Comparison of high -performance thin layer chromatography/UV-densitometry and UV-derivative spectrophotometry for determination of trimetazidine in pharmaceutical formulation

New methods for assaying trimetazidine dihydrochloride on the basis of thin layer chromatography and spectrophotometry are proposed and compared in the paper. In HPTLC/UV-densitometry, separation is achieved by using a mobile phase composed of ammonia-methanol (30:70, V/V) on silica gel HPTLC plates F254. Quantification using a non-linear calibration curve is accomplished by densitometric detection at 230 nm. Derivative spectrophotometric determination of trimetazidine dihydrochloride is carried out from the fourth derivative of the absorbance at 233 nm in peak-zero mode. Statistical comparison led to the conclusion that there is no significant difference between the two studied methods and, moreover, that they demonstrate satisfactory accuracy and precision for routine applications

Listy frekwencyjne dzienników ogólnopolskich w 2008 roku – raport badawczy

W 1972 r. nestor polskiego medioznawstwa prof. Walery Pisarek wydał książkę pt. Frekwencja wyrazów w prasie: wiadomości, komentarze, reportaże. Natomiast nie przeprowadzono analogicznego ba-dania frekwencji wyrazów dla polskiej prasy po 1989 r. W prezentowanym raporcie badawczym członkowie Koła Naukowego Obserwacji Polskich Mediów im. Stefana Kisielewskiego na Uniwersytecie Warszawskim przedstawiają wyniki projektubadawczego, dotyczącego używanych wyrazów w działach krajowych ogólnopolskiej, prestiżowej prasy codziennej (ang. broadsheet) w 2008 r. Wyniki list frekwencyjnych współczesnej prasy zestawione w tabele i wykresy pozwalają nie tylko wiarygodnie opisać język, jakim posługują się dzienniki, budującprzed oczyma swoich czytelników określoną narrację świata przedstawianego, ale również postawić zajmującą diagnozę dyskursu medialnego, w którymprestiżowe gazety odgrywają przemożną rolę.TOMASZ Gackowsk

Assessment of left ventricle diastolic function in myocardial infarction patients treated with primary angioplasty

Background: More than 5% of patients over the age of 65 have been found to develop heart failure, and over half of them preserve normal left ventricular systolic function. In the last few years, diastolic heart failure has become a serious clinical and epidemiological problem. Methods: This prospective study was carried out on 99 patients. Patients were evaluated up to three months after primary percutaneous coronary intervention (PCI). Evaluation was performed three times: within the first 24 hours after primary PCI, on day seven after PCI and at three months after PCI. We analyzed the relationship between the severity of left ventricle diastolic dysfunction and atherosclerosis risk factors, infarction site, maximum levels of cardiac biomarkers such as troponin I, CPK and CK-MB, CRP protein levels, angioplasty effectiveness, reperfusion time, TIMI and TMPG grade. Results: Most patients with ST elevation myocardial infarction (STEMI) treated with primary PCI demonstrated left ventricle diastolic dysfunction on the first day of myocardial infarction. Levels of cardiac biomarkers were significantly higher in patients with restrictive filling pattern. The inflammatory response (CRP levels) was found to have an important role in the development of diastolic abnormalities. There was a close relationship between diastolic and systolic function. Average values of left ventricular ejection fraction in patients with restrictive filling pattern were significantly lower than in those with impaired relaxation (44.7 vs. 52.7%; p < 0.001) and normal filling (54.2%; p = 0.002). Conclusions: More than half of patients with a first STEMI have left ventricle diastolic dysfunction within the first day after PCI, and these abnormalities are still present three months after PCI. Time and effectiveness of reperfusion, CRP level, troponin I, max, CPK max and CK-MB max levels as well as left ventricular ejection fraction have an important influence on the development of diastolic dysfunction. Infarct extension contributes significantly to the process

Medications of medieval monastery medicine

Herbal medicine has accompanied man since the dawn of time. For various ailments and health problems, help was sought in the surrounding nature. Ancient civilizations have greatly contributed to the development of phytotherapy, identifying and describing numerous species of medicinal plants. Plant raw materials have become the foundation of medieval medicine. The revival in herbal medicine has been observed since the sixth century with the emergence of monasteries, where the gardens in which the medicinal plants were grown were established. Monastic schools were also established, monks broadened their skills by studying and copying ancient books, preparing medicines of natural origin according to secret prescriptions used to heal the sick. Although the Middle Ages are often considered dark ages, many achievements of the then phytotherapy have been recorded on the pages of history and a man use them to this day

Systemic oxidoreductive balance in patients without clinical manifestation of atherosclerosis

Introduction. Oxidative stress plays an important role in atherosclerosis, but numerous clinical trials have not confirmed a favourable effect of antioxidant supplementation. We aimed to determine the oxidative stress parameters in patients without clinical manifestation of vascular disease. Material and methods. Forty-eight patients were divided into two groups in relation to the presence or absence of clinically silent signs of atherosclerosis (ankle-brachial index &lt; 0.9, intima-media thickness ≥ 0.9 mm, the presence of carotid atherosclerotic plaques, silent ischaemia in a treadmill stress test or focal myocardial contractility found in echocardiography). Plasma concentrations of: retinol, ascorbic acid, alpha-tocopherol and uric acid, as well as the products of oxidative DNA damage repair: 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-oxodG) in blood leucocytes and urine, and 8-oxo-7,8-dihydroguanine (8-oxo-Gua) in urine. Results. Patients with signs of subclinical atherosclerosis had lower blood concentration of alpha- tocopherol, and a non-significantly greater urine concentration of 8-oxoGua. Women had significantly greater blood concentration of ascorbic acid and alpha-tocopherol, as well as lower level of retinol and uric acid. They also had greater leucocyte concentration of 8-oxodG. Plasma concentration of alpha-tocopherol 30.34 μM distinguished patients with and without signs of subclinical atherosclerosis. Conclusions. Oxidative stress has clinical importance in the early stages of atherosclerosis and may be helpful in predicting its subclinical stage. Women had higher level of antioxidant defence, which explains their natural protection against early atherosclerosis development. Further studies are needed to determine the usefulness of tocopherol determination as a biomarker for atherosclerosis risk evaluation

