2 research outputs found
Contribution of high energy physics techniques to the medical imaging field
The purpose of this study was to show how advanced concepts of compact,
lossless and "Time Of Flight" (TOF) capable electronics similar to those
foreseen for the LHC and ILC experiments could be fairly and easily transferred
to the medical imaging field through Positron Emission Tomography (PET). As a
wish of explanation, the two overriding weaknesses of PET camera readout
electronics, namely dead-time and timing resolution, were investigated
analytically and with a Monte-Carlo simulator presently dedicated to this task.
Results have shown there was room left for count rate enhancement through a
huge decrease of the timing resolution well below the nanosecond. The novel
electronics scheme suggested for PET in this paper has been partly inspired by
the long experience led in High Energy Physics where the latter requirement is
compulsory. Its structure entirely pipelined combined to a pixelation of the
whole detector should allow dead-times to be suppressed, while the absence of
devoted timing channel would remove the preponderant contributions to the
timing resolution. To the common solution for timing would substitute an
optimal filtering method witch clearly appears as a good candidate as timing
resolution of a few tens of picoseconds may be achieved provided the shape of
the signal is known and sufficient samples are available with enough accuracy.
First investigations have yield encouraging results as a sampling frequency of
50 MHz with a 7 bits precision appears sufficient to ensure the 500ps
coincidence timing resolution planed. At this point, there will be a baby step
ahead to draw benefice from a TOF implementation to the design and the enormous
noise variance enhancement that would come with.Comment: presented at EuroMedIm 2006 : 1st European Conference on Molecular
Imaging Technology, Marseille 9-12 May 2006, 6 pp, 4 figures, submitted to
NI
Broadening the antibacterial spectrum of histidine kinase autophosphorylation inhibitors via the use of epsilon-poly-L-lysine capped mesoporous silica-based nanoparticles
[EN] Two-component systems (TCS) regulate diverse processes such as virulence, stress responses, metabolism and antibiotic resistance in bacteria but are absent in humans, making them promising targets for novel antibacterials. By incorporating recently described TCS histidine kinase autophosphorylation inhibitors (HKAIs) into epsilon-poly-L-lysine capped nanoparticles (NPs) we could overcome the Gram negative (Gr(-)) permeability barrier for the HKAIs. The observed bactericidal activity against Gr(-) bacteria was shown to be due to the enhanced delivery and internalization of the HKAIs and not an inhibitory or synergistic effect of the NPs. The NPs had no adverse effects on mammalian cell viability or the immune function of macrophages in vitro and showed no signs of toxicity to zebrafish larvae in vivo. These results show that HKAIs are promising antibacterials for both Gr(-) and Gr + pathogens and that NPs are a safe drug delivery technology that can enhance the selectivity and efficacy of HKAIs against bacteria. (C) 2016 Elsevier Inc. All rights reserved.This work was funded by FP7 ITN STARS-Scientific Training in Antimicrobial Research Strategies (Contract No. PITN-GA-2009-238490, J.M.W., A.M.), H2020 MSCA IF (AND-659121, N.V.), grant BIO2013-42619-P from the Ministerio de Economia y Competitividad (A.M.), grant from the Spanish Government (Project MAT2015-64139-C4-1-R,N. M., J.R.M, R.M.M.), and a grant from Generalitat Valenciana (Project PROMETEOII/2014/047, N.M.). and Prometeo II/2014/029, A.M.).Velikova, N.; Mas Font, N.; Miguel-Romero, L.; Polo, L.; Stolte, E.; Zaccaria, E.; Cao, R.... (2017). Broadening the antibacterial spectrum of histidine kinase autophosphorylation inhibitors via the use of epsilon-poly-L-lysine capped mesoporous silica-based nanoparticles. Nanomedicine Nanotechnology Biology and Medicine. 13(2):569-581. https://doi.org/10.1016/j.nano.2016.09.011S56958113