Evaluation of pdx-1 gene expression in insulin producing cells, derived from embryonal carcinoma stem cells

زمینه و هدف: درک عملکرد سلول های بتا در سطح مولکولی به احتمال زیاد توسعه تکنیک های تولید سلول های بتا را تسهیل می کند. این مطالعه با هدف بررسی بیان ژن هومئوباکس دئودنال 1 (pdx-1) در سلول‌های تمایز یافته طراحی و اجرا شد. روش بررسی: این مطالعه بنیادی-کاربردی بر روی تمایز سلول‌های بنیادی به سلول‌های انسولین‌ساز انجام گرفت. محیط ثانویه حاصل از کشت پانکراس نوزاد یک هفته‌ای موش برای تمایز سلول‌های P19 استفاده شد. اجسام شبه جنینی (EBs) با کشت معلق 24 ساعته سلول‌های P19 تشکیل شدند. برای القای تمایز، غلظت‌های متفاوت محیط ثانویه (25، 50، 75 و 100) به محیط کشت اضافه شد. جهت شناسایی سلول‌های تمایز یافته مشتق از EBs در شرایط آزمایشــگاهی از رنگ آمیزی دیتیزون استفاده شد. تولید انسولین-پروانسولین و رسپتور بتای انسولین در این سلول ها به روش ایمنوفلورسنس تعیین و بیان ژن pdx-1 به وسیله واکنش زنجیره پلی‌مراز-رونویسی معکوس ارزیابی شد. داده ها با استفاده از آزمون های آماری آنالیز واریانس یک طرفه و دانکن تجزیه و تحلیل شدند. یافته‌ها: پس از هفت روز القا، دستجات سلولی تمایز یافته ظاهر شدند. اوج پاسخ‌گویی تمایزی مربوط به غلظت 50 از محیط ثانویه بود. بیان ژن pdx-1 در دستجات سلولی تمایز یافته مشاهده شد. بیان نشانگرهای انسولین-پروانسولین و رسپتور بتا در سلول‌های تمایز یافته به روش ایمنوفلورسنس اثبات شد. نتیجه‌گیری: محیط ثانویه پانکراس باعث تمایز سلول‌های P19به سلول‌های انسولین ساز ‌‌شد، لذا نتایج این مطالعه می تواند تولید سلول های بتا را از سلول های بنیادی تسهیل نماید

The Association Between Borna Disease Virus and Chronic Fatigue Syndrome: A Systematic Review and Meta-Analysis

Context: Several studies have controversial results regarding the association between Borna disease virus (BDV) and chronic fatigue syndrome (CFS). The present systematic review and meta-analysis investigated the association between CFS and BDV. Evidence Acquisition: The present study was conducted based on the PRISMA guidelines for systematic review and meta-analysis studies. To avoid bias, all procedures of the study were performed by two researchers independently. A comprehensive search was performed using online databases, including PubMed, Scopus, Embase, PsycINFO, science Direct, Cochrane Library, web of science, and Google scholar using the MeSH keywords until December 12th, 2016. Heterogeneity among studies was evaluated using Cochran's Q test I-2 Index. Finally, the random effect model was used for combined Odds Ratio (OR) using Review Manger software version 5.3.5. Results: Among 9 eligible studies comprising 610 cases and 2176 controls, the total OR for association between BDV and CFS was estimated to be 10.41 (95 confidence interval CI: 4.24 - 25.55, P < 0.0001). For subgroup analysis by the diagnostic methods (ELISA, RT-PCR, and Western blot), OR was 2.47 (95% CI: 0.77 - 7.96, P=0.13), 12.20 (95% CI: 1.66 - 89.57, P=0.01), and 28.36 (95% CI: 3.76- 213.85, P=0.001), respectively (test for subgroup differences: P=0.08; I-2=59.5). Subgroup analysis for BDV and CFS association by antigens and antibodies had an OR of 12.20 (1.66, 89.57) and 8.20 (3.32, 20.22), respectively (test for subgroup differences: P=0.72; I-2=0). Conclusions: This study supports the association between BDV and CFS and shows the role of viral agents in etiology of CFS. Therefore, viral agents may play a role in the etiology of neuropsychiatric disorders

