GALNT2 mRNA levels are associated with serum triglycerides in humans

Atherogenic dyslipidemia, characterized by high triglycerides (TG) and low high density lipoprotein (HDL)-cholesterol levels, is a feature of patients with insulin resistance, obesity, and type 2 diabetes (T2D) [1] and plays a major role in shaping the risk of cardiovascular disease. Both TG and HDL-cholesterol serum concentrations are under the control of both environmental factors and up to 95 genetic loci, unraveled by a very large genome-wide association study (GWAs) in approximately 100,000 individuals [2]. Among these loci is GALNT2, which encode for ppGal-NAc-T2, involved in O-linked glycosylation. Similarly, studies in rodents have shown that liver GALNT2 expression modulates HDL-cholesterol concentrations [2]. Based on such studies, it is conceivable that GALNT2 expression changes play a role on TG and/or HDL-cholesterol levels. To gain further insights about this hypothesis, GALNT2 expression was measured in peripheral white blood cells (PWBC), from 224 individuals with a wide range of TG and HDL-cholesterol levels, as well as other metabolic parameters and clinical conditions

Evaluating a continuing professional development course on cognitive functions for Music Therapists working in care homes

Scarce research to date has addressed the aspect of Continuing Professional Development (CPD) as a key requirement for qualified music therapists. The present study investigated the benefits of a CPD course based on cognitive neuropsychology, which aimed to develop music therapists’ knowledge and skills in care home settings. The course included 32h of activities spread across 3 months and was attended by 31 music therapists. This course was evaluated using a mixed-methods approach including a semi-structured interview and a quantitative questionnaire. The results revealed that the CPD course brought different benefits meeting the needs of the therapists working in the care homes, which, included: i) improved general knowledge of music cognition, ii) broadened thinking about music therapy practice and clients’ abilities, and iii) an additional clinical and theoretical framework. These results are consistent with previous literature, highlighting the importance of providing advanced training for music therapists. Crucially, the findings highlighted the need for different strategies, techniques and pedagogical approaches in CPD courses, in function of the work setting, to improve attendees’ clinical skills. In addition, the study outlines how a CPD course may be tailored to enhance specific skills and transfer of learning in line with workplace demands

