Uticaj postoperativne terapije laserom male snage na oseointegraciju samourezujućih implantata u bočnoj regiji gornje vilice - šestonedeljna split-mouth klinička studija

Background/Aim. Low-level laser therapy (LLLT) has been proven to stimulate bone repair, affecting cellular proliferation, differentiation and adhesion, and has shown a potential to reduce the healing time following implant placement. The aim of this clinical study was to investigate the influence of postoperative LLLT osseointegration and early success of self-tapping implants placed into low-density bone. Methods. Following the split-mouth design, self-tapping implants (n = 44) were inserted in the posterior maxilla of 12 patients. One jaw side randomly received LLLT (test group), while the other side was placebo (control group). For LLLT, a 637 nm gallium-aluminum-arsenide (GaAlAs) laser (Medicolaser 637, Technoline, Belgrade, Serbia) with an output power of 40 mW and continuous wave was used. Low-level laser treatment was performed immediately after the surgery and then repeated every day in the following 7 days. The total irradiation dose per treatment was 6.26 J/cm² per implant. The study outcomes were: implant stability, alkaline-phosphatase (ALP) activity and early implant success rate. The follow-up took 6 weeks. Results. Irradiated implants achieved a higher stability compared with controls during the entire follow-up and the difference reached significance in the 5th postoperative week (paired t-test, p = 0.030). The difference in ALP activ- ity between the groups was insignificant in any observation point (paired t-test, p > 0.05). The early implant success rate was 100%, regardless of LLLT usage. Conclusion. LLLT applied daily during the first postoperative week expressed no significant influence on the osseointegration of self- tapping implants placed into low density bone of the posterior maxilla. Placement of self-tapping macro-designed implants into low density bone could be a predictable therapeutic procedure with a high early success rate regardless of LLLT usage.Uvod/Cilj. Terapija laserom male snage (TLMS) stimuliše reparatorne sposobnosti kosti utičući na ćelijsku proliferaciju, diferencijaciju i adheziju, i ima potencijal da skrati vreme zarastanja kosti nakon ugradnje implantata. Cilj ove kliničke studije bio je da se ispita uticaj postoperativne primene TLMS na oseointegraciju i rani uspeh ugradnje samourezujućih implantata u kost male gustine. Metode. Prateći split- mouth dizajn, samourezujući implantati (n = 44) ugrađeni su u posteriorne regije gornje vilice 12 pacijenata. Slučajnim izborom, jednoj od strana vilice je dodeljena TLMS (test grupa), dok je druga strana bila placebo (kontrolna grupa). Za TLMS korišćen je galijum-aluminijum-arsenid (GaAlAs) laser (Medicolaser 637, Technoline, Beograd, Srbija) talasne dužine 637 nm, snage 40 mW, neprekidnog režima rada. Tretman laserom male snage sprovodio se neposredno po ugradnji, a zatim svakodnevno, tokom narednih sedam dana. Ukupna zračna doza po tretmanu bila je 6,26 J/cm² po implantatu. Praćeni su stabilnost implantata, aktivnost alkalne fosfataze (ALP) i procenat rane uspešnosti implantatne terapije. Period praćenja bio je šest nedelja. Rezultati. Zračeni implantati imali su veću stabilnost u odnosu na kontrolne tokom celog perioda praćenja, a statistički značajno veća stabilnost bila je u petoj postoperativnoj nedelji (t-test za vezane uzorke, p = 0.030). Razlika u aktivnosti ALP između grupa nije bila statistički značajna ni u jednoj tački posmatranja (t-test za vezane uzorke, p > 0.05). Procenat rane uspešnosti terapije implantatima bio je 100%, bez obzira na primenjenu TLMS. Zaključak. Svakodnevna primena TLMS u prvoj postoperativnoj nedelji nije pokazala značajan uticaj na oseointegraciju samourezujućih implantata u kost male gustine bočne regije gornje vilice. Primena implantata samourezujućeg makrodizajna u kosti male gustine mogla bi predstavljati predvidljivu terapijsku proceduru sa visokim procentom rane uspešnosti, bez obzira na primenjenu TLMS

