Domestic water supply in rural Greenland:sufficiency, affordability and accessibility

Global efforts are still under way to ensure sustainable development goal 6 of providing enough clean water to sustain public health in many regions, and especially in the Arctic where the remoteness of communities and the harsh climate make water provision especially challenging. This study aimed to examine the sufficiency, accessibility, and affordability of water supplies in rural Greenland. The state of the water supply was investigated using quantitative data on infrastructure and demographics. Qualitative data on water-related practices and perceptions were collected through fieldwork and interviews in a selection of settlements. Generally, the supply of drinking water was found to be sufficient and affordable for most. However, access was severely constrained by the lack of piping to rural homes (20% were piped). The daily water consumption of residents from un-piped households was between 13 and 23 L/d/cap, i.e. within the basic access level according to WHO, which is in theory not sufficient to sustain public health. Several health risks could be caused by the low daily consumption in un-piped homes, and water saving practices induced by it – i.e. the use of shared handwashing basins, and household water storage, which could lead to degradation of water quality at the point-of-use

Managed groundwater recharge at the farm scale in pre-Saharan Morocco

The oases of the pre-Saharan basin of Wadi Ferkla in southeastern Morocco receives low and erratic rainfall (annual average of 141 mm and inter-annual standard deviation of 70 mm). From the 1980s, surface water and groundwater are increasingly used due to the expansion of irrigation, mainly along two wadis, namely Wadis Ferkla and Satt originating in the High-Atlas and the Anti-Atlas Mountains, respectively. Their flows reach the Ferkla's irrigated perimeters only when the volume of the flood events exceed upstream evaporation, withdrawals and riverbed's infiltration. Nowadays, these irrigated perimeters exert significant pressure on groundwater resources, through numerous drillings equipped with pumping systems, most of them being powered by solar energy. This increasing water demand situation incentivizes individual farmers to design and implement innovative techniques to increase water access for their farms. For instance, the spreading of floodwaters – an ancestral and collective irrigation practice in traditional oases – is currently being modernized by individual farmers. The new technique consists in partially diverting flood flows into earthen basins. The stored water either infiltrates to recharge local aquifers, or is pumped for flood irrigation of date palms. An experimental protocol was set up to characterize groundwater recharge below one of these on-farm basins equipped with a recharge well. Barometric probes were installed in the basin, in the recharge well and in neighboring boreholes to automatically monitor water levels. A topographic survey of the monitoring points and of the basin aimed at deriving piezometric levels from water levels measurements and estimating the height-surface-volume curves of the basin. After 7 months of continuous monitoring, 3 flood events were recorded. The establishment of the basin water balance at a fine time-resolution allowed estimating its different components including the infiltration rate influencing groundwater recharge. An analytical modeling of this process was developed to assess its effect on groundwater level variations. This approach aims to contribute to a broader reflection on securing water management in this fragile oasis ecosystem.</p

Determination of Matter Surface Distribution of Neutron-rich Nuclei

We demonstrate that the matter density distribution in the surface region is determined well by the use of the relatively low-intensity beams that become available at the upcoming radioactive beam facilities. Following the method used in the analyses of electron scattering, we examine how well the density distribution is determined in a model-independent way by generating pseudo data and by carefully applying statistical and systematic error analyses. We also study how the determination becomes deteriorated in the central region of the density, as the quality of data decreases. Determination of the density distributions of neutron-rich nuclei is performed by fixing parameters in the basis functions to the neighboring stable nuclei. The procedure allows that the knowledge of the density distributions of stable nuclei assists to strengthen the determination of their unstable isotopes.Comment: 41 pages, latex, 27 figure

Monitoring lactoferrin iron levels by fluorescence resonance energy transfer: A combined chemical and computational study

