Proteolysis as a function of distance from surface to centre in a smear-ripened Irish farmhouse cheese

This study focused on proteolysis in an Irish farmhouse smear-ripened cheese by serial slicing (0.41 mm/slice) the first 2 cm from surface towards the centre of the cheese. Ureapolyacrylamide gel electrophoretograms confirmed higher proteolysis in the outer layers than at the centre. Free amino acid (FAA) analysis confirmed decrease in proteolytic activity from surface to centre. Peptides produced at depths 0.41 mm and 20.5 mm were 720 and 427 from αs1-casein; 691 and 337 from αs2-casein; 807 and 453 from β-casein; 180 and 109 from κ-casein. The study confirms higher proteolytic activity at surface due to action of enzymes of the smear microbiota, than at the centre of cheese and identified the agents responsible for production of many peptides

Trace Element Abundances in an Unusual Hibonite-Perovskite Refractory Inclusion from Allende

Calcium-aluminum-rich refractory inclusions (CAIs) are thought to be the first-formed solids in the Solar protoplanetary disk and can provide information about the earliest Solar System processes (e.g., [1]). A hibonite-perovskitebearing CAI from the Allende CV3 chondrite (SHAL, [2]) contains a single of 500 micrometers hibonite grain and coarse-grained perovskite. The mineralogy and oxygen isotopic composition of this CAI shows similarities with FUN inclusions, especially HAL [2]. Here we present trace element abundances in SHAL

A CLINICAL STUDY OF MARICHA CHURNA AND PIPPALI MOOL IN THE MANAGEMENT OF STANYAKSHAYA

Sthanya is the Updhatu of Rasa dhatu. Ayurveda explain the importance of Sthanya through its main function Pushti and Jeevan. Rasa DHATU is said to Addidhatu i.e. Prathamdhatu. If Rasa dhatu formation is disturbed, its Updhatusthanya is also get disturbed. In Stanyakshaya there is Kshaya due to Dhatukshaya and Dushti. In present study, “effect of Maricha pippalimool churna on Sthanyakshaya’’ has been designed by taking reference from Kashyapsamhita Ksheeroudpadya. The purpose is to analyse and evaluate the complete concept and its etiopathogenesis of Sthanyakshaya and treatment with Ikshumulkashya as whole in light of Ayurvedic and modern concept. The aim is to study causes of Stanyakshaya which occurs with different reasons prescribed in Ayurveda like Krodha, Shoka etc., and according to modern science stress, anaemia etc., and to determine the effectiveness of Maricha Pippalimool churna on galactagogue, improve the detail study about Stanyakshya from Ayurvedic and modern text. The drugs used for this clinical study are Maricha Churna, Pippalimool Churna. 60 patients clinically diagnosed as Stanyakshya patients were selected. Statistical analysis of the parameters: Socio-demographic profile, ejection breast milk, duration of breast feeding, according to Stanapurnatva, according to frequency of feeding, weight of infant, frequency of urine, sleep of infant was done. The drug “Maricha pippalimool churna” was given for 30 days. Significant increase in parameter had been observed during the study. There was increase in Stanya after application of drug “Maricha pippalimool churna” given in Stanyakshaya. This treatment does not show any toxicity and no side effects were seen during the follow up. This remedy is economical and easy to use, store and carry

Proteolysis in Irish farmhouse Camembert cheese during ripening

Proteolysis in an Irish farmhouse Camembert cheese was studied during 10 weeks of ripening. Urea‐polyacrylamide gel electrophoresis of pH 4.6‐insoluble fractions of cheese showed the degradation of caseins, initially due to the action of chymosin and plasmin and later due to Penicillium camemberti proteinases. Proteolytic specificities of Penicillium camemberti proteinases on the caseins in milk hydrolysates were determined and 64, 6, 28, and 2 cleavage sites were identified in αs1‐, αs2‐, β‐, and κ‐casein, respectively. Proteolysis in cheese was studied and peptides produced were determined and compared to the cleavage specificities of Penicillium camemberti proteinases. Regions most susceptible to proteolysis were 1–40, 79–114, and 168–199 in αs1‐casein; 42–79 and 97–116 in αs2‐casein; 40–57, 101–125, 143–189, and 165–209 in β‐casein; and 31–81 and 124–137 in κ‐casein. The present study describes in detail the proteolytic action of proteinases from Penicillium camemberti in Camembert cheese during ripening. Practical applications: Camembert cheese is a major international cheese variety, made in many countries around the world. The ripening of the cheese involves many biochemical changes and this study provides new information on peptides produced during ripening. Penicillium camemberti is an important mold used in the production of this type of cheese and detailed information is provided on the action of its enzymes on the caseins. Data reported in this study furthers the understanding of the ripening of Camembert cheese

A tracking algorithm for the stable spin polarization field in storage rings using stroboscopic averaging

