Minimal Information About Sample Preparation for Phosphoproteomics

This guideline describes parameters and conditions involved in phosphopeptide sample preparation. It covers from the description and preparation of the cells and tissues to the fractionation and specific enrichment of phosphopeptides for MS analysis. The guideline is prepared in order to easily cope with many of the experimental designs used in phosphoproteomic studies. 
 
The document is subdivided as follows:
1. General features
2. Sample processing
3. Protein Purification/Fractionation
4. Peptide Purification/Fractionation
5. Phosphopeptide enrichment
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Characterization of dializated proteins in a mars system

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Characterization of peptides and proteins in therapeutic human serum albumin

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Quantitative characterization of p-sites from the human t-lymphocyte phosphoproteome using isotopic labeling and mass spectrometry

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Herramientas Bioinformáticas en el Laboratorio de Proteómica CSIC/UAB

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La acción individual promovida por acreedores en supuestos de cierre de hecho

El presente trabajo aborda el ejercicio de la acción individual de responsabilidad contra los administradores societarios regulada en el art. 241 de la Ley de Sociedades de Capital con el objeto de reclamar deudas dinerarias tras el cierre de hecho de la sociedad deudora.Departamento de Derecho Mercantil, Derecho del Trabajo e Internacional PrivadoMáster en Abogací

Requisitos de protección del diseño industrial. En particular, el conflicto con las marcas.

El presente trabajo aborda los requisitos de novedad y carácter singular exigidos para obtener la protección legal del diseño industrial desde la perspectiva nacional y europea. Además, analiza los límites de la protección del diseño industrial frente al Derecho de marcas y los posibles conflictos entre ambas modalidades de protección.Departamento de Derecho Mercantil, Derecho del Trabajo e Internacional PrivadoGrado en Derech

Alcohol-based mouthwash as a risk factor of oral cancer: A systematic review

Background: Oral and pharynx cancer represent a serious global problem, reaching an incidence of half a million cases annually. The role of tobacco and alcohol have been studied and proven to be one of its risk factors. We also know that mouthwashes contain a variable percentage of alcohol, so there is a reasonable concern about their role in carcinogenesis. Materials and Methods: To answer the PICOS (Population; Intervention; Comparison; Outcomes; Study) question: "Do patients (Population) who use alcohol-based mouthwashes (Intervention) compared to those who do not use them (Comparison) have higher acetaldehyde levels in saliva or higher risk of oral cancer development? (Out- comes)" Meta-analyses, systematic reviews, randomized and non-randomized clinical trials, case-control stud- ies, and prospective and retrospective cohort studies were included (Study). Two independent authors conducted literature screening through MEDLINE, Scopus and the Cochrane Library, and they also conducted article and data extraction to undertake quality analyses. The main outcome measures were salivary acetaldehyde levels or the risk of oral cancer development. The most relevant data was extracted and the risk of bias from the studies included was also evaluated. Results: Out of 497 potentially eligible papers, 8 studies were included in the qualitative analysis which include a total of 43,499 subjects: two meta-analyses, a clinical trial, three case-control studies and two cohort studies. One study (n = 3,926) found a relationship between alcohol mouthwash and oral cancer, two studies (n = 25,033) found this relationship when a high frequency of mouthwash was present, three studies (n = 14,482) failed to find this rela- tionship and 2 studies (n = 58) found a temporary increase of acetaldehyde levels in saliva after alcohol mouthwash. Conclusions: It cannot be guaranteed that the use of mouthwash represents an independent risk factor for the develop- ment of head and neck cancer. However, the risk does increase when it occurs in association with other carcinogenic risk factors

Sesión de modificaciones postraduccionales

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