31 research outputs found
The difference in some aspects of sexsuality between visually impaired and sighted people
Spolnost je dio svih aspekata ljudskoga života i obuhvaÄa velik dio osobnosti Äovjeka. U vezi je s emocionalnim, socijalnim, kulturalnim i tjelesnim rastom pojedinca. Spolnost je prirodan dio ljudskoga razvoja u svakoj životnoj fazi a oÅ”teÄenje vida može na razliÄite naÄine utjecati na spolni život osobe. Ono ne samo da utjeÄe na doživljaj sebe veÄ i na druÅ”tvene i na spolne odnose. Cilj ovoga diplomskog rada jest ispitivanje nekih razlika u obilježjima spolnosti osoba oÅ”teÄena vida i videÄih osoba. Istraživanje je provedeno na ukupno 24 ispitanika/ce od kojih je dvanaest osoba oÅ”teÄena vida i dvanaest videÄih osoba. U skupini osoba oÅ”teÄena vida bilo je Å”est muÅ”kih i Å”est ženskih ispitanika/ica, kao i u skupini videÄih ispitanika u kojoj je takoÄer bilo Å”est muÅ”kih i Å”est ženskih ispitanika/ica. Za potrebe ovog istraživanja izabrani su ispitanici izmeÄu osamnaeste i trideset i pete godine života. Podaci su se prikupljali pomoÄu anketnog upitnika Razlika u nekim obilježjima spolnosti izmeÄu osoba oÅ”teÄena vida i videÄih osoba. Za kvantitativnu obradu podataka koriÅ”ten je SPSS. Rezultati su pokazali da postoje razlike u nekim specifiÄnim obilježjima izmeÄu populacija osoba oÅ”teÄena vida i videÄih osoba u sva tri podruÄja istraživane teme. TakoÄer, uoÄena je razlika u sklapanju novih poznanstava i prijateljstava, partnerskim vezama, stabilnosti veza, seksualnoj orijentaciji, eksperimentiranju s istim spolom i edukacijama o spolnosti.Gender is part of all aspects of human life, and it encompasses a great deal of human personality. It is related to the emotional, social, cultural, and physical growth of an individual. Sexuality is a natural part of human development at every stage of life, and visual impairment can affect the sex life of a person in various ways. It not only has a bearing on oneās personal experiences, but also on oneās social and sexual relationships. The aim of this graduate thesis is to explore some of the characteristics of sexuality in the visually impaired compared to people with normal vision. A total of 24 examinees were surveyed, of whom twelve people had impaired vision, and twelve people had normal vision. In the group of people with impaired vision, there were six male and six female respondents. In the group of people with normal vision, there were also six male, and six female respondents. For the purposes of this study, respondents were selected between the ages of eighteen and thirty-five. The data were collected by means of a questionnaire Differences in some characteristics of sexuality between visually impaired and normal vision persons. SPSS was used for quantitative data processing. The results show that there are some specific differences between the population of visually impaired compared to people with normal vision in all three areas of the research topic. There were also differences in making new acquaintances and friendships, partnerships, relationship stability, sexual orientation, experimentation with the same gender, and sexuality education
Presence of Dichlorodiphenyltrichloroethane (DDT) in Croatia and Evaluation of Its Genotoxicity
The DDT was one of the most commonly used pesticide in mid 20th century and even though its use is banned it is detectible in water, soil, fish, meat and milk products. Using comet assay and micronucleus test we have managed to detect genotoxic properties of this pesticide on human peripheral blood lymphocytes. Results obtained in this research indicate the need for further environmental and food monitoring, and cytogenetic research using sensitive methods in detection of primary genome damage after exposure to DDT to establish the impact of such chemicals on human genome and health
Natural products counteracting cardiotoxicity during cancer chemotherapy: The special case of doxorubicin, a comprehensive review
