Džibra kao izvor biofaktora promocije rasta i stimulator sinteze levana i ekstracelularne levansaharoze u Zymomonas mobilis

In the present work, the fermentation of simultaneous production of ethanol and levan by Zymomonas mobilis grown on different growth media has been studied. Yeast extract, rye stillage or sugar beet molasses stillage were used as additives to the basic sucrose media and the chemical composition, including vitamins, of the cultivation liquids have been determined. It has been shown that 0.5 % of yeast extract dry weight additive could be substituted by 10.0 % of native stillage additive. It was established that molasses stillage stimulates the ethanol synthesis, but rye stillage additive is more preferable for levan production. The extracellular levansucrase obtained from the culture liquid resulted in similar fructooligosaccharide-producing activities using all the above-mentioned media additives.U radu je istraživana istodobna proizvodnja etanola i levana fermentacijom Z. mobilis na raznim podlogama. Ekstrakt kvasca, džibra od rižine melase ili melase šećerne repe upotrijebljeni su kao dodaci osnovnoj šećernoj otopini, a određen je kemijski sastav podloge za uzgoj, uključujući vitamine. Pokazalo se da se 0,5 % udjela suhe tvari kvaščeva ekstrakta kao aditiva može zamijeniti s 10,0 % izvorne džibre. Utvrđeno je da melasna džibra stimulira sintezu etanola, a džibra je rižine melase povoljnija za proizvodnju levana. Ekstracelularna levansaharoza dobivena iz podloge za uzgoj, koristeći sve navedene dodatke, pokazala je sličnu aktivnost proizvodnje fruktooligosaharida

Metabolic Profiling of Glucose-Fed Metabolically Active Resting Zymomonas mobilis Strains

Zymomonas mobilis is the most efficient bacterial ethanol producer and its physiology is potentially applicable to industrial-scale bioethanol production. However, compared to other industrially important microorganisms, the Z. mobilis metabolome and adaptation to various nutritional and genetic perturbations have been poorly characterized. For rational metabolic engineering, it is essential to understand how central metabolism and intracellular redox balance are maintained in Z. mobilis under various conditions. In this study, we applied quantitative mass spectrometry-based metabolomics to explore how glucose-fed non-growing Z. mobilis Zm6 cells metabolically adapt to change of oxygen availability. Mutants partially impaired in ethanol synthesis (Zm6 adhB) or oxidative stress response (Zm6 cat) were also examined. Distinct patterns of adaptation of central metabolite pools due to the change in cultivation condition and between the mutants and Zm6 reference strain were observed. Decreased NADH/NAD ratio under aerobic incubation corresponded to higher concentrations of the phosphorylated glycolytic intermediates, in accordance with predictions of the kinetic model of Entner–Doudoroff pathway. The effects on the metabolite pools of aerobic to anaerobic transition were similar in the mutants, yet less pronounced. The present data on metabolic plasticity of non-growing Z. mobilis cells will facilitate the further metabolic engineering of the respective strains and their application as biocatalysts

Metabolic Profiling of Glucose-Fed Metabolically Active Resting Zymomonas mobilis Strains

Zymomonas mobilis is the most efficient bacterial ethanol producer and its physiology is potentially applicable to industrial-scale bioethanol production. However, compared to other industrially important microorganisms, the Z. mobilis metabolome and adaptation to various nutritional and genetic perturbations have been poorly characterized. For rational metabolic engineering, it is essential to understand how central metabolism and intracellular redox balance are maintained in Z. mobilis under various conditions. In this study, we applied quantitative mass spectrometry-based metabolomics to explore how glucose-fed non-growing Z. mobilis Zm6 cells metabolically adapt to change of oxygen availability. Mutants partially impaired in ethanol synthesis (Zm6 adhB) or oxidative stress response (Zm6 cat) were also examined. Distinct patterns of adaptation of central metabolite pools due to the change in cultivation condition and between the mutants and Zm6 reference strain were observed. Decreased NADH/NAD ratio under aerobic incubation corresponded to higher concentrations of the phosphorylated glycolytic intermediates, in accordance with predictions of the kinetic model of Entner–Doudoroff pathway. The effects on the metabolite pools of aerobic to anaerobic transition were similar in the mutants, yet less pronounced. The present data on metabolic plasticity of non-growing Z. mobilis cells will facilitate the further metabolic engineering of the respective strains and their application as biocatalysts

Oxidative Phosphorylation for Aerobic Survival, but Not for Growth: The Peculiar ‘Make-Accumulate-Consume’ Strategy in <i>Zymomonas mobilis</i>

