Functional Crown Lengthening with Osteotomy in Anterior Teeth as a Prevention Against Restoration Failure: A Case Report

Background: Teeth with inadequate clinical crowns lead to failure in restorative treatment. This condition complicates the tooth isolation in root canal treatment and results in minimal retention. Functional crown lengthening is the most common surgical procedure performed to increase the length of the clinical crown. Case Report: This case report discusses the functional crown lengthening along with osteotomy on the anterior teeth of a 25-five-year-old male patient with a chief complaint of loose crown denture on the upper left lateral incisor accompanied with soreness after root canal treatment. Area #22 was seen as a gingival excess with a clinical crown remaining <2 mm on intraoral examination and showed non-hermetic obturation on radiographic examination. Conclusion: The effectiveness of the treatment was proven by the nonrecurrence of gingival excess as seen from a one-month follow-up after functional crown lengthening. Keywords: functional crown lengthening, osteotomy, gingival exces

Identificación de un aislamiento argentino de Spodoptera frugiperda granulovirus

La oruga militar tardía, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), es una plaga importante del maíz. Debido al impacto ambiental y a la aparición de resistencia causados por los pesticidas químicos y los eventos transgénicos, el uso de baculovirus resulta una alternativa útil y saludable para su control en estrategias de manejo integrado de plagas. En este trabajo reportamos la identificación de un nuevo aislamiento del granulovirus de la S. frugiperda nativo de la región central de Argentina, SfGV ARG. Se observó que larvas infectadas con SfGV ARG mostraron coloración amarillenta, hinchazón y, en algunos casos, lesiones graves en los últimos segmentos abdominales. Se confirmó la identidad del aislamiento por secuenciación de fragmentos de los genes lef-8, lef-9 y granulina, y por cálculo de distancias evolutivas usando el parámetro de Kimura-2. El patrón de restricción generado con el ADN genómico de SfGV ARG permitió estimar un tamaño de genoma de al menos 135 kb.The fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), is an important maize pest. Due to the environmental impact and emergence of resistance caused by chemical pesticides and transgenic events, the use of baculoviruses becomes a safe and useful alternative for its control in integrated pest management strategies. Here we report the identification of a novel isolate of a granulovirus of S. frugiperda native to the central region of Argentina, named SfGV ARG. We observed that larvae infected with SfGV ARG showed a yellowish coloration, swollen body and, in some cases, severe lesions in the last abdominal segments. We confirmed the identity of the isolate by sequencing fragments of the lef-8, lef-9 and granulin genes and by calculating evolutionary distances using the Kimura-2-Parameter model. SfGV ARG DNA restriction pattern allowed to estimate a genome of at least 135 kb.Fil: Pidre, Matias Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Sabalette, Karina Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Romanowski, Victor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Ferrelli, Maria Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentin

Histopathology report on colon cancer specimens; measuring surgical quality, an increasing stress for surgeons

Introduction. Improving the quality of surgical resections by evaluating surgical specimens is probably the most important feedback a surgeon can receive. Moreover, prognosis of patients with colon cancer is based on achieving appropriate resection margins and assessment of lymph node status. For these reasons we aim to provide a retrospective analysis on colon cancer specimens operated by a single surgical team. Materials and Methods. 88 patients operated between 2013 and 2016 were included in the study. Data were gathered prospectively and assessed by multivariate analysis for the main variables (age, gender, tumor staging, specimen length, distance to closest resection margin, number of lymph nodes, and number of positive lymph nodes). Results. The mean number of lymph nodes excised was 31,9, with more after right colectomies (39.6) than after left colonic resections (29.1). The average specimen length was 29.2cm after right colectomies, 35.6cm after left hemicolectomies and 18cm after segmental colectomies. There was a significant correlation between the number of lymph nodes, specimen length, and age of patients. Conclusion. Lymph node status is correlated with specimen length and age. The standard of 12 lymph nodes was achieved and surpassed, being comparable to the benchmark literature. Standards on colon resections need to be reevaluated as many surgeons are pressured by quality measurements which do not always reflect sound oncologic principles

REGENERATIVE TREATMENT USING PERIODONTAL FLAP SURGERY COMBINED Ti-OSS®, PERICARDIUM MEMBRANE AND PLATELET-RICH FIBRIN IN CHRONIC PERIODONTITIS

Introduction: Periodontal regenerative treatment with periodontal flap surgery followed by application of bone graft combined with platelet-rich fibrin (PRF) is a treatment using the concept of guided bone regeneration which is required to stimulate bone and periodontal tissue regeneration in chronic periodontitis with radiographic sign of alveolar bone vertical defect. This case report will describe the flap surgery with bone graft (Ti-OSS®, South Korea) and PRF treatment for correction of alveolar bone vertical defect in tooth with chronic periodontitis. Case: A 45-years old female complained of swollen and suppurated gingiva in the upper right area. Clinical finding showed deep periodontal pockets in tooth 13 and 15 and radiographic finding showed apical third alveolar bone loss in tooth 13 and 15. Case Treatment: Initial treatments included scaling and root planing, occlusal adjustment, and antibiotic prescription. Treatments were followed by periodontal flap surgery for debridement and elimination of granulation tissue on the root and alveolar bone surface of tooth 13 to 15, application of Ti-OSS® combined with PRF and pericardium membrane. Discussion: The follow-up evaluation 2 months after treatment showed clinical finding of decreased Clincial Attachment Level (CAL) and radiographic finding showed increased alveolar bone height around defect area of tooth 13 and 15. Conclusion and Suggestions: Periodontal flap surgery combined with application of Ti-OSS® and PRF may able to stimulate regeneration of alveolar bone and periodontal tissue in chronic periodontiti

Pelizaeus Merzbacher Disease: Dysmyelination Versus Demyelination

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Caloplaca subpallida (Teloschistaceae), a lichen species new to Poland: distribution, ecology and taxonomic affinities

Caloplaca subpallida is reported from basic and altered ultrabasic rocks (i.e. basalt, greenstone, and serpentinite) at nine sites in SW Poland. A detailed description of the species and a discussion on its taxonomic affinities are provided