D5.9 - FAIR-oriented research data management: Support, Training and Assessment Activity Report update}

This deliverable reports on the activities and results of Tasks 5.3, Helpdesk, and documentation for promoting FAIR practices and support to FAIR oriented data stewardship, and Task 5.4, Training modules on FAIR oriented research data management tools and solutions, during the second reporting period (January 1st, 2021, to December 31st, 2022). The document provides an update and builds upon the results reported in D5.

Developing the EOSC-pillar RDM training and support catalogue

Today's many research infrastructures and European projects offer training catalogues to store and list multiple forms of learning materials. In EOSC Pillar project we propose a web application catalogue, which consists of training materials as well as day-to-day operational resources with the aim to support data stewards and other RDM(research data management), FAIR data (findable, accessible, interoperable, reusable) and open science actors. In this paper we briefly describe the scope and technical implementation of the EOSC-Pillar RDM Training and Support Catalogue and how we are addressing current challenges such as metadata standards, controlled vocabularies, curation, quality checking and sustainability

Les syndicats britanniques : déclin ou renouveau?

Раздзел VI. Медыялінгвістыка: сучасная парадыгма навуковых ведаў і рэдактарскі вопытАб’ектам даследавання выступае канцэпт «Беларусь», прадметам – медыявербалізацыя яго ў форме персаніфікацыі. Асноўным метадам даследавання з’яўляецца семантыка­кагнітыўны аналіз. Уклад аўтара ў вывучэнне тэмы заключаецца ў тым, што падчас разгляду вербалізацыі прэдыкатыўнай спалучальнасці канцэпту «Беларусь» выяўляюцца асаблівасці яго функцыянавання ў медыя.Даследаванне выканана пры фінансавай падтрымцы БРФФД у рамках навуковага праекта «Медыялінгвістыка ў Беларусі: умовы фарміравання, асаблівасці развіцця і сучасны стан» (дамова з БРФФД № Г18­062 ад 30.05.2018 г.).The object of the study is the concept «Belarus», the subject is its verbalization in the media in the form of personification. The main method of research of the concept «Belarus» is semantic­cognitive analysis. The author’s contribution to the study of the topic is that during the analysis of the verbalization of the predictive compatibility of the concept «Belarus» the peculiarities of its functioning in the media are established

Increased Prevalence of <i>Methanosphaera stadtmanae</i> in Inflammatory Bowel Diseases

<div><p>Background</p><p>The gut microbiota is associated with the modulation of mucosal immunity and the etiology of inflammatory bowel diseases (IBD). Previous studies focused on the impact of bacterial species on IBD but seldom suspected archaea, which can be a major constituent of intestinal microbiota, to be implicated in the diseases. Recent evidence supports that two main archaeal species found in the digestive system of humans, <i>Methanobrevibacter smithii</i> (MBS) and <i>Methanosphaera stadtmanae</i> (MSS) can have differential immunogenic properties in lungs of mice; with MSS but not MBS being a strong inducer of the inflammatory response. We thus aimed at documenting the immunogenic potential of MBS and MSS in humans and to explore their association with IBD.</p><p>Methods</p><p>To validate the immunogenicity of MBS and MSS in humans, peripheral blood mononuclear cells from healthy subjects were stimulated with these two microorganisms and the production of inflammatory cytokine TNF was measured by ELISA. To verify MBS and MSS prevalence in IBD, stool samples from 29 healthy control subjects and 29 patients suffering from IBD were collected for DNA extraction. Plasma was also collected from these subjects to measure antigen-specific IgGs by ELISA. Quantitative PCR was used for bacteria, methanogens, MBS and MSS quantification.</p><p>Results</p><p>Mononuclear cells stimulated with MSS produced higher concentrations of TNF (39.5 ng/ml) compared to MBS stimulation (9.1 ng/ml). Bacterial concentrations and frequency of MBS-containing stools were similar in both groups. However, the number of stool samples positive for the inflammatory archaea MSS was higher in patients than in controls (47% vs 20%). Importantly, only IBD patients developed a significant anti-MSS IgG response.</p><p>Conclusion</p><p>The prevalence of MSS is increased in IBD patients and is associated with an antigen-specific IgG response.</p></div

IgG response in plasma of controls subjects and IBD patients.

<p>Titers of A) MBS- and B) MSS-specific IgGs. C) Quantification of total IgGs. Results are expressed as average ± SEM. Eighteen controls (9 positive for MBS and 4 positive for MSS in stool samples) and seventeen patients (6 positive for MBS and 9 positive for MSS in stool samples) were analyzed. p<0.05.</p

Comparison of the presence of MSS and/or MBS in stool samples from control subjects and patients.

<p>Results are expressed as numbers of positive samples for MSS and/or MBS for each group. Twenty-nine subjects per group were analyzed. P<.05 when patients were compared to controls for prevalence distribution of MBS and MSS.</p

Clinical status of recruited patients.

<p>Clinical status of recruited patients.</p

Primers and probes used in the study.

<p>Primers and probes used in the study.</p