Investigation of the anti-inflammatory and antinociceptive activities of Elephantorrhiza elephantina (Burch.) Skeels root extract in male rats

The anti-inflammatory and anti-nociceptive activities of the root extract of Elephantorrhiza elephantina (Burch.) Skeels (Fabaceae) were investigated using wistar rats. The extract was administered intraperitoneally (i.p) to rats at graded doses of 50, 100 and 200 mg/kg BWt. Carrageenan andHistamine were injected into rat paws sub-plantar to induce paw oedema, while acetic acid and formalin were injected i.p to induce pain. Indomethacin (10 mg/kg) was used as reference drug, whereas thevehicle [0.9% normal saline in 3% tween 80 (2 ml/kg)] was used as negative control. Acute toxicity was tested in rats at doses of 200, 400, 800 and 1600 mg/kg BWt. Compared to control, the aqueous extractof E. elephantina at all doses investigated significantly (P < 0.05) reduced the formation of oedema induced by Carrageenan and Histamine. The extract also caused a significant (P < 0.05) reduction in writhings in the acetic acid test and licking time in the formalin test. The rats did not show any signs of acute toxicity. The study revealed the anti-inflammatory and anti-nociceptive activities of the aqueous extract of E. elephantina, which may be due to the presence of phytochemical constituents such astannins and flavonoids. The acute toxicity test showed that the plant is relatively safe to use

Assessing water quality and its relationship to selected disease patterns in Zvishavane Town, Zimbabwe

The research intended to establish the relationship between Zvishavane domestic water quality and selected disease patterns. Total levels of coliform, Escherichia Coli, chemical and other physical parameter levels in samples from selected water sources during the period March 2018 to January 2019 were evaluated. Zimbabwe National Water Authority (ZINWA) commercial laboratory methods were used to analyze thirty one randomly selected sources. This quantitative research results were compared with recommended limits from Standard Association of Zimbabwe (SAZS 560:1997) and World Health Organization (WHO).Five water sources had coliform counts ranging from &lt; 1 to 55 cfu per 100 ml and Escherichia Coli from &lt; 1 to 28 cfu per 100 ml. Chloride levels of above 200 mg/L were obtained from eight borehole water sources. Levels of selected heavy metal ions were within the SAZS 560:1997 maximum allowable limits in all water samples. Sample levels for Mg2+ (29 %) and total hardness (32%) were above the maximum allowable levels. Tap water samples were within recommended limits for all measured parameters. Hypertension was first among the chronic disease conditions while diarrhoea and dysentery ranked second of the outpatient general diseases attended to at the Zvishavane District Hospital during 2018. 66 neonatal death were reported during the same time period. Zvishavane domestic water require research based pre-treatment methods before drinking. There is a possible link between the quality of water and the disease patterns. However these observations require further epidemiological studies to confirm the link.Keywords: Escherichia Coli, Hypertension, Diarrhoe

Anti-inflammatory and analgesic activities of the aqueous extract of Leonotis leonurus leaves in rats

Leonotis leonurus (L.) R. BR. Lamiaceae is extensively for the treatment of various ailments and in the Eastern Cape province of South Africa, it is used for the treatment of effects of gastrointestinal parasites in animals. There is, however, scanty information on the pharmacological activities of this plant. The aqueous extract from the leaf of L. leonurus was investigated for its analgesic and antiinflammatory properties. Carrageenan and histamine-induced rat paw oedema were conducted to evaluate anti-inflammatory activity, while acetic acid-induced writhing test was conducted to assess the analgesic activity of the plant. The extract was administered intraperitoneally (i.p) to rats at graded doses of 50, 100, 200 mg/kg body weight (BWt). Indomethacin (10 mg/kg BWt) was used as reference drug, whereas the vehicle (2 mg/kg BWt of 0.9% normal saline in Tween-80) was used as negative control. Acute toxicity was tested in rats at doses of 200, 400, 800 and 1600 mg/kg BWt. Compared with the control, the plant extract at 100 and 200 mg/kg BWt significantly (P &lt; 0.05) reduced the formation of carrageenan - induced oedema, while with histamine-induced oedema the difference was insignificant (P &gt; 0.05). In the acetic acid-induced writhing model, the plant extract produced a significant (P &lt; 0.05) reduction in the number of writhes with all test doses and at 100 and 200 mg/kg BWt, the extract produced results that were similar to those of Indomethacin. This study revealed the potential of L. leonurus leaf aqueous extract in reducing pain and inflammation, suggesting that it has some antiinflammatory and analgesic activities, hence, justifying its ethno-veterinary uses. The acute toxicity test showed that the plant is relatively safe to use.Key words: Analgesic, anti-inflammation, indomethacin, Leonotis leonurus, rats

