1,446 research outputs found
Regulation of ykrL (htpX) by Rok and YkrK, a Novel Type of Regulator in Bacillus subtilis
Expression of ykrL of Bacillus subtilis, encoding a close homologue of the Escherichia coli membrane protein quality control protease HtpX, was shown to be upregulated under membrane protein overproduction stress. Using DNA affinity chromatography, two proteins were found to bind to the promoter region of ykrL: Rok, known as a repressor of competence and genes for extracytoplasmic functions, and YkrK, a novel type of regulator encoded by the gene adjacent to ykrL but divergently transcribed. Electrophoretic mobility shift assays showed Rok and YkrK binding to the ykrL promoter region as well as YkrK binding to the ykrK promoter region. Comparative bioinformatic analysis of the ykrL promoter regions in related Bacillus species revealed a consensus motif, which was demonstrated to be the binding site of YkrK. Deletion of rok and ykrK in a PykrL-gfp reporter strain showed that both proteins are repressors of ykrL expression. In addition, conditions which activated PykrL (membrane protein overproduction, dissipation of the membrane potential, and salt and phenol stress) point to the involvement of YkrL in membrane protein quality control
From St\"{a}ckel systems to integrable hierarchies of PDE's: Benenti class of separation relations
We propose a general scheme of constructing of soliton hierarchies from
finite dimensional St\"{a}ckel systems and related separation relations. In
particular, we concentrate on the simplest class of separation relations,
called Benenti class, i.e. certain St\"{a}ckel systems with quadratic in
momenta integrals of motion.Comment: 24 page
Exploring the impact of participative place-based community archaeology in rural Europe: Community archaeology in rural environments meeting societal challenges
This paper reviews the aims, context, approach and early outcomes of a
new transnational participative archaeology project focussed on rural
village communities. âCommunity Archaeology in Rural Environments
Meeting Societal Challengesâ (CARE-MSoC) includes three European
countries where participative community archaeology is new- the Czech
Republic, the Netherlands and Poland. CARE-MSoC aims to explore the
feasibility, value and impact of excavation by rural residents within their
home communities by using a method which can be deployed
anywhere and which in the UK has been shown to advance knowledge
of the past while also delivering a wide range of social and heritage
benefits: multiple test pit excavation within inhabited villages. Data
presented here from the Czech Republic, the Netherlands and Poland
show the activity to be popular and effective here in benefitting people
while also attracting, sustaining and growing local interest in heritage
participation in all three countries
Exploring the impact of participative place-based community archaeology in rural Europe: Community archaeology in rural environments meeting societal challenges
Effective pressure interface law for transport phenomena between an unconfined fluid and a porous medium using homogenization
We present modeling of the incompressible viscous flows in the domain
containing an unconfined fluid and a porous medium. For such setting a rigorous
derivation of the Beavers-Joseph-Saffman interface condition was undertaken by
J\"ager and Mikeli\'c [SIAM J. Appl. Math. \rm 60 (2000), p. 1111-1127] using
the homogenization method. So far the interface law for the pressure was
conceived and confirmed only numerically. In this article we justify rigorously
the pressure jump condition using the corresponding boundary layer
Role of PostâAcute Care in Readmissions for Preexisting HealthcareâAssociated Infections
Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/153607/1/jgs16208.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/153607/2/jgs16208_am.pd
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Seshat: The Global History Databank
The vast amount of knowledge about past human societies has not been systematically organized and, therefore, remains inaccessible for empirically testing theories about cultural evolution and historical dynamics. For example, what evolutionary mechanisms were involved in the transition from the small-scale, uncentralized societies, in which humans lived 10,000 years ago, to the large-scale societies with an extensive division of labor, great differentials in wealth and power, and elaborate governance structures of today? Why do modern states sometimes fail to meet the basic needs of their populations? Why do economies decline, or fail to grow? In this article, we describe the structure and uses of a massive databank of historical and archaeological information, Seshat: The Global History Databank. The data that we are currently entering in Seshat will allow us and others to test theories explaining how modern societies evolved from ancestral ones, and why modern societies vary so much in their capacity to satisfy their membersâ basic human needsPeer reviewedFinal Published versio
MesobanK UK: an international mesothelioma bioresource.
Malignant pleural mesothelioma causes the greatest societal burden of all the asbestos-related diseases. Progress in better understanding tumour biology will be facilitated by the availability of quality-assured annotated tissue. MesobanK has been created to establish a bioresource of pleural mesothelioma tissue linked to detailed anonymised clinical data. When complete, the bioresource will comprise a 750-patient tissue microarray and prospectively collected tissue, blood and pleural fluid from 300 patients with mesothelioma. Twenty-six new cell lines have also been developed. MesobanK meets all appropriate ethical and regulatory procedures and has recently opened to requests for tissue and data.RCR and DMR are part funded by the Cambridge Biomedical Research Centre and the Cambridge Cancer Centre. RCR is also funded by the NIHR Clinical Research Network: Eastern.This is the final version of the article. It first appeared from BMJ via http://dx.doi.org/10.1136/thoraxjnl-2015-20749
Use of a Novel Infrared Wavelength-tunable Laser Mueller-matrix Polarimetric Scatterometer to Measure Nanostructured Optical Materials
Nanostructured optical materials, for example, metamaterials, have unique spectral, directional, and polarimetric properties. Samples designed and fabricated for infrared (IR) wavelengths have been characterized using broadband instruments to measure specular polarimetric transmittance or reflectance as in ellipsometry or integrated hemisphere transmittance or reflectance. We have developed a wavelength-tunable IR Mueller-matrix (Mm) polarimetric scatterometer which uses tunable external-cavity quantum-cascade lasers (EC-QCLs) to tune onto and off of the narrowband spectral resonances of nanostructured optical materials and performed full polarimeteric and directional evaluation to more fully characterize their behavior. Using a series of EC-QCLs, the instrument is tunable over 4.37-6.54 ÎŒm wavelengths in the mid-wave IR and 7.41-9.71 ÎŒm in the long-wave IR and makes measurements both at specular angles, acting as a Mm polarimeter, and at off-specular angles, acting as a Mm scatterometer. Example measurements of an IR thermal metamaterial are shown
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