Survey on the metazoan parasites in Neogobius fishes from southeastern part of the Caspian Sea

In this study a parasitological investigation on 238 samples of Neogobius species from the south-east of Caspian Sea was carried out. The samples including Neogobius melanostomus (35), N. fluviatilis pallasi (103), N. kessleri gorlap (70) and N. bathybius (30) were seasonally seined by beach net during one year (summer 2006-spring 2007), fixed in 10 % buffer formalinand transferred to the laboratory. From 231 of examined fishes 235 specimens were infected (97/6 %). The collected parasites were as follows: Hysterothylacium aduncum, Raphidascaris acus, Cucullanus sphaerocephalus, Dichelyne minutus, Raphidascaroides sp., Cystidicola sp,. Gyrodactylus sp., and Corynosoma strumosum. Hereby, N.melanostomus is introduced as a new host for Raphidascaroides sp., C. sphaerocephalus and Cystidicola sp.. N. fluviatilis pallasi and N. kessleri gorlap for C. sphaerocephalus, Cystidicola sp. and H .adunacum. N. bathybius for C. sphaerocephalus, Cystidicola sp., H. aduncum, E.excisus, R. acus and C. strumosum. Cystidicola sp. and H. adunacum were reported for the first time from Iran. Among the investigated fishes N. fluviatilis pallasi possessed the highest and N. bathybius the lowest variety in infection with various species of parasites. The results of this study indicated that there are significant relations between total length (T.L.) and weight in N. fluviatilis pallasi with the number of C. sphaerocephalus, D. minutus and E. excises. Also there are significant relations between total length and weight in N. melanostomus and between weights in N. kessleri gorlap with the number of C. sphaerocephalus

Prediction of strain localization during sheet metal forming using bifurcation analysis and Gurson- type damage - Complas XI

The strain localization phenomenon that may occur during sheet metal forming represents a major cause of defective parts produced in the industry. Several instability criteria have been developed in the literature to predict the occurrence of these instabilities. The proposed work aims to couple a Gurson-type model to the Rice’s localization criterion. The implementation of the modeling is achieved via a user subroutine (Umat) in Abaqus/std using a Runge-Kutta explicit integration scheme. Finally, we show the effectiveness of the proposed coupling for the prediction of the formability of stretched metal sheets

An FCM-based dynamic modelling of integrated project delivery implementation challenges in construction projects

Question: What are the Integrated Project Delivery Implementation challenges in construction projects, their interrelationships and their effects on the project time, cost and quality? Purpose: The Purpose of this study is applying an efficient method to determine the most important challenges to IPD implementation in construction industry, and also to evaluate the interrelationships among these challenges and their effects on the project time, cost, and quality. Research Method: This study models available Integrated Project Delivery challenges using a real case data, through applying Fuzzy Cognitive Mapping technique. Findings: Results show that contractual factors have the major influence compared with others. This shows the significance of paying attention to why project stakeholders must be integrated throughout the project life cycle since early contract documentation stage. Limitations/Implications: This study is limited to the case selected from Tehran of Iran. Value for authors: This study is significant due to identifying, classifying and determining the intensity of effects of IPD implementation challenges on cost, time, and quality of construction projects. It results in planning, resolving the challenges, enhancing the quality of constructions and lastly saving the construction cost and time

Pimecrolimus 1 percent cream and pulsed dye laser in treatment of a patient with reticular erythematous mucinosis syndrome

We report on the efficacy of twice daily application of pimecrolimus 1 percent cream in a 48-year-old woman with reticular erythematous mucinosis (REM) syndrome and compare its results with pulsed dye laser (PDL) on the other side of her chest and back. The patient was previously treated by hydroxychloroquine but only a fair response was observed. After application of 5 months of pimecrolimus, the lesions completely resolved and the result was comparable with the other side of her body treated by pulsed dye laser PDL. Topical pimecrolimus and pulsed dye laser appear to be effective and safe treatments for REM. © 2007 Dermatology Online Journal