Interstitial brachytherapy with 192Ir in vulvar cancer

Vulvar cancer accounts for 5% of all female genital cancers and 1% of all malignancies in women. A female patient, 78 years old, with diagnosed squamous cell carcinoma of vulva stage II, was admitted to our Institution in January 2004. An exophytic tumor, size 6 x 3.5 cm which infiltrated subcutis in the area of the upper two-thirds of labia majora dexter was found during gynecological examination. Inguinal nodes were negative. Our choice was to perform interstitial brachytherapy. During the control checkup in September 2005 no tumor was observed and its previous location was transformed in fibrous tissue with shallow post-irradiative crater

Uticaj bakterijskog inokulanta na kvalitet silaže i senaže lucerke

Using of silage and haylage of forage legumes in ruminant nutrition and promotion of promoting proper forage conservation techniques should be an important strategy in livestock production in our country. Forage legumes are difficult to ensile, so it is necessary to apply the starter culture of selected strains of lactic acid bacteria that support the ensiling process and prevent bacterial butyric fermentation and thus contribute to the preservation and improvement of silage and haylage quality. In this paper, the influence of bacterial inoculant ‘Silko for alfalfa’ on the quality of silage and haylage of alfalfa in two separate trials is presented. The inoculant is a combination of homofermentative lactic bacteria Lactobacillus plantarum and Pediococcus spp. The first-cut alfalfa in the second year was used for silage and haylage. The silage was examined in mini-silos in the laboratory, and the haylage at the cattle farm where the plant material was cuts were collected in experimental silo bags. The treatments were control (untreated silage, i.e. haylage) and silage, i.e. haylage treated with inoculant ‘Silko for alfalfa’ (rate of 5 ml/t fresh material). The silages were analyzed after 90 days, and haylage after 40. The inoculant ‘Silko for alfalfa’ has been found to maintain the nutritive value of silage and haylage and to improve their chemical, energy and fermentation parameters relative to the control. Since ‘Silko for alfalfa’ positively affects the correct lactic acid fermentation of silage and haylage and contributes to a lesser loss of nutritional value and energy it is expected that it can promote a high level of productivity of ruminants, and thus contribute to the growth of profit in livestock production.Važna strategija u stočarstvu treba da bude uvođenje silaža i senaža krmnih leguminoza u ishranu preživara i promovisanje pravilnih tehnika siliranja i senažiranja. Krmne leguminoze se teško siliraju pa je neophodno primeniti starter kulture odabranih sojeva bakterija mlečne kiseline koje podržavaju proces siliranja i sprečavaju buternu fermentaciju i time doprinose očuvanju i unapređenju kvaliteta silaža. U radu je prikazan uticaj primene bakterijskog inokulnata ‘Silko za lucerku’ na kvalitet silaže i senaže u dva odvojena eksperimenta. Inokulant predstavlja kombinaciju homofermentativnih mlečnih bakterija Lactobacillus plantarum i Pediococcus spp. Za siliranje su korišćeni prvi otkosi lucerke u drugoj godini eksploatacije. Silaža je ispitivana u mini-silosima u laboratoriji, a senaža u silo vrećama na govedarskoj farmi. Tretmani su bili kontrola (netretirana silaža, odnosno senaža) i silaža, odnosno senaža tretirana sa inokulantom ‘Silko za lucerku’ (doza 5 ml/t krme). Silaže pripremljene u eksperimentalnim uslovima su analizirane nakon 90 dana, a senaže dobijene u proizvodnim uslovima nakon 40 dana. Ustanovljeno je da korišćeni inokulant čuva nutritivnu vrednost silaže i senaže i da poboljšava njihove hemijske, energetske i fermentacione parametre u odnosu na kontrolu. S obzirom da ispitivani inokulant pozitivno utiče na pravilnu mlečno-kiselinsku fermentacju silaže i senaže lucerke i doprinosi manjem gubitku hranljive vrednosti i energije za očekivati je da može promovisati visok nivo produktivnosti preživara, a samim tim i doprineti rastu profita u stočarstvu

