Hepatic capillariasis in two captive Callithrix penicillata

O presente trabalho relata a ocorrência de dois casos de capilariose hepática em primatas não-humanos da espécie Callithrix penicilatta mantidos em cativeiro. O caso 1 era uma fêmea jovem, que exibiu fígado de cor pálida, suavizada devido a autólise e algumas áreas múltiplas visivelmente esbranquiçadas. O caso 2 foi registrado em um macho adulto, de idade desconhecida, com fígado levemente pálido e acastanhado. A análise histológica das amostras revelou extensa fibrose intersticial com áreas de hemossiderose, exemplares de Capillaria hepatica adultos e numerosos ovos morfologicamente caracterizados pela sua forma elipsóide, não-embrionárias, com tampões bipolares que se sobressaem da casca, que é espessa, birrefringente e com uma camada porosa, dando uma aparência estriada ao ovo. Algumas formações císticas continham ovos do nematóide cercados por numerosas células gigantes do tipo corpo estranho. A literatura consultada possui poucas referências acerca da presença de Capillaria hepatica em primatas não-humanos. No entanto, infecções semelhantes são bem estudadas em roedores domésticos.This study aimed to describe the occurrence of hepatic capillariasis in two non-human primates of the species Callithrix penicillata that were kept in captivity. Case 1 was in a young female, whose liver presented pale coloring, softening due to autolysis and several noticeably whitish areas. Case 2 was in a male of unknown age, whose liver was slightly pale and brownish. Histological analysis on the samples revealed extensive interstitial fibrosis with hemosiderotic areas. Adult nematodes of Capillaria hepatica and numerous eggs, morphologically characterized by their non-embryonic ellipsoid shape, with bipolar plugs that did not protrude from the shell, thick and birefringent, with a porous layer, thus giving a striated appearance to the eggs. Some cystic formations presented nematode eggs surrounded by numerous foreign-body giant cells. The literature consulted made little reference to reports of Capillaria hepatica in non-human primates. However, similar infections have been extensively studied in domestic rodents.

Infiltrative Rectal Adenocarcinoma in a Dog

Background: Intestinal neoplasms are uncommon in dogs and adenocarcinoma is the main histological type found. This neoplasm presents slow growth and high capacity of causing metastasis. Histologically speaking, neoplasm cells can present solid, tubular, papillary arrangement and note amorphous extra-cellular material. Clinically observed tenesmus, diarrhea, dyskinesia, hematochezia, mane, protrusion of the anus, weight loss, anorexia. The occurrence and clinicopathological aspects of tumors in dogs’ gastrointestinal tract, the rectal segment, remains poorly understood. Accordingly, the aim of the present study is to report a case on infiltrative rectal adenocarcinoma diagnosed in a dog.Case: A 7-year-old male dog representative of the Fila Brasileiro breed was presented to the Veterinary Hospital of University Federal Rural of Amazonia, with history of hyperthermia, anorexia, apathy and tenesmus. Imaging examinations depicted prostatomegaly. Exploratory laparotomy was performed and showed the thickening and hardening of the rectum segment. The animal was subjected to euthanasia. Necroscopy showed increased rectal perimeter; the mucosa in its opening presented atypical cerebroid aspect and irregular surface, and areas dark red. The rectal segment depicted a thick wall of  white color, irregular limits covering the muscular and adjacent sub-mucosa. The peri-rectal adipose tissue presented poor delimitation with the rectum, multiple greyish and reddish areas. Increased prostate and iliac lymph, and multi node of regular limits in the lungs. The histology of the rectal tissue depicted epithelium with differentiated neoformation, composed of atypical cells; nuclear anisocytosis, anisocariasis and hyperchromasia placed in small islands, cords or tubular formation. Neoplasm growth was unorganized and of infiltrative character. Some areas presented mucosal pattern cells with Signal Ring morphologic. Multiple rectal blood vessels, regional lymph nodes and lungs had neoplasm growth similar to that observed in the intestine. Mucosa also presented ulceration areas and lymphoplasmacytic infiltrate. There was fibroplasia, lymphoplasmacytic points and bleeding in the serous, as well as in the peri-rectal fat tissue. The immunohistochemical technique showed immunostaining in cytokeratin and vimentin antibodies, and in marked epithelial cells and tumor stroma markings, respectively.Discussion: The intestinal tumor diagnosis in dogs is found by associating history, clinical signs, radiographic, ultrasound findings and necropsty. Only one data about the occurrence of rectal adenocarcinoma in Fila Brasileiro specimens. With regards to sex, results were similar to those record, whose males presented higher prevalence of primary rectal tumors. The macroscopic characteristic is consistent with infiltrative neoplasms; thickening was related to the presence of the tumor. The histopathological findings evidenced growing infiltrative neoplasm formed by atypical cells of tubular arrangement. Microscopy featured a chronic ulcerative colitis frame, such alteration represents one of the main risk factors for colon rectal cancer in humans. Epithelial histogenesis was confirmed through immunohistochemical results that have revealed co-expression of the cytokine epithelial marker in most tumor cells. The vimentin mesenchymal marker in the neoplasm stroma was positive, fact that can be explained by occasional immune-reaction in the anti-bodies (cytokine and vimentin) and in non-differentiated carcinomas. The prognostic was negative in the current report. Such outcome was attributed to the infiltrative character observed in the trans-operative period. Necropsy, as well as the histopathological and immunohistochemical exams, confirmed the infiltrative rectal adenocarcinoma in the dog