DETERMINATION OF IRRADIATION MARKERS IN FOODS

Today\u2019s food industry is faced with several important challenges, including food product deterioration and the constant increase of diseases related to the presence of pathogenic microorganisms in food products. Thus, adequate and effective food preservation strategies are even more important. Food irradiation is a technological process that can improve the microbiological quality of foodstuffs and extend the period in which it can be safely consumed. The radiation treatment, carried out under conditions of Good Manufacturing Practice, is considered as an effective, widely applicable food processing method judged to be safe on extensive available evidence. This technology can reduce the risk of food poisoning, control food spoilage and extend the shelf-life of foods without detriment to health and with minimal effect on nutritional or sensory quality. Due to its numerous positive effects, including those of a commercial nature, food irradiation has assumed a highly important role in the field of food preservation, and increasingly large numbers of foodstuffs are subjected to this treatment each year. For some time now, countries equipped with adequate food irradiation facilities have used this technology at well defined doses for the preservation of various foodstuffs. Because of the divergent opinions expressed by many consumers' organizations, the European Union has issued two directives (1999/2/EC and 1999/3/EC), which have been implemented in Italy by Legislative Decree No. 94 of 30 January 2001. Those directives aims at harmonizing the rules concerning the treatment and trade of irradiated foods in EU countries. With the open market, each country is obliged to accommodate the presence in its internal market of irradiated food commodities treated in other EU states or in extra-European countries. To further safeguard the consumer, the EU legislation provides for official annual checks at the product marketing stage, with the purpose of identifying improperly labeled or unauthorized products. Thus far, only limited food categories has been studied and subjected to interlaboratory validation by analytical detection methods for irradiated food identification. To meet the specific requirements of the laws and to increase acceptance of this type of food preservation technology, we have extended the field of application of both screening (PSL) and confirmatory (ESR, TL) physical methods to check compliance with labeling of irradiated foodstuffs. Therefore for consumer protection and information, following the invitation from the European Commission to improve and develop more reliable analytical standards, research work was focused on new applications of these physical methods. Relevant contributions have been made to the extension of the current field of application, with the development of promising analytical procedure able to estimate the actual dose administered to treated foods. The first goal was achieved investigating, even at low doses (0.1 kGy), the luminescence yield of oysters, considered a great delicacy in many parts of the world, and validating its identification with two physical techniques: PSL as screening method and TL as a confirmatory one. Besides oysters other seafood, including bivalve mollusks, i.e. brown Venus shells, clams, and mussels, all of which are widely consumed and likely to be treated with irradiation were studied with Electron Spin Resonance (ESR) spectroscopy. It is well known that irradiation by ionising radiation leads to the formation of many radical species which, if stable, could be detected in calcified tissue such as mollusks' shell. Identification of four irradiated species of bivalve mollusks, i.e. brown Venus shells (Callista chione), clams (Tapes semidecussatus), mussels (Mytilus galloprovincialis) and oysters (Ostrea edulis) was performed. ESR could definitely identify irradiated seashells due to the presence of long-lived free radicals, primarily CO2-, CO33-, SO2- and SO3- radical anions. The presence of other organic free radicals, believed to originate from conchiolin, a scleroprotein present in the shells, was also ascertained. The use of one of these radicals as a marker for irradiation of brown Venus shells and clams can be envisaged. In addition to detection procedures a reliable dosimetric protocol for the reconstruction of the administered dose in irradiated oysters was proposed. Finally the results of a study on official checks by an accredited laboratory aimed at both evaluating the performances of detection methods and the presence of irradiated food on the Italian market, are discussed. Non-compliances found are due to both incorrect labelling and irradiation in non approved facilities in extra European countries. In summary, two physical methods, electron spin resonance (ESR) spectroscopy and thermoluminescence (TL) were studied most extensively and applied on a wide range of foods with successful results, whereas limitations of current standards were also assessed. The development and application of analytical methods for correct identification of irradiated samples from non-irradiated samples, along with protocols for dose evaluation, have become important for several purposes: upholding regulatory controls, checking compliance against labeling requirements, facilitating international trade, and reinforcing consumer confidence. Therefore the research on new detection methods represents a key area and more studies in this field should be encouraged

Development and validation of the Music Cognitive Test: a music-based cognitive screening test

Specific tools to measure the cognitive benefits of music therapy and music-rehabilitation training available to music therapists are few and empirically weak: they are mostly psychometrically unrefined or based on unclear tasks and scoring protocols; they do not take into consideration distinct cognitive functions or are based on exclusively observational protocols. To overcome these limitations, we developed a 15-min cognitive screening tool suitable for music therapists, MCT (Music Cognitive Test), which assesses cognitive abilities stimulated by music-making activities (e.g., attentional, verbal and executive functions, short- and long-term memory) by including music-based items. MCT was validated with 335 participants (aged 18-100y.o.) presenting a range of cognitive levels, from healthy cognition to severe impairment. MCT correlated strongly and positively with well-known tests: MMSE (Mini-Mental State Examination), MoCA (Montreal Cognitive Assessment) and SMMSE (Severe Mini-Mental State Examination). MCT also displayed excellent sensitivity in identifying impaired individuals according to both MMSE and MoCA diagnostic criteria (99.4% and 93.0%, respectively), and excellent specificity in identifying healthy participants based on MMSE (93.5%) and MoCA (97.8%) criteria. Overall, results highlight the reliability of this novel brief music-focused cognitive screening test, to enable music therapists to independently and consistently monitor the effectiveness of their intervention on cognitive functions

Colour variation of the Maltese wall lizards (Podarcis filfolensis) at population and individual levels in the Linosa island

