Symptoms accompanying fibromyalgia

Fibromyalgia is a pain syndrome frequently observed in clinical practice. The classification criteria for fibromyalgia were proposed by Wolfe et al. (1), who clearly indicated the essential clinical features of the syndrome, i.e. chronic widespread pain and muscular tender points in at least 11 out of 18 sites of localisation. It must be noted that the criteria of Wolfe et al. were proposed for epidemiological and research purposes, but are currently used as diagnostic criteria. In patients suffering from fibromyalgia, the intensity of spontaneous pain and of pain provoked by mechanical stimulation of tender points may vary in a wide range and may induce more or less suffering and disability. Therefore, the intensity of spontaneous and provoked pain must be taken into account to evaluate the severity of the syndrome in every patient..

Highly conductive, ionic liquid-based polymer electrolytes

In this manuscript is reported a thermal and impedance spectroscopy investigation carried out on quaternary polymer electrolytes, to be addressed as separators for lithium solid polymer batteries, containing large amount of the N-methyl-N-propylpyrrolidinium bis(fluorosulfonyl)imide ionic liquid. The target is the development of Li+ conducting membranes with enhanced ion transport even below room temperature. Polyethylene oxide and polymethyl methacrylate were selected as the polymeric hosts. A fully dry, solvent-free procedure was followed for the preparation of the polymer electrolytes, which were seen to be self-consistent and handled even upon prolonged storage periods (more than 1 year). Appealing ionic conductivities were observed especially for the PEO electrolytes, i.e., 1.6 × 10-3and 1.5 × 10-4 S cm-1 were reached at 20 and -20°C, respectively, which are ones the best, if not the best ion conduction, never detected for polymer electrolytes

The condensin complex is required for proper spindle assembly and chromosome segregation in Xenopus egg extracts.

Chromosome condensation is required for the physical resolution and segregation of sister chromatids during cell division, but the precise role of higher order chromatin structure in mitotic chromosome functions is unclear. Here, we address the role of the major condensation machinery, the condensin complex, in spindle assembly and function in Xenopus laevis egg extracts. Immunodepletion of condensin inhibited microtubule growth and organization around chromosomes, reducing the percentage of sperm nuclei capable of forming spindles, and causing dramatic defects in anaphase chromosome segregation. Although the motor CENP-E was recruited to kinetochores pulled poleward during anaphase, the disorganized chromosome mass was not resolved. Inhibition of condensin function during anaphase also inhibited chromosome segregation, indicating its continuous requirement. Spindle assembly around DNA-coated beads in the absence of kinetochores was also impaired upon condensin inhibition. These results support an important role for condensin in establishing chromosomal architecture necessary for proper spindle assembly and chromosome segregation

A minor alternative transcript of the fumarylacetoacetate hydrolase gene produces a protein despite being likely subjected to nonsense-mediated mRNA decay

BACKGROUND: Coupling of alternative splicing with nonsense-mediated mRNA decay (NMD) may regulate gene expression. We report here the identification of a nonsense alternative transcript of the fumarylacetoacetate hydrolase (fah) gene, which produces a protein despite the fact that it is subject to NMD. RESULTS: During the characterization of the effects of the W262X nonsense mutation on FAH mRNA metabolism, two alternative transcripts (del100 and del231) of the fah gene were identified. Del100 lacks exon 8 and as a consequence, the reading frame is shifted and a premature termination codon appears at the 3'end of exon 10. Exons 8 and 9 are skipped in del231, without any disruption of the reading frame. Specific amplification of these transcripts demonstrate that they are produced through minor alternative splicing pathways, and that they are not caused by the W262X mutation per se. As shown with an antiserum raised against the C-terminal part of the putative DEL100 protein, the del100 transcript produces a protein, expressed at different levels in various human tissues. Interestingly, the del100 transcript seems to be subjected to nonsense-mediated mRNA decay, as its level was stabilized following a cycloheximide treatment. CONCLUSIONS: The del100 and del231 transcripts arise due to minor alternative splicing pathways and del100 is likely subjected to nonsense-mediated mRNA decay. However the remaining amount of transcript seems sufficient to produce a protein in different human tissues. This suggests that NMD has a broader role than simply eliminating aberrant transcripts and when coupled to alternative splicing, may act to modulate gene expression, by allowing the production of low amounts of protein