Cysticercosis in free-ranging agoutis (Dasyprocta leporina) in the Eastern Brazilian Amazon

Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Patologia. São Paulo, SP, Brasil / Universidade Paulista. Instituto de Ciências da Saúde. Campinas, SP, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Centro Nacional de Primatas. Ananindeua, PA, Brasil.Universidade de São Paulo. Instituto de Medicina Tropical. Departamento de Gastroenterologia. São Paulo, SP, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.The study describes the occurrence of cysticercosis in liver of 22 wild agoutis (Dasyprocta leporina) in the Brazilian Amazon. The phylogenetic analysis and microscopic characteristics of metacestodes in liver tissue sections, associated with the geographic distribution of the intermediate hosts indicated that a possibly novel Taenia sp. metacestode caused the parasitism. Additionally, two cases of hepatic co-infection by Taenia sp., Calodium sp. and Echinococcus oligarthra were also observed among the analyzed animals. The results point to the need for a better understanding of hepatotropic parasites among wild rodents in the Brazilian Amazon

Tuberculosis Caused by Mycobacterium tuberculosis Complex in a Captive Tapir (Tapirus terrestris)

Background: Tuberculosis is an infectious disease caused by the Mycobacterium tuberculosis complex has already been identified in a great number of wild species. Captive animals may have a potential source for zoonoses, because is related to factors such as cohabitation, direct contact with the public, presence of biological vectors, nutritional deficiencies, absence of sanitary barriers, precautionary hygienic measures and sanitary vigilance. In Brazil, there is little information on tuberculosis in captivity animals, and little attention is given to the risks of disease transmission from humans. The aim of this study was to report the first diagnosed case of tuberculosis Tapirus terrestris in Brazilian Amazon region.Case: One Tapirus terrestris was kept by local zoobotanic foundation in city of Marabá, Southeast of Pará state, Brazilian Amazon, and became ill. Physical examination revealed cough, sneezing, nasal outflow, dyspnea, hyperthermia and lethargy, leading to death. Necropsy demonstrated severe pulmonary alterations: thickening of the inter-alveolar septa, alveolar emphysema, and miliary nodules with dimensions up to 5 mm, which were yellowish-white, caseous, and sometimes calcified. Additionally, large areas of caseous compaction of the parenchyma, characteristic of caseous tuberculosis. Histopathological analysis revealed a process characteristic of mycobacterial infection, with alveoli filled with caseous exudate and thickened septa and fibrocytes, in addition to recently formed tubercles, some with caseous necrosis, calcifications and Langhans cells. In the Ziehl-Neelsen staining, alcohol-acid resistant bacilli were observed in the mesenteric lymph nodes. No mycobacterial growth was observed in Lowenstein-Jensen culture medium. A nested PCR followed by a sequencing assay targeting the hsp65 gene and M. tuberculosis complex member was detected. Water and M. tuberculosis H37Rv were used as negative and positive controls, respectively.Discussion: This article reports the first case of tuberculosis in T. terrestris in Amazonia. The case of infection was caused by a complex of Mycobacterium tuberculosis, as well as reports of other studies described in wild animals. This record presented clinical and pathological similarity with the cases of a tapir and coati at the zoo of Curitiba, and non-human primates in bioteries in Belém. The disease was reported, also, in a T. indicus female in Bangkok, Thailand. In another study with two tapirs there was positivity of tuberculin test result in a Swedish zoo. In necropsy findings of tuberculosis in a Malayan tapir showed multiple white, caseous nodules spread throughout the lungs. Studies have shown that lesions can be in various organs, among them the mediastinal and mesenteric lymph nodes, liver, spleen, kidneys, ovaries, serosa, diaphragm, intestines and uterus. For exemple in a case of disseminated tuberculosis caused by M. tuberculosis in a Malayan tapir were described multiple granulomes, some coalescent, characterized by necrotic caseous centers, with some Langhans giant cells and a discrete fibrotic zone. In the present work, the results of the anatomopathological and molecular assays confirmed the clinical suspicion of respiratory infection and established the conclusive diagnosis of pulmonary tuberculosis caused by M. tuberculosis complex member. It is worth noting that tuberculosis is an important zoonotic disease affecting survival of the species, posing an extinction threat and also a public health concern

