Landings and discards on the pulse trawl MFV "Vertrouwen"TX68 in 2009

The catches in terms of landings and discards were monitored onboard MFV “Vertrouwen” TX68, fishing with two pulse trawls using the Verburg-Holland system during four weeks in June-August 2009. The average fishing speed was about 5 knots. The fishing area of the four trips was east of the coast of England and fishing depth was 36m on average with a minimum depth of 20 m and a maximum depth of 46 m. For this study the standard sampling procedure for the yearly monitoring of discards of conventional beam trawl fleet was applied (van Helmond and van Overzee, 2008). Sampled numbers of fish per haul were raised to numbers and weight per hour, for both. When compared with conventional beam trawls in previous years it seems that with the pulse trawl more sole in number and weights per unit of time was discarded and less plaice was discarded. However, the average discard percentages of as well plaice as sole for the pulse trawl of this study were within range with the average discard percentages of conventional beam trawls in 2005, 2006 and 2007 (van Keeken, 2006; van Helmond and van Overzee, 2007; van Helmond and van Overzee, 2008) . This study gives a general impression of the performance in terms of catches of fishing with a pulse trawl using the Verburg-Holland system. However it is recommended to conduct a comparative study on performance of a beam trawl and a pulse trawl, where the two vessels of similar size fish simultaneously, like was done in 2006 by van Marlen et al. This is to exclude the effects of time and area of fishing

AIR KELAPA HIJAU MENURUNKAN DISMENORE PADA REMAJA PUTRI

Menstrual pain or commonly known as dysmenorrhea is muscle cramps felt in the lower abdomen that appear before or during menstruation (menstruation). Menstrual pain is caused by the muscles of the uterine wall contracting, compressing the surrounding blood vessels, as a result, the oxygen supply to the uterus is blocked and triggers pain. The purpose of this study was to determine the effect of giving green coconut water in reducing dysmenorrhoea pain in adolescent girls in the Stikes Dormitory of Pasapua Ambon. This study uses a pre-design experimental design with static group comparison: randomized control group only. The results showed that there was an effect of giving green coconut water to dysmenorrhea in adolescent girls in the Stikes Dormitory of Pasapua Ambon with p = 0.000 (p <0.05). The conclusion is that there is an effect of giving green coconut water on dysmenorrhea in adolescent girls in the STIKES Pasapua Ambon dormitor

Laboratory study of the impact of repetitive electrical and mechanical stimulation on brown shrimp Crangon crangon

Pulse trawling is currently the best available alternative to beam trawling in the brown shrimp Crangon crangon and Sole Solea solea (also known as Solea vulgaris) fisheries. To evaluate the effect of repetitive exposure to electrical fields, brown shrimp were exposed to the commercial electrodes and pulse settings used to catch brown shrimp (shrimp startle pulse) or Sole (Sole cramp pulse) 20 times in 4 d and monitored for up to 14 d after the first exposure. Survival, egg loss, molting, and the degree of intranuclear bacilliform virus (IBV) infection were evaluated and compared with those in stressed but not electrically exposed (procedural control) and nonstressed, nonexposed (control) brown shrimp as well as brown shrimp exposed to mechanical stimuli. The lowest survival at 14 d (57.3%) occurred in the Sole cramp pulse treatment, and this was significantly lower than in the group with the highest survival, the procedural control (70.3%). No effect of electrical stimulation on the severity of IBV infection was found. The lowest percentage of molts occurred in the repetitive mechanical stimulation treatment (14.0%), and this was significantly lower than in the group with the highest percentage of molts, the procedural control (21.7%). Additionally, the mechanically stimulated brown shrimp that died during the experiment had a significantly larger size than the surviving individuals. Finally, no effect of the shrimp startle pulse was found. Therefore, it can be concluded that repetitive exposure to a cramp stimulus and mechanical stimulation may have negative effects on the growth and/or survival of brown shrimp. However, there is no evidence that electrical stimulation during electrotrawls would have a larger negative impact on brown shrimp stocks than mechanical stimulation during conventional beam trawling

Effects of Additives PROBIOLACTlL® and IHPLUS® on Suckling Piglets

Context: Lactation is one on the most critical stages of swine breeding due to the frequent presence of diseases. Aim: To evaluate the probiotic effect of biopreparations PROBIOLACTlL® and IHPLUS® on suckling piglets. Method: The study relied on a completely randomized experimental design, which included three treatments: I. Basal diet (Control); II Basal diet + PROBIOLACTIL®; III. Basal diet + IHPlus®. Productive indicators like live weight, weight gain, mean daily gain, food conversion, and health indicators were evaluated. Results: The additives improved all the indicators evaluated and brought about benefits (P&lt;0.05) in live weight (6.88, 7.63, 7.77 kg); mean daily gain (0.207, 0.281, 0.299 g); weight gain (1.06, 1.38, 1.50 kg); and feed conversion (0.29, 0.25, 0.20). Additionally, there was a drop in the occurrence of diarrhea (33.33, 3.88, 2.77%) observed in the animals treated. Conclusions: The results confirm the probiotic potential of these biopreparations when supplied to the suckling piglets