Three forms of lactoferrin (Lf) that differed in their levels of iron loading (Lf, LfFe, and LfFe2) were simultaneously labeled with the fluorophores AF350 and AF430. All three resulting fluorescent lactoferrins exhibited fluorescence resonance energy transfer (FRET), but they all presented different FRET patterns. Whereas only partial FRET was observed for Lf and LfFe, practically complete FRET was seen for the holo form (LfFe2). For each form of metal-loaded lactoferrin, the AF350–AF430 distance varied depending on the protein conformation, which in turn depended on the level of iron loading. Thus, the FRET patterns of these lactoferrins were found to correlate with their iron loading levels. In order to gain greater insight into the number of fluorophores and the different FRET patterns observed (i.e., their iron levels), a computational analysis was performed. The results highlighted a number of lysines that have the greatest influence on the FRET profile. Moreover, despite the lack of an X-ray structure for any LfFe species, our study also showed that this species presents modified subdomain organization of the N-lobe, which narrows its iron-binding site. Complete domain rearrangement occurs during the LfFe to LfFe2 transition. Finally, as an example of the possible applications of the results of this study, we made use of the FRET fingerprints of these fluorescent lactoferrins to monitor the interaction of lactoferrin with a healthy bacterium, namely Bifidobacterium breve. This latter study demonstrated that lactoferrin supplies iron to this bacterium, and suggested that this process occurs with no protein internalization.This work was supported by MINECO and FEDER (projects CTQ2012-32236, CTQ2011-23336, and BIO2012-39682-C02-02) and BIOSEARCH SA. F.C. and V.M.R. are grateful to the Spanish MINECO for FPI fellowships

Virtual coupling potential for elastic scattering of 10,11^{10,11}Be on proton and carbon targets

International audienceThe 10;11Be(p,p) and (12C, 12C) reactions were analyzed to determine the in uence of the weak binding energies of exotic nuclei on their interaction potential. The elastic cross sections were measured at GANIL in inverse kinematics using radioactive 10;11Be beams produced at energies of 39:1 A and 38:4A MeV. The elastic proton scattering data were analyzed within the framework of the microscopic Jeukenne-Lejeune-Mahaux (JLM) nucleon-nucleus potential. The angular distributions are found to be best reproduced by reducing the real part of the microscopic optical potential, as a consequence of the coupling to the continuum. These effects modify deeply the elastic potential. Including the Virtual Coupling Potential (VCP), we show the ability of the general optical potentials to reproduce the data for scattering of unstable nuclei, using realistic densities. Finally, the concepts needed to develop a more general and microscopic approach of the VCP are discussed

The \u3cem\u3eChlamydomonas\u3c/em\u3e Genome Reveals the Evolution of Key Animal and Plant Functions

Chlamydomonas reinhardtii is a unicellular green alga whose lineage diverged from land plants over 1 billion years ago. It is a model system for studying chloroplast-based photosynthesis, as well as the structure, assembly, and function of eukaryotic flagella (cilia), which were inherited from the common ancestor of plants and animals, but lost in land plants. We sequenced the ∼120-megabase nuclear genome of Chlamydomonas and performed comparative phylogenomic analyses, identifying genes encoding uncharacterized proteins that are likely associated with the function and biogenesis of chloroplasts or eukaryotic flagella. Analyses of the Chlamydomonas genome advance our understanding of the ancestral eukaryotic cell, reveal previously unknown genes associated with photosynthetic and flagellar functions, and establish links between ciliopathy and the composition and function of flagella

Breakdown of the Z = 8 Shell Closure in Unbound 12^{12}O and its Mirror Symmetry

Expérience GANIL, SISSI, MUST2/E537International audienceAn excited state in the proton-rich unbound nucleus 12O was identified at 1.8(4) MeV via missing-mass spectroscopy with the 14Oðp; tÞ reaction at 51 AMeV. The spin-parity of the state was determined to be 0þ or 2þ by comparing the measured differential cross sections with distorted-wave calculations. The lowered location of the excited state in 12O indicates the breakdown of the major shell closure at Z ¼ 8 near the proton drip line. This demonstrates the persistence of mirror symmetry in the disappearance of the magic number 8 between 12O and its mirror partner 12Be