In vivo therapeutic efficacy of frog skin-derived peptides against Pseudomonas aeruginosa-induced pulmonary infection

Pseudomonas aeruginosa is an opportunistic and frequently drug-resistant pulmonary pathogen especially in cystic fibrosis sufferers. Recently, the frog skin-derived antimicrobial peptide (AMP) Esc(1-21) and its diastereomer Esc(1-21)-1c were found to possess potent in vitro antipseudomonal activity. Here, they were first shown to preserve the barrier integrity of airway epithelial cells better than the human AMP LL-37. Furthermore, Esc(1-21)-1c was more efficacious than Esc(1-21) and LL-37 in protecting host from pulmonary bacterial infection after a single intra-tracheal instillation at a very low dosage of 0.1 mg/kg. The protection was evidenced by 2-log reduction of lung bacterial burden and was accompanied by less leukocytes recruitment and attenuated inflammatory response. In addition, the diastereomer was more efficient in reducing the systemic dissemination of bacterial cells. Importantly, in contrast to what reported for other AMPs, the peptide was administered at 2 hours after bacterial challenge to better reflect the real life infectious conditions. To the best of our knowledge, this is also the first study investigating the effect of AMPs on airway-epithelia associated genes upon administration to infected lungs. Overall, our data highly support advanced preclinical studies for the development of Esc(1-21)-1c as an efficacious therapeutic alternative against pulmonary P. aeruginosa infection

La sexualidad humana “riqueza de toda la persona”

Para comprender plenamente el gran valor encerrado en la relación sexual-genital, debemos recordar que la relación, en la cual el hombre y la mujer se entregan recíprocamente, es también el lugar donde puede concretarse la llamada a la existencia de una nueva vida. De este modo el origen del ser humano viene a ser fruto y signo de la unión no sólo física sino también afectiva-espiritual de sus padres, los cuales deben sentir luego como derecho/deber el garantizar al hijo un crecimiento sereno en un contexto familiar estable

Exploring Returnee Migrant Women, COVID-19 and Sustainability in Spain

The COVID-19 pandemic has signified an historical change in human mobility. By transforming the patterns of people on the move, it has highlighted gender-based inequalities and women’s vulnerabilities. The link between COVID-19 and return migration shapes returnees’ readaptation process in their home countries, as returnees are embedded in a limbo between the pandemic’s pressure on the policy and socio-economic setting, on one hand, and their efforts for reintegration, on the other. Due to the pandemic, the gender-based imbalance has increased existing gender gaps both in migration and return, exacerbating women’s vulnerability. Thus, personal aspirations and professional expectations of highly educated women are caught in a system of socio-economic and geographical (im)mobility, which represents the principal outcome in their relocation and readaptation process. Based on a qualitative methodology through the analysis of ten life histories of highly educated returnee migrant women, this paper sheds light on the impacts of the COVID-19 pandemic on their migratory trajectories, providing a typology of them. Findings stress the necessity for more sustainable measures and resources for life–work balance and gender-sensitive policies, to promote a better integration process into the local labour market; to mitigate the effects of COVID-19 on returnee women, and to prevent the proliferation of mental health problems among returnee women

Effect of Enterococcus faecium SF68 on growth performance and in vivo digestibility in buffalo calves

The effect of dietary supplementation with Enterococcus faecium strain SF68 on growth performance, faecal consistency and in vivo digestibility in buffalo (Bubalus bubalis) calves was evaluated. Forty calves were randomly assigned at 10 d of age to one of four treatments: (A) milk replacer with no additive, (B) milk replacer supplemented with 0.17 g/l of viable (2 x l09 cfu/g) E. faecium bacteria daily for 3 days with an interval of 7 days throughout 11 weeks, (C) milk replacer supplemented with E. faecium daily for 4 weeks, (D) milk replacer supplemented with E. faecium daily for 11 weeks. A total mixed ration was offered ad libitum from 5th week of the experimental period. Faecal score was significantly better in E. faecium-treated calves than control ones. The use of E. faecium had no effect on average daily gain at any stage, total body weight (BW) gain, dry matter intake or total tract digestibility. Therefore, E. faecium supplementation may be able to act favourably on the health of the gastro-intestinal trac

Fatty acid composition of Mediterranean buffalo milk fat

The purpose of this research was to investigate the variation in fatty acid composition of milk fat from four buffalo (Bubalus bubalis) herds under different feeding management and ration composition. Changes in milk fatty acid composition were monitored on a weekly basis. Saturated fatty acids (65.5%) predominated in buffalo milk fat; monounsaturated and polyunsaturated fatty acids were 27.0% and 4.5%, respectively. Of saturated fatty acids, the content of palmitic acid was the highest (30.6%) followed by stearic acid (12.0%) and myristic acid (10.7%). Of the unsaturated fatty acids the content of oleic acid was the highest (26.6%). The average content of conjugated linoleic acid (0.76±0.33) was higher than the maximal values generally reported for dairy cow

Incommensurate phases of a bosonic two-leg ladder under a flux

A boson two--leg ladder in the presence of a synthetic magnetic flux is investigated by means of bosonization techniques and Density Matrix Renormalization Group (DMRG). We follow the quantum phase transition from the commensurate Meissner to the incommensurate vortex phase with increasing flux at different fillings. When the applied flux is $\rho \pi$ and close to it, where $\rho$ is the filling per rung, we find a second incommensuration in the vortex state that affects physical observables such as the momentum distribution, the rung-rung correlation function and the spin-spin and charge-charge static structure factors.Comment: 19 pages, 9 EPS figures, RevTeX 4 (v1); 20 pages, 10 EPS figures, improved section on mean-field theory (v2

Persisting Meissner state and incommensurate phases of hard-core boson ladders in a flux

The phase diagram of a half-filled hard core boson two-leg ladder in a flux is investigated by means of numerical simulations based on the Density Matrix Renormalization Group (DMRG) algorithm and bosonization. We calculate experimentally accessible observables such as the momentum distribution, as well as rung current, density wave and bond-order wave correlation functions, allowing us to identify the Mott Meissner and Mott Vortex states. We follow the transition from commensurate Meissner to incommensurate Vortex state at increasing interchain hopping till the critical value [Piraud et al. Phys. Rev. B v. 91, p. 140406 (2015)] above which the Meissner state is stable at any flux. For flux close to $\pi$, and below the critical hopping, we observe the formation of a second incommensuration in the Mott Vortex state that could be detectable in current experiments.Comment: RevTeX 4, 5 pages + 8 pages supplemental, 6 EPS figures; (v2) references added, corrected the discussion of the Meissner state at high interchain hoppin