Polarized protons have never been accelerated to more than about $25$GeV. To achieve polarized proton beams in RHIC (250GeV), HERA (820GeV), and the TEVATRON (900GeV), ideas and techniques new to accelerator physics are needed. In this publication we will stress an important aspect of very high energy polarized proton beams, namely the fact that the equilibrium polarization direction can vary substantially across the beam in the interaction region of a high energy experiment when no countermeasure is taken. Such a divergence of the polarization direction would not only diminish the average polarization available to the particle physics experiment, but it would also make the polarization involved in each collision analyzed in a detector strongly dependent on the phase space position of the interacting particle. In order to analyze and compensate this effect, methods for computing the equilibrium polarization direction are needed. In this paper we introduce the method of stroboscopic averaging, which computes this direction in a very efficient way. Since only tracking data is needed, our method can be implemented easily in existing spin tracking programs. Several examples demonstrate the importance of the spin divergence and the applicability of stroboscopic averaging.Comment: 39 page

Transcriptome sequencing of the Microarray Quality Control (MAQC) RNA reference samples using next generation sequencing

<p>Abstract</p> <p>Background</p> <p>Transcriptome sequencing using next-generation sequencing platforms will soon be competing with DNA microarray technologies for global gene expression analysis. As a preliminary evaluation of these promising technologies, we performed deep sequencing of cDNA synthesized from the Microarray Quality Control (MAQC) reference RNA samples using Roche's 454 Genome Sequencer FLX.</p> <p>Results</p> <p>We generated more that 3.6 million sequence reads of average length 250 bp for the MAQC A and B samples and introduced a data analysis pipeline for translating cDNA read counts into gene expression levels. Using BLAST, 90% of the reads mapped to the human genome and 64% of the reads mapped to the RefSeq database of well annotated genes with e-values ≤ 10^-20. We measured gene expression levels in the A and B samples by counting the numbers of reads that mapped to individual RefSeq genes in multiple sequencing runs to evaluate the MAQC quality metrics for reproducibility, sensitivity, specificity, and accuracy and compared the results with DNA microarrays and Quantitative RT-PCR (QRTPCR) from the MAQC studies. In addition, 88% of the reads were successfully aligned directly to the human genome using the AceView alignment programs with an average 90% sequence similarity to identify 137,899 unique exon junctions, including 22,193 new exon junctions not yet contained in the RefSeq database.</p> <p>Conclusion</p> <p>Using the MAQC metrics for evaluating the performance of gene expression platforms, the ExpressSeq results for gene expression levels showed excellent reproducibility, sensitivity, and specificity that improved systematically with increasing shotgun sequencing depth, and quantitative accuracy that was comparable to DNA microarrays and QRTPCR. In addition, a careful mapping of the reads to the genome using the AceView alignment programs shed new light on the complexity of the human transcriptome including the discovery of thousands of new splice variants.</p

Hetero-Diels–Alder Cycloaddition with RAFT Polymers as Bioconjugation Platform

We introduce the bioconjugation of polymers synthesized by RAFT polymerization, bearing no specific functional end group, by means of hetero‐Diels–Alder cycloaddition through their inherent terminal thiocarbonylthio moiety with a diene‐modified model protein. Quantitative conjugation occurs over the course of a few hours, at ambient temperature and neutral pH, and in the absence of any catalyst. Our technology platform affords thermoresponsive bioconjugates, whose aggregation is solely controlled by the polymer chains

Hetero-Diels-Alder-Cycloaddition mit RAFT-Polymeren als Biokonjugationsplattform

Wir stellen die Biokonjugation von Polymeren vor, die durch RAFT‐Polymerisation mittels Hetero‐Diels‐Alder‐Cycloaddition durch ihren inhärenten terminalen Thiocarbonylthiorest mit einem dienmodifizierten Modellprotein synthetisiert wurden und keine spezifische funktionelle Endgruppe tragen. Die quantitative Konjugation erfolgt im Verlauf einiger Stunden bei Raumtemperatur und nahezu neutralem pH‐Wert und in Abwesenheit jeglichen Katalysators. Unsere Technologieplattform liefert thermoresponsive Biokonjugate, deren Aggregation allein durch die Polymerketten gesteuert wird

An Integrated Circuit for Signal Processing of the AMS RICH Photmultipliers Tubes

An analog integrated circuit has been designed, in a BiCMOS 0.8 micron technology, for the feasability study of the signal processing of the AMS RICH photomultiplier tubes. This low power, three channel gated integrator includes its own gate and no external analog delay is requiered. It processes PMT pulses over a dynamic range of more than 100. A logic output that indicates whether the analog charge has to be considered is provided. This gated integrator is used with a compact DSP based acquisition system in a 132 channels RICH prototype. The charge calibration of each channel is carried out using a LED. The pedestal measurement is performed on activation of a dedicated input. The noise contribution study of the input RC network and amplifiers is presented.Comment: IEEE symp. on Nucl. Sci. and Med. Imaging, Toront