Understanding the energy metabolism and its regulation is one of the clues to metabolic engineering of stress-resistant lignocellulose-converting microbial strains, also including the promising ethanologen Zymomonas mobilis. Z. mobilis is an obligately fermentative, facultatively anaerobic bacterium, carrying an active respiratory chain with low energy-coupling efficiency. Its respiration does not supply energy to aerobically growing cultures on sugary media, yet oxidative phosphorylation has been demonstrated in non-growing cells with ethanol. Here, we show, for the first time, that in respiring, non-growing Z. mobilis cells receiving regular small amounts of ethanol, oxidative phosphorylation significantly contributes to the maintenance of their viability. No improvement of viability is seen in the NADH dehydrogenase (ndh)-deficient respiratory mutant, which is unable to oxidize ethanol. The ethanol effect is also hampered by the protonophoric uncoupler CCCP, or the inhibitor of ATP synthase, DCCD. At higher concentrations (6% v/v), ethanol causes stress that slows down culture growth. By monitoring the activity of several respiratory gene promoters under ethanol stress with the green fluorescent protein reporter system, we demonstrate downregulation of these promoters, in particular the ndh promoter. We speculate that the decrease in respiratory chain activity in response to stress conditions mitigates the production of reactive oxygen species

Application of FT-IR Spectroscopy for Investigation of Pink Water Remediation by Pine Bark

This study demonstrates the application of FT-IR spectroscopy for investigating the remediation of pink water with the low cost adsorbent pine bark. The removal of 2,4,6-trinitrotoluene (TNT) from pink water by adsorption to pine bark was accompanied by a reduction in intensities of peaks at 1544 and 1347 cm-1 in the spectra of acetonitrile extracts of the pine bark. Hierarchial cluster analysis differentiated samples with high (30-180 mg/L) and low (0-4 mg/L) TNT concentrations, demonstrating the potential of this approach as a quick screening method for the control of the removal of TNT from pink water. The amount of lignin in pine bark was inversely proportional to the size of the pine bark particles, while the concentration of phenolic hydroxyl groups increases with increasing size of pine bark particles. FT-IR spectra showed that as well as TNT, pine bark can also adsorb nitramine explosives such as RDX and HMX.BIOE

Optimization of Nitrification Process by a Bacterial Consortium in the Submerged Biofiltration System with Ceramic Bead Carrier

Laboratory-scale solid phase submerged system was developed to study the process of ammoniumbiodegradation. Ceramic beads were found to be an appropriate carrier material for the attachment of thePNNbacterial consortium (Pseudomonas sp., Nitrosomonas sp., Nitrobacter sp.) exhibiting nitrification/denitrificationactivity. This consortium was previously isolated from a biological activated sludge process at a fish factorywastewater treatment plant. Three organic amendments - molasses, humic acid extract, and malt extract - were usedfor the ceramic bead pretreatment. Molasses significantly enhanced (p<0.05) the process of bacteria attachmentonto the ceramic carrier and further ammonium removal from the bulk liquid media. The addition of 0.45% fructoseto the column notably enhanced ammonium oxidation, as demonstrated by more rapid formation of nitrites in themedium when compared to the sets without sugars. The results of this study could be incorporated in a larger-scaletest of a biofiltration column using wastewater from a fish processing factor

Crypthecodinium cohnii Growth and Omega Fatty Acid Production in Mediums Supplemented with Extract from Recycled Biomass

Crypthecodinium cohnii is a marine heterotrophic dinoflagellate that can accumulate high amounts of omega-3 polyunsaturated fatty acids (PUFAs), and thus has the potential to replace conventional PUFAs production with eco-friendlier technology. So far, C. cohnii cultivation has been mainly carried out with the use of yeast extract (YE) as a nitrogen source. In the present study, alternative carbon and nitrogen sources were studied: the extraction ethanol (EE), remaining after lipid extraction, as a carbon source, and dinoflagellate extract (DE) from recycled algae biomass C. cohnii as a source of carbon, nitrogen, and vitamins. In mediums with glucose and DE, the highest specific biomass growth rate reached a maximum of 1.012 h&minus;1, while the biomass yield from substrate reached 0.601 g&middot;g&minus;1. EE as the carbon source, in comparison to pure ethanol, showed good results in terms of stimulating the biomass growth rate (an 18.5% increase in specific biomass growth rate was observed). DE supplement to the EE-based mediums promoted both the biomass growth (the specific growth rate reached 0.701 h&minus;1) and yield from the substrate (0.234 g&middot;g&minus;1). The FTIR spectroscopy data showed that mediums supplemented with EE or DE promoted the accumulation of PUFAs/docosahexaenoic acid (DHA), when compared to mediums containing glucose and commercial YE

Mikrobu biomasas analize ar infrasarkanas spektrofotometrijas metodi

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