Stanje oralnog zdravlja dece sa posebnim potrebama

Background/Aim. Due to their primary medical condition, children with special needs often display lower levels of oral hygiene, larger prevalence of caries and other oral diseases. The aim of this study was to estimate the prevalence of dental caries, oral cleanliness and presence of malocclusion in children with disabilities, as well as to evaluate eruption time of the permanent molars. Methods. Case-control study was carried out on a group of 107 children with disabilities at the Faculty of Dental Medicine, University of Belgrade, Serbia. The control group comprised of 104 healthy school children. Results. Children with disabilities had statistically higher mean [decayed missing and filled teeth - dmft for primary DMF for permanent dentition (dmft DMFT)] values in both dentitions than children from the control group (p lt 0.05). Oral cleanliness level was much lower in children with disabilities. A significantly higher percentage of Class II malocclusions and a higher tendency to have a delayed time of eruption of permanent molars were observed in the test group in permanent dentition. Conclusion. Considering poor oral health status and higher tendency for development of malloclusions and delayed eruption, it is necessary to develop preventive dental programmes for children with special needs, as well as improve public awareness about these issues.Uvod/Cilj. Deca sa posebnim potrebama često zbog svoje primarne bolesti, imaju niži nivo oralne higijene i veću prevalencu karijesa i drugih oralnih oboljenja. Cilj rada bio je da se proceni prevalenca karijesa, nivoa oralne higijene i prisustvo malokluzija kod dece sa posebnim potrebama, kao i vreme erupcije stalnih molara. Metode. Ispitivanjem oralnog zdravlja obuhvaćeno je 107 dece sa posebnim potrebama na Klinici za dečju i preventivnu stomatologiju Stomatološkog fakulteta u Beogradu. Kontrolnu grupu je činilo 104 zdrave školske dece. Rezultati. Deca sa posebnim potrebama imala su statistički značajno viši nivo indeksa karijesnih, ekstrahovanih i plombiranih zuba (KEP) u obe denticije, u odnosu na kontrolnu grupu (p lt 0.05). Nivo oralne higijene bio je lošiji kod dece sa posebnim potrebama. Takođe, primećeno je statistički značajno povećanje malokluzija klase II, kao i kasnije vreme erupcije stalnih molara kod dece sa posebnim potrebama u odnosu na kontrolnu grupu. Zaključak. Zbog lošijeg stanja oralnog zdravlja i povećane verovatnoće razvoja malokluzija i odloženog nicanja zuba, neophodno je formirati preventivne stomatološke programe za decu sa posebnim potrebama, kao i poboljšati informisanost javnosti o ovom problemu

Chemical Composition, Total Phenols and Flavonoids Contents and Antioxidant Activity as Nutritive Potential of Roasted Hazelnut Skins (Corylus avellana L.)

The present study evaluates natural composition of Serbian roasted hazelnut skins (HS) with potential role in application as functional nutrient of various food products. Total phenols (TPC) and flavonoids contents (TFC) in HS extracts obtained with dierent ethanol concentrations (10%—I, 50%—II and 96%—III) and their antioxidant activities were investigated. The highest total phenols content (706.0 9.7 mgGAE/gextract) was observed in 96% ethanol HS extract. Ethanol HS extracts showed very high antioxidant activity with eective concentrations (EC50) ranged between 0.052 and 0.066 mg/mL. The phenol and flavonoid content of roasted HS extracts I–III was determined by HPLC-ESI-MS/MS analyses. Contents of lipids, proteins, carbohydrates, metals, and C, H, N, S elements in roasted HS were also determined. Relatively high C/N, C/P and C/N/P ratios, rich metal contents and fatty acids composition indicated that hazelnut skin might be a good candidate for use as either human or fungal functional nutrient. In addition, possible application of phenolic HS extracts as UV booster was studied by recording UV spectra (220–440 nm) of 10 mg/L of HS extracts I–III combined with 10 mg/L of chemical sunscreen agent benzophenone-3 and in vitro sun protection factor (SPF) was calculated