Adenocarcinoma papilífero oral em jiboia (Boa constritor) mantida em cativeiro

Universidade Federal Rural da Amazônia. Belém, PA, BrazilUniversidade Federal Rural da Amazônia. Programa de Pós-Graduação em Saúde e Reprodução Animal na Amazônia. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilMuseu Emílio Goeldi. Belém, PA, BrazilSem AfiliaçãoInformation on neoplasms in animals has increased over time, and these studies have helped in the management of reptiles that present tumors. There are similar incidences of neoplasms between homeothermic and ectothermic animals. Furthermore, there are usually more than one type of tumor present. The treatment of wild animals afflicted with cancer usually happens late, contributing to their low life expectancy. Thus, the present work aimed to describe an infrequent case of oral tumor in Boa constrictor. The tumor was observed in an adult female animal, raised in an exhibition area of the Zoo and Botanical Park of the Emílio Goeldi Museum, located in Belém, State of Pará, Brazil. Macroscopically, the mass presented morphologically with an irregular, multilobulated surface, color that varied from white to grey, hemorrhagic areas and its extension was 3.9 x 2.3 cm. The neoplasm was surgically removed, and the histopathological evaluation revealed an adenocarcinoma, with a papillary-like development pattern and a moderate degree of differentiation. The animal died three months after diagnosis due to starvation. The necropsy showed that there was tumor recrudescence and no metastases. Given the impossibility of surgical removal with a greater margin of safety, and adjuvant therapies, this condition favoured the resurgence of the neoplasm. This compromised the animal’s ability to feed and consequently lead to death. Malignant neoplasms in reptiles may have an unfavourable clinical evolution for the maintenance of life, requiring specific therapeutic care such as chemotherapy. Scientific contributions on tumors in these animals are essential for the medical treatment of wild animals, and the conservation of wild specie

Leptospirosis, bovine viral diarrhea and infectious bovine rhinotracheitis: prevalence in Colombian cattle and buffaloes

One of the limiting factors of productive efficiency in cattle and buffalo herds is related to the high prevalence of infectious diseases which affect reproduction. The aim of this study was to determine the prevalence of anti-Leptospira antibodies for bovine viral diarrhea virus (BVDV) and bovine herpesvirus type 1 (BoHV-1) in bovine and buffalo herds in Colombia. Blood serum samples were collected from 1100 buffaloes and 1000 cattle. The ELISA technique was used to detect antibodies against BVDV and BoHV-1, and the microscopic agglutination technique to detect anti-Leptospira antibodies. The prevalence of anti-Leptospira antibodies and of BVDV and BoHV-1 in bovine samples was observed in 16, 39.7, and 65% of animals, respectively, while the positivity in samples for the same antibodies in buffalos was detected in 18.7, 27.5 and 51.5%, respectively. Exposure of cattle and buffaloes to BoHV-1 was positively associated with age, higher prevalence rates were observed in older ages. Seropositivity of cattle for BVDV and BoHV-1 was higher in male animals. Activities such as embryo transfer, milking, and needle reuses, as well as the presence of cats and rodents are factors which favor positivity of the herd for BVDV and BoHV-1.Incluye referencias bibliográficas

Estudo de arbovírus em Philander opossum, Didelphis marsupialis e Nectomys rattus capturados em fragmentos florestais no município de Belém, Pará

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Federal Rural da Amazônia. Laboratório de Patologia Animal. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Federal Rural da Amazônia. Laboratório de Patologia Animal. Belém, PA, Brasil.Universidade Federal Rural da Amazônia. Laboratório de Patologia Animal. Belém, PA, Brasil.Universidade Federal do Pará -Campus Castanhal. Castanhal, PA, Brasil.Universidade Federal Rural da Amazônia. Laboratório de Patologia Animal. Belém, PA, Brasil.Arboviruses are viruses that maintain their life cycle in the wild and are transmitted to vertebrate hosts by hematophagous diptera. They are zoonotic and can establish an enzootic cycle in the urban areas; in humans, the infection can manifest from being encephalitogenic to hemorrhagic. This study aimed to report the occurrence of arboviruses in mammals of the order Didelphimorphia and Rodentia, captured from the Amazon. Serum samples were subjected to hemagglutination inhibition test using a viral panel of 19 species of arboviruses that are known to occur in the Amazon. Altogether, 14 wild mammals, 12 of Philander opossum, 1 of Didelphis marsupialis, and 1 of Nectomys rattus were captured. Eight of these were reported to be seropositive for arboviruses (57.14%) with monotypic seroprevalence for the Eastern Equine Encephalitis Virus (n=1), the Ilheus Virus (n=2), and the Catu virus (n=4); 4 heterotypic responses were observed for Flavivirus and Orthobunyavirus. In conclusion, several arbovirus species are in active circulation and maintenance, exhibiting enzootic characteristics in the wild mammals of the Amazon region; these animals prove to be potential hosts in the transmission of diseases to humans

