Lasalocid effects on dairy cows production of in a semi-intensive management system

Foram estudados os efeitos da lasalocida sobre a produção de leite, teor e percentagem de gordura no leite, variações de peso e condições corporais de 18 vacas leiteras do ecótipo Mantiqueira, com média de 75 dias pós-parto ao início do experimento, alimentadas com 1,0 kg de mistura de concentrados para cada 2,5 kg de leite produzidos acima dos primeiros 4,0 kg, capim elefante (Pennisetumpurpureum, var. Napier) picado, fornecido no cocho, e pastagem de Bracharia brizantha. Utilizou-se um delineamento em blocos casualizados e os tratamentos consistiram na administração diária de 0,200 e 300 mg de lasalocida/animal. O experimento teve duração de 17 semanas, sendo as quatro primeiras destinadas ao período de adaptação. Independente da dose empregada a lasalocida não alterou significativamente a produção de leite e a produção de leite corrigida a 4% de gordura, porém o grupo tratado com 200 mg apresentou maior porcentagem de gordura no leite (P < 0,05). Os grupos tratados com 200 e 300 mg apresentaram ganhos de peso, porém não estatisticamente menores em relação aos animais controle, quando os dados foram analisados como percentagem de peso vivo. Não foi observada alteração na condição corporal dos animais.The effects of lasalocid over milk, milk fat content, live weight and body condition, were analysed on 18 “Mantiqueira” cows, 75 days after parturition in the begining of the trial. Cows were fed 1 kg concentrate mixture for each 2,5 kg of milk produced over the first 4.0 kg of milk, chopped elephant grass (Pennisetum purpureum, Napier var), and Brachiaria brizantha pasture. A randomized block design was used with 0, 200 or 300 mg of lasalocid/animal/day as treatments. The experimental period lasted 17 weeks, and first four weeks were used as an adaptation period to the ionophore. Lasalocid had no effects on milk or fat corrected milk production, but the 200 mg lasalocid group presented higher milk fat percentages (P < 0.05). The 200 and 300 mg lasalocid treatments groups showed lower (but not statistically) live weight gain related to control, when the data wereanalysed as percentage of live weight. There was no variation on cows body condition

Transcriptomic analysis suggests candidate genes for hygienic behavior in African-derived Apis mellifera honeybees

Hygienic behavior is a social defense mechanism against parasites and pathogens in honeybees. We studied the genetic basis of hygienic behavior in African-derived Apis mellifera by performing RNA sequencing on brains of individual honeybee workers observed performing hygienic behavior, in order to identify expression changes linked with this behavior. We also used the transcriptome data to search for single nucleotide polymorphism (SNP) variation in genes previously associated with the trait in other A. mellifera populations. The analysis revealed 49 differentially expressed genes (DEG), most of them upregulated during hygienic acts. One DEG, Apidaecin, also showed SNP variation across samples, providing a promising candidate gene for both expression-based and heritable variation in hygienic behavior. We also identified 27 additional SNPs in the coding regions of five candidate genes previously linked with honeybee hygienic behavior. These results provide a useful starting place to analyze the genetic basis of hygienic behavior in African-derived honeybees

