Increased interleukin-1β levels following low dose MDMA induces tolerance against the 5-HT neurotoxicity produced by challenge MDMA

<p>Abstract</p> <p>Background</p> <p>Preconditioning is a phenomenon by which tolerance develops to injury by previous exposure to a stressor of mild severity. Previous studies have shown that single or repeated low dose MDMA can attenuate 5-HT transporter loss produced by a subsequent neurotoxic dose of the drug. We have explored the mechanism of delayed preconditioning by low dose MDMA.</p> <p>Methods</p> <p>Male Dark Agouti rats were given low dose MDMA (3 mg/kg, i.p.) 96 h before receiving neurotoxic MDMA (12.5 mg/kg, i.p.). IL-1β and IL1ra levels and 5-HT transporter density in frontal cortex were quantified at 1 h, 3 h or 7 days. IL-1β, IL-1ra and IL-1RI were determined between 3 h and 96 h after low dose MDMA. sIL-1RI combined with low dose MDMA or IL-1β were given 96 h before neurotoxic MDMA and toxicity assessed 7 days later.</p> <p>Results</p> <p>Pretreatment with low dose MDMA attenuated both the 5-HT transporter loss and elevated IL-1β levels induced by neurotoxic MDMA while producing an increase in IL-1ra levels. Low dose MDMA produced an increase in IL-1β at 3 h and in IL-1ra at 96 h. sIL-1RI expression was also increased after low dose MDMA. Coadministration of sIL-1RI (3 μg, i.c.v.) prevented the protection against neurotoxic MDMA provided by low dose MDMA. Furthermore, IL-1β (2.5 pg, intracortical) given 96 h before neurotoxic MDMA protected against the 5-HT neurotoxicity produced by the drug, thus mimicking preconditioning.</p> <p>Conclusions</p> <p>These results suggest that IL-1β plays an important role in the development of delayed preconditioning by low dose MDMA.</p

Association Between Maxillary Sinus Pathologies And Healthy Teeth

The proximity of the roots to the maxillary sinus can create a variety of risks. Objective: To evaluate the relationship between the roots of healthy teeth and the maxillary sinus, as well as the occurrence of sinus pathologies. Methods: Three radiologists analyzed 109 cone beam computed tomography (CBCT) images. The Kappa test was used to assess the intra- and inter-rater agreement. The chi-squared test and prevalence ratio were used to test the hypothesis that roots of healthy teeth in the maxillary sinus favored the occurrence of sinus pathologies (p = 0.01). Results: Intra- and inter-rater agreement ranged from good to excellent. The chi-squared test demonstrated a statistically significant difference (p= 0.006) between the tooth roots in diseased maxillary sinuses (6.09%) and those in normal sinuses (3.43%). The prevalence ratio test showed a statistically significant higher prevalence of tooth roots in diseased sinuses than in normal sinuses (p < 0.0001). Roots in the maxillary sinus were 1.82 times more associated with diseased sinuses. Conclusion: Dental roots in the maxillary sinus are almost twice as likely to be associated with diseased sinuses than normal sinuses. Healthy teeth whose roots are inside the maxillary sinus may induce an inflammatory response in the sinus membrane. It is suspected that dental procedures may exacerbate the condition. (C) 2015 Associacao Brasileira de Otorrinolaringologia e Cirurgia Cervico-Facial. Published by Elsevier Editora Ltda. All rights reserved.821333

Length-weight relationships for 22 crustaceans and cephalopods from the Gulf of Cadiz (SW Spain)

Life history traits are available for many fish species in different regions, but less so for invertebrates such as cephalopods and crustaceans, though, they are increasingly needed for implementing an ecosystem-based approach. Recent food web modelling in the Gulf of Cadiz has identified invertebrates as keystone groups. However, information on life history traits of such groups remains incomplete in this region. To fill this knowledge gap, we report length-weight relationships for 12 cephalopods and 10 crustaceans collected in the Gulf of Cadiz from 2009 to 2013. This study reports, for the first time, life history traits of nine species in the area (Chlorotocus crassicornis, Pasiphaea sivado, Plesionika heterocarpus, Plesionika martia, Processa canaliculata, Solenocera membranacea, Allotheutis media, Sepia orbignyana and Sepietta oweniana). For each species, length-weight relationships, minimum and maximum lengths, mean weights, and depth ranges are presented. Overall, the results revealed that all species showed negative allometric growth (hypoallometry), except P. sivado, the only species showing an isometric growth pattern. We expect that this study will contribute to link sustainable fisheries with biodiversity conservation goals enabling the implementation of operational ecosystem-based management in the Gulf of Cadiz.IEO through FPIinfo:eu-repo/semantics/publishedVersio

