DEVELOPMENTAL PSYCHOPATHOLOGY IN THE COVID-19 PERIOD. COVID-19 PANDEMIC IMPACT ON CHILDREN AND ADOLESCENTS\u27 MENTAL HEALTH

Over the past year, the novel coronavirus (COVID-19) pandemic has forced many world countries, including Italy, to take strict restrictive measures as lockdown and social distancing. Children and adolescents exposed to the COVID-19 pandemic and social distancing would appear to be at greater risk of developing psychiatric disorders. In the last year, the Child and Adolescence Neuropsychiatry service at the Children\u27s Hospital Bambino Gesù in Rome has recorded a significant increase in cases of mood disorders, self-injurious behaviors and suicidal ideation. These data underlined the need to define tailor-made intervention strategies for children and adolescents during this time of social and health emergency

Spatial ability and handedness as potential predictors of space teleoperation performance

Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Aeronautics and Astronautics, 2010.This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.Cataloged from student submitted PDF version of thesis.Includes bibliographical references (p. 60-61).NASA is concerned with finding performance predictors for space teleoperation tasks in order to improve training efficiency. Experiment 1 determined whether scores on tests of spatial skills could predict performance when selecting camera views for a simulated teleoperation task. The hypothesis was that subjects with high spatial ability would perform camera selection tasks more quickly and accurately than those with lower spatial ability, as measured by the Mental Rotation Test (MRT), Purdue Spatial Visualization Test (PSVT), and the Perspective Taking Ability (PTA) test. Performance was defined by task time, number of correct camera selections, preparation time, number of camera changes, and correct identification of clearance issues. Mixed regression and nonparametric tests showed that high-scoring subjects on the MRT and PTA spatial ability tests had significantly lower task times, higher camera selection scores, and fewer camera changes than subjects with lower scores, while High PSVT scorers had significantly lower preparation times. Experiment 2 determined whether spatial ability, joystick configuration, and handedness influenced performance of telerobotic fly-to tasks in a virtual ISS environment. 11 righthanded and 9 left-handed subjects completed 48 total trials, split between two hand controller configurations. Performance was defined by task time, percentage of translational and rotational multi-axis movement, percentage of bimanual movement, and number of discrete movements. High scorers for the MRT, PSVT, and PTA tests had lower Task Times, and High PSVT and PTA scorers made fewer Discrete Movements than Low scorers. High MRT and PTA scorers had a higher percentage of translational and rotational multi-axis movement, and High MRT scorers had a higher percentage of bimanual movement. The overall learning effect appears to be greater than the effect of switching between hand controller configurations. No significant effect of handedness was found. These results indicate that these spatial ability tests could predict performance on space teleoperation tasks, at least in the early phases of training. This research was supported by the National Space Biomedical Research Institute through NASA NCC 9- 58.by Teresa Maria Pontillo.S.M

Failure to learn a new spatial format in children with developmental dyslexia

A general problem in studying children with developmental dyslexia is how to separate inefficiency in learning on the one hand from exposure to written texts on the other. To evaluate dyslexic children's learning abilities with graphemic materials, we tested their improvement in a condition that minimized previous experience with words (i.e., "novel words") and with the standard, horizontal spatial letter array (i.e., a non-canonical "zigzag" format). We selected five pairs of children with dyslexia and (younger) typically developing readers matched for reading speed and accuracy in these specific conditions. Reading performance on novel words in the zigzag format was measured in 23 sessions; learning curves were fitted by power functions. Similar to typically developing readers, children with dyslexia improved their reading of novel words presented in the new format; however, their rate of learning was slower than that of typically developing readers. Furthermore, their learning to read in the new format did not generalize to novel untrained items, whereas significant generalization was present in typically developing readers. As the failure to generalize learning of the spatial format could not be attributed to reduced experience, it indicates a genuine disability and points to impaired perceptual learning as a factor in developmental dyslexia

Gold-based drug encapsulation within a ferritin nanocage: X-ray structure and biological evaluation as a potential anticancer agent of the Auoxo3-loaded protein

Auoxo3, a cytotoxic gold(iii) compound, was encapsulated within a ferritin nanocage. Inductively coupled plasma mass spectrometry, circular dichroism, UV-Vis absorption spectroscopy and X-ray crystallography confirm the potential-drug encapsulation. The structure shows that naked Au(i) ions bind to the side chains of Cys48, His49, His114, His114 and Cys126, Cys126, His132, His147. The gold-encapsulated nanocarrier has a cytotoxic effect on different aggressive human cancer cells, whereas it is significantly less cytotoxic for non-tumorigenic cells

Hallucinations in the Child and Adolescent "Ultra-High Risk" population: A Systematic Review