The basic helix-loop-helix transcription factor TCF4 impacts brain architecture as well as neuronal morphology and differentiation

Germline mutations in the basic helix-loop-helix transcription factor 4 (TCF4) cause the Pitt–Hopkins syndrome (PTHS), a developmental disorder with severe intellectual disability. Here, we report findings from a new mouse model with a central nervous system-specific truncation of Tcf4 leading to severe phenotypic abnormalities. Furthermore, it allows the study of a complete TCF4 knockout in adult mice, circumventing early postnatal lethality of previously published mouse models. Our data suggest that a TCF4 truncation results in an impaired hippocampal architecture affecting both the dentate gyrus as well as the cornu ammonis. In the cerebral cortex, loss of TCF4 generates a severe differentiation delay of neural precursors. Furthermore, neuronal morphology was critically affected with shortened apical dendrites and significantly increased branching of dendrites. Our data provide novel information about the role of Tcf4 in brain development and may help to understand the mechanisms leading to intellectual deficits observed in patients suffering from PTHS

Chemerin-156 is the Active Isoform in Human Hepatic Stellate Cells

The chemokine chemerin exists as C-terminally processed isoforms whose biological functions are mostly unknown. A highly active human chemerin variant (huChem-157) was protective in experimental hepatocellular carcinoma (HCC) models. Hepatic stellate cells (HSCs) are central mediators of hepatic fibrogenesis and carcinogenesis and express the chemerin receptors chemokine-like receptor 1 (CMKLR1) and G protein-coupled receptor 1 (GPR1). Here we aimed to analyse the effect of chemerin isoforms on the viability, proliferation and secretome of the human HSC cell line LX-2. Therefore, huChem-157, 156 and 155 were over-expressed in LX-2 cells, which have low endogenous chemerin levels. HuChem-157 produced in LX-2 cells activated CMKLR1 and GPR1, and huChem-156 modestly induced GPR1 signaling. HuChem-155 is an inactive chemerin variant. Chemerin isoforms had no effect on cell viability and proliferation. Cellular expression of the fibrotic proteins galectin-3 and alpha-smooth muscle actin was not regulated by any chemerin isoform. HuChem-156 increased IL-6, IL-8 and galectin-3 in cell media. HuChem-157 was ineffective, and accordingly, did not enhance levels of these proteins in media of primary human hepatic stellate cells when added exogenously. These analyses provide evidence that huChem-156 is the biologic active chemerin variant in hepatic stellate cells and acts as a pro-inflammatory factor

A comprehensive analysis of prognostic signatures reveals the high predictive capacity of the proliferation, immune response and RNA splicing modules in breast cancer.

INTRODUCTION: Several gene expression signatures have been proposed and demonstrated to be predictive of outcome in breast cancer. In the present article we address the following issues: Do these signatures perform similarly? Are there (common) molecular processes reported by these signatures? Can better prognostic predictors be constructed based on these identified molecular processes? METHODS: We performed a comprehensive analysis of the performance of nine gene expression signatures on seven different breast cancer datasets. To better characterize the functional processes associated with these signatures, we enlarged each signature by including all probes with a significant correlation to at least one of the genes in the original signature. The enrichment of functional groups was assessed using four ontology databases. RESULTS: The classification performance of the nine gene expression signatures is very similar in terms of assigning a sample to either a poor outcome group or a good outcome group. Nevertheless the concordance in classification at the sample level is low, with only 50% of the breast cancer samples classified in the same outcome group by all classifiers. The predictive accuracy decreases with the number of poor outcome assignments given to a sample. The best classification performance was obtained for the group of patients with only good outcome assignments. Enrichment analysis of the enlarged signatures revealed 11 functional modules with prognostic ability. The combination of the RNA-splicing and immune modules resulted in a classifier with high prognostic performance on an independent validation set. CONCLUSIONS: The study revealed that the nine signatures perform similarly but exhibit a large degree of discordance in prognostic group assignment. Functional analyses indicate that proliferation is a common cellular process, but that other functional categories are also enriched and show independent prognostic ability. We provide new evidence of the potentially promising prognostic impact of immunity and RNA-splicing processes in breast cancer.RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are