AbstractThe research on animal colouration has always been of great interest for biologists but since the last decades it has grown exponentially thanks to multidisciplinary approaches. Animals have found several ways to deal with the camouflage/communication trade-off in colouration, leading to the evolution of alternative patterns of variation of colourations at different levels including signal partitioning and spatial resolution of colouration. In this paper we analyse the variability of dorsal and ventral colouration in males and females of Maltese wall lizards in three populations on Linosa. We collected high-resolution digital images of dorsal, ventral and throat colouration from 61 lizards (32 males and 29 females). We showed that the colouration differs among sexes and body regions within the same individual. Colourations are also variable among individuals within population, as well as among different populations across the Island. Finally, we detected a lizard's colouration shifts with increasing body size. Those result supports the hypothesis that colouration in this species evolved under the competing pressures of natural and sexual selection to promote signals that are visible to conspecifics while being less perceptible to avian predators. Graphic abstrac

GALNT2 as a novel modulator of adipogenesis and adipocyte insulin signaling

Background/objectives: A better understanding of adipose tissue biology is crucial to tackle insulin resistance and eventually coronary heart disease and diabetes, leading causes of morbidity and mortality worldwide. GALNT2, a GalNAc-transferase, positively modulates insulin signaling in human liver cells by down-regulating ENPP1, an insulin signaling inhibitor. GALNT2 expression is increased in adipose tissue of obese as compared to that of non-obese individuals. Whether this association is secondary to a GALNT2-insulin sensitizing effect exerted also in adipocytes is unknown. We then investigated in mouse 3T3-L1 adipocytes the GALNT2 effect on adipogenesis, insulin signaling and expression levels of both Enpp1 and 72 adipogenesis-related genes. Methods: Stable over-expressing GALNT2 and GFP preadipocytes (T 0 ) were generated. Adipogenesis was induced with (R+) or without (R−) rosiglitazone and investigated after 15 days (T 15 ). Lipid accumulation (by Oil Red-O staining) and intracellular triglycerides (by fluorimetric assay) were measured. Lipid droplets (LD) measures were analyzed at confocal microscope. Gene expression was assessed by RT-PCR and insulin-induced insulin receptor (IR), IRS1, JNK and AKT phosphorylation by Western blot. Results: Lipid accumulation, triglycerides and LD measures progressively increased from T 0 to T 15 R- and furthermore to T 15 R+. Such increases were significantly higher in GALNT2 than in GFP cells so that, as compared to T 15 R+GFP, T 15 R- GALNT2 cells showed similar (intracellular lipid and triglycerides accumulation) or even higher (LD measures, p < 0.01) values. In GALNT2 preadipocytes, insulin-induced IR, IRS1 and AKT activation was higher than that in GFP cells. GALNT2 effect was totally abolished during adipocyte maturation and completely reversed at late stage maturation. Such GALNT2 effect trajectory was paralleled by coordinated changes in the expression of Enpp1 and adipocyte-maturation key genes. Conclusions: GALNT2 is a novel modulator of adipogenesis and related cellular phenotypes, thus becoming a potential target for tackling the obesity epidemics and its devastating sequelae

A tribute to Hubert Saint Girons: niche separation between Vipera aspis and V. berus on the basis of distribution models

Interspecific competition in contact areas is a major topic in ecological studies. A number of studies were carried out on European vipers, focusing on contact areas between two or three species characterized as appropriate by ecological needs more or less similar. The aim of this study is to extend the analysis of this topic to the case of Vipera aspis and V. berus in an alpine area of northern Italy, by comparing suitability models to verify which ecological factors affect their occurrence and to assess a possible niche separation. Potential distribution was modelled using the maximum entropy method, using six non-correlated ecogeographical variables as predictors. The models fitted well for both species (mean AUC = 0.926; 87.4% of testing data correctly classified). The most informative variables were: habitat, altitude and solar radiation for the asp viper; altitude and habitat for the adder. Deciduous woods, meadows and urban areas had a positive effect on V. aspis distribution as wetlands, meadows and rocks vegetation did on V. berus. However, the variable best separating the species was the elevation, the adder occurring more frequently at higher altitude than the asp viper. Our data showed that the two vipers were mutually exclusive, as already observed by Saint Girons in 1975. Vipera aspis is more thermophilic and lives at low altitude, while Vipera berus lives under cool and humid areas typical of alpine pastures. A similar pattern were found in the contact areas between European vipers belonging to the V. aspis and Pelias group respectively

