Síndrome cerebelar por vacuolización neuronal y degeneración espinocerebelar en Rottweiler : primer caso descrito en España

Se presenta un caso clínico de síndrome cerebelar en un Rottweiler hembra joven

Efectos de la adición de compuestos fotoprotectores en la dieta del langostino Pleoticus muelleri

El langostino Pleoticus muelleri y el camarón Artemesia longinaris son especies de interés comercial distribuidas en la región sudoeste del Océano Atlántico, desde el sur de Brasil hasta la Patagonia (23-50 ºS). Durante las pasadas dos décadas, se ha evidenciado un incremento de la radiación ultravioleta (RUV) y particularmente la UVB (280-315nm) sobre la superficie terrestre. Si bien los camarones penaeoideos son especies costeras que habitan en general a profundidades mayores a los 10m, bajo condiciones de cultivo se mantienen en estanques de 2 metros de profundidad, por lo que están expuestos a condiciones ambientales extremas. Existe evidencia que en algunos animales los compuestos que absorben RUV se bioacumulan a través de la dieta, ofreciendo protección no sólo a sus productores sino también a los consumidores a través de la cadena trófica (Helbling et al., 2002).La mayoría de los estudios experimentales sobre el efecto de la RUV sobre organismos marinos se realizó sobre el zooplancton, focalizándose en pequeños animales como los copépodos o larvas de crustáceos (Marcoval et al., 2013) utilizándose la mortalidad como una medida de su efecto. El objetivo del presente trabajo fue determinar la bioacumulación de compuestos fotoprotectores a partir de una dieta adicionada con harina de algas rojas en juveniles de P.muelleri y su posible efecto protector bajo condiciones de stress por radiación ultravioleta (RUV)

Caracterización de sistemas de localización en Android

El objetivo de este proyecto se basa en la caracterización de la tecnología Geofence como un sistema de localización en Android. Como no hay información real del comportamiento de este sistema, se da un primer paso a su experimentación ante diferentes escenarios, para ser capaces de entender cómo actúa Geofence en la práctica, y además de eso, implementar un algoritmo que sea capaz de rastrear una persona con un teléfono móvil.The goal of this project is based on the characterization of Geofence technology as a location system in Android. In the absence of no real information about the behavior of this system, a first step is taken to test different situations to be able to understand how Geofence acts in practice, and in addition, implement an algorithm that is able to track a person with a mobile phone

Single and Combined Effects of Cypermethrin and UVR Pre-Exposure in the Microalgae Phaeodactylum Tricornutum

Coastal marine microalgae are exposed to anthropogenic pollutants, including pesticides from aquaculture/agriculture/household uses. Some microalgae species, such as Phaeodactylum tricornutum, can induce and accumulate UV-absorbing compounds (UACs) upon ultraviolet radiation (UVR) exposure to prevent deleterious effects. Tolerance mechanisms activated by natural stressors might also protect organisms from anthropogenic stressors. This work assesses the effects of the insecticide cypermethrin (Cyp) and UVR in the marine microalgae P tricornutum. Considering the pro-oxidant properties of both stressors and UACs’ induction in P tricornutum, lethal and sublethal effects of Cyp were tested in cultures with and without UVR acclimation. After a 24-h exposure to 10 μg L−1 of technical Cyp or culture medium, UACs, growth, glutathione-S-transferase activity (GST), sulfhydryl groups (SH-g), and lipid peroxidation (LPO) were analyzed. Results showed differences in terms of growth between Cyp and Cyp + UVR pre-exposure. UACs’ content was induced after UVR acclimation and diminished after 24 h of growth in control and UVR pre-treated cultures, while levels remained constant under Cyp exposure. A single Cyp exposure exerted GST induction, SH-g depletion, and LPO increments. In UVR-acclimatized treatments, oxidative stress responses showed similar or more pronounced effects than the single chemical exposure, suggesting a potential additive effect of the UVR acclimation. The contrasting effects of Cyp + UVR observed between growth and biochemical responses suggest different compensatory mechanisms that need to be further investigated. Also, it highlights the need to include both lethal and sublethal endpoints to understand microalgae’s tolerance and its significance in the multiple stressors’ context.Fil: Cabrera, Joaquin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Marcoval, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Diaz Jaramillo, Mauricio Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Gonzalez, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentin

Effect of adding photoprotective compounds in the diet of Palaemon macrodactylus and its role under ultraviolet radiation

