Single atom detection on an atom chip with integrated optics

The subject of this thesis is the miniaturization and integration of detectors for the detection of single rubidium atoms on an atom chip. For this purpose three different detectors were developed for atom detection by absorption or fluorescence: i) A fluorescence detector was realized by mounting a tapered lensed fiber perpendicular to a collection fiber. ii) An absorption detector was built by placing a multi-mode fiber in-line with a tapered lensed fiber. iii) A tunable cavity detector was built from two single mode fibers mounted on the chip surface. To mount the detectors on the planar surface of the atom chip retaining structures by means of lithographical techniques were developed. Those structures allow a very accurate and stable passive fiber alignment. To test the individual detectors an atomic ensemble of thermal rubidium atoms was prepared in a magneto-optical trap. Then the atoms were transferred into magnetic micro-traps of the atom chip and were guided to one of the detectors. Each integrated detector has been evaluated by several characterization measurements. With the fluorescence detector the highest single atom detection efficiency of 54\% was attained. Thereby it was possible to perform quantitative measurements for the magnetically guided atoms on a single atom level and to study fundamental properties of the confined atoms

A simple integrated single-atom detector

We present a reliable and robust integrated fluorescence detector capable of detecting single atoms. The detector consists of a tapered lensed single-mode fiber for precise delivery of excitation light and a multimode fiber to collect the fluorescence. Both are mounted in lithographically defined SU-8 holding structures on an atom chip. Rb87 atoms propagating freely in a magnetic guide are detected with an efficiency of up to 66%, and a signal-to-noise ratio in excess of 100 is obtained for short integration times.Comment: 3 pages, 3 figure

Improved Protoporphyrin IX-Guided Neurosurgical Tumor Detection with Frequency-Domain Fluorescence Lifetime Imaging

Precise intraoperative brain tumor visualization supports surgeons in achieving maximal safe resection. In this sense, improved prognosis in patients with high-grade gliomas undergoing protoporphyrin IX fluorescence-guided surgery has been demonstrated. Phase fluorescence lifetime imaging in the frequency-domain has shown promise to distinguish weak protoporphyrin IX fluorescence from competing endogenous tissue fluorophores, thus allowing for brain tumor detection with high sensitivity. In this work, we show that this technique can be further improved by minimizing the crosstalk of autofluorescence signal contributions when only detecting the fluorescence emission above 615 nm. Combining fluorescence lifetime and spectroscopic measurements on a set of 130 ex vivo brain tumor specimens (14 low- and 56 high-grade gliomas, 39 meningiomas and 21 metastases) coherently substantiated the resulting increase of the fluorescence lifetime with respect to the detection band employed in previous work. This is of major interest for obtaining a clear-cut distinction from the autofluorescence background of the physiological brain. In particular, the median fluorescence lifetime of low- and high-grade glioma specimens lacking visual fluorescence during surgical resection was increased from 4.7 ns to 5.4 ns and 2.9 ns to 3.3 ns, respectively. While more data are needed to create statistical evidence, the coherence of what was observed throughout all tumor groups emphasized that this optimization should be taken into account for future studies

Aminocyclopentitols from <i>N</i>-Alkylpyridinium Salts: A photochemical approach

The photolysis of N‐alkylpyridinium halides 9a–e in alkaline H2O gave 6‐azabicyclo[3.1.0]hexenol derivatives 10a–e. N–Substituents bearing ether, acetal, and alcohol functions were found to do not adversely influence the photochemical reaction course. The free OH groups of the N–(3‐hydroxypropyl) derivative 10d were protected by benzoylation. The ensuing dibenzoate 14 underwent stereocontrolled opening of the aziridine ring on reaction with MeSH/BF3 to give a thioether 15. With benzoic acid in CHCI3, 10d gave the 4‐hydroxy‐5‐aminocyclopent‐2‐enyl benzoate 11. The meso‐2‐aminocyclopent‐4‐ene‐1,3‐diol 12 was obtained by hydrolysis of 11. On reaction with Boc2O and NaI, the aziridine ring of 14 was converted to a bicyclic compound 17. Hydrolysis of 17 provided the trans‐1,3‐diol 18, the epimer of 12. Face‐selective dihydroxylation of Boc‐protected 12 gave a meso‐aminocyclopentanetetrol 23 which was characterized upon peracetylation. Dihydroxylation of 15 provided a racemic analogue of epi‐mannostatin A (26)