Cardiotoxicity is a frequent undesirable phenomenon observed during oncological treatment that limits the therapeutic dose of antitumor drugs and thus may decrease the effectiveness of cancer eradication. Almost all antitumor drugs exhibit toxic properties towards cardiac muscle. One of the underlying causes of cardiotoxicity is the stimulation of oxidative stress by chemotherapy. This suggests that an appropriately designed diet or dietary supplements based on edible plants rich in antioxidants could decrease the toxicity of antitumor drugs and diminish the risk of cardiac failure. This comprehensive review compares the cardioprotective efficacy of edible plant extracts and foodborne phytochemicals whose beneficial activity was demonstrated in various models in vivo and in vitro. The studies selected for this review concentrated on a therapy frequently applied in cancer, anthracycline antibioticādoxorubicināas the oxidative stress- and cardiotoxicity-inducing agent
In vitro genotoxicity assessment of a monolacunary Wells-Dawson nanocluster as a promising contrast agent candidate
Tungsten-based nanoparticles possess high density and capability to attenuate X-rays and thus have been studied as interesting candidates for the development of new-generation contrast-enhancing staining agents (CESAs) for computed tomography (CT). Polyoxotungstates, as polyoxoanions containing tungsten in its high oxidation state (W6+) were reported as promising CESA candidates to visualize long bones and kidney tissues. However, some polyoxotungstates induced side effects in toxicity studies in vivo, which could limit their clinical application. Thus, the aim of this study was to evaluate genotoxic effects in vitro of monolacunary Wells-Dawson polyoxotungstate, Ī±2-K10P2W17O61.20H2O (lacunary WD) that was found as a potential contrast agent for CT in our previous studies in situ and in vitro. Lacunary WD was synthesized by following the reported procedure. The genotoxicity evaluation was performed by using the standard procedure for the alkaline comet assay. Briefly, human whole blood samples were taken from healthy male and female donors and exposed to different lacunary WD concentrations within the range of 10-6-10-4 mol/L, for 4 and 24 h at 37 Ā°C. Then, 5 Ī¼L of whole blood was embedded into an agarose matrix and subsequently lysed (2.5 M NaCl, 100 mM EDTANa2, 10 mM Tris, 1% sodium sarcosinate, 1% Triton X-100, 10% DMSO, pH 10) overnight at 4 Ā°C. After the lysis, the slides were placed into an alkaline solution (300 mM NaOH, 1 mM EDTANa2, pH 13) for 20 min at 4 Ā°C to allow DNA unwinding and subsequently electrophoresed for 20 min at 1 V/cm. Finally, the slides were neutralized in 0.4 M Tris buffer (pH 7.5) for 5 min 3 times, stained with ethidium bromide (10 Ī¼g/mL), and analyzed at 250Ć magnification using an epifluorescence microscope (Zeiss, Gƶttingen, Germany) connected through a camera to an image analysis system (Comet Assay II; Perceptive Instruments Ltd., Haverhill, Suffolk, UK). One hundred randomly captured comets from each slide were examined. Multiple comparisons between groups were done by means of ANOVA on log-transformed data. Post hoc analyses of the differences were done by the ScheffĆ© test. The percentage of tail DNA was determined to evaluate the level of DNA damage and genotoxicity potential. The obtained results showed that lacunary WD did not induce a statistically significant relative increase of tail DNA compared to the corresponding control at all investigated concentrations, after both 4 and 24 h exposure. Accordingly, the investigated promising contrast agent candidate could be regarded in further studies as toxicologically safe for healthy human blood cells from a genotoxicity point of view.RAD 2023 : 11th International Conference on Radiation Natural Sciences, Medicine, Engineering, Technology and Ecology (RAD 2023) 19ā23.06.2023; Herceg Novi, Montenegr
The difference in some aspects of sexsuality between visually impaired and sighted people
Spolnost je dio svih aspekata ljudskoga života i obuhvaÄa velik dio osobnosti Äovjeka. U vezi je s emocionalnim, socijalnim, kulturalnim i tjelesnim rastom pojedinca. Spolnost je prirodan dio ljudskoga razvoja u svakoj životnoj fazi a oÅ”teÄenje vida može na razliÄite naÄine utjecati na spolni život osobe. Ono ne samo da utjeÄe na doživljaj sebe veÄ i na druÅ”tvene i na spolne odnose. Cilj ovoga diplomskog rada jest ispitivanje nekih razlika u obilježjima spolnosti osoba oÅ”teÄena vida i videÄih osoba. Istraživanje je provedeno na ukupno 24 ispitanika/ce od kojih je dvanaest osoba oÅ”teÄena vida i dvanaest videÄih osoba. U skupini osoba oÅ”teÄena vida bilo je