Evaluation of carcass performance of Matebele goats managed extensively based on the eruption of permanent incisors teeth

Matebele goats were slaughtered at different state of permanent incisors eruption 1st pair (I1 )(237records), 2nd pair (I2 )(312 records), 3rd pair (I3 )(237records), 4th pair (I4)(232 ), Broken Mouth (BM)(214) and Smooth Mouth (SM)(218) over a period of 15 years (1989- 2004) and analysed for carcass performance. Performance parameters and weight of organs as a percentage of empty body weight (EBW) containing the effects of number of permanent incisors at I1, I2, I3, I4,, BM and SM were analysed using the General Linear Models. Live weight averaged 21.57 kg &#177; 0.02, 27.01&#177; 0.02 kg, 28.40&#177; 0.02 kg, 28.88&#177; 0.02 kg, 36.92&#177; 0.02 kg and 37.33&#177; 0.02 kg for goats slaughtered at I1, I2, I3, I4,,, BM and SM, respectively. There was a significant (p&lt;0.01) difference in slaughter weight at different periods of permanent incisors teeth eruptions. The goats slaughtered at I1 and I2 had significantly heavier hot carcass mass,cold carcass mass and rib barrel than those slaughtered I3, I4,, BM and SM. The goats slaughtered at I3 had the highest dressing percentage and goats slaughtered at BM had the lowest dressing percentage (DP). For external noncarcass components, the proportion of the head did not change but it was the feet proportion that increased markedly from I1 to I3 which coincided with the decrease in pelt. The proportion of hind barrel, head and kidney fat seemed to be constant. There was a significant (p&lt; 0.01) difference between mesenteric fat amongst teeth groups, which increased with increasing number of permanent incisors eruption representing age increase. The lower proportion of noncarcass components in I3 may have contributed to a higher DP

Meta-omics approaches to understand and improve wastewater treatment systems

Biological treatment of wastewaters depends on microbial processes, usually carried out by mixed microbial communities. Environmental and operational factors can affect microorganisms and/or impact microbial community function, and this has repercussion in bioreactor performance. Novel high-throughput molecular methods (metagenomics, metatranscriptomics, metaproteomics, metabolomics) are providing detailed knowledge on the microorganisms governing wastewater treatment systems and on their metabolic capabilities. The genomes of uncultured microbes with key roles in wastewater treatment plants (WWTP), such as the polyphosphate-accumulating microorganism Candidatus Accumulibacter phosphatis, the nitrite oxidizer Candidatus Nitrospira defluvii or the anammox bacterium Candidatus Kuenenia stuttgartiensis are now available through metagenomic studies. Metagenomics allows to genetically characterize full-scale WWTP and provides information on the lifestyles and physiology of key microorganisms for wastewater treatment. Integrating metagenomic data of microorganisms with metatranscriptomic, metaproteomic and metabolomic information provides a better understanding of the microbial responses to perturbations or environmental variations. Data integration may allow the creation of predictive behavior models of wastewater ecosystems, which could help in an improved exploitation of microbial processes. This review discusses the impact of meta-omic approaches on the understanding of wastewater treatment processes, and the implications of these methods for the optimization and design of wastewater treatment bioreactors.Research was supported by the Spanish Ministry of Education and Science (Contract Project CTQ2007-64324 and CONSOLIDER-CSD 2007-00055) and the Regional Government of Castilla y Leon (Ref. VA038A07). Research of AJMS is supported by the European Research Council (Grant 323009

Genetic dissection of the relationships between grain yield components by genome-wide association mapping in a collection of tetraploid wheats