Uticaj različitih hemijskih sredstava i uslova skladištenja na mikrobiološki profil semena industrijske konoplje (Cannabis sativa L.)

This study aimed to test different chemical agents to obtain microbiologically safe industrial hemp seeds that could be used for further food processing (with the reduced total number of microorganisms, total number of moulds and yeasts, and total number of Enterobacteriaceae). In order to obtain seeds applicable for food consumption, optimal storage temperature conditions (room temperature, refrigerator, freezer), method of seed packaging (vacuum/without vacuum), and the application of various chemical treatments (ethanol, sodium hydrogen carbonate, sodium hypochlorite) were tested on the certified industrial hemp seeds, produced in two consecutive years. Optimal storage conditions differed for different microorganisms, and the most optimal storage was at room temperature, for seeds produced in 2018, in the treatment to reduce the total number of Enterobacteriaceae and the total number of microorganisms. When storing seeds from 2018 in order to reduce the number of yeasts and moulds, a slightly lower number was spotted when seeds were stored in a vacuum-sealed bag, at the refrigerator/freezer temperature. For hemp seeds produced in 2019, the most optimal storage conditions were at the refrigerator (for reduction of the total number of Enterobacteriaceae) and freezer temperature (for reduction of the total number of microorganisms). For the reduction of the total number of moulds and yeasts, optimal conditions were at room temperature. Ethanol (75%, v/v) was the most effective disinfectant among the tested chemicals regardless of the initial number of microorganisms, with log reduction of 3.2 (for the total number of Enterobacteriaceae), 2.9 log (for the total number of microorganisms), and total reduction of the total number of yeasts and moulds after 10 minutes, for the seeds harvested in 2019, which were far more contaminated than the seeds harvested in 2018. Considering the price of the disinfection method with ethanol, sodium hypochlorite may be a better solution for the reduction of the number of microbiota on the seeds.Cilj ovog istraživanja bio je ispitivanje različitih hemijskih sredstava za dobijanje mikrobiološki bezbednog semena industrijske konoplje, koje bi se moglo koristiti za dalju preradu u hranu (sa smanjenim ukupnim brojem mikroorganizama, ukupnim brojem kvasaca i plesni, i ukupnim brojem enterobakterija). Da bi se dobilo seme primenljivo za ishranu, optimalni temperaturni uslovi skladištenja (sobna temperatura, frižider, zamrzivač), način pakovanja semena (vakuum/bez vakuuma) i primena različitih hemijskih tretmana (etanol, natrijum hidrogen karbonat, natrijum hipohlorit) su testirani na sertifikovanom semenu industrijske konoplje, proizvedenom u dve uzastopne godine. Optimalni uslovi skladištenja su se razlikovali za različite mikroorganizme, a najoptimalnije skladištenje je bilo na sobnoj temperaturi, za seme iz 2018. godine, a u cilju smanjenja broja enterobakterija i ukupnog broja mikroorganizama. Pri skladištenju semena iz 2018. godine u cilju smanjenja broja plesni i kvasaca primećen je nešto manji broj datih mikroorganizama kada je seme čuvano u vakuumski zatvorenoj vrećici, na temperaturi frižidera/zamrzivača. Za seme konoplje iz 2019. godine najoptimalniji uslovi skladištenja su bili na temperaturi frižidera (za redukciju broja enterobakterija) i u zamrzivaču (za redukciju ukupnog broja mikroorganizama). Za smanjenje ukupnog broja plesni i kvasaca, optimalni uslovi su bili na sobnoj temperaturi. Etanol (75%, v/v) je bio najefikasnije sredstvo za dezinfekciju među ispitivanim hemijskim sredstvima, bez obzira na početni broj mikroorganizama, sa log smanjenjem od 3,2 (za ukupan broj enterobakterija), 2,9 log (za ukupan broj mikroorganizama) i ukupnim smanjenjem broja kvasaca i plesni nakon 10 minuta delovanja, za seme proizvedeno 2019. godine, koje je bilo mnogo više kontaminirano u odnosu na seme iz 2018. godine. Uzimajući u obzir cenu metoda dezinfekcije etanolom, natrijum hipohlorit može biti bolje rešenje za smanjenje broja mikrobiota na semenu