Abstract Background and Hypothesis "Ultra-high risk" for psychosis young adults are assumed to be at higher risk of developing a psychotic spectrum disorder. Predominantly, the ultrahigh-risk population is aged 18–35 years, but it may also include younger children and adolescents. Individuals in this population experience psychosis prodromes in the form of attenuated or brief psychotic symptoms (particularly perceptual abnormalities). Albeit diagnosis is made via structured interviews, such measures fail to sufficiently assess the precise form and content of perceptual abnormalities, especially as they manifest in children and adolescents. Study Design The present study involved a systematic review of the literature on perceptual abnormalities (particularly hallucinations) in ultrahigh-risk children and adolescents. Results The analysis reviewed five studies and drew conclusions about the perceptual abnormalities (ie, hallucinations) experienced by the study samples, focusing on form, content, and associations with other symptoms. Of note, 2 of the investigated studies suggested a relationship between hallucinations and experiences of childhood trauma. Conclusions The transition to psychosis and experiences of childhood trauma could correspond to different types of hallucinations in ultrahigh-risk children and adolescents. This knowledge could improve the identification of prodromal states in the young, ultrahigh-risk population

Progressive multifocal leukoencephalopathy presenting with bilateral myoclonus: a case report

Progressive multifocal leukoencephalopathy (PML) is a demyelinating disease of the central nervous system (CNS) caused by John Cunningham virus lytic infection of the oligodendrocytes, the myelin-producing cells in the CNS. Symptoms largely vary depending on location and size of the lesions, and the most frequent clinical presentation is characterized by motor deficits, altered consciousness, gait ataxia, and visual symptoms. Despite limb weakness or hemiparesis as the most frequent presenting symptom, involuntary movement is far less common, and very few cases are described in the literature with focal movement disorders without additional neurologic abnormalities. Here we described a case of PML in a patient treated for non-Hodgkin lymphoma with immunomodulatory chemotherapies who presented with bilateral myoclonus of the upper limbs. This report highlights the importance of considering PML in the differential diagnosis of focal movement disorders and discusses the potential causative mechanism of this atypical presentation

Evaluation of Disability Progression in Multiple Sclerosis via Magnetic-Resonance-Based Deep Learning Techniques

Short-term disability progression was predicted from a baseline evaluation in patients with multiple sclerosis (MS) using their three-dimensional T1-weighted (3DT1) magnetic resonance images (MRI). One-hundred-and-eighty-one subjects diagnosed with MS underwent 3T-MRI and were followed up for two to six years at two sites, with disability progression defined according to the expanded-disability-status-scale (EDSS) increment at the follow-up. The patients' 3DT1 images were bias-corrected, brain-extracted, registered onto MNI space, and divided into slices along coronal, sagittal, and axial projections. Deep learning image classification models were applied on slices and devised as ResNet50 fine-tuned adaptations at first on a large independent dataset and secondly on the study sample. The final classifiers' performance was evaluated via the area under the curve (AUC) of the false versus true positive diagram. Each model was also tested against its null model, obtained by reshuffling patients' labels in the training set. Informative areas were found by intersecting slices corresponding to models fulfilling the disability progression prediction criteria. At follow-up, 34% of patients had disability progression. Five coronal and five sagittal slices had one classifier surviving the AUC evaluation and null test and predicted disability progression (AUC > 0.72 and AUC > 0.81, respectively). Likewise, fifteen combinations of classifiers and axial slices predicted disability progression in patients (AUC > 0.69). Informative areas were the frontal areas, mainly within the grey matter. Briefly, 3DT1 images may give hints on disability progression in MS patients, exploiting the information hidden in the MRI of specific areas of the brain

Hexachlorobenzene promotes angiogenesis in vivo, in a breast cancer model and neovasculogenesis in vitro, in the human microvascular endothelial cell line HMEC-1

Exposure to environmental pollutants may alter proangiogenic ability and promotes tumor growth. Hexachlorobenzene (HCB) is an organochlorine pesticide found in maternal milk and in lipid foods, and a weak ligand of the aryl hydrocarbon receptor (AhR). HCB induces migration and invasion in human breast cancer cells, as well as tumor growth and metastasis in vivo. In this study, we examined HCB action on angiogenesis in mammary carcinogenesis. HCB stimulates angiogenesis and increases vascular endothelial growth factor (VEGF) expression in a xenograft model with the human breast cancer cell line MDA-MB-231. Human microvascular endothelial cells HMEC-1 exposed to HCB (0.005, 0.05, 0.5 and 5 μM) showed an increase in cyclooxygenase-2 (COX-2) and VEGF protein expression involving AhR. In addition, we found that HCB enhances VEGF-Receptor 2 (VEGFR2) expression, and activates its downstream pathways p38 and ERK1/2. HCB induces cell migration and neovasculogenesis in a dose-dependent manner. Cells pretreatment with AhR, COX-2 and VEGFR2 selective inhibitors, suppressed these effects. In conclusion, our results show that HCB promotes angiogenesis in vivo and in vitro. HCB-induced cell migration and tubulogenesis are mediated by AhR, COX-2 and VEGFR2 in HMEC-1. These findings may help to understand the association among HCB exposure, angiogenesis and mammary carcinogenesis.Fil: Pontillo, Carolina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; ArgentinaFil: Español, Alejandro Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Chiappini, Florencia Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Miret, Noelia Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; ArgentinaFil: Cocca, Claudia Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Físico Matemática; ArgentinaFil: Alvarez, Laura. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; ArgentinaFil: Kleiman, Diana Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; ArgentinaFil: Sales, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Randi, Andrea Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentin

