Satisfacción del paciente desdentado total y evolución clínica tras el tratamiento rehabilitador sobre implantes

Tesis inédita de la Universidad Complutense de Madrid, Facultad de Odontología, Departamento de Estomatología III, leída el 12-07-2013Depto. de Especialidades Clínicas OdontológicasFac. de OdontologíaTRUEunpu

Supercritical fluid chromatography coupled to mass spectrometry: A valuable tool in food analysis

Producción CientíficaSupercritical fluid chromatography (SFC), although known for several decades, has undergone a growing interest in the last few years fueled by the introduction of modern instruments with improved robustness, and hyphenation to mass spectrometry (MS). This allows the analysis of trace compounds in complex samples with high selectivity, high sensitivity and in a short time, which has contributed to its increased use in the food analysis area. This work reviews the principal applications of SFC-MS in food analysis, highlighting the most important achievements

Efficacy of platelet-rich plasma applied to post-extraction retained lower third molar alveoli. A systematic review

Dental retentions have a high prevalence among the general population and their removal can involve multiple complications. The use of platelet rich plasma has been proposed in an attempt to avoid these complications, as it contains high growth factors and stimulates diverse biological functions that facilitate the healing of soft and hard tissues. Objectives: To evaluate the available scientific evidence related to the application of platelet-rich plasma in the post-extraction alveoli of a retained lower third molars. Material and Methods: A systematic review of published literature registered in the Medline, EMBASE, Cochrane and NIH databases. The following categories were included: human randomized clinical studies. Key search words were: platelet rich plasma; platelet rich plasma and oral surgery; platelet rich in growth factors and third molar. Results: Of 101 potentially valid articles, seven were selected, of which four were rejected as they failed to meet quality criteria. Three studies fulfilled all selection and quality criteria: Ogundipe et al.; Rutkowski et al.; Haraji et al. The studies all measured osteoblast activity by means of sintigraphy, and also registered pain, bleeding, inflammation, temperature, numbness as perceived by the patients, radiological bone density and the incidence of alveolar osteitis. Conclusions: Scientific evidence for the use of PRP in retained third molar surgery is poor. For this reason rando - mized clinical trials are needed before recommendations for the clinical application of PRP can be made

Study of different chiral columns for the enantiomeric separation of azoles using supercritical fluid chromatography

Producción CientíficaThe enantiomeric separation of antifungal compounds is an arduous task in pharmaceutical and biomedical fields due to the different properties that each diastereoisomer presents. The enantioseparation of a group of fungicides (sulconazole, bifonazole, triadimefon and triadimenol) using supercritical fluid chromatography was achieved in this work. For this goal, four different chiral columns based on polysaccharide derivatives, as well as the effect of different chromatographic parameters such as temperature, type and percentage of organic modifier (methanol, ethanol and isopropanol), were thoroughly investigated. The inversion of the elution order of enantiomers as a result of a change in the stationary phase or organic modifier was also evaluated by employing a circular dichroism detector. The best separation conditions, in terms of the enantioresolution and analysis time, were obtained with the Lux® Cellulose-2 column using isopropanol as the organic modifier

Evaluation of the anaesthetic properties and tolerance of 1:100,000 articaine versus 1:100,000 lidocaine. : a comparative study in surgery of the lower third molar

Objectives: To evaluate the anaesthetic properties and tolerance of articaine versus lidocaine at equal vasoconstrictor concentration. Study Design: A total of 96 male and female patients who underwent surgical treatment of the lower third molar participated. Patients were randomly assigned to articaine hydrochloride with epinephrine 1:100,000 and lidocaine hydrochloride with epinephrine 1:100,000. The variables analysed were latency period, duration of anaesthetic effect, tolerance and adverse reactions. Results: Both the latency period and the duration of anaesthetic effect were greater for articaine, although the differences were not statistically significant. Latency: mean difference of 2.70 ± 2.12 minutes (95%CI of -1.51 minutes - 6.92 minutes). Duration: mean difference of -33 minutes 5 seconds ± 31 minutes (95% CI -1 hour 35 minutes - 29 minutes). There were 4 adverse events that did not require the patients to be withdrawn from the study. Conclusions: The anaesthetics in this study have very similar properties for use in surgery and have demonstrated a good safety and tolerability profile. © Medicina Oral

Development and validation of a new method for the simultaneous determination of spinetoram J and L in honey from different botanical origins employing solid-phase extraction with a polymeric sorbent and liquid chromatography coupled to quadrupole time-of-flight mass spectrometry