A massive gross haematuria in an 85-year-old patient with acquired haemophilia A

The authors herein present a case report of an 85-year-old female patient with massive gross haematuria in the course of acquired haemophilia A. Acquired haemophilia in the geriatric population is a rare but severe bleeding disorder. It is caused by suddenly appearing autoantibodies that interfere with coagulation factor VIII activity. The diagnosis of acquired haemophilia should be considered in any patient who presents with bleeding and a prolonged activated partial thromboplastin time (APTT)

Assessment of the prevalence of nootropic drugs by elderly patients in geriatric practice

So-called nootropics include a diverse group of drugs whose idea is to improve brain function. They are quite commonly accepted among the elderly. Although currently available data from the literature do not show unequivocally the efficacy of these drugs, in practice a large number of patients are in practice. These drugs are prescribed to patients fairly commonly and the range of indications for their use is wide and non-specific. This publication presents the mechanism of action of these nootropic drugs, historical outline of their use and epidemiological data on their use in elderly patients on the basis of patients treated in the Department of Geriatrics of Medical University in Bydgoszcz

Polski rynek kanałów zdelokalizowanych - analiza zjawiska i rekomendacje

W grudniu 2012 r. Departament Strategii Krajowej Rady Radiofonii i Telewizji wraz z firmą badawczą MTReserch przygotował pierwszą w Polsce, kompleksową analizę programów polskojęzycznych rozpowszechnianych z terytorium innych państw, a skierowanych na rynek polski - do polskiego widza. Temat analizy brzmiał "Polski rynek kanałów zdelokalizowanych - analiza zjawiska i rekomendacje", Analiza Biura KRRiT nr 13/2012 grudzień 2012 r. Określenie charakteru i skali zjawiska delokalizacji treści na polskim rynku audiowizualnych usług medialnych; Stworzenie aktualnej listy kanałów „zdelokalizowanych” dostępnych na rynku polskim oraz wskazanie samych podmiotów właścicielskich (nadawców) i miejsca ich zarejestrowania; Stworzenie kompletnej typologii kanałów „zdelokalizowanych” na polskim rynku ze względu na miejsce ich zarejestrowania, właściciela (nadawcę), sposób dystrybucji, krąg kulturowy oraz tematykę (gatunek); Dokonanie analizy 20 proc. kanałów „zdelokalizowanych” o największych udziałach w polskim rynku telewizyjnym pod kątem ich jakości, poruszanej tematyki oraz projektowanej widowni (wydźwięku programu i sposobu jego postrzegania). Dokonanie analizy dostępnej, anglojęzycznej dokumentacji dotyczącej sposobu funkcjonowania rynku linearnych, audiowizualnych usług medialnych oraz aktywności regulatorów w zakresie m. in. pluralizacji oferty programowej, podnoszenia jej jakości oraz zachęcania nadawców do starań o koncesję (ograniczanie efektu delokalizacji) w wybranych krajach Unii Europejskiej istotnych z polskiej perspektywy – Republika Czeska, Wielka Brytania, Szwecja, Niemcy oraz Francja; Przygotowanie rekomendacji działań, mających na celu ograniczenie negatywnych efektów zjawiska delokalizacji treści na polskim rynku linearnych audiowizualnym usług medialnych i zachęcenie zagranicznych nadawców do rejestrowania swoich programów w Polsce.TOMASZ GackowskiKrajowa Rada Radiofonii i Telewizj

Pulmonary artery dilatation during normal pregnancy

Background: During pregnancy the cardiovascular system undergoes complex alterations; however, there have been few studies concerning pulmonary artery behaviour during gestation. Aim: The aim of this study was to evaluate changes in the pulmonary artery diameter (PAD) during gestation, with respect to left ventricular (LV) load and remodelling, as well as to systemic vascular resistance (SVR). Methods: It was a prospective cohort study, which included 69 consecutive healthy outpatient women with a singleton pregnancy. Echocardiography and ultrasonography of the uterine arteries were performed at two visits (V1 and V2) during pregnancy, and only echocardiography at one visit postpartum. Results: Pulmonary artery diameter increased in all women, from 20.0 mm at V1 to 22.7 mm at V2 (p &lt; 0.001), and it returned to the reference range postpartum (19.5 mm; p &lt; 0.001 vs. V2). Echocardiography did not reveal any other abnormalities, including the signs of intracardiac shunt or right ventricular pressure overload. During V2, PAD positively correlated with the following echocardiographic parameters: LV end-diastolic diameter (r = 0.386; p = 0.002), LV end-systolic diameter (r = 0.345 p = 0.006), LV end-diastolic volume (r = 0.308; p = 0.016), LV mass (r = 0.459; p &lt; 0.001), left atrial area (r = 0.334; p = 0.009), LV cardiac output (r = 0.338; p = 0.011), and aortic diameter (r = 0.369; p = 0.003). Furthermore, there was a negative linear correlation between PAD and SVR (r = –0.307; p = 0.025) and pulsatility index (r = –0.318; p = 0.012). Conclusions: Our study shows pulmonary artery dilatation in healthy pregnant women without any other abnormal echocardiographic findings. These changes in PAD are associated with volume overload and low vascular resistance, and during postpartum they usually subside