Studies on s-allele incompatibility in brassica oleracea

By the end of 1972, a total of thirteen rabbits had been injected. One serum was raised to Brassica oleracea S23S23 kale pollen proteins, but no s-antibody was stimulated. All the others were raised to stigma proteins. A range of s-alleles and varieties of B.oleracea was used including S23S23, S16S16 kale, S2S2, S45S45 and S5S5 brussels sprout and S15S15 cabbage. S15 and S5 were of low dominance, S2 and S45 of intermediate dominance and S23, S14 and S16 of high dominance. S-antibodies were raised to S23 and S16 in kale, and S2 and S45 in brussels sprout. Only the S16-antibody had a high titre of 1/16, the other were 1/4 or less. In two of the cases , booster injections stimulated the S-antibody response where the first course of injections stimulated the S-antibody response where the first course of injections stimulated the S-antibody response where the first course of injections had failed. Relatively dilute stigma extracts of 250 stigmas/ml stimulated most s-antibodies. An injection schedule of more than two months produced cross-reactions or new specificities. Extract which had been frozen or treated with formalin gave a poorer antibody response than freshly-prepared extract. No S-antibodies were stimulated by these treatments. In its present form, the technique was unsuitable for routine S-allele diagnosis in B.oleracea because of the low rate of successful sera and the low titres stimulated. Improvements to the technique are suggested. Between 35 and 40% of the total protein content was lost when a stigma extract was frozen, but the S-protein was not lost. B.oleracea pollen germinated on an agar and sucrose medium. Germination was stimulated by 0.0005% queroetin, but tube growth was not. Querotin was detected as glycosides in the pollen and stigma tissue of B.oleracea. It was not involved in the incompatibility reaction of the stigma

Hybridisation Techniques for Acacias

Crop Production/Industries,

Construction of an almond linkage map in an Australian population Nonpareil × Lauranne

Background: Despite a high genetic similarity to peach, almonds (Prunus dulcis) have a fleshless fruit and edible kernel, produced as a crop for human consumption. While the release of peach genome v1.0 provides an excellent opportunity for almond genetic and genomic studies, well-assessed segregating populations and the respective saturated genetic linkage maps lay the foundation for such studies to be completed in almond. Results: Using an almond intraspecific cross between ‘Nonpareil’ and ‘Lauranne’ (N × L), we constructed a moderately saturated map with SSRs, SNPs, ISSRs and RAPDs. The N × L map covered 591.4 cM of the genome with 157 loci. The average marker distance of the map was 4.0 cM. The map displayed high synteny and colinearity with the Prunus T × E reference map in all eight linkage groups (G1-G8). The positions of 14 mapped gene-anchored SNPs corresponded approximately with the positions of homologous sequences in the peach genome v1.0. Analysis of Mendelian segregation ratios showed that 17.9% of markers had significantly skewed genotype ratios at the level of P < 0.05. Due to the large number of skewed markers in the linkage group 7, the potential existence of deleterious gene(s) was assessed in the group. Integrated maps produced by two different mapping methods using JoinMap® 3 were compared, and their high degree of similarity was evident despite the positional inconsistency of a few markers. Conclusions: We presented a moderately saturated Australian almond map, which is highly syntenic and collinear with the Prunus reference map and peach genome V1.0. Therefore, the well-assessed almond population reported here can be used to investigate the traits of interest under Australian growing conditions, and provides more information on the almond genome for the international community.Iraj Tavassolian, Gholmereza Rabiei, Davina Gregory, Mourad Mnejja, Michelle G Wirthensohn, Peter W Hunt, John P Gibson, Christopher M Ford, Margaret Sedgley, and Shu-Biao W