El reto de la inclusión de los Objetivos de Desarrollo Sostenible en la formación inicial de profesores de secundaria: creación del MOOC curso cero sobre educación y ODS, inclusión en asignaturas y en trabajos fin de máster

Memoria ID-041. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2021-2022

Cualificación en los Objetivos establecidos en la Agenda 2030 de estudiantes y profesores en el Máster Universitario en Profesor de Educación Secundaria Obligatoria y Bachillerato, Formación Profesional y Enseñanza de Idiomas (MUPES)

Memoria ID2022-157 Ayudas de la Universidad de Salamanca para la innovación docente, curso 2022-2023

Innovaciones y mejoras en el proyecto tutoría entre compañeros. Curso 2015-2016

Memoria ID-0137. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2015-2016

A new kid in town: First case of an alien worm, Xironogiton victoriensis (Annelida: Clitellata) on a native European freshwater crayfish

Worldwide biodiversity is suffering a “biogeographical homogenization” due to biological invasions. The translocation of freshwater crayfish around the world for aquaculture and the pet trade is also spreading their symbionts, so that all of the association's members have the potential to form invasive populations. In Europe, the signal crayfish, Pacifastacus leniusculus, is often concomitant with the branchiobdellidan Xironogiton victoriensis and through repeated translocations their ranges are expanding. In this study, we report the first observation of X. victoriensis on a native European crayfish population of Austropotamobius pallipes. This work is an alert to the authorities and decision makers on the risks to native species when aquaculture projects introduce alien crayfish species and their symbionts into an area. Future studies are needed to evaluate the impact of this non-native ectosymbiont on native crayfish populations

The invasive alien red-eared slider turtle, Trachemys scripta, as a carrier of STEF-disease pathogens

The fungal pathogens Fusarium falciforme and Fusarium keratoplasticum are responsible for the sea turtle egg fusariosis (STEF) throughout main nesting areas of the world. In this study, we investigated whether eggs of the invasive alien red-eared slider turtle, Trachemys scripta, can carry these fungal pathogens. Using multilocus sequence typing of four nuclear DNA regions, we found that eggs of T. scripta naturally can carry these two Fusarium pathogenic species, as well as other Fusarium species belonging to the Fusarium solani species complex. Physiological studies on F. falciforme and F. keratoplasticum isolates revealed that their optimal growth temperature coincided with the pivotal temperature for T. scripta embryos, ca 29.5 ± 0.5 °C, providing an evidence of a potential advantageous biological property for host colonization and virulence. A host-pathogen interaction network analysis of species of the FSSC and their hosts confirmed that F. falciforme and F. keratoplasticum are generalist pathogens in a wide range of animal hosts of worldwide geographical distribution. Finally, we show that nesting areas of this invasive turtle T. scripta in the Mediterranean freshwater marshes can act as chronic reservoirs of these STEF pathogens, and this invasive species can act as a potential vector for the spread of STEF among wild native species and even to humans