Producción CientíficaThe objective of this study was to propose a novel method to determine residues of the bio-insecticide spinetoram, which is a mixture of two components (spinetoram J and L), in honey from multifloral, rosemary and heather botanical origins; liquid chromatography coupled to quadrupole time-of-flight mass spectrometry was the technique employed. An efficient sample treatment (recoveries between 82% and 95%) involving a solid phase extraction with a polymeric sorbent has been recommended, and no matrix effect was observed. Chromatographic analysis (4 min) was performed in reverse phase mode by using a fused-core column (Kinetex® EVO C18) with acetonitrile and ammonium formate as the mobile phase components, which was applied in isocratic elution mode. Method was validated according to the current European legislation. Not only was it selective, but it also displayed a wide linear range, good precision (relative standard deviation values lower than 9%) and sensitivity (low limits of detection (spinetoram J, 0.1–0.3 μg/kg; spinetoram L, 0.1–0.2 μg/kg) and quantification (spinetoram J, 0.3–1.2 μg/kg; spinetoram L, 0.4–0.7 μg/kg). Several honey samples were analyzed with this method and no spinetoram residues were found above the limits of detection.Este trabajo forma parte de los proyectos de investigación financiados por el Ministerio de Economía y Competitividad e INIA-FEDER (RTA2013-00042-C10-03 y 06

Quantification of growth factors by using a new system for obtaining platelet-rich plasma

Objective: To verify the performance of a new method for obtaining platelet-rich plasma, while avoiding contamination of the sample during its processing. Study Design: Twenty healthy patients were selected, from whom 21 ml of blood was extracted. We then pro-Design: extracted. We proceeded to study the platelets and growth factors in basal blood after centrifuging the sample by using a new closed system for obtaining platelet-rich plasma (PRP). Results: After centrifuging the blood sample, double the amount of platelets as that found in basal blood was obtained. Of the four growth factors analyzed, only the factor similar to insulin (IGF) contained the same concentration after the centrifuge process. The platelet-derived growth factor (PDGF) and the vascular growth factor (VGF) were multiplied by six with respect to the basal values and disproportionately increased the levels of the transforming growth factor ? (TGF-?). Conclusions: The new closed method for obtaining PRP, after avoiding contamination of the sample following its use, offers levels of platelet concentrate and growth factors necessary for regeneration. © Medicina Oral S. L

Trace analysis of flubendiamide in bee pollen using enhanced matrix removal-lipid sorbent clean-up and liquid chromatography-electrospray ionization mass spectrometry

Producción CientíficaIn this work, a new method has been proposed with the aim of determining flubendiamide, a recently commercialized insecticide, in bee pollen by using liquid chromatography coupled to electrospray ionization mass spectrometry. For this purpose, a novel sample treatment has been proposed that has proven efficient in terms of recovery (average analyte recoveries were between 90% and 102%) and absence of matrix effect, and one which is effective, fast and selective. This involved a solvent extraction using an acetonitrile and water mixture, and a clean-up stage where, in addition to freezing, an enhanced matrix removal-lipid sorbent was successfully used for the first time with this matrix and analyte. The chromatographic conditions were also optimized, by selecting a C18 based column (Gemini® C18) and acetic acid (1 mM) in water and methanol as mobile phase components, allowing elution of flubendiamide in<4 min, with a total analysis time of 14 min. Validation was carried out, with the result that all the parameters studied complied with existing European legislation. It should be noted that the sensitivity of the method was excellent, with a quantification limit (4 μg/kg) well below the maximum residue level established for this insecticide in bee products (50 μg/kg). Finally, several bee pollen samples were analyzed, and flubendiamide residues were not found in any of the cases.Este trabajo forma parte de los proyectos de investigación financiados por el Ministerio de Economía y Competitividad e INIA-FEDER (RTA2013-00042-C10-03 y 06)

Maximizing antenna array aperture efficiency for footprint patterns

Despite playing a central role in antenna design, aperture efficiency is often disregarded. Consequently, the present study shows that maximizing the aperture efficiency reduces the required number of radiating elements, which leads to cheaper antennas with more directivity. For this, it is considered that the boundary of the antenna aperture has to be inversely proportional to the half-power beamwidth of the desired footprint for each ¿-cut. As an example of application, it has been considered the rectangular footprint, for which a mathematical expression was deduced to calculate the aperture efficiency in terms of the beamwidth, synthesizing a rectangular footprint of a 2:1 aspect ratio by starting from a pure real flat-topped beam pattern. In addition, a more realistic pattern was studied, the asymmetric coverage defined by the European Telecommunications Satellite Organization, including the numerical computation of the contour of the resulting antenna and its aperture efficiency.Postprint (published version

Improvement of Edge Brightening by Means of Q Factor Minimization in Circular Antenna Apertures: High Efficient Taylor-Like Patterns

Implications and improvements of edge brightening effects led by Q factor minimization restricted to keep the same level of directivity for high efficiency continuous circular aperture distributions are here reported. In this manner, an optimization strategy for a minimum Q value-keeping the same level of efficiency and restricting the maximum sidelobe level (SLL)- is envisaged. As application of the method, a design procedure devoted to reduce the Q factor of the antenna aperture distributions while keeping a high level of efficiency is outlined. Then, these optimal Taylor distributions are used as initial point to develop an optimization strategy. This procedure is devoted to search Taylor-like distributions which offer a good compromise between low Q factor and high efficiency values with potentials for the antenna design scenario, based on a decrease in edge brightening effects led by the minimization of the aforementioned Q ratioS