Compliance and medication problems in chronic conditions

Objectives: The project aimed to determine the level of medication compliance in patients having a chronic condition and to determine the types and frequency of medication-related problems identified by a pharmacist working in a community pharmacy. Method: The study population was identified from the database of patients receiving free medicinals under the ‘Pharmacy of your Choice’ scheme from a particular community pharmacy. Patients included in the study were older than 60 years of age and taking more than 3 drugs. A Compliance Questionnaire was distributed to these patients. The pharmacist classified medication problems and used a specifically developed data collection tool in order to collate all the relevant patient information. Key findings: Of the 75 patients included in the study, 33 (44%) said they never missed a dose whilst the remaining 42 (56%) were non-compliant. Out of the 205 medication-related problems identified, the most common medication-related problems observed were non-compliance (56%) and the occurence of adverse drug reactions (55%). Conclusion: Compliance is a significant medication-related problem encountered by community pharmacists amongst elderly patients receiving multiple drug therapy for their chronic conditions.peer-reviewe

Excitations of torelon

The excitations of gluonic flux tube in a periodic lattice are examined. Monte Carlo simulations from an anisotropic lattice are presented and the comparison with effective string models is discussed.Comment: Talk at Lattice 2003; 3 pages, 4 figure

Development of a Calibration Model for Optical Measuring Machines

AbstractThis paper presents the problem of optical measuring machine calibration, emphasizing the calibration of the “optical system”, omitting the calibration of the “machine system”. The calibration of an optical measuring machine is the first step before using the instrument for any application. For this purpose, a mathematical model has been developed to transform the coordinates of a point in space (3D) into coordinates of a point in an image (2D). Using this camera model, a calibration procedure has been developed using a grid distortion pattern. Finally, a procedure for calculating the uncertainty of the camera and geometric distortion parameters based on the Monte Carlo method has been developed

Effect of Lupinus albus as protein supplement on yield, constituents, clotting properties and fatty acid composition in ewes' milk

The effect of feeding lupin seeds (Lupinus albus L.) as an alternative protein source in ewe diets was investigated. Two groups of 18 Sarda ewes were fed two different isonitrogenous diets: with lupin (L) seed, given after 12 h soaking, or soybean meal (SBM) as the main protein source. DMI, variations of body weight and milk production were unaffected by the treatment. Although not statistically significant, in the group fed L diet the production of milk fat and protein was higher. Clotting properties of milk were similar for the two treatments, probably due to the small differences in the milk protein contents. The fatty acid profile of milk was affected by treatment with a larger content of short (14.19 wt% versus 12.26 wt%)- and medium (49.37 wt% versus 47.76 wt%)-chain fatty acids in milk from ewes fed the L diet. CLA content was unaffected by treatment. Triglyceride content of fat from the two diets reflects the milk fatty acid composition. Indeed, milk from L diet showed a higher level of medium-chain triglycerides, which are of particular interest to consumers with concerns over health and heart disease. The inclusion of lupin seed in the diet of lactation ewes can be a means of achieving a more desirable triglyceride profile in milk fat. Milk with enhanced nutritive quality may promote wider market penetration of sheep dairy products

Resistance to apramycin of Salmonella and E.coli isolated from swine

The aim of this study was to determine the prevalence of aminoglycosides antibiotic resistance in Salmonella spp. and E. coli strains. 32 E. coli, and 47 Salmonella spp., isolated from cases of enteritis in growers and fatteners from 1998 to 2002 in Umbria and Marche regions, were tested. Susceptibility to gentamicin, tobramycin and streptomycin was determined by Kirby-Bauer method, apramycin by microdilution method. 92,4 % of the strains tested were susceptible to apramycin, 77,2 % to gentamicin, 67,1 % to tobramycin and 35,4 % to streptomycin. A positive statistical association between gentamicin and apramycin (RR = 7,63; p = 0,014), tobramycin and apramycin (RR = 9,22; p = 0,027) was demonstrated. There is no difference between the association apramycin-streptomycin, suggesting a mechanism of resistance related to the presence of the aminoglycoside acetyltranspherase IV enzyme. The trend based on estimated OR from the resistance of the strains for every year considered was significant (p = 0,00049), showing a progressive decrease from 1998 (OR = 1) to 2002 (OR = 0,3)

A 0-dimensional counter-example to rooting?

We provide an example of a 0-dimensional field theory where rooting does not work.Comment: 3 pages; Physics Letters B (2010