Mechanical design of the echinoid test and its biomimetic potentialities

Echinoids, known as sea urchins, are invertebrates populating the seas since the late Ordovician. During their evolution, they underwent a relevant adaptive radiation leading to a variety of specialized forms and lifestyles. Most of the evolutionary success of echinoids is certainly due to the strategic employment of their endoskeleton, with particular reference to the coherent shell-structure of the test adapted to resist both biotic and abiotic stresses related to marine environments. This optimized structure, which minimizes both energy and materials employed, could also represent an ideal model for transferring functional bio-inspired solutions to building constructions and different industrial sectors. The present contribution intends to provide a short overview on the echinoid strategic morpho-functional adaptation of the test and its potential in the biomimetic field.Los equinoides, conocidos como erizos de mar, son invertebrados que pueblan los mares desde finales del Ordovícico. Durante su evolución, se sometieron a una radiación adaptativa relevante que condujo a una variedad de formas y estilos de vida especializados. La mayor parte del éxito evolutivo de los equinoides se debe sin duda al empleo estratégico de su endoesqueleto, con especial referencia a la estructura coherente de la capa de la prueba adaptada para resistir las tensiones bióticas y abióticas relacionadas con los entornos marinos. Esta estructura optimizada, que minimiza tanto la energía como los materiales empleados, también podría representar un modelo ideal para transferir soluciones funcionales bioinspiradas a construcciones de edificios y diferentes sectores industriales. La presente contribución pretende proporcionar una breve descripción de la adaptación morfofuncional estratégica equinoide de la prueba y su potencial en el campo biomimético.Equinóides, conhecidos como ouriços-do-mar, são invertebrados que povoam os mares desde o final do Ordoviciano. Durante sua evolução, eles sofreram uma radiação adaptativa relevante levando a uma variedade de formas e estilos de vida especializados. A maior parte do sucesso evolutivo dos equinoides é certamente devido ao emprego estratégico de seu endoesqueleto, com particular referência à estrutura de concha coerente do teste adaptada para resistir a estresses bióticos e abióticos relacionados a ambientes marinhos. Esta estrutura otimizada, que minimiza a energia e os materiais empregados, também pode representar um modelo ideal para a transferência de soluções funcionais bioinspiradas para construções de edifícios e diferentes setores industriais. A presente contribuição pretende fornecer uma breve visão geral sobre a adaptação morfofuncional estratégica equinoide do teste e seu potencial no campo biomimético

Clinical correlates of R1 relaxometry and magnetic susceptibility changes in multiple sclerosis: a multi-parameter quantitative MRI study of brain iron and myelin

Objectives: The clinical impact of brain microstructural abnormalities in multiple sclerosis (MS) remains elusive. We aimed to characterize the topography of longitudinal relaxation rate (R1) and quantitative susceptibility (χ) changes, as indices of iron and myelin, together with brain atrophy, and to clarify their contribution to cognitive and motor disability in MS. Methods: In this cross-sectional study, voxel-based morphometry, and voxel-based quantification analyses of R1 and χ maps were conducted in gray matter (GM) and white matter (WM) of 117 MS patients and 53 healthy controls. Voxel-wise between-group differences were assessed with nonparametric permutation tests, while correlations between MRI metrics and clinical variables (global disability, cognitive and motor performance) were assessed both globally and voxel-wise within clusters emerging from the between-group comparisons. Results: MS patients showed widespread R1 decrease associated with more limited modifications of χ, with atrophy mainly involving deep GM, posterior and infratentorial regions (p < 0.02). While R1 and χ showed a parallel reduction in several WM tracts (p < 0.001), reduced GM R1 values (p < 0.001) were associated with decreased thalamic χ (p < 0.001) and small clusters of increased χ in the caudate nucleus and prefrontal cortex (p < 0.02). In addition to the atrophy, χ values in the cingulum and corona radiata correlated with global disability and motor performance, while focal demyelination correlated with cognitive performance (p < 0.04). Conclusions: We confirmed the presence of widespread R1 changes, involving both GM and WM, and atrophy in MS, with less extensive modifications of tissue χ. While atrophy and χ changes are related to global and motor disability, R1 changes are meaningful correlates of cognition. Key points: • Compared to healthy controls, multiple sclerosis patients showed R1 and χ changes suggestive of iron increase within the basal ganglia and reduced iron and myelin content within (subnuclei of) the thalamus. • Thalamic volume and χ changes significantly predicted clinical disability, as well as pulvinar R1 and χ changes, independently from atrophy. • Atrophy-independent R1 and χ changes, suggestive of thalamic iron and myelin depletion, may represent a sensitive marker of subclinical inflammation