Descoloration of industrial dyes and simulated textile effluents dyes by turnip peroxidase

The removal of important textile dyes by turnip peroxidase (TNP) was evaluated. The textile effluents besides the residual dyes contain also chemical auxiliaries such as salts, dispersing and wetting agents. The effect of these was evaluated in the removal of the dyes reactive blue 21 and reactive blue 19 by TNP in synthetic effluents. A decrease of the efficency decolorization was observed. The action of the enzyme on colour removal of dye mixture was equivalent to the dyes alone. The chemical demand of oxygen in the effluent after enzymatic treatment had a significant increase in relation to the untreated effluent

Multi-temporal unmixing analysis of Hyperion images over the Guanica Dry Forest

This paper presents a methodology to analyze time-series data from Hyperion to study seasonal vegetation dynamics on the Guánica Dry Forest in Puerto Rico. Unmixing analysis is performed over ten near-cloud-free Hyperion images collected in different months in 2008. Abundance maps and endmembers estimated from the unmixing procedure are used to analyze the seasonal changes in the forest. Results from the analysis are compared with published knowledge of the Guanica Forest phenology

Population genetic analysis and trichothecene profiling of Fusarium graminearum from wheat in Uruguay

Fusarium graminearum sensu stricto (F. graminearum s.s.) is the major causal agent of Fusarium head blight of wheat worldwide, and contaminates grains with trichothecene mycotoxins that cause serious threats to food safety and animal health. An important aspect of managing this pathogen and reducing mycotoxin contamination of wheat is knowledge regarding its population genetics. Therefore, isolates of F. graminearum s.s. from the major wheat-growing region of Uruguay were analyzed by amplified fragment length polymorphism assays, PCR genotyping, and chemical analysis of trichothecene production. Of the 102 isolates identified as having the 15-ADON genotype via PCR genotyping, all were DON producers, but only 41 strains were also 15-ADON producers, as determined by chemical analysis. The populations were genotypically diverse but genetically similar, with significant genetic exchange occurring between them. Analysis of molecular variance indicated that most of the genetic variability resulted from differences between isolates within populations. Multilocus linkage disequilibrium analysis suggested that the isolates had a panmictic population genetic structure and that there is significant recombination occurs in F. graminearum s.s. In conclusion, tour findings provide the first detailed description of the genetic structure and trichothecene production of populations of F. graminearum s.s. from Uruguay, and expands our understanding of the agroecology of F. graminearum and of the correlation between genotypes and trichothecene chemotypes.Fil: Pan, D.. Universidad de la República; UruguayFil: Mionetto, A.. Universidad de la República; UruguayFil: Calero, N.. Universidad de la República; UruguayFil: Reynoso, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Torres, Adriana Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Bettucci, L.. Universidad de la República; Urugua

Obtention of plant peroxidase and its potential for the decolorization of the reactive dye Remazol Turquoise G 133%

Peroxidases can be used in the decolorization process. There is a growing interest for new sources of this enzyme and for obtaining economically viable processes. In this work, a low-cost vegetable peroxidase extraction process is proposed; the resulting enzyme is characterized to determine its optimum pH, temperature, and stability conditions, and it is then applied in the decolorization of reactive dye Remazol Turquoise G 133%. The turnip peroxidase (TP) was utilized as an enzymatic source. This enzyme exhibited maximum activity at pH 7.0, and it was active in the temperature range of 30 to 50 °C, which favors its use in industrial processes. Acetone was the most efficient solvent to induce precipitation. The removal of Remazol Turquoise G 133% was 56.0% complete after 50 min, while 41.0% of the same dye was removed with the commercial horseradish peroxidase enzyme in 50 min. TP presents potential as a viable alternative in the decolorization of textile wastewaters

Anthracnose: The sophisticated rot

The mold fungus Colletotrichum graminicola causes anthracnose, one of the most economically damaging corn diseases worldwide. Anthracnose can occur either as a stalk rot (ASR), or a leaf blight (ALB) (4; 27). The leaf blight phase is generally insignificant in North America as a cause of yield loss, although in the tropics and subtropics it is much more important. Resistance to ASR is usually not correlated with resistance to ALB, complicating efforts to breed resistant corn varieties (2; 4). Resistance to ASR and ALB is mostly quantitative, although sources of major gene resistance have been described (10; 29). Hybrids containing some of these major-gene resistance sources are likely to become available for management of ASR in the near future

Asymptotic properties of parameter estimates for random fields with tapered data

In this paper we present novel results on the asymptotic be-havior of the so-called Ibragimov minimum contrast estimates. The case of tapered data for various models of Gaussian random fields is investigated. MSC 2010 subject classifications: Primary 62F12, 62M30; secondary 60G60