Prunus spinosa fresh fruit juice: antioxidant activity in cell-free and cellular systems.

The antioxidant activity was assessed of fresh juice from Prunus spinosa L. fruit (Rosaceae) growing wild in Urbino (central Italy) by using different cell-free in vitro analytical methods: 5-lipoxygenase test, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, and oxygen radical absorbance capacity (ORAC). Trolox was used as the reference antioxidant compound. In the 5-lipoxygenase and DPPH tests the fresh fruit juice of P. spinosa showed good antioxidant activity when compared with Trolox, while the ORAC value was 36.0 μmol eq. Trolox /g of fruit. These values are in accord with data reported in the literature for small fruits such as Vaccinium, Rubus and Ribes. The antioxidant capacity in cell-free systems of P. spinosa juice has been compared with its cytoprotective – bona fide antioxidant activity in cultured human promonocytes (U937 cells) exposed to hydrogen peroxide. The antioxidant activity of red berries has been correlated with their anthocyanin content. The results of this study indicate that the three most representative anthocyanins in P.spinosa fruit juice (cyanidin-3-rutinoside, peonidin-3-rutinoside and cyanidin-3-glucoside) are likely to play an important role in its antioxidant properties

Acute heart failure after Caesaerean section: peri-partum or tako-tsubo cardiomiopathy?

A young woman undergoes Caesarean section at the 39th week of pregnancy: shortly after she develops acute cardiorespiratory failure. The electrocardiography shows sinus tachycardia and right bundle branch block. The ventriculography confirms the decrease of the pump function and the mid-ventricular ballooning of the left ventricle; the differential diagnosis is between peri-partum cardiomyopathy and stress induced tako-tsubo cardiomyopathy: the sudden onset, the results of the ventriculography and the complete recovery after 11 days of treatment for acute heart failure led the diagnosis towards tako-tsubo cardiomyopathy

The Intra-Group Baryons in the LEO I Pair From the VST Early-Type GAlaxy Survey

In this paper we present the deep, wide-field and multi-band imaging of the LEO I pair NGC 3379-NGC 3384, from the VST Early-type GAlaxy Survey (VEGAS). The main goal of this study is to map the intra-group baryons in the pair, in the form of diffuse light and globular clusters (GCs). Taking advantage from the large covered area, which extends for ∼ 3.9 square degrees around the pair, and the long integration time, we can map the light distribution out to ∼ 63 kpc and down to ∼ 30 mag/arcsec2 in the g band and ∼ 29 mag/arcsec2 in the r band, deeper than previous data available for this target. The map of the intra-group light (IGL) presents two very faint (μg ∼ 28-29 mag/arcsec2) streams protruding from the brightest group member NGC 3379 and elongated toward North-West and South. We estimate that the fraction of the stellar halo around NGC 3379 plus the intra-group light is (Formula presented.) in both g and r bands, with an average color g-r = 0.75 ± 0.04 mag. The color distribution of the GCs appears multi-modal, with two dominant peaks at (u-r) = 1.8 mag and (u-r) = 2.1 mag, respectively. The GC population stretches from North-East to South-West and from North-West to South of the pair, in the last case overlapping with the streams of IGL, as well as the PNe distribution found by Hartke et al. (Hartke et al., A&amp;A, 2020, 642, A46) and Hartke et al. (Hartke et al., 2022). Since these structures are elongated in the direction of the two nearby galaxies M96 and NGC 3338, they could be the remnant of a past gravitational interactions with the pair.</p

Integrin-Targeting Dye-Doped PEG-Shell/Silica-Core Nanoparticles Mimicking the Proapoptotic Smac/DIABLO Protein

Cancer cells demonstrate elevated expression levels of the inhibitor of apoptosis proteins (IAPs), contributing to tumor cell survival, disease progression, chemo-resistance, and poor prognosis. Smac/DIABLO is a mitochondrial protein that promotes apoptosis by neutralizing members of the IAP family. Herein, we describe the preparation and in vitro validation of a synthetic mimic of Smac/DIABLO, based on fluorescent polyethylene glycol (PEG)-coated silica-core nanoparticles (NPs) carrying a Smac/DIABLO-derived pro-apoptotic peptide and a tumor-homing integrin peptide ligand. At low \u3bcM concentration, the NPs showed significant toxicity towards A549, U373, and HeLa cancer cells and modest toxicity towards other integrin-expressing cells, correlated with integrin-mediated cell uptake and consequent highly increased levels of apoptotic activity, without perturbing cells not expressing the \u3b15 integrin subunit