Rainbow trout (Oncorhynchus mykiss) threaten Andean amphibians

[EN] Amphibian populations are declining due to a myriad of abiotic and biotic factors, including invasive species and pathogens. In temperate freshwater ecosystems, for example, amphibian populations are threatened by the predation of introduced salmonids. Salmonids not only directly predate upon amphibian eggs and larvae but may also transport deadly pathogens into freshwater systems. Though most research has focused on temperate systems, much less is known about the effects of introduced species in Neotropical streams. We conducted two experiments to investigate the impacts of rainbow trout (Oncorhynchus mykiss) in two Neotropical anurans. First, we assessed the effect of the rainbow trout on tadpole survivorship and morphology in Nymphargus grandisonae, a glassfrog species endemic of the Andes. Tadpoles of N. grandisonae were subjected to three treatments involving the absence of rainbow trout (control) and its presence with different types of chemical cues – kairomones (from rainbow trout) and cue alarms (from tadpole prey); the results show that the presence of rainbow trout affects the larval morphology of this glassfrog. In the second experiment, to test whether rainbow trout is a vector of the pathogenic freshwater mold Saprolegnia diclina (Oomycetes), eggs of Engystomops petersi were placed with infected and uninfected rainbow trout. There was a high mortality rate in the embryos of E. petersi exposed to trout infected with S. diclina. This represents the first evidence that rainbow trout may have a direct negative effect on Neotropical amphibian populations, and thus should be considered a threat. Management programs should be implemented to eradicate trout from Andean rivers, especially in areas with high number of endangered amphibians.[ES] Las poblaciones de anfibios están disminuyendo debido a una serie de factores, incluyendo las especies invasoras y patógenos. En los ecosistemas templados, por ejemplo, los anfibios están amenazados por salmónidos introducidos. Los salmónidos no solo depredan directamente los huevos y larvas, sino que también son vectores de patógenos en los ecosistemas acuáticos. A pesar de que existe una serie de investigaciones en zonas templadas, el efecto de las especies introducidas en sistemas neotropicales es prácticamente desconocido. En este trabajo estudiamos experimentalmente el efecto de la trucha arcoíris (Onchorhynchus mykiss) en dos especies de anuros neotropicales. Primero, determinamos el efecto de la trucha en la supervivencia y morfología de renacuajos de Nymphargus grandisonae, una rana de cristal endémica a los Andes. Los renacuajos fueron sometidos a tres tratamientos, los cuales combinan la ausencia de la trucha (control) y su presencia con diferentes señales químicas: cairomonas (de las truchas) y de alarma (producidas por los renacuajos depredados). Los resultados demuestran que la presencia de la trucha afecta la morfología de los renacuajos. En el segundo experimento, para determinar si la trucha actúa como vector del pátogeno Saprolegnia diclina (Oomycetes), los huevos del sapo Engystomops petersi fueron expuestos a la presencia truchas infectadas o no infectadas con S. diclina. El experimento muestra que la trucha actúa como vector de S. diclina, la cual produce una alta mortalidad en los huevos de E. petersi. Este es el primer estudio que claramente indica el efecto negativo de la trucha arcoíris en anfibios neotropicales. Recomendamos implementar programas de manejo dirigidos a erradicar a este pez introducido de los ríos andinos, especialmente en áreas con una alta presencia de especies amenazadas de anfibios.Peer Reviewe

Citas y apuntes corológicos de interés en ciperáceas ibéricas

We present chorological additions and corrections for four species of Iberian sedges of interest.Se presentan novedades y correcciones corológicas para cuatro especies de ciperáceas ibéricas de interés

Resistance to the crayfish plague, Aphanomyces astaci (Oomycota) in the endangered freshwater crayfish species, Austropotamobius pallipes

The pathogen Aphanomyces astaci Schikora 1906 is responsible for the decline of the native crayfish species of Europe, and their current endangered status. This pathogenic species is native to North America and only colonizes aquatic decapods. The North American crayfish species have a high resistance to this pathogen, while species from other regions are highly susceptible. However, recent field and laboratory observations indicate that there might exist some populations with resistance against this disease. The objective of this study was to test the susceptibility of 8 selected native European crayfish populations of Austropotamobius pallipes Lereboullet 1858 from the Pyrenees. We challenged them against the genome sequenced strain AP03 of A. astaci isolated from a North American red swamp crayfish, Procambarus clarkii Girard 1852, in the Garrotxa Natural Park, Girona. The results showed that there are significant differences (P<0,001) among populations, although most of them show high mortality rates after the zoospore challenge with A. astaci. However, one population from Girona exhibited a 100% survival during a four-month monitoring period under the experimental conditions tested. Histological analyses revealed a high immune reaction in tissues examined, i.e., encapsulation and melanization of hyphae, similar to that found in North American resistant crayfish species. These results represent the first observation of a native European crayfish population showing high resistance towards the most virulent genotype of this pathogen, i.e., genotype Pc. The identification of this population is of key importance for the management of these endangered species, and represents a crucial step forward towards the elucidation of the factors involved in the immune reaction against this devastating pathogen.This study has been funded by the European Commission in the framework of the LIFE Potamo Fauna project [LIFE12 NAT / ES / 001091] as a part of the group of actions for the conservation of the species A. pallipes. Additional funding was provided by Ministerio de Economía y Competitividad, Spain (CGL2016-80526-R), and Gobierno de Navarra

