17 research outputs found
Histamine regulates actin cytoskeleton in human toll-like receptor 4-activated monocyte-derived dendritic cells tuning CD4+ T lymphocyte response
Histamine, a major mediator in allergic diseases, differentially regulates the polarizing ability of dendritic cells after Toll-like receptor (TLR) stimulation, by not completely explained mechanisms. In this study we investigated the effects of histamine on innate immune reaction during the response of human monocyte-derived DCs (mDCs) to different TLR stimuli: LPS, specific for TLR4, and Pam3Cys, specific for heterodimer molecule TLR1/TLR2. We investigated actin remodeling induced by histamine together with mDCs phenotype, cytokine production, and the stimulatory and polarizing ability of Th0. By confocal microscopy and RT-PCR expression of Rac1/CdC42 Rho GTPases, responsible for actin remodeling, we show that histamine selectively modifies actin cytoskeleton organization induced by TLR4, but not TLR2 and this correlates with increased IL4 production and decreased IFNγ by primed T cells. We also demonstrate that histamine-induced cytoskeleton organization is at least in part mediated by down-regulation of small Rho GTPase CdC42 and the protein target PAK1, but not by down-regulation of Rac1. The presence and relative expression of histamine receptors HR1–4 and TLRs were determined as well. Independently of actin remodeling, histamine down-regulates IL12p70 and CXCL10 production in mDCs after TLR2 and TLR4 stimulation. We also observed a trend of IL10 up-regulation that, despite previous reports, did not reach statistical significance
BCA-1, A B-cell chemoattractant signal, is constantly expressed in cutaneous lymphoproliferative B-cell disorders.
CD30-CD30 ligand interaction in primary cutaneous CD30+ T-cell lymphomas: a clue to pathophysiology of clinical regression.
A novel allergen-adjuvant conjugate suitable for specific immunotherapy of respiratory allergy
High CD30 Ligand Expression by Epithelial Cells and Hassal's Corpuscles in the Medulla of Human Thymus
High CD30 Ligand Expression by Epithelial Cells and Hassal's Corpuscles in the Medulla of Human Thymus
Modified Adenine (9-Benzyl-2-Butoxy-8-Hydroxyadenine) Redirects Th2-Mediated Murine Lung Inflammation by Triggering TLR7
Carbon Nanotube Scaffolds Instruct Human Dendritic Cells: Modulating Immune Responses by Contacts at the Nanoscale
Nanomaterials interact with cells
and modify their function and
biology. Manufacturing this ability can provide tissue-engineering
scaffolds with nanostructures able to influence tissue growth and
performance. Carbon nanotube compatibility with biomolecules motivated
ongoing interest in the development of biosensors and devices including
such materials. More recently, carbon nanotubes have been applied
in several areas of nerve tissue engineering to study cell behavior
or to instruct the growth and organization of neural networks. To
gather further knowledge on the true potential of future constructs,
in particular to assess their immune-modulatory action, we evaluate
carbon nanotubes interactions with human dendritic cells (DCs). DCs
are professional antigen-presenting cells and their behavior can predict
immune responses triggered by adhesion-dependent signaling. Here,
we incorporate DC cultures to carbon nanotubes and we show by phenotype,
microscopy, and transcriptional analysis that in vitro differentiated
and activated DCs show when interfaced to carbon nanotubes a lower
immunogenic profile
Carbon Nanotube Scaffolds Instruct Human Dendritic Cells: Modulating Immune Responses by Contacts at the Nanoscale
Nanomaterials interact with cells
and modify their function and
biology. Manufacturing this ability can provide tissue-engineering
scaffolds with nanostructures able to influence tissue growth and
performance. Carbon nanotube compatibility with biomolecules motivated
ongoing interest in the development of biosensors and devices including
such materials. More recently, carbon nanotubes have been applied
in several areas of nerve tissue engineering to study cell behavior
or to instruct the growth and organization of neural networks. To
gather further knowledge on the true potential of future constructs,
in particular to assess their immune-modulatory action, we evaluate
carbon nanotubes interactions with human dendritic cells (DCs). DCs
are professional antigen-presenting cells and their behavior can predict
immune responses triggered by adhesion-dependent signaling. Here,
we incorporate DC cultures to carbon nanotubes and we show by phenotype,
microscopy, and transcriptional analysis that in vitro differentiated
and activated DCs show when interfaced to carbon nanotubes a lower
immunogenic profile