Peripartum cardiomyopathy

Peripartum cardiomyopathy is an uncommon form of congestive heart failure associated with systolic dysfunction of left ventricle. The onset is characterised by symptoms of heart failure occurring between the last month of pregnancy and 5-6 months postpartum. The early diagnosis and the institution of medical treatment for this disease are essential because the inadequate management may affect the patient’s long-term prognosis and can lead to severe complications, including death.Currently its aetiology is not completely understood. Many aetiopathogenetic hypotheses have been formulated: inflammation, viral agents, autoimmune processes. In the last years, evidences aroused for a role of prolactin and its 16 kDa metabolite in reducing cardiomyocite metabolic activity and contraction. In this article we have reviewed the current literature with special emphasis on the role of prolactin and the related current treatment strategies. In particular, bromocriptine appears promising, even if women need to be informed that the drug stops the production of breastmilk. Further researchers, such as large multicenter trials, are needed to decide the best treatment for the women suffering of this disease

In vitro characterization of iridoid and phenylethanoid glycosides from Cistanche phelypaea for nutraceutical and pharmacological applications

"Desert hyacinths" are a remarkable group of parasitic plants belonging to genus Cistanche, including more than 20 accepted species typically occurring in deserts or coastal dunes parasitizing roots of shrubs. Several Cistanche species have long been a source of traditional herbal medicine or food, being C.&nbsp;deserticola and C.&nbsp;tubulosa the most used in China. This manuscript reports the isolation and identification of some phenylethanoid and iridoid glycosides, obtained from the hydroalcoholic extract of C.&nbsp;phelypaea collected in Spain. The present study aims to characterize the antioxidant activity of C.&nbsp;phelypaea metabolites in the light of their application in nutraceutical and cosmeceutical industries and the effect of acetoside, the most abundant metabolite in C.&nbsp;phelypaea extract, on human keratinocyte and pluripotent stem cell proliferation and differentiation. Our study demonstrated that acetoside, besides its strong antioxidant potential, can preserve the proliferative potential of human basal keratinocytes and the stemness of mesenchymal progenitors necessary for tissue morphogenesis and renewal. Therefore, acetoside can be of practical relevance for the clinical application of human stem cell cultures in tissue engineering and regenerative medicine

Does the virial mass drive the intra-cluster light? The relationship between the ICL and Mvir_{vir} from VEGAS

In this Letter we revisit the relationship between the fraction of the intra-cluster light (ICL) and both the virial mass and the fraction of Early Type Galaxies in the host halo. This is based on a statistically significant and homogeneous sample of 22 groups and clusters of galaxies in the local Universe ($z \leq 0.05$), obtained with the VST Early-type GAlaxy Survey (VEGAS). Taking advantage of the long integration time and large area of the VEGAS images, we are able to map the galaxy outskirts and ICL down to $\mu_g$ $\geq$ 29-30 mag/arcsec$^2$ and out to hundreds of kpc. With this data-set, we have expanded the sample of ICL measurements, doubling the previous measures available from the literature for z $\leq$ 0.05. The main result of this work is the lack of any significant trend between the fraction of ICL and the virial mass of the host environment, covering a wide range of virial masses ( $\sim$ $10^{12.5} \leq M_{vir} \leq 10^{15.5} M_{\odot}$), in agreement with some theoretical studies. Since the new data points are all derived with the same methodology and from the same observational setup, and all have comparable depth, the large observed scatter indicates an intrinsic variation in the ICL fraction.On the other hand, there is a weak relation between the fraction of ICL and the fraction of Early Type Galaxies in the host halo, where a larger fraction of ICL is found in groups and clusters of galaxies dominated by earlier morphological types, indicating a connection between the ICL and the dynamical state of the host system.Comment: Letter to the editor, accepted for publication in A&A. 8 pages, 3 figure

Development of Artificial Plasma Membranes Derived Nanovesicles Suitable for Drugs Encapsulation

Extracellular vesicles (EVs) are considered as promising nanoparticles theranostic tools in many physiological and pathological contexts. The increasing clinical employment of therapeutic nanoparticles is contributing to the development of a new research area related to the design of artificial EVs. To this aim, different approaches have been described to develop mimetic biologically functional nanovescicles. In this paper, we suggest a simplified procedure to generate plasma membranes-derived nanovesicles with the possibility to efficiently encapsulate different drugs during their spontaneously assembly. After physical and molecular characterization by Tunable Resistive Pulse Sensing (TRPS) technology, transmission electron microscopy and flow cytometry, as a proof of principle, we have loaded into mimetic EVs the isoquinoline alkaloid Berberine chloride, the chemotherapy compounds Temozolomide or Givinostat. We demonstrated the fully functionality of these nanoparticles in drugs encapsulation and cell delivery, showing, in particular, similar cytotoxic effect of direct cell culture administration of the anticancer drugs. In conclusion, we have documented the possibility to easily generate scalable nanovesicles with specific therapeutic cargo modifications useful in different drugs delivery contexts

Correction to: Accuracy and inter-observer agreement of the nice and kudo classifications of superficial colonic lesions: a comparative study

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