587 research outputs found
Pretreatments applied to microalgae residues to enhance anaerobic digestion
ABSTRACT: Biomass of microalga Chlorella protothecoides, grown under autotrophic and heterotrophic
conditions and subjected to pretreatments, were energetically valorized through anaerobic digestion process according to the substrates: autotrophic algae (A), heterotrophic algae (H), heterotrophic algae extracted (HE), autoclave pretreated heterotrophic algae (HPA), enzyme pretreated heterotrophic algae (HPE), ultrasound pretreated heterotrophic algae (HPU), and inoculum (I). Despite the application of pretreatments, the highest methane production was obtained in the algae extracted digestion with 172 mL CH4, against 153, 126 and 142 mL obtained in HPA, HPE and HPU, respectively. The COD removal capacity was higher in the HPA sample while the TS and VS removal reached higher values in the autotrophic alga.N/
Hybrid structures made of polyurethane/graphene nanocomposite foams embedded within aluminum opencell foam
This paper focuses on the development of hybrid structures containing two different classes of porous materials, nanocomposite foams made of polyurethane combined with graphene-based materials, and aluminum open-cell foams (Al-OC). Prior to the hybrid structures preparation, the nanocomposite foam formulation was optimized. The optimization consisted of studying the effect of the addition of graphene oxide (GO) and graphene nanoplatelets (GNPs) at different loadings (1.0, 2.5 and 5.0 wt%) during the polyurethane foam (PUF) formation, and their effect on the final nanocomposite properties. Globally, the results showed enhanced mechanical, acoustic and fire-retardant properties of the PUF nanocomposites when compared with pristine PUF. In a later step, the hybrid structure was prepared by embedding the Al-OC foam with the optimized nanocomposite formulation (prepared with 2.5 wt% of GNPs (PUF/GNPs2.5)). The process of filling the pores of the Al-OC was successfully achieved, with the resulting hybrid structure retaining low thermal conductivity values, around 0.038 W∙m−1∙K−1, and presenting an improved sound absorption coefficient, especially for mid to high frequencies, with respect to the individual foams. Furthermore, the new hybrid structure also displayed better mechanical properties (the stress corresponding to 10% of deformation was improved in more than 10 and 1.3 times comparatively to PUF/GNPs2.5 and Al-OC, respectively).publishe
High affinity of 3D spongin scaffold towards Hg(II) in real waters
This study focuses on the ability of commercial natural bath sponges, which are made from the skeletons of marine sponges, to sorb Hg from natural waters. The main component of these bath sponges is spongin, which is a protein-based material, closely related to collagen, offering a plenitude of reactive sites from the great variety of amino acids in the protein chains, where the Hg ions can sorb. For a dose of 40 mg L-1 and initial concentration of 50 μg L-1 of Hg(II), marine spongin (MS) removed ~90% of Hg from 3 water matrixes (ultrapure, bottled, and seawater), corresponding to a residual concentration of ~5 μg L-1, which tends to the recommend value for drinking water of 1 μg L-1. This value was maintained even by increasing the MS dosage, suggesting the existence of a gradient concentration threshold below which the Hg sorption mechanism halts. Kinetic modelling showed that the Pseudo Second-Order equation was the best fit for all the water matrixes, which indicates that the sorption mechanism relies most probably on chemical interactions between the functional groups of spongin and the Hg ions. This material can also be regenerated in HNO3 and reused for Hg sorption, with marginal losses in efficiency, at least for 3 consecutive cycles.publishe
A multifactorial approach to untangle graphene oxide (GO) nanosheets effects on plants: plant growth-promoting bacteria inoculation, bacterial survival, and drought
Drought is a limiting factor for agricultural productivity. Climate change threatens to expand the areas of the globe subjected to drought, as well as to increase the severity and duration of water shortage. Plant growth-promoting bacteria (PGPB) are widely studied and applied as biostimulants to increase plant production and to enhance tolerance to abiotic and biotic constraints. Besides PGPB, studies on the potential of nanoparticles to be used as biostimulants are also thriving. However, many studies report toxicity of tested nanoparticles in bacteria and plants in laboratory conditions, but few studies have reported effects of nanoparticles towards bacterial cells and communities in the soil. The combined application of nanoparticles and PGPB as biostimulant formulations are poorly explored and it is important to unravel the potentialities of their combined application as a way to potentiate food production. In this study, Rhizobium sp. E20-8 and graphene oxide (GO) nanosheets were applied on container-grown maize seedlings in watered and drought conditions. Bacterial survival, seedling growth (dry weight), and biochemical endpoints (photosynthetic pigments, soluble and insoluble carbohydrates, proline, lipid peroxidation, protein, electron transport system, and superoxide dismutase) were evaluated. Results showed that the simultaneous exposure to GO and Rhizobium sp. E20-8 was able to alleviate the stress induced by drought on maize seedlings through osmotic and antioxidant protection by GO and mitigation of GO effects on the plant's biochemistry by Rhizobium sp. E20-8. These results constitute a new lead on the development of biostimulant formulations to improve plant performance and increase food production in water-limited conditions.publishe
Electrospraying of primary chondrocytes for cartilage repair