Resistance to the crayfish plague, Aphanomyces astaci (Oomycota) in the endangered freshwater crayfish species, Austropotamobius pallipes.

The pathogen Aphanomyces astaci Schikora 1906 is responsible for the decline of the native crayfish species of Europe, and their current endangered status. This pathogenic species is native to North America and only colonizes aquatic decapods. The North American crayfish species have a high resistance to this pathogen, while species from other regions are highly susceptible. However, recent field and laboratory observations indicate that there might exist some populations with resistance against this disease. The objective of this study was to test the susceptibility of 8 selected native European crayfish populations of Austropotamobius pallipes Lereboullet 1858 from the Pyrenees. We challenged them against the genome sequenced strain AP03 of A. astaci isolated from a North American red swamp crayfish, Procambarus clarkii Girard 1852, in the Garrotxa Natural Park, Girona. The results showed that there are significant differences (P<0,001) among populations, although most of them show high mortality rates after the zoospore challenge with A. astaci. However, one population from Girona exhibited a 100% survival during a four-month monitoring period under the experimental conditions tested. Histological analyses revealed a high immune reaction in tissues examined, i.e., encapsulation and melanization of hyphae, similar to that found in North American resistant crayfish species. These results represent the first observation of a native European crayfish population showing high resistance towards the most virulent genotype of this pathogen, i.e., genotype Pc. The identification of this population is of key importance for the management of these endangered species, and represents a crucial step forward towards the elucidation of the factors involved in the immune reaction against this devastating pathogen

Coexistence of Two Invasive Species, Procambarus clarkii and Aphanomyces astaci, in Brackish Waters of a Mediterranean Coastal Lagoon

Procambarus clarkii is a worldwide freshwater invasive crustacean from North America and was first introduced into Europe the 1970s. Along with P. clarkii, another invasive alien species was also spreading: Aphanomyces astaci. This pathogen is listed among the 100 World’s worst invasive species and involved in the European native crayfish decline. Although both species live in freshwater ecosystems, P. clarkii can withstand brackish waters and inhabit estuarine habitats. However, the presence of A. astaci associated to North American crayfishes has never been described in brackish waters. In this study, we have investigated the presence of A. astaci in a P. clarkii population of a Mediterranean coastal lagoon in the Albufera Natural Park, Valencia, Spain introduced in 1976. Our study confirmed the presence of this pathogen, and suggests that A. astaci has been spreading for more than four decades in the mentioned estuarine environment. Mitochondrial ribosomal rnnS and rnnL indicated that the isolated pathogen belongs to d1-haplotype (i.e., D-haplogroup) typically hosted by P. clarkii. The presence of this pathogen in a brackish environment may suggest a better adaptation than other A. astaci strains to adverse conditions, such as high salinity levels. This is a matter of concern for the conservation of European native freshwater crayfish and highlights once more the risk of introducing invasive alien crustaceans.e acknowledge support of the publication fee to the Frontiers Fee Support program and the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI). We want to thank the assistance CCEDCV-EL PALMAR (Conselleria d’Agricultura, Desenvolupament Rural, Emergència Climàtica i Transició Ecològica, Generatlitat Valenciana) for allowing the sampling and the performance of this research. We also want to thank the Master in Biodiversidad en Áreas Tropicales y su Conservación UIMP-CSIC, and María Martinez-Ríos and Gloria Casabella-Herrero from the RJB–CSIC for their technical assistance