Intravitrealna primjena bevacizumaba u liječenju neovaskularnog glaukoma

The aim of the study was to assess short-term efficacy of intravitreal bevacizumab in a series of patients with neovascular glaucoma. Eleven patients with neovascular glaucoma and symptomatic elevation of intraocular pressure were treated with 1.25 mg/0.1 mL of bevacizumab. In three patients, intravitreal bevacizumab was administered preoperatively, one day before pars plana vitrectomy. Additional therapy was only performed if topical medication failed to result in satisfactory control of intraocular pressure. Patients were followed-up for a minimum of 8 weeks. In all study patients, intravitreal application of bevacizumab resulted in marked regression of iris neovascularization within the first three postoperative days. Appropriate control of intraocular pressure was achieved in seven patients, whereas four patients required additional therapy for intraocular pressure regulation. No side effects of intravitreal bevacizumab were recorded. Thus, intravitreal bevacizumab seems to be a potent adjunct in the management of neovascular glaucoma. Additional studies of bevacizumab long-term safety and efficacy are warranted.Cilj ove studije bio je procijeniti kratkotrajni učinak intravitrealne primjene bevacizumaba u skupini bolesnika s neovaskularnim glaukomom. U studiju je bilo uključeno jedanaestoro bolesnika s neovaskularnim glaukomom i simptomatskim povišenjem očnog tlaka. Kod svih bolesnika primijenjen je bevacizumab u dozi od 1,25/0,1 mL intravitrealno. Kod troje bolesnika je bevacizumab primijenjen jedan dan prije pars plana vitrektomije. Dodatna antiglaukomska terapija primijenjena je samo kod onih bolesnika u kojih očni tlak nije bio dobro kontroliran lokalnom antiglaukomskom terapijom. Najkraće razdoblje praćenja bolesnika bilo je 8 tjedana. Intravitrealna primjena bevacizumaba dovela je kod svih bolesnika do značajne regresije neovaskularizacije šarenice, što je trećega dana od primjene bevacizumaba potvrđeno biomikroskopski te fluoresceinskom angiografijom. U sedmoro bolesnika je očni tlak bio dobro kontroliran samo lokalnom antiglaukomskom terapijom. Kod četvoro bolesnika bilo je potrebno daljnje kirurško antiglaukomsko liječenje. Nisu zabilježene nuspojave kao niti komplikacije za vrijeme ili poslije intravitrealne injekcije bevacizumaba. Zaključuje se kako se intravitrealna primjena bevacizumaba čini dobrom dodatnom metodom u kombinaciji s ostalim terapijskim modalitetima liječenja neovaskularnog glaukoma. Dodatne studije kojima se ispituje dugotrajniji učinak i sigurnost intravitrealne primjene bevacizumaba su u tijeku i one će doprinijeti boljem razumijevanju farmakokinetike, toksičnosti i putova primjene bevacizumaba u liječenju neovaskularnog glaukoma

