carbapenemase-producing K. pneumoniae (CP-Kpn) and E. coli (CP-Eco) to determine their incidence, geographical distribution, phylogeny, and resistance mechanisms in Spain.MethodsIn total, 71 hospitals, representing all 50 Spanish provinces, collected the first 10 isolates per hospital (February to May 2019); CPE isolates were first identified according to EUCAST (meropenem MIC &gt; 0.12 mg/L with immunochromatography, colorimetric tests, carbapenem inactivation, or carbapenem hydrolysis with MALDI-TOF). Prevalence and incidence were calculated according to population denominators. Antibiotic susceptibility testing was performed using the microdilution method (EUCAST). All 403 isolates collected were sequenced for high-resolution single-nucleotide polymorphism (SNP) typing, core genome multilocus sequence typing (cgMLST), and resistome analysis.ResultsIn total, 377 (93.5%) CP-Kpn and 26 (6.5%) CP-Eco isolates were collected from 62 (87.3%) hospitals in 46 (92%) provinces. CP-Kpn was more prevalent in the blood (5.8%, 50/853) than in the urine (1.4%, 201/14,464). The cumulative incidence for both CP-Kpn and CP-Eco was 0.05 per 100 admitted patients. The main carbapenemase genes identified in CP-Kpn were blaOXA–48 (263/377), blaKPC–3 (62/377), blaVIM–1 (28/377), and blaNDM–1 (12/377). All isolates were susceptible to at least two antibiotics. Interregional dissemination of eight high-risk CP-Kpn clones was detected, mainly ST307/OXA-48 (16.4%), ST11/OXA-48 (16.4%), and ST512-ST258/KPC (13.8%). ST512/KPC and ST15/OXA-48 were the most frequent bacteremia-causative clones. The average number of acquired resistance genes was higher in CP-Kpn (7.9) than in CP-Eco (5.5).ConclusionThis study serves as a first step toward WGS integration in the surveillance of carbapenemase-producing Enterobacterales in Spain. We detected important epidemiological changes, including increased CP-Kpn and CP-Eco prevalence and incidence compared to previous studies, wide interregional dissemination, and increased dissemination of high-risk clones, such as ST307/OXA-48 and ST512/KPC-3