Dynamic Control of Dendritic mRNA Expression by CNOT7 Regulates Synaptic Efficacy and Higher Cognitive Function

Translation of mRNAs in dendrites mediates synaptic plasticity, the probable cellular basis of learning and memory. Coordination of translational inhibitory and stimulatory mechanisms, as well as dendritic transport of mRNA, is necessary to ensure proper control of this local translation. Here, we find that the deadenylase CNOT7 dynamically regulates dendritic mRNA translation and transport, as well as synaptic plasticity and higher cognitive function. In cultured hippocampal neurons, synaptic stimulation induces a rapid decrease in CNOT7, which, in the short-term, results in poly(A) tail lengthening of target mRNAs. However, at later times following stimulation, decreased poly(A) and dendritic localization of mRNA take place, similar to what is observed when CNOT7 is depleted over several days. In mice, CNOT7 is essential for hippocampal-dependent learning and memory. This study identifies CNOT7 as an important regulator of RNA transport and translation in dendrites, as well as higher cognitive function

Lawrence Livermore National Laboratory Safeguards and Security quarterly progress report ending March 31, 1996

LLNL carries out safeguards and security activities for DOE Office of Safeguards and Security (OSS) and other organizations within and outside DOE. LLNL is supporting OSS in 6 areas: safeguards technology, safeguards and materials accountability, computer security--distributed systems, complex-wide access control, standardization of security systems, and information technology and security center. This report describes the activities in each of these areas

Performa Fungi Mikoriza Arbuskula Dan Pueraria Phaseoloides Yang Dipupuk Tepung Tulang Dengan Ukuran Dan Dosis Berbeda

Bone meal is a natural source of calcium and phosphorus required for plant growth, but phosphorus can cause problems if they occur in high concentrations particularly will inhibit growth of mycorrhizal fungi. The aim of this study was to find the best diameter size and dosage of bone meal for increasing growth of forage legume Pueraria phaseoloides and for producing arbuscular mycorrhizal fungi (AMF) Glomus etunicatum inoculant. A glass house experiment was laid out in a randomized block design consisted of 13 treatments (control and 12 combination of different diameter size and weight of bone meal) and replicated three times. Bone meal and artificial fertilizer resulted the same effect (P>0.05) on dry matter of P. phaseoloides. Application of 25–40 mg with <250 µm diameter significantly (P<0.05) increased dry matter production. Bone meal reduced significantly (P<0.05) root colonization but increased significantly number of spores compared to that of artificial fertilizer. Reducing bone meal diameter significantly increased root colonization and number of spores of AMF G. etunicatum

Oceanographic drivers of population differentiation in Indo-Pacific bottlenose (Tursiops aduncus) and humpback (Sousa spp.) dolphins of the northern Bay of Bengal

The Bay of Bengal is one of the most productive ecosystems in the northern Indian Ocean and it harbours a rich community of cetaceans, including Indo-Pacific bottlenose (Tursiops aduncus) and humpback (Sousa spp.) dolphins. The taxonomy of these genera has been controversial, but within the Indian Ocean both seem to be divided into phylogenetically discrete units that range from the east to the west. Within the Sousa genus, S. plumbea is distributed in the western Indian Ocean while S. chinensis is distributed in the eastern Indian and western Pacific Ocean. T. aduncus has a discontinuous distribution throughout the Indo-Pacific Ocean and two different phylogenetic units are known to exist, one along the eastern African coast and another one in the eastern Indian and west Pacific Ocean. In this study we investigate the phylogeography of Indo-Pacific humpback and bottlenose dolphins in the northern Bay of Bengal. We sequenced the mitochondrial DNA control region for 17 bottlenose and 15 humpback dolphins and compared the results with previously published sequences within each genus. In both cases, we found that Bangladesh dolphins are genetically different from neighbouring populations. While the Bangladesh T. aduncus seem to be more closely related to the African T. aduncus form than the Pacific form, Sousa spp. seem to be more closely related to individuals from Australia. The genetic uniqueness of these populations has important evolutionary implications, due to their isolation, coastal distribution in a geographic cul-de-sac characterized by an extreme infusion, redistribution and recycling of biological productivity, and conservation implications since their survival is threatened in particular by fatal interactions with fisheries. We suggest that the particular and extreme oceanographic conditions found in the Bay of Bengal may be driving speciation in these dolphins and other marine megafauna.info:eu-repo/semantics/publishedVersio