Testing special relativity with geodetic VLBI

Geodetic Very Long Baseline Interferometry (VLBI) measures the group delay in the barycentric reference frame. As the Earth is orbiting around the Solar system barycentre with the velocity $V$ of 30 km/s, VLBI proves to be a handy tool to detect the subtle effects of the special and general relativity theory with a magnitude of $(V/\textrm{c})^2$. The theoretical correction for the second order terms reaches up to 300~ps, and it is implemented in the geodetic VLBI group delay model. The total contribution of the second order terms splits into two effects - the variation of the Earth scale, and the deflection of the apparent position of the radio source. The Robertson-Mansouri-Sexl (RMS) generalization of the Lorenz transformation is used for many modern tests of the special relativity theory. We develop an alteration of the RMS formalism to probe the Lorenz invariance with the geodetic VLBI data. The kinematic approach implies three parameters (as a function of the moving reference frame velocity) and the standard Einstein synchronisation. A generalised relativistic model of geodetic VLBI data includes all three parameters that could be estimated. Though, since the modern laboratory Michelson-Morley and Kennedy-Thorndike experiments are more accurate than VLBI technique, the presented equations may be used to test the VLBI group delay model itself.Comment: Proceedings of the IAG 2017 Scientific Meeting, Kobe, Japa

PNEUMATOSE KYSTIQUE INTESTINALE REVELEE PAR UNE ASCITE

La pneumatose kystique intestinale est une affection rare et curieuse, définie par la présence de kystes gazeux dans la paroi digestive, d'évolution habituellement bénigne. Sa connaissance est nécessaire afin d'éviter une inflation d'explorations et de mesures thérapeutiques dénuée de bénéfice pour le patient. Les auteurs rapportent l’observation d’une pneumatose kystique grêlique associée à une ascite. Cette association demeure être inhabituelle

Acrocyanosis and digital necrosis are associated with poor prognosis in COVID-19

Acrocyanosis and digital necrosis, which caused by microangiopathic and immunothrombosis phenomenon, may accompanied by microvascular involvement of other organs. Therefore, this finding can play a prognostic role in covid-19 outcome. © 2020 The Authors. Clinical Case Reports published by John Wiley & Sons Lt

The nature and rationale of the robotic curriculum in elementary school

Background and Objective:As widespread changes have occurred in the community, the use of technology has also expanded. To effectively prepare for and cope with evolving of this century we need to design and develop special curricula. Educational robotics is an advanced technology that requires special attention. In the past few decades, robotics has attracted the attention of researchers and teachers as a valuable tool in developing cognitive / social skills of students and in supporting the learning of subjects in science, mathematics, language, and technology. There are several important reasons why young students should be exposed to robotics. As our world becomes more and more technologically advanced, students need to early experience so that to feel comfortable with and be aware of technology. In addition, the inclusion of robotics in the school curriculum will help prepare students to enter the job market with technological literacy. Robotics is an attractive approach to technology training due to its interdisciplinary nature, which requires expertise in a wide range of fields from mathematics to aesthetics. This can attract the interest and engagement of students who have not been successful in traditional subject matters. The purpose of this paper is to explain the philosophical orientation and educational robotics foundations at the primary school level so that policymakers, engineers and curriculum developers can formulate curriculum models for implementation. Methods: In this research, educational robotics was analyzed and synthesized using the synthesis research method. Valid documents and research from the last four decades have been selected and categorized using a criterion-based purposive sampling technique. Findings: Synthesis findings indicate that robotics in schools work in two ways as an independent subject and as an educational enabler serving other topics. Logical justification of it is based on constructivist, including epistemological (personal and multidisciplinary), psychological (attention to motivation, creativity and etc.) and sociological foundations (interaction, predictability and etc.). Conclusion: In order to design a curriculum, a planner must first pay attention to the nature of the subject or knowledge and then proceed to develop a plan based on the orientations of the curriculum. Because the subject is robotics training in elementary school, the planner must pay attention to its nature first. The findings of this synthesis showed that robotics can serve in schools at all levels as an independent subject or as an educational enabler in the service of other subjects. Therefore, it is necessary to differentiate between the concepts of robotic training and training robotics. In robotics training, the subject is robot training; but in educational robotics, the robot is considered as a method, tool or technique that is used to teach other subjects.   ===================================================================================== COPYRIGHTS  ©2019 The author(s). This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, as long as the original authors and source are cited. No permission is required from the authors or the publishers.  ====================================================================================