19 research outputs found
Αντιστροφή φύλου του διακοσμητικού ψαριού μονομάχος (Betta splendens, Regan 1910)
<p>C2 phage bound to amyloid aggregates in 50 µm-thick brain tissue sections of temporal cortex from AD patients (A–D) and from cognitively normally aging (NA) elderly subjects (E). Each panel shows, from the left: the reactivity of phage (green); the reactivity of BAM 90.1, an anti-Aβ1–42 monoclonal antibody (red); the merge between red and green channels. A, C and E: scale bar represents 150 µm; B and D: scale bar represents 10 µm.</p
Variable epitope library-based COVID-19 vaccine for current and future related epidemics
The outbreak of yet another epidemic (now COVID-19 pandemic) caused by SARS-CoV-2, has reminded the scientific community and, more importantly, the world that in spite of all the financial and human resource investment in vaccine development against major diseases, the success rate has not been satisfactory. Accordingly, we must take extraordinary measures to try and contain the spread of this disease and find efficacious treatment options; the generation of prophylactic/therapeutic vaccines may resolve these objectives. </p
Data concerning secondary structure and alpha-glucans-binding capacity of the LaCBM26
Carbohydrate-binding modules (CBMs) are auxiliary domains into glycoside-hydrolases that allow the interaction between the insoluble substrate and the solubilized enzyme, through hydrophobic, CH-π interactions and hydrogen bonds. Here, we present the data article related to the interaction of one LaCBM26 and some mutated proteins with soluble α-glucans determined by enzyme-linked carbohydrate-binding assay, isothermal titration calorimetry (ITC), and affinity gel electrophoresis (AGE). The data of the behavior of proteins in presence and absence of substrate analyzed by circular dichroism CD and thermofluor are also presented. These results are complementary to the research article “The role of conserved non-aromatic residues in the Lactobacillus amylovorus α-amylase CBM26-starch interaction” (Armenta et al., 2019). Keywords: Carbohydrate-binding module, Starch, α-Glucans recognitio
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Amyloid-beta Peptide Binds to Cytochrome C Oxidase Subunit 1
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Amyloid-beta Peptide Binds to Cytochrome C Oxidase Subunit 1
Anti-11[E]-pyroglutamate-modified amyloid β antibodies cross-react with other pathological Aβ species: Relevance for immunotherapy
Western blot analysis of recombinant C2 phage expressing the fragment of CcOX1.
<p>10<sup>11</sup> phage particles diluted in loading buffer were resolved on 4–12% NuPAGE Bis-Tris gel (Invitrogen) at 200 V for 45 min at room temperature and immunoblotted for detection with anti-pIII antibody. Wild-type M13 phage was used as a control. Migration of the molecular mass standards as well as pIII and pIII–CcOX1 fusion protein are indicated by arrowheads.</p