The effect of optical substrates on micro-FTIR analysis of single mammalian cells

The study of individual cells with infrared (IR) microspectroscopy often requires living cells to be cultured directly onto a suitable substrate. The surface effect of the specific substrates on the cell growth—viability and associated biochemistry—as well as on the IR analysis—spectral interference and optical artifacts—is all too often ignored. Using the IR beamline, MIRIAM (Diamond Light Source, UK), we show the importance of the substrate used for IR absorption spectroscopy by analyzing two different cell lines cultured on a range of seven optical substrates in both transmission and reflection modes. First, cell viability measurements are made to determine the preferable substrates for normal cell growth. Successively, synchrotron radiation IR microspectroscopy is performed on the two cell lines to determine any genuine biochemically induced changes or optical effect in the spectra due to the different substrates. Multivariate analysis of spectral data is applied on each cell line to visualize the spectral changes. The results confirm the advantage of transmission measurements over reflection due to the absence of a strong optical standing wave artifact which amplifies the absorbance spectrum in the high wavenumber regions with respect to low wavenumbers in the mid-IR range. The transmission spectra reveal interference from a more subtle but significant optical artifact related to the reflection losses of the different substrate materials. This means that, for comparative studies of cell biochemistry by IR microspectroscopy, it is crucial that all samples are measured on the same substrate type. [Figure: see text

Saturation of intersubband transitions in p-doped GaAs/AlGaAs quantum wells

Optical saturation experiments have been performed on hh1-hh2 intersubband transitions in two samples of p-doped GaAs/AlGaAs quantum wells. The transitions had energies of 183 and 160 meV and the measured population relaxation times were 2±1.5 and 0.3±0.1 ps, respectively. Modeling of the quantum wells with a 6×6 k·p method shows that intersubband scattering by LO phonons can account for these relaxation times. The valence bandstructure is typically more complicated than the conduction bandstructure in a quantum well but these measurements show that LO phonons are the dominant intersubband scattering mechanism in both cases

Tracking Thermal-Induced Amorphization of a Zeolitic Imidazolate Framework via Synchrotron In Situ Far-Infrared Spectroscopy

We present the first use of in situ far-infrared spectroscopy to analyze the thermal amorphization of a zeolitic imidazolate framework material. We explain the nature of vibrational motion changes during the amorphization process and reveal new insights into the effect that temperature has on the Zn-N tetrahedra.Comment: 5 pages, 3 figures, 2 table

Vegetation and climate change on the Bolivian Altiplano between 108,000 and 18,000 years ago

A 90,000-yr record of environmental change before 18,000 cal yr B.P. has been constructed using pollen analyses from a sediment core obtained from Salar de Uyuni (3653 m above sea level) on the Bolivian Altiplano. The sequence consists of alternating mud and salt, which reflect shifts between wet and dry periods. Low abundances of aquatic species between 108,000 and 50,000 yr ago (such as Myriophyllum and Isoëtes) and marked fluctuations in Pediastrum suggest generally dry conditions dominated by saltpans. Between 50,000 yr ago and 36,000 cal yr B.P., lacustrine sediments become increasingly dominant. The transition to the formation of paleolake “Minchin” begins with marked rises in Isoëtes and Myriophyllum, suggesting a lake of moderate depth. Similarly, between 36,000 and 26,000 cal yr B.P., the transition to paleolake Tauca is also initiated by rises in Isoëtes and Myriophyllum; the sustained presence of Isoëtes indicates the development of flooded littoral communities associated with a lake maintained at a higher water level. Polylepis tarapacana-dominated communities were probably an important component of the Altiplano terrestrial vegetation during much of the Last Glacial Maximum (LGM) and previous wet phases

Reactions of Dimethylether in Single Crystals of the Silicoaluminophosphate STA-7 Studied via Operando Synchrotron Infrared Microspectroscopy

Open access via the Springer Compact Agreement. We thank the Diamond Light Source for provision of beam time and support facilities at the MIRIAM beamline B22 (Experiments SM11766-1 and SM13725-1). Financial support from the EPSRC Catalysis Hub (Suwardiyanto) and an Industrial CASE Award (EPSRC/BP Chemicals) (Price) are also acknowledged.Peer reviewedPublisher PD