Application of the seven-factor-model of personality to an Italian preschool sample

OBJECTIVE: Advances in dimensional assessment of children in healthy and clinical populations has renewed interest in the study of temperament. Cloninger's Temperament and Character Inventory (TCI) has shown high reliability and internal consistency. Adult and adolescent versions have been translated into a number of languages and validated in cross-cultural studies worldwide. To date only one preschool-TCI-based study has been conducted in early infancy with teachers as observers. The present study is aimed to test an Italian Preschool version of the Temperament and Character Inventory (PsTCI). This is the first replication and the first validation study of TCI on preschoolers with parents as observers. METHODS: 395 preschool children, recruited from pediatric communities and day-care centres throughout Italy, participated in the study. Parents of each child enrolled in the study and completed a PsTCI about the child. Standard psychometric tests of reliability and validation were performed. RESULTS: Exploratory factor analyses demonstrated the presence of distinct domains for temperament and character. TCI dimensions had good internal consistency with Cronbach's alpha ranging values (|0.60|-|0.81|). Gender differences were found for Harm Avoidance (β=-0.186; p≤0.001) and Self-Directedness (β=-0.216; p≤0.01), and accounted for 5-35arm-38-702- of the observed variance. CONCLUSION: The present work suggests the psychological complexity of Cloninger's model and confirms its application in pre-school children from diverse environmental and cultural backgrounds. The results confirm that Cloninger's instrument for temperament and character evaluations can also be used with different observers and highlight the importance of considering cultural and demographic differences in the assessment of temperament and character in preschoolers

Resistance to citrus canker induced by a variant of Xanthomonas citri ssp. citri is associated with a hypersensitive cell death response involving autophagy-associated vacuolar processes

[EN] Xanthomonas citri ssp. citri (X. citri) is the causal agent of Asiatic citrus canker, a disease that seriously affects most commercially important Citrus species worldwide. We have identified previously a natural variant, X. citri A(T), that triggers a host-specific defence response in Citrus limon. However, the mechanisms involved in this canker disease resistance are unknown. In this work, the defence response induced by X. citri A(T) was assessed by transcriptomic, physiological and ultrastructural analyses, and the effects on bacterial biofilm formation were monitored in parallel. We show that X. citri A(T) triggers a hypersensitive response associated with the interference of biofilm development and arrest of bacterial growth in C. limon. This plant response involves an extensive transcriptional reprogramming, setting in motion cell wall reinforcement, the oxidative burst and the accumulation of salicylic acid (SA) and phenolic compounds. Ultrastructural analyses revealed subcellular changes involving the activation of autophagy-associated vacuolar processes. Our findings show the activation of SA-dependent defence in response to X. citri A(T) and suggest a coordinated regulation between the SA and flavonoid pathways, which is associated with autophagy mechanisms that control pathogen invasion in C. limon. Furthermore, this defence response protects C. limon plants from disease on subsequent challenges by pathogenic X. citri. This knowledge will allow the rational exploitation of the plant immune system as a biotechnological approach for the management of the disease.This work was principally supported by the Agencia Nacional de Promocion Cientifica y Tecnologica (PICT-2011-1833) to M.R.M. and by a grant from the Florida Citrus Research and Development Foundation to F.G.G. and M.R.M. 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PthA4AT, a 7.5-repeats transcription activator-like (TAL) effector from Xanthomonas citri ssp. citri, triggers citrus canker resistance

Transcription activator-like effectors (TALEs) are important effectors of Xanthomonas spp. that manipulate the transcriptome of the host plant, conferring susceptibility or resistance to bacterial infection. Xanthomonas citri ssp. citri variant AT (X. citri AT) triggers a host-specific hypersensitive response (HR) that suppresses citrus canker development. However, the bacterial effector that elicits this process is unknown. In this study, we show that a 7.5-repeat TALE is responsible for triggering the HR. PthA4AT was identified within the pthA repertoire of X. citri AT followed by assay of the effects on different hosts. The mode of action of PthA4AT was characterized using protein-binding microarrays and testing the effects of deletion of the nuclear localization signals and activation domain on plant responses. PthA4AT is able to bind DNA and activate transcription in an effector binding element-dependent manner. Moreover, HR requires PthA4AT nuclear localization, suggesting the activation of executor resistance (R) genes in host and non-host plants. This is the first case where a TALE of unusually short length performs a biological function by means of its repeat domain, indicating that the action of these effectors to reprogramme the host transcriptome following nuclear localization is not limited to ‘classical’ TALEs.Fil: Roeschlin, Roxana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Uviedo, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: García, Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Molina, María Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Favaro, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Chiesa, Maria Amalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Tasselli, Sabrina Emilse. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Franco Zorrilla, José Manuel. Instituto de Bioquímica y Biología Molecular; ArgentinaFil: Forment, Javier. Universidad Politécnica de Valencia; EspañaFil: Gadea, José. Universidad Politécnica de Valencia; EspañaFil: Marano, María Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentin

