12 research outputs found
Vergleich und Validierung aberranter DNA-Methylierungsmuster in unterschiedlichen LungenkarzinomentitÀten
Aberrante DNA-Methylierungsmuster sind ein charakteristischer Befund in zahlreichen Tumoren. Diese im Laufe des Lebens erworbenen Alterationen können maĂgeblich zu der Entstehung von Krebs beitragen.
Im Rahmen der Forschung des Deutschen Zentrums fĂŒr Lungenkrankheiten (DZL) wurden in einem Array-basierten Ansatz aberrant methylierte DNA-Regionen in Lungenkarzinomen beobachtet.
Ziel dieser Arbeit ist die Identifizierung und Validierung einzelner Loci und Methylierungsmuster. Besonderes Augenmerk soll dabei auf der Differenzierung spezifischer Muster der verschiedenen TumorentitĂ€ten liegen, insbesondere im Hinblick auf das Adeno- und Plattenepithelkarzinom der Lunge. In einem neuen Ansatz werden in diesem Sinne OP-PrĂ€parate und insbesondere Paired Biopsies mithilfe der Bisulfit-Pyroseqenzierung analysiert. ZunĂ€chst wird hierfĂŒr mittels eines Software-unterstĂŒtzten Designs ein Assay etabliert, der im Folgenden an einer Zelllinie optimiert wird. Daran schlieĂt sich die Analyse der PrimĂ€rproben inklusive ihrer Auswertung an.
Die Diagnose des Lungenkarzinoms wird oftmals schon in einem schon weit fortgeschritten Stadium gestellt. Die therapeutischen Optionen werden zudem stadiengerecht und entsprechend der TumorentitÀt erwogen. Möglicherweise können die Ergebnisse dieser Arbeit in diesem Zusammenhang sowohl diagnostisch, als auch prognostisch wertvolle Marker darstellen
Soziale Arbeit als kritische Handlungswissenschaft : BeitrÀge zur (Re-)Politisierung Sozialer Arbeit
Soziale Arbeit ist politisch. Von diesem grundlegenden Sachverhalt ausgehend, entwickelt der Sammelband Perspektiven fĂŒr eine professionelle und wissenschaftliche Selbstbestimmung. Renommierte Sozialarbeitswissenschaftler/innen stellen Modelle einer Sozialen Arbeit als kritischer Handlungswissenschaft vor. Konstitutiv fĂŒr diese sind die Leid- und Unrechtserfahrungen, die Menschen in ihrem alltĂ€glichen Leben machen, und das emanzipatorische Interesse an gerechteren und freieren gesellschaftlichen VerhĂ€ltnissen.
Die BeitrĂ€ge des Bandes lassen sich als EinfĂŒhrungen in ein kritisches VerstĂ€ndnis von Sozialer Arbeit lesen. DarĂŒber hinaus dokumentieren sie die Vielfalt von Positionen im Diskurs ĂŒber eine zeitgemĂ€Ăe, demokratische und reflexive Soziale Arbeit. Grundlinien kritischer Sozialer Arbeit werden ebenso deutlich wie Differenzen, Kontroversen und offene Fragen. Der Band spricht Praktiker/innen, Wissenschaftler/innen wie Studierende der Sozialen Arbeit gleichermaĂen a
Additional file 2 of Outpatient follow-up of tumour diseases through video-based value-oriented behavioural activation (ViVA): study protocol for a randomised controlled trial
Additional file 2. Informed consent materials (original version)
Additional file 1 of Outpatient follow-up of tumour diseases through video-based value-oriented behavioural activation (ViVA): study protocol for a randomised controlled trial
Additional file 1. SPIRIT Checklist
Additional file 3 of Outpatient follow-up of tumour diseases through video-based value-oriented behavioural activation (ViVA): study protocol for a randomised controlled trial
Additional file 3. Informed consent materials (English version)
Safety of a compression stocking for patients with chronic venous insufficiency (CVI) and peripheral artery disease (PAD)
With increasing age, it is increasingly common for patients to develop both chronic venous insufficiency (CVI) and peripheral artery disease (PAD). While there are special compression bandage systems commercially available for individuals thus affected, appropriate compression stockings have previously not been available. In the present study, we investigated the safety and effectiveness of a type of compression stocking specifically designed for this patient group (VenoTrainÂź angioflow, Bauerfeind Germany, German compression class 1 with high stiffness).
In a prospective case series, we included patients with both CVI (CâC disease according to CEAP classification) and PAD (ankleâbrachial index of 0.5; absolute ankle systolic pressure of > 60 mmHg). Primary outcome measures consisted of 1) safety in terms of PAD, as determined by measuring acral pressure using acral photoplethysmography (APPG), and 2) effectiveness in terms of CVI symptoms, as assessed by using a suitable questionnaire (VVSymQ).
Fifty patients were evaluated (mean age: 67.1; mean ankleâbrachial index: 0.75 0.77). Fifteen patients had stage IIa PAD (according to Fontaine); 15, stage IIb; the remainder, stage I disease. Thirtyâone patients had stage C CVI (according to CEAP classification); 16 patients, stage C; and three patients, stage C disease. Immediately after donning the medical compression stocking, systolic arterial pressure in the big toe increased significantly (from 83.3 mmHg 27.6 mmHg to 90.8 mmHg 24.1 mmHg) (p = 0.026). The VVSymQ score dropped significantly from 5.0 4.95 points to 1.4 2.26 points (p < 0.001), thus reflecting an improvement in CVI symptoms.
The compression stocking tested herein is safe for individuals with an ankle brachial index â„ 0.5. Skin damage was not observed
Additional file 1: of Effectiveness and patient safety of platelet aggregation inhibitors in the prevention of cardiovascular disease and ischemic stroke in older adults â a systematic review
Search string search 1 and 2. (DOCX 102Â kb
DNA methylation profiles of bronchoscopic biopsies for the diagnosis of lung cancer
Background!#!Lung cancer is the leading cause of cancer-related death in most western countries in both, males and females, accounting for roughly 20-25% of all cancer deaths. For choosing the most appropriate therapy regimen a definite diagnosis is a prerequisite. However, histological characterization of bronchoscopic biopsies particularly with low tumor cell content is often challenging. Therefore, this study aims at (a) determining the value of DNA methylation analysis applied to specimens obtained by bronchoscopic biopsy for the diagnosis of lung cancer and (b) at comparing aberrantly CpG loci identified in bronchoscopic biopsy with those identified by analyzing surgical specimens.!##!Results!#!We report the HumanMethylation450-based DNA methylation analysis of paired samples of bronchoscopic biopsy specimens either from the tumor side or from the contralateral tumor-free bronchus in 37 patients with definite lung cancer diagnosis and 18 patients with suspicious diagnosis. A differential DNA methylation analysis between both biopsy sites of patients with definite diagnosis identified 1303 loci. Even those samples were separated by the set of 1303 loci in which histopathological analysis could not unambiguously define the dignity. Further differential DNA methylation analyses distinguished between SCLC and NSCLC. We validated our results in an independent cohort of 40 primary lung cancers obtained by open surgical resection and their corresponding controls from the same patient as well as in publically available DNA methylation data from a TCGA cohort which could also be classified with high accuracy.!##!Conclusions!#!Considering that the prognosis correlates with tumor stage at time of diagnosis, early detection of lung cancer is vital and DNA methylation analysis might add valuable information to reliably characterize lung cancer even in histologically ambiguous sample material