During the past two decades, it has shown an increase in ultraviolet radiation (UVR) and particularly the UVB (280-315nm) above Earth's surface. There is some evidence that UV-B radiation (280-315 nm) and the shorter wavelengths of UV-A (315-400 nm) can significantly affect the biota in waters up to 20 m deep and have adverse on aquatic organisms such as damage in the genetic material and high mortality. It has been shown that many species of seaweeds synthesize and accumulate high concentrations of photoprotective compounds and may constitute an interesting alternative as a supplement in aquaculture feeds. In the present work the effects of UVR were studied in the animal model Palaemon macrodactylus which is a successful invader, known as the Oriental Shrimp, native to estuaries and coastal waters of the Northwest Pacific. In the south western Atlantic, the species was found in Mar del Plata harbour, Argentina, probably introduced from the Pacific with discharged ballast water The aim of this study was to determine the bioaccumulation of photoprotective compounds (PPC) from diets added with red seaweeds meal of the family Halymeneaceae on juvenile P. macrodactylus and its possible protective role under conditions of stress by UVR. Juveniles of P. macrodactylus collected from Mar del Plata harbour (38° 03′ S; 57° 31′ W) were placed in PVC tanks under controlled conditions of temperature, pH, and salinity, under two feeding treatments: one group with basal diet (B) (45% protein, 8% lipid, 7% water and 7% ash) and the other 2% diet added with red seaweed meal of the family Halymeneaceae (diet B2). After 15 days animals were subjected to two radiations in four treatments, by triplicate: a) Control PAR (400-700nm) and diet B b) PAR+ UVR (280-700nm) and diet B c) PAR and diet B2, d) PAR+ UVR and diet B2. Survival, percentage of weight gain, concentrations of PPC and carotenoids were determined. In treatment b mortality of 16% was recorded after 72 hours of exposition to UVR, while in those fed with the 2% added diet, the same mortality was observed after 120 h of exposure. Survival in the treatments (a, c) was 100%. At the end of the exposure radiation period, percentage of weight gain was higher in animals fed with diet B (77%) nevertheless concentration of PPC and carotenoids (determined as OD g tissue-1) recorded the highest values in the animals corresponding to treatment c (PPC: 1.9 ± 0.38 DO g-1; carotenoids: 0.48 ± 0.1 OD g-1). although there were no significant differences between other treatments. These results suggest that, a diet added with red seaweeds rich in photoprotective compounds constitutes and interesting alternative, not only for its contribution to a better physiological state of animals under stress conditions by UVR, but also for the particularity of being invasive species with potential commercial use

Enfermedad de Paget extramamaria

Background and objective: Extramammary Paget disease (EMPD) has seldom been studied in Mediterranean populations. We aimed to review the characteristics of our patients with EMPD, the presence of a neoplasm in continuity, and the long-term course of the disease. Patients and methods: Retrospective observational study of 27 patients diagnosed with EMPD between 1990 and 2015. All clinical and pathology findings related to clinical course and outcomes were retrieved for analysis. Results: Twenty patients were women and 7 were men. Ages ranged from 42 to 88 years (median, 76 years). Lesions were in the following locations: vulva (16 cases), pubis-groin (5), perianal region (4), and axilla (2). Time from onset to diagnosis ranged from 1 to 60 months (median, 12 months) and maximum lesion diameter from 20 to 140mm (median, 55mm). In 3 cases (11.1%) EMPD was a secondary condition. None of the lesions developed on a previous cutaneous adnexal adenocarcinoma. Ten of the 24 primary EMPDs (41.7%) invaded the dermis. Eight of the 27 patients (29.6%) experienced local recurrence after the initial surgical treatment.Three patients (11.1%) died as a consequence of metastasis from the EMPD. Conclusions: The presence of an underlying cutaneous adnexal adenocarcinoma is uncommon, but it is not unusual to find an extracutaneous adenocarcinoma in continuity. Although EMPD is a slow-growing tumor, dermal invasion is frequent and metastasis is not uncommon. Local recurrence is common even after excision with wide margins and may be delated, so long term follow-up is essential

"Clown nose" as first manifestation of squamous cell carcinoma of the lung

Background: Skin metastases occur in 0.7% to 9% of all patients with cancer and are usually considered a late event in the evolution of most visceral carcinomas. The development of a nodular metastatic lesion on the nasal tip is known as clown nose sign. Main observation: We report a 64-year-old man that developed a nodular lesion on his nasal tip as first manifestation of squamous lung carcinoma. Conclusion: The biopsy of the cutaneous metastasis may be helpful to histopathologically confirm the suspected primary tumour avoiding invasive diagnostic procedures

Granuloma Annulare: a case-control study of possible associated diseases

Introduction: Granuloma annulare (GA) has been reported as associated with multiple diseases, mainly diabetes mellitus (DM), thyroid diseases, and dyslipidemia. However, the high prevalence of some of these illnesses makes it difficult to assess whether the association is real or fortuitous. Objectives: Our objective was to analyze the clinical features of GA patients and the possible associations. Methods: This is a retrospective observational study of 225 patients with biopsy-proven GA diagnosed between 2009 and 2019 in a referral university hospital in Barcelona, Spain. Clinical charts were reviewed to obtain clinical data. As a control group we used a random list of 225 patients diagnosed in the hospital traumatology department in the same period, matched by age and sex. Results: Diabetes was diagnosed in 40 GA patients (18%) (34 in the control group, 15%) and hypothyroidism in 33 (15%) (22 in the control group 9.8%); the differences were not significant. We also did not detect any association with uveitis, sarcoidosis, necrobiosis lipoidica, Sweet syndrome, HIV infection, hepatitis B, or hematological malignancies. We only detected a possible association with hepatitis C (6 GA patients, 2.7%, versus 0 controls, P = 0.03), and hypercholesterolemia (108 GA patients, 48%, versus 79 controls, 35%, P = 0.007). Conclusions: The possible pathogenic explanations for the association with hepatitis C and hypercholesterolemia seem unlikely. We consider that the association of GA with other diseases, including hypercholesterolemia and hepatitis C, is doubtful and that it there is no justification rule out possible associated diseases in patients with GA