Electrospun scaffolds have long been used for cartilage repair, due to the topographic similarity between the electrospun fibers and the collagen fibers of the extracellular matrix (ECM) in the native cartilage. Still, while their nanotophography can be beneficial for the cell proliferative and spreading behavior, it greatly reduces the inter-fiber pore size, hindering cell migration and relegating tissue formation to the surface of the scaffold [1]. A possible solution for this structura l limitation would be the direct incorporation of cells into the fibers during electrospinning of the fibrous scaffold, overcoming the challenges of cell infiltration into small pore sizes by literally surrounding cells with the fiber matrix as it is produced [1]. This can be achieved using cell electrospraying, a concept first introduced in 2005 by Jayasinghe, enables the deposition of living
cells onto specific targets by exposing the cell suspension to an external high intensity electric field [2]. Cell exposure to the electric field, as well as the shear stress of passing through the cell electrospraying apparatus may affect cell viability and function, so several types of cells have been electrosprayed, and no significant influence was observed on a genetic, genomic and physiological level [4]. In fact, our previous work has demonstrated this inertness from a chondrocyte cell line (C28-I2)
[5]. Still, these immortalized cells are genetically modified, and might not not accurately replicate the physiological conditions. Primary chondrocytes possess little proliferative ability, showing considerable dedifferentiation from a chondrocyte-like to a more
fibroblast-like phenotype over time, particularly if growth factors are not used [5]. In this regard, electrospraying experiments were performed with primary chondrocytes to assess the process influence on chondrocyte viability. After 24 hour-incubation, chondrocyte metabolic activity was measured, and these electrosprayed (E) cells were then slip and cultured in well plates and in threedimensional anisotropic fibrous/porous scaffolds under static and perfused conditions. Non-electrosprayed (NE) cells were considered for comparison. The obtained results confirmed that the behaviour of primary chondrocytes upon electric field exposure was significantly different from that obtained for the chondrocyte cell line, which can be attributed to the lower recovery ability of these cells. Nonetheless, an increasing proliferation rate was observed over time. The proliferation performance of NE and E primary chondrocytes on 3D environment followed a similar trend, with E primary chondrocytes possessing a significantly lower viability than the NE primary chondrocytes. The application of perfused conditions to the E chondrocyte-seeded scaffolds greatly increased the chondrocyte viability to values similar to the ones obtained for NE chondrocyte-seeded scaffolds. Even though the electrosprayed primary chondrocytes suffered a substantial proliferative delay,
they were able to recover, particularly under perfused conditions, suggesting that these conditions should be implemented after the electrospraying process, so that this technology might become an effective approach to uniformly incorporate primary chondrocytes into electrospun scaffolds.publishe
Green graphene-chitosan sorbent materials for mercury water remediation
The development of new graphene-based nanocomposites able to provide synergistic effects for the adsorption of toxic heavy metals in realistic conditions (environment) is of higher demand for future applications. This work explores the preparation of a green nanocomposite based on the self-assembly of graphene oxide (GO) with chitosan (CH) for the remediation of Hg(II) in different water matrices, including ultrapure and natural waters (tap water, river water, and seawater). Starting at a concentration of 50 μg L-1, the results showed that GO-CH nanocomposite has an excellent adsorption capacity of Hg (II) using very small doses (10 mg L-1) in ultrapure water with a removal percentage (% R) of 97 % R after only two hours of contact time. In the case of tap water, the % R was 81.4% after four hours of contact time. In the case of river and seawater, the GO-CH nanocomposite showed a limited performance due the high complexity of the water matrices, leading to a residual removal of Hg(II). The obtained removal of Hg(II) at equilibrium in river and seawater for GO-CH was 13% R and 7% R, respectively. Our studies conducted with different mimicked sea waters revealed that the removal of mercury is not affected by the presence of NO3- and Na+ (>90% R of Hg(II)); however, in the presence of Cl-, the mercury removal was virtually nonexistent (1% R of Hg(II)), most likely because of the formation of very stable chloro-complexes of Hg(II) with less affinity towards GO-CH.publishe
Fabrication of electrospun scaffolds with cell laden hydrogel for cartilage tissue engineering
Tissue engineering strategies create artificial substitutes for the regeneration of damaged tissues, beginning with the fabrication of scaffolds moving then to cell incorporation onto those scaffolds and subsequent tissue growth in vitro. Cell seeding techniques, unfortunately, are usually ineffective to develop scaffolds with homogenous cell distribution, resulting in non-functional tissue formation [1]. With electrospun scaffolds, cell incorporation becomes even more challenging. Electrospun scaffolds are a very tightly packed layer of fibers with small pores, that makes difficult the migration of cells onto the scaffolds, as well as, the diffusion of nutrients and wastes. To overcome this drawback, the direct incorporation of cells, using electrospraying technique, onto the scaffolds during the electrospinning process has been reported. Cell electrospraying is a jet-based technique that allows the spray of living cells