Å”est muÅ”kih i Å”est ženskih ispitanika/ica, kao i u skupini videÄih ispitanika u kojoj je takoÄer bilo Å”est muÅ”kih i Å”est ženskih ispitanika/ica. Za potrebe ovog istraživanja izabrani su ispitanici izmeÄu osamnaeste i trideset i pete godine života. Podaci su se prikupljali pomoÄu anketnog upitnika Razlika u nekim obilježjima spolnosti izmeÄu osoba oÅ”teÄena vida i videÄih osoba. Za kvantitativnu obradu podataka koriÅ”ten je SPSS. Rezultati su pokazali da postoje razlike u nekim specifiÄnim obilježjima izmeÄu populacija osoba oÅ”teÄena vida i videÄih osoba u sva tri podruÄja istraživane teme. TakoÄer, uoÄena je razlika u sklapanju novih poznanstava i prijateljstava, partnerskim vezama, stabilnosti veza, seksualnoj orijentaciji, eksperimentiranju s istim spolom i edukacijama o spolnosti.Gender is part of all aspects of human life, and it encompasses a great deal of human personality. It is related to the emotional, social, cultural, and physical growth of an individual. Sexuality is a natural part of human development at every stage of life, and visual impairment can affect the sex life of a person in various ways. It not only has a bearing on oneās personal experiences, but also on oneās social and sexual relationships. The aim of this graduate thesis is to explore some of the characteristics of sexuality in the visually impaired compared to people with normal vision. A total of 24 examinees were surveyed, of whom twelve people had impaired vision, and twelve people had normal vision. In the group of people with impaired vision, there were six male and six female respondents. In the group of people with normal vision, there were also six male, and six female respondents. For the purposes of this study, respondents were selected between the ages of eighteen and thirty-five. The data were collected by means of a questionnaire Differences in some characteristics of sexuality between visually impaired and normal vision persons. SPSS was used for quantitative data processing. The results show that there are some specific differences between the population of visually impaired compared to people with normal vision in all three areas of the research topic. There were also differences in making new acquaintances and friendships, partnerships, relationship stability, sexual orientation, experimentation with the same gender, and sexuality education
Cytogenetic and oxidative status of individuals with thyroid gland illnesses
Biomonitoringom genomske cjelovitosti moguÄe je poboljÅ”ati prevenciju raka. Rak Å”titne žlijezde postao je jedan od najbrže rastuÄih tipova raka u svijetu. Usporedbom 100 ispitanika kontrolne skupine s ispitanicima s oboljenjima Å”titne žlijezde uoÄene su viÅ”e razine oÅ”teÄenja molekule DNA u limfocitima periferne krvi (1,24 puta veÄi broj izmjena sestrinskih kromatida, 1,47 puta veÄi ukupni broj kromosomskih aberacija, 1,84 veÄi intenzitet repa, i 2,32 puta veÄi ukupni broj mikronukleusa). Oksidacijski status ispitanika s oboljenjima Å”titne žlijezde takoÄer je naruÅ”en gdje je uoÄena 1,60 puta veÄa koncentracija malondialdehida te 32 % niža koncentracija glutationa. Nadalje, B-Raf i Ret proteini izraženi su u 97,89 % citoplazmi tkiva oboljelih ispitanika Å”to sugerira kako bi se citogenetiÄki biomarkeri mogli koristiti u prevenciji raka Å”titne žlijezde. Pritom bi se oksidacijski stres mogao smatrati jednim od mehanizama nastanka bolesti.Biomonitoring of genome integrity could be used for better cancer prevention. Thyroid cancer has become one of the fastest growing cancer types in the world. When compared to 100 matched controls, patients with thyroid illnesses showed higher basal DNA damage in peripheral blood lymphocytes (1.24-fold increase in sister chromatid exchanges, 1.47-fold increase in total number of chromosomal aberrations, 1.84-fold increase in tail intensity, and 2.32-fold increase in total number of micronuclei). The oxidative status of thyroid patients was also altered showing 1.60-fold increase in concentration of malondialdehyde and 32 % decrease in concentration of glutathione. Finally, the observed B-Raf and Ret in 97.89 % of patientās tissue cytoplasms suggest that cytogenetic biomarkers could be used in thyroid cancer prevention. Additionally, oxidative stress might be one of the mechanisms of thyroid disease initiation