CEREBROSPINAL FLUID DRAINAGE DEVICES: EXPERIMENTAL CARACTERIZATION

Hydrocephalus is a pathophysiology due to the excess of cerebrospinal fluid in the brain ventricles and it can be caused by congenital defects, brain abnormalities, tumors, inflammations, infections, intracranial hemorrhage and others. Hydrocephalus can be followed by significant rise of intraventricular pressure due to the excess of production of cerebrospinalfluid over the absorption, resulting in a weakening of intellectual functions, serious neurological damage (decreased movement, sensation and functions), critical physical disabilities and even death. A procedure for treatment involves the placement of a ventricular catheter into the cerebral ventricles to divert/drain the cerebrospinal fluid flow to a bag outside of the patient body – provisory treatment known as external ventricular drainage (EVD). Another option is the permanent treatment, internal ventricular drainage (IVD), promoting the cerebrospinal fluid drainage to other body cavity, being more commonly the abdominal cavity. In both cases, EVD and IVD, it is necessary to use of some type of neurological valve in order to control the flow of cerebrospinal fluid. In the present work is proposed an experimental procedure to test the hydrodynamic behavior of a complete drainage system, or parts of them, in order to verify its performance when subjected to pressure gradients found in the human body. Results show that the method is well adapted to quantify the pressure drop in neurological systems

Lawrence Livermore National Laboratory safeguards and security quarterly progress report to the U.S. Department of Energy. Quarter ending September 30, 1996

The paper describes tasks undertaken in each of the following areas: Safeguards technology program (STP); Safeguards and material accountability (SMA); Computer security, distributed systems; Complex-wide access control system (CWAC); and Standardization of security systems (SSS). The STP develops advanced, nondestructive analysis technology for measurement of special nuclear materials. Work focuses on R and D relating to X- and gamma-ray spectrometry and to development of computer codes for interpreting the spectral data obtained by these techniques. The SMA is concerned with four areas: insider protection; material accountability; planning and evaluation; and information security. The Computer Security Technology Center provides expertise and solutions to the many information security problems present in today`s computer systems and networks. Incidents of intrusions, computer viruses, the purposeful replacement of legitimate software for illegal purposes, and similar acts are being addressed by the creation of security software, the delivery of incident response expertise, and research and development into secure systems. The purpose of the CWAC is to develop an approach that will allow visitors to use their DOE standard badge in access control systems throughout the DOE complex. The purpose of the SSS project is to support the standardization of security systems to meet DOE orders and requirements, and to support the DOE in offering relevant security technology and capabilities to Federal standardization efforts

Lawrence Livermore National Laboratory safeguards and security quarterly progress report to the U.S. Department of Energy. Quarter ending September 30, 1996

The paper describes tasks undertaken in each of the following areas: Safeguards technology program (STP); Safeguards and material accountability (SMA); Computer security, distributed systems; Complex-wide access control system (CWAC); and Standardization of security systems (SSS). The STP develops advanced, nondestructive analysis technology for measurement of special nuclear materials. Work focuses on R and D relating to X- and gamma-ray spectrometry and to development of computer codes for interpreting the spectral data obtained by these techniques. The SMA is concerned with four areas: insider protection; material accountability; planning and evaluation; and information security. The Computer Security Technology Center provides expertise and solutions to the many information security problems present in today`s computer systems and networks. Incidents of intrusions, computer viruses, the purposeful replacement of legitimate software for illegal purposes, and similar acts are being addressed by the creation of security software, the delivery of incident response expertise, and research and development into secure systems. The purpose of the CWAC is to develop an approach that will allow visitors to use their DOE standard badge in access control systems throughout the DOE complex. The purpose of the SSS project is to support the standardization of security systems to meet DOE orders and requirements, and to support the DOE in offering relevant security technology and capabilities to Federal standardization efforts

Performa Fungi Mikoriza Arbuskula dan Pueraria phaseoloides yang Dipupuk Tepung Tulang dengan Ukuran dan Dosis Berbeda

Bone meal is a natural source of calcium and phosphorus required for plant growth, but phosphorus can cause problems if they occur in high concentrations particularly will inhibit growth of mycorrhizal fungi. The aim of this study was to find the best diameter size and dosage of bone meal for increasing growth of forage legume Pueraria phaseoloides and for producing arbuscular mycorrhizal fungi (AMF) Glomus etunicatum inoculant. A glass house experiment was laid out in a randomized block design consisted of 13 treatments (control and 12 combination of different diameter size and weight of bone meal) and replicated three times. Bone meal and artificial fertilizer resulted the same effect (P>0.05) on dry matter of P. phaseoloides. Application of 25–40 mg with <250 µm diameter significantly (P<0.05) increased dry matter production. Bone meal reduced significantly (P<0.05) root colonization but increased significantly number of spores compared to that of artificial fertilizer. Reducing bone meal diameter significantly increased root colonization and number of spores of AMF G. etunicatum

Calculations of radiation damage in target, container and window materials for spallation neutron sources

Radiation damage in target, container, and window materials for spallation neutron sources is am important factor in the design of target stations for accelerator-driver transmutation technologies. Calculations are described that use the LAHET and SPECTER codes to obtain displacement and helium production rates in tungsten, 316 stainless steel, and Inconel 718, which are major target, container, and window materials, respectively. Results are compared for the three materials, based on neutron spectra for NSNS and ATW spallation neutron sources, where the neutron fluxes are normalized to give the same flux of neutrons of all energies