Robustness of force and stress inference in an epithelial tissue

During morphogenesis, the shape of a tissue emerges from collective cellular behaviors, which are in part regulated by mechanical and biochemical interactions between cells. Quantification of force and stress is therefore necessary to analyze the mechanisms controlling tissue morphogenesis. Recently, a mechanical measurement method based on force inference from cell shapes and connectivity has been developed. It is non-invasive, and can provide space-time maps of force and stress within an epithelial tissue, up to prefactors. We previously performed a comparative study of three force-inference methods, which differ in their approach of treating indefiniteness in an inverse problem between cell shapes and forces. In the present study, to further validate and compare the three force inference methods, we tested their robustness by measuring temporal fluctuation of estimated forces. Quantitative data of cell-level dynamics in a developing tissue suggests that variation of forces and stress will remain small within a short period of time ($\sim$minutes). Here, we showed that cell-junction tensions and global stress inferred by the Bayesian force inference method varied less with time than those inferred by the method that estimates only tension. In contrast, the amplitude of temporal fluctuations of estimated cell pressures differs less between different methods. Altogether, the present study strengthens the validity and robustness of the Bayesian force-inference method.Comment: 4 pages, 4 figure

Magnetic order in the Ising model with parallel dynamics

It is discussed how the equilibrium properties of the Ising model are described by an Hamiltonian with an antiferromagnetic low temperature behavior if only an heat bath dynamics, with the characteristics of a Probabilistic Cellular Automaton, is assumed to determine the temporal evolution of the system.Comment: 9 pages, 3 figure

Polarity patterns of stress fibers

Stress fibers are contractile actomyosin bundles commonly observed in the cytoskeleton of metazoan cells. The spatial profile of the polarity of actin filaments inside contractile actomyosin bundles is either monotonic (graded) or periodic (alternating). In the framework of linear irreversible thermodynamics, we write the constitutive equations for a polar, active, elastic one-dimensional medium. An analysis of the resulting equations for the dynamics of polarity shows that the transition from graded to alternating polarity patterns is a nonequilibrium Lifshitz point. Active contractility is a necessary condition for the emergence of sarcomeric, alternating polarity patterns.Comment: 5 pages, 3 figure

Gyromagnetic ratio of rapidly rotating compact stars in general relativity

We numerically calculate equilibrium configurations of uniformly rotating and charged neutron stars, in the case of insulating material and neglecting the electromagnetic forces acting on the equilibrium of the fluid. This allows us to study the behaviour of the gyromagnetic ratio for those objects, when varying rotation rate and equation of state for the matter. Under the assumption of low charge and incompressible fluid, we find that the gyromagnetic ratio is directly proportional to the compaction parameter M/R of the star, and very little dependent on its angular velocity. Nevertheless, it seems impossible to have g=2 for these models with low charge-to-mass ratio, where matter consists of a perfect fluid and where the collapse limit is never reached.Comment: 11 pages, 6 figures, accepted for publication in Classical and Quantum Gravit

Contraction of cross-linked actomyosin bundles

Cross-linked actomyosin bundles retract when severed in vivo by laser ablation, or when isolated from the cell and micromanipulated in vitro in the presence of ATP. We identify the time scale for contraction as a viscoelastic time tau, where the viscosity is due to (internal) protein friction. We obtain an estimate of the order of magnitude of the contraction time tau ~ 10-100 s, consistent with available experimental data for circumferential microfilament bundles and stress fibers. Our results are supported by an exactly solvable, hydrodynamic model of a retracting bundle as a cylinder of isotropic, active matter, from which the order of magnitude of the active stress is estimated.Comment: To be published in Physical Biolog

Detection and construction of an elliptic solution to the complex cubic-quintic Ginzburg-Landau equation

In evolution equations for a complex amplitude, the phase obeys a much more intricate equation than the amplitude. Nevertheless, general methods should be applicable to both variables. On the example of the traveling wave reduction of the complex cubic-quintic Ginzburg-Landau equation (CGL5), we explain how to overcome the difficulties arising in two such methods: (i) the criterium that the sum of residues of an elliptic solution should be zero, (ii) the construction of a first order differential equation admitting the given equation as a differential consequence (subequation method).Comment: 12 pages, no figure, to appear, Theoretical and Mathematical Physic

Nonlinear oscillator with parametric colored noise: some analytical results

The asymptotic behavior of a nonlinear oscillator subject to a multiplicative Ornstein-Uhlenbeck noise is investigated. When the dynamics is expressed in terms of energy-angle coordinates, it is observed that the angle is a fast variable as compared to the energy. Thus, an effective stochastic dynamics for the energy can be derived if the angular variable is averaged out. However, the standard elimination procedure, performed earlier for a Gaussian white noise, fails when the noise is colored because of correlations between the noise and the fast angular variable. We develop here a specific averaging scheme that retains these correlations. This allows us to calculate the probability distribution function (P.D.F.) of the system and to derive the behavior of physical observables in the long time limit

Synchronization in coupled map lattices as an interface depinning

We study an SOS model whose dynamics is inspired by recent studies of the synchronization transition in coupled map lattices (CML). The synchronization of CML is thus related with a depinning of interface from a binding wall. Critical behaviour of our SOS model depends on a specific form of binding (i.e., transition rates of the dynamics). For an exponentially decaying binding the depinning belongs to the directed percolation universality class. Other types of depinning, including the one with a line of critical points, are observed for a power-law binding.Comment: 4 pages, Phys.Rev.E (in press

Dynamic Simulations of the Kosterlitz-Thouless Phase Transition

Based on the short-time dynamic scaling form, a novel dynamic approach is proposed to tackle numerically the Kosterlitz-Thouless phase transition. Taking the two-dimensional XY model as an example, the exponential divergence of the spatial correlation length, the transition temperature $T_{KT}$ and all critical exponents are computed. Compared with Monte Carlo simulations in equilibrium, we obtain data at temperatures nearer to $T_{KT}$.Comment: to appear in Phys. Rev. E in Rapid Communicatio