Somatotrophic axis genes on growth traits in a bovine F2 population

O objetivo deste trabalho foi avaliar a associação dos polimorfismos dos genes bGH, IGF-1 e PIT-1 com características de peso e ganho de peso numa população F2 de bovinos (Gir x Holandês), pela técnica de PCR-RFLP. As freqüências alélicas A e B, do gene PIT-1, e dos genótipos AA, AB e BB, nas populações parentais foram semelhantes entre si, mas diferentes das freqüências nas populações cruzadas F1 e F2, para esse gene. Quanto ao gene bGH, os animais da raça Holandesa apresentaram freqüência de 100% para o alelo E e os animais da raça Gir, 92% para o alelo F, resultando em alta freqüência de indivíduos heterozigotos nas populações F1 e F2. Quanto ao gene IGF-1, todos os animais da raça Holandesa eram heterozigotos (AB) e, nos animais Gir, a maioria dos indivíduos foi de homozigotos (AA), o que resultou em alta freqüência do alelo A nas populações F1 e F2. Foram encontradas associações significativas do alelo A do gene PIT-1 com as características de peso aos 60, 205, 365 dias e ganho de peso do nascimento aos 60 dias. Em bGH, observou-se efeito significativo do alelo E para peso aos 365 dias e ganho de peso do nascimento aos 60 dias, enquanto o efeito do alelo A do IGF-1 foi significativo somente para peso ao nascimento. Os alelos identificados podem ser usados como marcadores no melhoramento animal.The objective of this work was to evaluate the association between polymorphisms of bGH, IGF-1 and PIT-1 genes with weight and weight gain traits in a bovine F2 population derived from Holstein x Gyr crosses, using the PCR-RFLP technique. Allelic frequencies A and B of PIT-1 gene and AA, AB, and BB genotypes were similar between parental populations but different from F1 and F2 crossed populations. For the bGH gene, Holstein animals showed allelic frequency of 100% for E allele, while Gyr animals showed allelic frequency of 92% for F allele, resulting in high frequency of heterozygous animals in F1 and F2 populations. For the IGF-1 gene, all Holstein were heterozygous (AB) and most Gyr were homozygous (AA), resulting in a high frequency of A allele in F1 and F2 populations. Significant associations were found between PIT-1 allele A with traits weight at 60, 205 and 365 days and weight gain from birth to 60 days. For IGF-1, significant association was found between A allele and birth weight. For bGH gene, significant associations were found between E allele and 365 days weight and weight gain from birth to 60 days. The selected alleles could be used as markers in animal breeding for these traits

Análise genética de características produtivas e reprodutivas em populações multirraciais de bovinos leiteiros

The objective of this work was to determine whether the random regression model using linear splines (RRMLS) is suitable to estimate the genetic parameters for productive and reproductive traits of a multiple-breed dairy cattle population, as well as to investigate the effect of the genetic group of the progeny on the genetic merit of the sire. The multiple-trait model (MTM) and the RRMLS with one knot fitted for every genetic group were used to obtain the genetic parameters. Records of 1/2 Holstein + 1/2 Gyr (1/2HG), 5/8 Holstein + 3/8 Gyr (5/8HG), and 3/4 Holstein + 1/4 Gyr (3/4HG) crossbreed dams were considered. The RRMLS showed better fitting. The additive and residual variances estimated by the MTM and the RRMLS were similar. Heritability varied from 0.20 to 0.33 for age at first calving, from 0.09 to 0.22 for first lactation length, and from 0.15 to 0.35 for first lactation 305-day milk yield, according to the genetic composition of the dams. The RRMLS is suitable to estimate the genetic parameters for productive and reproductive traits of multiple-breed dairy cattle populations. The genetic merit of the sires is affected by the genetic group of the progeny by which they are evaluated.O objetivo deste trabalho foi verificar se o modelo de regressão aleatória com polinômios lineares do tipo spline (MRAPLS) é adequado para estimar os parâmetros genéticos de características produtivas e reprodutivas em uma população multirracial de bovinos leiteiros, bem como investigar o efeito do grupo genético da progênie sobre o mérito genético do touro. Foram utilizados o modelo multicaracterístico (Mult) e o MRAPLS com nós ajustados aos grupos genéticos, para obtenção dos parâmetros genéticos. Foram considerados os registros de vacas 1/2 Holandesa + 1/2 Gir (1/2HG), 5/8 Holandesa + 3/8 Gir (5/8HG) e 3/4 Holandesa + 1/4 Gir (3/4HG). O MRAPLS apresentou os melhores ajustes. As variâncias aditivas e residuais estimadas por Mult e MRAPLS foram semelhantes. As herdabilidades variaram de 0,20 a 0,33 para idade ao primeiro parto, de 0,09 a 0,22 para duração da primeira lactação e de 0,15 a 0,35 para produção de leite em até 305 dias na primeira lactação, de acordo com a composição genética das vacas. O MRAPLS é adequado para estimar parâmetros genéticos de características produtivas e reprodutivas em populações multirraciais de bovinos leiteiros. O mérito genético do touro é influenciado pelo grupo genético da progênie por meio do qual ele é avaliado

Identification of selection signatures in livestock species

The identification of regions that have undergone selection is one of the principal goals of theoretical and applied evolutionary genetics. Such studies can also provide information about the evolutionary processes involved in shaping genomes, as well as physical and functional information about genes/genomic regions. Domestication followed by breed formation and selection schemes has allowed the formation of very diverse livestock breeds adapted to a wide variety of environments and with special characteristics. The advances in genomics in the last five years have enabled the development of several methods to detect selection signatures and have resulted in the publication of a considerable number of studies involving livestock species. The aims of this review are to describe the principal effects of natural/artificial selection on livestock genomes, to present the main methods used to detect selection signatures and to discuss some recent results in this area. This review should be useful also to research scientists working with wild animals/non-domesticated species and plant biologists working with breeding and evolutionary biology

