Expression patterns of GmAP2/EREB-Like transcription factors involved in soybean responses to water deficit.

Soybean farming has faced several losses in productivity due to drought events in the last few decades. However, plants have molecular mechanisms to prevent and protect against water deficit injuries, and transcription factors play an important role in triggering different defense mechanisms. Understanding the expression patterns of transcription factors in response to water deficit and to environmental diurnal changes is very important for unveiling water deficit stress tolerance mechanisms. Here, we analyzed the expression patterns of ten APETALA2/Ethylene Responsive Element Binding-like (AP2/ EREB-like) transcription factors in two soybean genotypes (BR16: drought-sensitive; and Embrapa 48: drought-tolerant). According to phylogenetic and domain analyses, these genes can be included in the DREB and ERF subfamilies. We also analyzed a GmDRIP-like gene that encodes a DREB negative regulator. We detected the up-regulation of 9 GmAP2/EREB-like genes and identified transcriptional differences that were dependent on the levels of the stress applied and the tissue type analyzed (the expression of the GmDREB1F-like gene, for example, was four times higher in roots than in leaves). The GmDRIP-like gene was not induced by water deficit in BR16 during the longest periods of stress, but was significantly induced in Embrapa 48; this suggests a possible genetic/molecular difference between the responses of these cultivars to water deficit stress. Additionally, RNAseq gene expression analysis over a 24-h time course indicates that the expression patterns of several GmDREB-like genes are subject to oscillation over the course of the day, indicating a possible circadian regulation

Sistema de Informação da Melhor Idade - SIMI: uma proposta de aplicativo para a promoção da saúde na terceira idade.

A preocupação com a promoção da saúde do idoso vem crescendo nos últimos anos devido às mudanças na maneira de valorizá-la como determinante para a melhoria da qualidade de vida e do exercício da cidadania. A emergência de tecnologias móveis, como tablets e smartphones tem tido seu uso expandido para toda a população, inclusive a de pessoas com 60 anos ou mais. Baseado nesse cenário propõe-se o desenvolvimento do Sistema de Informação da Melhor Idade (SIMI), voltado para a promoção da saúde do idoso, para que se torne possível obter informações sobre o histórico de saúde do idoso, informações nutricionais relacionadas à prevenção de doenças, e conteúdos de eventos de promoção da qualidade de vida. Este relato de experiência também apresenta algumas ferramentas metodológicas utilizadas para o levantamento de requisitos e definições do projeto do SIMI, auxiliando no desenvolvimento de um recurso tecnológico inovador na promoção da inclusão e integração sociais, autonomia e segurança do idoso

Transcriptome-wide identification of reference genes for expression analysis of soybean responses to drought stress along the day.

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Discovery of a strong magnetic field in the rapidly rotating B2Vn star HR 7355

We report the detection of a strong, organized magnetic field in the helium-variable early B-type star HR 7355 using spectropolarimetric data obtained with ESPaDOnS on the 3.6-m Canada-France-Hawaii Telescope within the context of the Magnetism in Massive Stars (MiMeS) Large Program. HR 7355 is both the most rapidly rotating known main-sequence magnetic star and the most rapidly rotating helium-strong star, with $v \sin i$ = 300 $\pm$ 15 km s$^{-1}$ and a rotational period of 0.5214404 $\pm$ 0.0000006 days. We have modeled our eight longitudinal magnetic field measurements assuming an oblique dipole magnetic field. Constraining the inclination of the rotation axis to be between $38^{\circ}$ and $86^{\circ}$, we find the magnetic obliquity angle to be between $30^{\circ}$ and $85^{\circ}$, and the polar strength of the magnetic field at the stellar surface to be between 13-17 kG. The photometric light curve constructed from HIPPARCOS archival data and new CTIO measurements shows two minima separated by 0.5 in rotational phase and occurring 0.25 cycles before/after the magnetic extrema. This photometric behavior coupled with previously-reported variable emission of the H$\alpha$ line (which we confirm) strongly supports the proposal that HR 7355 harbors a structured magnetosphere similar to that in the prototypical helium-strong star, $\sigma$ Ori E.Comment: 6 pages, 3 figures. Accepted for publication in MNRAS Letter

HR 5907: Discovery of the most rapidly rotating magnetic B-type star by the MiMeS Collaboration

We report the discovery and analysis of a very strong magnetic field in the rapidly rotating early B-type star HR 5907, based on observations obtained as part of the Magnetism in Massive Stars (MiMeS) project. We infer a rotation period of 0.508276 +0.000015/-0.000012 d from photometric and H{\alpha} EW measurements, making this the shortest period, non-degenerate, magnetic massive star known to date. From the comparison of IUE UV and optical spectroscopy with LTE BRUCE/KYLIE models we find a solid-angle integrated, uniform black-body temperature of 17 000 \pm 1000 K, a projected rotational velocity of 290 \pm 10 km/s, an equatorial radius of 3.1 \pm 0.2 R_sun, a stellar mass of 5.5 \pm 0.5 M_sun, and an inclination angle of the rotation axis to our line-of-sight of 70 \pm 10\circ. Our measurements of the longitudinal magnetic field, which vary between -500 and -2000 G, phase coherently with the rotation period and imply a surface dipole field strength of \sim15.7 kG. On the other hand, from fits to mean Least-Squares Deconvolved Stokes V line profiles we infer a dipole field strength of \sim10.4 kG. This disagreement may result from a magnetic configuration more complex than our model, and/or from the non-uniform helium surface abundance distribution. In either case we obtain a magnetic obliquity nearly aligned with the rotation axis ({\beta} = 7+2/-1\circ). Our optical spectroscopy also shows weak variability in carbon, silicon and nitrogen lines. The emission variability in hydrogen Balmer and Paschen lines indicates the presence of a dense, highly structured magnetosphere, interpreted as a centrifugally supported, magnetically confined circumstellar disk.Comment: 19 pages, 17 figures (highly compressed), 3 tables, accepted for publication by MNRA