Assessment of HSP70 and catalase in Brachidontes rodriguezii (d’Orbigny, 1842) a mussel from the Argentinean coast

Introduction: Intertidal ecosystems are complex environments of great importance for the ecological balance of coastal zones and are vulnerable areas to effects caused by natural stressors of global relevance such as temperature. Mussels, are often exposed to drastic variations of temperature and can show intracellular mechanisms of compensation that allow them to counteract exposure in the short and medium term at high temperatures. Heat shock proteins are chaperones that allow thermal stability of proteins against abrupt changes in temperature and catalase is an antioxidant enzyme thataid cells to fight against oxidative stress caused by diverse environmental stressors [1,2].Fil: Matos, Beatriz. Universidade Nova de Lisboa; PortugalFil: Pinoni, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Marcoval, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Diaz Jaramillo, Mauricio Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Souza Diniz, Mario. Universidade Nova de Lisboa; Portuga

Efectos de cantaxantina dietaria sobre el estres por radiacion ultravioleta en el camarón Artemesia longinaris

The aims of this research were to investigate the effects of diets with added synthetic canthaxanthin (10% parafarm) and to evaluate its possible protective role under ultraviolet radiation (UVR) in prawn Artemesia longinaris. Three isoproteic and isolipidic diets (41% protein and 12% lipid) containing 0 (C0), 100 (C100), and 300 (C300) mg of canthaxanthin kg-1 of diet were prepared. Before initiating the radiation experiment, prawns were fed with the different diets for a period of 21 d in order to determine a possible accumulation of carotenoids. Afterwards, animals were exposed to two radiation treatments for 7 d: a) photosynthetically active radiation (PAR, 400-700 nm), and b) total radiation (PAR+UVR, 280-700 nm), under controlled conditions (19 ± 2 °C, salinity = 33, pH = 7). In animals exposed to PAR+UVR treatment, survival varied between 50 and 83.33% with the highest value in animals fed diet C300. At the end of the experiment, significant statistical differences were registered in integument carotenoid concentration. Under UVR stress, the highest decrease in non-polar carotenoid and esterified astaxanthin were recorded in prawns fed diets containing canthaxanthin. Scavenging properties were evaluated by electron resonance spectroscopy (EPR) using the stable 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical. Prawns fed with C300 showed the greatest activity to quench DPPH. Results suggested that dietary canthaxanthin could be acting as an antioxidant against reactive oxygen species and produced high tolerance under UVR stress.Los objetivos de esta investigación fueron investigar los efectos de dietas adicionadas con cataxantina sintética (10% parafarm) y evaluar su posible papel protector bajo la radiación ultravioleta (RUV) en el camarón Artemesia longinaris. Se prepararon tres dietas isoprotéicas e isolipídicas (41% proteína y 12% lípidos) con 0 (C0), 100 (C100) y 300 (C300) mg de cantaxantina kg-1 de dieta. Previo al experimento de radiación, los camarones fueron alimentados con las diferentes dietas durante 21 d para determinar una posible acumulación de carotenoides. Posteriormente, los animales fueron expuestos a dos tratamientos de radiación durante 7 d: a) radiación fotosintéticamente activa (PAR, 400-700 nm), y b) radiación total (PAR+RUV, 280-700 nm), bajo condiciones controladas (19 ± 2 °C, salinidad = 33, pH = 7). En los individuos expuestos al tratamiento PAR+RUV, la supervivencia varió entre 50 y 83,33%, con el valor más alto en animales alimentados con dieta C300. Al final del experimento, se registraron diferencias estadísticas significativas en la concentración de carotenoides en el tegumento. Bajo estrés por RUV se registró la mayor disminución de carotenoides no polares y astaxantina esterificada en camarones alimentados con dietas con cataxantina. La capacidad antioxidante se evaluó mediante espectroscopía de resonancia electrónica (EPR) utilizando el radical estable 2,2-difenil-2-picrilhidrazilo (DPPH). Los camarones alimentados con C300 mostraron la mayor actividad evidenciada por el decaimiento de DPPH. Los resultados sugirieron que la cantaxantina dietaria podría estar actuando como un antioxidante contra las especies reactivas de oxígeno y producir una alta tolerancia bajo estrés por RUV