Genetic parameters of milk production and reproduction traits of Girolando cattle in Brazil

The objective of this study was to estimate the heritability and genetic, phenotypic, and environmental correlations between 305-day milk yield (305MY), age at first calving (AFC), calving interval (CI) and dry period length (DPL), as well as to compare the productive and reproductive performances of several groups of Girolando cows during their first, second and third parturitions in Brazil. Genetic parameters were estimated using uni- and bi-trait analyses via restricted maximum likelihood (REML). Heritability estimates were similar between uni- and bi-trait analyses and varied from 0.20 to 0.28, 0.00 to 0.08 and 0.07 to 0.14 for 305MY, CI and DPL, respectively, whereas AFC was 0.20. Genetic correlations between 305MY and AFC (–0.49) or DPL (–0.40 to –0.79) show a favourable association. However, an unfavourable genetic association was verified between 305MY and CI (0.59) in the first calving. Environmental and phenotypic correlations between 305MY and CI or DPL showed a favourable association (–0.25 to –0.42 and –0.14 to –0.44). Concerning the cow breed group, a decrease in 305MY was observed as the fraction of the Gyr breed increased, whereas animals from the 1/2 Holstein +1/2 Gyr crossbreeding group yielded better performances for AFC and CI. These results reveal large genetic, phenotypic and environmental variations that could be used in selection programmes

Ãndices e causas de condenação de méis brasileiros

Através de determinações físico-químicas, foram analisadas 721 amostras de méis oriundas de diferentes estados brasileiros, com o objetivo de avaliar o estado de conservação das mesmas e também detectar possíveis fraudes ocorridas. As amostras (68,3 % oriundas do Estado de São Paulo, 9,9% de Minas Gerais, 8,1% de Santa Catarina; 3,8% do Paraná; 2,8% do Rio de Janeiro e os restantes 7,0%, dos demais Estados) foram enviadas ao laboratório do Centro de Apicultura Tropical, em Pindamonhangaba, SP, por apicultores, comerciantes e consumidores. Constatou-se que 67,96% dos méis recebidos apresentaram todos os índices de qualidade dentro das faixas consideradas como aceitáveis pela legislação brasileira. As maiores causas de condenação foram: 18,3% das amostras por apresentar reação de Fiehe positiva; 5,5%, devido ao teor de HMF (hidroximetilfurfural) acima do limite legal (40 mg/Kg) e 6,2% das amostras, devido ao conteúdo de umidade acima do permitido (20%). Os resultados indicam que o principal problema de nossos méis está na adequação das tecnologias de processamento empregadas, bem como na conscientização por parte de quem manipula o produto, das condições adequadas de acondicionamento e conservação, além da primordial necessidade de colhê-lo com teor de umidade dentro do determinado, visto que tais requisitos, quando não observados, podem levar a uma rápida depreciação da qualidade do produto

Genetic parameters for milk yield and lactation persistency using random regression models in Girolando cattle

A total of 32,817 test-day milk yield (TDMY) records of the first lactation of 4,056 Girolando cows daughters of 276 sires, collected from 118 herds between 2000 and 2011 were utilized to estimate the genetic parameters for TDMY via random regression models (RRM) using Legendre’s polynomial functions whose orders varied from 3 to 5. In addition, nine measures of persistency in milk yield (PSi) and the genetic trend of 305-day milk yield (305MY) were evaluated. The fit quality criteria used indicated RRM employing the Legendre’s polynomial of orders 3 and 5 for fitting the genetic additive and permanent environment effects, respectively, as the best model. The heritability and genetic correlation for TDMY throughout the lactation, obtained with the best model, varied from 0.18 to 0.23 and from −0.03 to 1.00, respectively. The heritability and genetic correlation for persistency and 305MY varied from 0.10 to 0.33 and from −0.98 to 1.00, respectively. The use of PS7 would be the most suitable option for the evaluation of Girolando cattle. The estimated breeding values for 305MY of sires and cows showed significant and positive genetic trends. Thus, the use of selection indices would be indicated in the genetic evaluation of Girolando cattle for both traits