Increasing grain yield potential in wheat has been a major target of most breeding programs. Genetic advance has been frequently hindered by negative correlations among yield components that have been often observed in segregant populations and germplasm collections. A tetraploid wheat collection was evaluated in seven environments and genotyped with a 90K SNP assay to identify major and stable quantitative trait loci (QTL) for grain yield per spike (GYS), kernel number per spike (KNS) and thousand-kernel weight (TKW), and to analyse the genetic relationships between the yield components at QTL level. The genome-wide association analysis detected eight, eleven and ten QTL for KNS, TKW and GYS, respectively, significant in at least three environments or two environments and the mean across environments. Most of the QTL for TKW and KNS were found located in different marker intervals, indicating that they are genetically controlled independently by each other. Out of eight KNS QTL, three were associated to significant increases of GYS, while the increased grain number of five additional QTL was completely or partially compensated by decreases in grain weight, thus producing no or reduced effects on GYS. Similarly, four consistent and five suggestive TKW QTL resulted in visible increase of GYS, while seven additional QTL were associated to reduced effects in grain number and no effects on GYS. Our results showed that QTL analysis for detecting TKW or KNS alleles useful for improving grain yield potential should consider the pleiotropic effects of the QTL or the association to other QTLs

Independent and combined effects of improved water, sanitation, and hygiene, and improved complementary feeding, on child stunting and anaemia in rural Zimbabwe: a cluster-randomised trial.

BACKGROUND: Child stunting reduces survival and impairs neurodevelopment. We tested the independent and combined effects of improved water, sanitation, and hygiene (WASH), and improved infant and young child feeding (IYCF) on stunting and anaemia in in Zimbabwe. METHODS: We did a cluster-randomised, community-based, 2 × 2 factorial trial in two rural districts in Zimbabwe. Clusters were defined as the catchment area of between one and four village health workers employed by the Zimbabwe Ministry of Health and Child Care. Women were eligible for inclusion if they permanently lived in clusters and were confirmed pregnant. Clusters were randomly assigned (1:1:1:1) to standard of care (52 clusters), IYCF (20 g of a small-quantity lipid-based nutrient supplement per day from age 6 to 18 months plus complementary feeding counselling; 53 clusters), WASH (construction of a ventilated improved pit latrine, provision of two handwashing stations, liquid soap, chlorine, and play space plus hygiene counselling; 53 clusters), or IYCF plus WASH (53 clusters). A constrained randomisation technique was used to achieve balance across the groups for 14 variables related to geography, demography, water access, and community-level sanitation coverage. Masking of participants and fieldworkers was not possible. The primary outcomes were infant length-for-age Z score and haemoglobin concentrations at 18 months of age among children born to mothers who were HIV negative during pregnancy. These outcomes were analysed in the intention-to-treat population. We estimated the effects of the interventions by comparing the two IYCF groups with the two non-IYCF groups and the two WASH groups with the two non-WASH groups, except for outcomes that had an important statistical interaction between the interventions. This trial is registered with ClinicalTrials.gov, number NCT01824940. FINDINGS: Between Nov 22, 2012, and March 27, 2015, 5280 pregnant women were enrolled from 211 clusters. 3686 children born to HIV-negative mothers were assessed at age 18 months (884 in the standard of care group from 52 clusters, 893 in the IYCF group from 53 clusters, 918 in the WASH group from 53 clusters, and 991 in the IYCF plus WASH group from 51 clusters). In the IYCF intervention groups, the mean length-for-age Z score was 0·16 (95% CI 0·08-0·23) higher and the mean haemoglobin concentration was 2·03 g/L (1·28-2·79) higher than those in the non-IYCF intervention groups. The IYCF intervention reduced the number of stunted children from 620 (35%) of 1792 to 514 (27%) of 1879, and the number of children with anaemia from 245 (13·9%) of 1759 to 193 (10·5%) of 1845. The WASH intervention had no effect on either primary outcome. Neither intervention reduced the prevalence of diarrhoea at 12 or 18 months. No trial-related serious adverse events, and only three trial-related adverse events, were reported. INTERPRETATION: Household-level elementary WASH interventions implemented in rural areas in low-income countries are unlikely to reduce stunting or anaemia and might not reduce diarrhoea. Implementation of these WASH interventions in combination with IYCF interventions is unlikely to reduce stunting or anaemia more than implementation of IYCF alone. FUNDING: Bill & Melinda Gates Foundation, UK Department for International Development, Wellcome Trust, Swiss Development Cooperation, UNICEF, and US National Institutes of Health.The SHINE trial is funded by the Bill & Melinda Gates Foundation (OPP1021542 and OPP113707); UK Department for International Development; Wellcome Trust, UK (093768/Z/10/Z, 108065/Z/15/Z and 203905/Z/16/Z); Swiss Agency for Development and Cooperation; US National Institutes of Health (2R01HD060338-06); and UNICEF (PCA-2017-0002)