Capilariose hepática em dois Callithrix penicillata de cativeiro

Universidade Federal Rural da Amazônia. Instituto da Saúde e Produção Animal. Laboratório de Patologia Animal. Belém, PA, Brazil.Universidade Federal Rural da Amazônia. Instituto da Saúde e Produção Animal. Laboratório de Parasitologia Veterinária. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Centro Nacional de Primatas. Ananindeua, PA, Brasil.Universidade Federal Rural da Amazônia. Instituto da Saúde e Produção Animal. Laboratório de Patologia Animal. Belém, PA, Brazil.Universidade Federal Rural da Amazônia. Instituto da Saúde e Produção Animal. Laboratório de Patologia Animal. Belém, PA, Brazil.This study aimed to describe the occurrence of hepatic capillariasis in two non-human primates of the species Callithrix penicillata that were kept in captivity. Case 1 was in a young female, whose liver presented pale coloring, softening due to autolysis and several noticeably whitish areas. Case 2 was in a male of unknown age, whose liver was slightly pale and brownish. Histological analysis on the samples revealed extensive interstitial fibrosis with hemosiderotic areas. Adult nematodes of Capillaria hepatica and numerous eggs, morphologically characterized by their non-embryonic ellipsoid shape, with bipolar plugs that did not protrude from the shell, thick and birefringent, with a porous layer, thus giving a striated appearance to the eggs. Some cystic formations presented nematode eggs surrounded by numerous foreign-body giant cells. The literature consulted made little reference to reports of Capillaria hepatica in non-human primates. However, similar infections have been extensively studied in domestic rodents

Análise molecular de Bartonella spp. em tecido hepático de morcegos do bioma Mata Atlântica, Brasil

Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade de São Paulo. School of Veterinary Medicine and Animal Science. Department of Pathology. São Paulo, SP, Brazil / Universidade Paulista. Institute of Health Sciences. Campinas, SP, Brazil.Instituto Pasteur. Health Department, Disease Control Coordination. São Paulo, SP, Brazil.Instituto Pasteur. Health Department, Disease Control Coordination. São Paulo, SP, Brazil.University of Sao Paulo. School of Veterinary Medicine and Animal Science. Department of Pathology. São Paulo, SP, Brazil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Laboratório de Biologia Molecular. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Federal Rural da Amazônia. Department of Pathology Animal. Belém, PA, Brazil.The genus Bartonella comprises gram-negative bacilli that possess tropism for erythrocytes and endothelial cells in animals of the orders Rodentia, Lagomorpha, Carnivora, Artiodactyla, Eulipotyphla, and Chiroptera. Bacterial infection may be associated with lymphadenitis, endocarditis, bacillary angiomatosis, and peliosis hepatis. Thirteen species of Bartonella are recognized as zoonotic and bats are considered to be their potential reservoirs. This study aimed to analyze the occurrence of Bartonella spp. in livers of neotropical bats belonging to the families Molossidae, Phyllostomidae, and Vespertilionidae, classified into 21 genera from the Atlantic Forest biome in São Paulo. A total of 341 (n = 341) chiropterans samples were tested for the presence of citrate synthase (gltA) gene of Bartonella by partial amplification using polymerase chain reaction (PCR). Samples of two bats (0.6%) of the species Glossophaga soricina from the municipality of São Roque were tested positive for gltA gene. Phylogenetic analysis indicated that sequences clustered in a clade that was close to the Bartonella sp. detected in G. soricina, which was collected from the Cerrado biome in Tocantins, Brazil. Despite a low prevalence of the detected infection, results indicated that neotropical bats from the Atlantic Forest were potential hosts of Bartonella spp., which might be related to the maintenance of a wild enzootic cycle of the bacterium. Additional studies, particularly on bats of genus Glossophaga, are required to elucidate the dynamics of intraspecific relationships between etiologic agent-vector-hosts