The impact of temporal synchronisation imprecision on TRF analyses

Human sensory perception requires our brains to extract, encode, and process multiple properties of the sensory input. In the context of continuous sensory signals, such as speech and music, the measured electrical neural activity synchronises to properties such as the acoustic envelope, a phenomenon referred to as neural tracking. The ability of measuring neural tracking with non-invasive neurophysiology constitutes an exciting new opportunity for applied research. For example, it enables the objective assessment of cognitive functions in challenging cohorts and environments by using pleasant, everyday tasks, such as watching videos. However, neural tracking has been mostly studied in controlled, laboratory environments guaranteeing precise synchronisation between the neural signal and the corresponding labels (e.g., speech envelope). There exist various challenges that could impact such a temporal precision in, for instance, out-of-lab scenarios, such as technology (e.g., wireless data acquisition), mobility requirements (e.g., clinical scenarios), and the task (e.g., imagery). Aiming to address this type of challenge, we focus on the predominant scenario of continuous sensory experiments involving listening to speech and music. First a temporal response function analysis is presented on two different datasets to assess the impact of trigger imprecision. Second, a proof-of-concept re-alignment methodology is proposed to determine potential issues with the temporal synchronisation. Finally, a use-case study is presented that demonstrates neural tracking measurements in a challenging scenario involving older individuals with neurocognitive decline in care homes. Significance Statement Human cognitive functions can be studied by measuring neural tracking with non-invasive neurophysiology as participants perform pleasant, everyday tasks, such as listening to music. However, while recent work has encouraged the use of this approach in applied research, it remains unclear how robust neural tracking measurements can be when considering the methodological constraints of applied scenarios. This study determines the impact of a key factor for the measurement of neural tracking: the temporal precision of the neural recording. The results provide clear guidelines for future research, indicating what level of imprecision can be tolerated for measuring neural tracking with speech and music listening tasks in both laboratory and applied settings. Furthermore, the study provides a strategy to assess the impact of imprecision in the synchronisation of the neural recording, thus developing new tools for applied neuroscience

Chromatographic determination of 12 dyes in meat products by HPLC-UV-DIODE array detection

The use of food dyes in meat is regulated by the current European and non-European legislation, due to several food safety concerns. A reliable method for the quali-quantitative determination of 12 food dyes (Amaranth, Ponceau 4R, Carmine, Ponceau SX, Ponceau 3R, Allura Red AC, Carmoisine, Erythrosine, Sudan I, Sudan II, Sudan III and Sudan IV) in meat products, by high performance liquid chromatography coupled to UV diode array detection is presented. The extraction was accomplished by using acetonitrile, methanol, water, and ammonia, 50:40:9:1 (v/v/v/v) as the solvent and ultrasonic bath. The chromatographic separation was obtained with a C18 RP column eluted by a gradient of acetate buffer/acetonitrile. Good analytical performances characterized this method (Table 1), in terms of selectivity, sensitivity, accuracy and ruggedness. Both method precision (CV% range: 6%-15%) and recovery percentages (range: 86%-105%) resulted in compliance with Decision 2002/657/EC, and the expanded measurement uncertainties, estimated by a bottom-up approach, were in the range 6%-20%. All these results demonstrated that the procedure can be applied successfully for confirmation analyses of commercial meat products. •12 food dyes were determined in meat by new HPLC/UV-DAD method.•The analytical method was fully validated for accurate confirmation analyses.•Method accuracy, sensitivity, selectivity and ruggedness resulted satisfactory