onto the materials by applying an electric charge in a cellular suspension [2]. Several studies have proved that cells can survive and proliferate after electrospraying process [3], [4]. Still, previous work has shown that while uniformly distributed cell-laden scaffolds can be fabricated using this technique, some issues remain. Cell desiccation on top of the fibers due to longer duration of the experiment and inadequate cell environment – low temperature and CO2 concentration – and solvent toxicity are the main limitations for the optimal efficiency of cell electrospray process onto electrospun fibers. In this regard, in this work, the production of electrospun scaffolds was combined with the electrospray of chondrocyte laden hydrogel creating a shield/protection around the cells during and after the electrospray process, preventing its dehydration. For that, a polymeric solution of polycaprolactone (PCL) and gelatin was electrospun alternately with a chondrocyte-laden sodium alginate hydrogel electrospray. Sodium alginate is a natural polymer widely used in biomedical engineering due to its biocompatibility, biodegradability and ability to form hydrogels [5]. The prepared scaffolds were then cultured for 7 days and the respective cell viability assessed. The percentage of viability was calculated as a ratio of the metabolic activity of the electrosprayed chondrocytes and the metabolic activity of chondrocytes that did not underwent any process. The chondrocyte distribution was also evaluated. On the first day of culture, the results showed that the cellular viability was higher than the one previous reported, demonstrating that the alginate hydrogel allowed the cells to survive and helps in its attachment. After 7 days of culture, cells continue alive with considerable viability increasing. It was also shown that it was possible to incorporate cells homogenously distributed by electrospraying process using the chondrocyte laden hydrogel. These results emphasize the potential value that the hydrogels can have on the electrospraying process with the electrospun scaffolds.publishe
Integrated biomimetic carbon nanotube composites for in vivo systems
CESAMAs interest in using carbon nanotubes for developing biologically compatible systems continues to grow, biological inspiration is stimulating new directions for in vivo approaches. The ability to integrate nanotechnology-based systems in the body will provide greater successes if the implanted material is made to mimic elements of the biological milieu especially through tuning physical and chemical characteristics. Here, we demonstrate the highly successful capacity for in vivo implantation of a new carbon nanotube-based composite that is, itself, integrated with a hydroxyapatite-polymethyl methacrylate to create a nanocomposite. The success of this approach is grounded in finely tailoring the physical and chemical properties of this composite for the critical demands of biological integration. This is accomplished through controlling the surface modification scheme, which affects the interactions between carbon nanotubes and the hydroxyapatite-polymethyl methacrylate. Furthermore, we carefully examine cellular response with respect to adhesion and proliferation to examine in vitro compatibility capacity. Our results indicate that this new composite accelerates cell maturation through providing a mechanically competent bone matrix; this likely facilitates osteointegration in vivo. We believe that these results will have applications in a diversity of areas including carbon nanotube, regeneration, chemistry, and engineering research.NANO/NMed-AT/0115/2007SFRH/BPD/14677/2003FC
Effects of the inoculation with soil microbiota onmaize grown in saline soils
food and energetic needs will thus increase dramatically, while conventional agriculture is, even actually, facing drastic reductions in production yields and/or severe increases in cost to compensate losses in productivity due to lower soil fertility
Bio-electrospraying assessment toward in situ chondrocyte-laden electrospun scaffold fabrication
Electrospinning has been widely used to fabricate fibrous scaffolds for cartilage tissue engineering, but their small pores severely restrict cell infiltration, resulting in an uneven distribution of cells across the scaffold, particularly in three-dimensional designs. If bio-electrospraying is applied, direct chondrocyte incorporation into the fibres during electrospinning may be a solution. However, before this approach can be effectively employed, it is critical to identify whether chondrocytes are adversely affected. Several electrospraying operating settings were tested to determine their effect on the survival and function of an immortalized human chondrocyte cell line. These chondrocytes survived through an electric field formed by low needle-to-collector distances and low voltage. No differences in chondrocyte viability, morphology, gene expression, or proliferation were found. Preliminary data of the combination of electrospraying and polymer electrospinning disclosed that chondrocyte integration was feasible using an alternated approach. The overall increase in chondrocyte viability over time indicated that the embedded cells retained their proliferative capacity. Besides the cell line, primary chondrocytes were also electrosprayed under the previously optimized operational conditions, revealing the higher sensitivity degree of these cells. Still, their post-electrosprayed viability remained considerably high. The data reported here further suggest that bio-electrospraying under the optimal operational conditions might be a promising alternative to the existent cell seeding techniques, promoting not only cells safe delivery to the scaffold, but also the development of cellularized cartilage tissue constructs.publishe
- …