Role of xanthan in biofilm formation during Xanthomonas citri subsp. citri epiphytic life and its relationship to canker development

La mayoría de las bacterias fitopatógenas viven de forma epifítica sobre las plantas antes de la colonización y sobreviven en la superficie del tejido del hospedante, mediante la formación de biopelículas. La capacidad para producir exopolisacáridos es crítica para la formación de las mismas en diversas bacterias. En este trabajo, se estudió la formación de biopelículas de X. citri subsp. citri, bacteria responsable de la cancrosis de los cítricos, y su relación con la supervivencia epifítica y el desarrollo del cancro sobre hojas de plantas de limonero. Mediante tinción con cristal violeta y análisis de microscopía confocal de barrido láser, se observó que X. citri subsp. citri forma estructuras de biopelículas sobre hojas de limonero. En contraste, una cepa mutante defectiva en la biosíntesis del exopolisacárido xantano, denominada X. citri subsp. citri gumB, fue incapaz de formar dichas estructuras y su supervivencia epifítica y capacidad de desarrollar la enfermedad en la planta se vieron comprometidas. Estos resultados sugieren que la formación de biopelículas tiene una importancia clave en la fase epifítica de X. citri subsp. citri antes y durante el desarrollo del cancro.Almost all phytopathogenic bacteria have an epiphytic life before invading the host plant. These bacteria survive on the surface of the host tissue through biofilm formation. The ability to produce exopolysaccharides is critical for biofilm formation in several phytopathogenic bacteria. In this work, biofilm formation by Xanthomonas citri subsp. citri (bacterium responsible for citrus canker disease), and its relationship to epiphytic survival and canker development on lemon leaves were studied. Through crystal violet staining and confocal laser scanning microscopy analysis, it was observed that X. citri subsp. citri forms biofilm structures on lemon leaves. Furthermore, a X. citri subsp. citri gumB mutant strain, defective in production of exopolysaccharide xanthan, did not form a structured biofilm and showed reduced growth and survival on leaf surfaces, and reduced disease symptoms. These results suggest that biofilm formation has an important role in X. citri subsp. citri epiphytic survival prior to canker development.Fil: Rigano, Luciano Ariel. Fundacion Pablo Cassara; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; ArgentinaFil: Siciliano, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Enrique, Ramón Atanasio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Sendín, Lorena Noelia. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino; Argentina; ArgentinaFil: Filippone, María Paula. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino; Argentina; ArgentinaFil: Torres, Pablo Sebastian. Fundacion Pablo Cassara; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; ArgentinaFil: Qüesta, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Dow, J. Maxwell. National University of Ireland; IrlandaFil: Castagnaro, Atilio Pedro. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino; Argentina; ArgentinaFil: Vojnov, Adrian Alberto. Fundacion Pablo Cassara; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; ArgentinaFil: Marano, Maria Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentin

The innovative Cherenkov camera based on SiPM sensors of the ASTRI-Horn telescope: from the T/M and electrical design to the full assembly and testing in a harsh environment

ASTRI-Horn is a prototypal telescope of an imaging atmospheric Cherenkov telescope developed by the Italian National Institute of Astrophysics (INAF), proposed for the Cherenkov Telescope Array (CTA) Observatory. The CTA Observatory represents the next generation of imaging atmospheric Cherenkov telescopes and will explore the very highenergy domain from a few tens of GeV up to few hundreds of TeV. It will be composed of large-, medium-, and small sized telescopes; ASTRI-Horn is an end-to-end prototype proposed for the Small Sized array. The main scientific instrument of the ASTRI-Horn telescope is an innovative and compact Camera with Silicon- Photomultiplier based detectors and a specifically designed fast read-out electronics based on a custom peak-detector mode. The thermo-mechanical assembly is designed to host both the entire electronics chain, from the sensors to the raw data transmission system and the calibration system, and the complete thermoregulation system. This contribution gives a high level description of the T/M and electrical design of the Cherenkov Camera, it describes the assembling procedure of its different subsystems and their integration into the complete camera system. A discussion about possible design improvements coming from the problems/difficulties encountered during assembly is also presented. Finally, results from engineering tests conducted in-field are also presented

The ASTRI camera for the Cherenkov Telescope Array

The Cherenkov Telescope Array (CTA) foresees, in its southern site (Chile), the implementation of up to 70 small-sized telescopes (SSTs), which will extend the energy coverage up to hundreds of TeV. It has been proposed that one of the first set of CTA SSTs will be represented by the ASTRI mini-array, which includes (at least) nine ASTRI telescopes. The endto-end prototype of such telescopes, named the ASTRI SST-2M, is installed in Italy and it is now completing the overall commissioning and entering the science verification phase. ASTRI telescopes are characterized by an optical system based on a dual-mirror Schwarzschild-Couder design and a camera at the focal plane composed of silicon photomultiplier sensors managed by a fast read-out electronics specifically designed. Based on a custom peak-detector mode, the ASTRI camera electronics is designed to perform Cherenkov signal detection, trigger generation, digital conversion of the signals and data transmission to the camera server. In this contribution we will describe the main features of the ASTRI camera, its performance and results obtained during the commissioning phase of the ASTRI SST-2M prototype in view of the ASTRI mini-array implementation

Post-intervention Status in Patients With Refractory Myasthenia Gravis Treated With Eculizumab During REGAIN and Its Open-Label Extension

OBJECTIVE: To evaluate whether eculizumab helps patients with anti-acetylcholine receptor-positive (AChR+) refractory generalized myasthenia gravis (gMG) achieve the Myasthenia Gravis Foundation of America (MGFA) post-intervention status of minimal manifestations (MM), we assessed patients' status throughout REGAIN (Safety and Efficacy of Eculizumab in AChR+ Refractory Generalized Myasthenia Gravis) and its open-label extension. METHODS: Patients who completed the REGAIN randomized controlled trial and continued into the open-label extension were included in this tertiary endpoint analysis. Patients were assessed for the MGFA post-intervention status of improved, unchanged, worse, MM, and pharmacologic remission at defined time points during REGAIN and through week 130 of the open-label study. RESULTS: A total of 117 patients completed REGAIN and continued into the open-label study (eculizumab/eculizumab: 56; placebo/eculizumab: 61). At week 26 of REGAIN, more eculizumab-treated patients than placebo-treated patients achieved a status of improved (60.7% vs 41.7%) or MM (25.0% vs 13.3%; common OR: 2.3; 95% CI: 1.1-4.5). After 130 weeks of eculizumab treatment, 88.0% of patients achieved improved status and 57.3% of patients achieved MM status. The safety profile of eculizumab was consistent with its known profile and no new safety signals were detected. CONCLUSION: Eculizumab led to rapid and sustained achievement of MM in patients with AChR+ refractory gMG. These findings support the use of eculizumab in this previously difficult-to-treat patient population. CLINICALTRIALSGOV IDENTIFIER: REGAIN, NCT01997229; REGAIN open-label extension, NCT02301624. CLASSIFICATION OF EVIDENCE: This study provides Class II evidence that, after 26 weeks of eculizumab treatment, 25.0% of adults with AChR+ refractory gMG achieved MM, compared with 13.3% who received placebo

Minimal Symptom Expression' in Patients With Acetylcholine Receptor Antibody-Positive Refractory Generalized Myasthenia Gravis Treated With Eculizumab

The efficacy and tolerability of eculizumab were assessed in REGAIN, a 26-week, phase 3, randomized, double-blind, placebo-controlled study in anti-acetylcholine receptor antibody-positive (AChR+) refractory generalized myasthenia gravis (gMG), and its open-label extension