Chemical Characterization, Antioxidant Activity, and Cytotoxity of Wild-Growing and In Vitro Cultivated Rindera umbellata (Waldst. and Kit.) Bunge

The aim of this study was to comparatively analyze chemical composition and biological activity of wild- and in vitro grown Rindera umbellata. Explants were cultivated on 0.003–0.3 M sucrose, fructose, or glucose. HPLC-DAD for quantifying rosmarinic (RA) and lithospermic B (LAB) acids and GC-MS/FID for qualitative pyrrolizidine alkaloids (PAs) detection were used. Antioxidant activity (DPPH and ABTS assays) and cytotoxicity (MTT test) were monitored. Identified PAs were 7-angeloyl heliotridane, lindelofine, 7-angeloyl heliotridine, 7-angeloyl-9-(+)-trachelanthylheliotridine, punctanecine, and heliosupine, with higher variability reported in wild-growing samples. Total phenolic contents (TPCs) were comparable in wild-growing and in vitro samples, but total flavonoid (TFC) and RA levels were multifold higher in in vitro samples. Notably, high concentration of LAB was detected in wild-growing roots. Amounts of 0.3 M and 0.1 M of sucrose were optimal for TFC and RA production, while maximal antioxidant activity was monitored in plants grown on 0.3 M sucrose. The MTT test indicated colorectal HT-29 as more sensitive than A549 lung adenocarcinoma and normal MRC-5 cells, showing selective sensitivity to wild-growing and 0.3 M sucrose samples. In conclusion, PAs in vitro, as well as TPC, TFC, RA, and LAB in both growing conditions were detected for the first time in R. umbellata

International conference Kosta P. Manojlović and the Idea of Slavic and Balkan Cultural Unification (1918-1941)

This conference is organised within the project Serbian musical identities within local and global frameworks: traditions, changes, challenges (No. 177004) financed by the Serbian Ministry of Education, Science and Technological Development. It is supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia as well as the Department of Fine Arts and Music SASA

Modelovanje odnosa doza‐odgovor između nivoa Pb i Cd u tkivima karcinoma testisa i prostate i nivoa testosterona

Exposure to lead (Pb) and cadmium (Cd) poses risk to human health. It is known that these metals have toxic impact on male reproductive system, leading to physiological disorders and infertility. Limited data indicates the carcinogenic potential of Pb, while Cd has been recognized as carcinogen. Benchmark dose-effect modeling can be applied to data from human, animal and ecotoxicological studies with the aim to obtain Benchmark dose (BMD), starting point in human health risk assessment. The aim of this study was to use Benchmark modeling to determine the dose-effect relationship between Pb and Cd levels in tissues of testicular and prostate cancer patients and serum testosterone levels. The study was conducted at the Clinical Center of Serbia, and included 104 patients with prostate and testicular cancer. Cd and Pb levels (ng/g) were measured by atomic absorption spectrophotometry in isolated healthy surrounding and carcinoma-affected tissues. Testosterone levels (ng/mL) were measured using the chemiluminescence immunoassay (CLIA) method. Dose-effect modeling was performed using PROAST70.1 software with continuous data. The dose dependence was obtained between Cd levels in tumor tissue and testosterone levels (BMD: 0.0024 ng Cd/g, prostate cancer and 0.0038 ng Cd/g, testicular cancer). Dose dependence was obtained between Cd levels in healthy surrounding tissue and testosterone levels (BMD: 0.0092 ng Cd/g). No dose dependence was obtained when modeling Pb levels as a dose. The data from this study indicate a potential link between Cd and testosterone levels, i.e. potential association of Cd exposure with male reproductive health.Izloženost olovu (Pb) i kadmijumu (Cd) predstavlja rizik po zdravlje ljudi. Poznato je da ovi metali deluju toksično na muški reproduktivni sistem dovodeći do poremećaja fizioloških funkcija i neplodnosti. Ograničen broj studija ukazuje na kancerogeni potencijal Pb, dok je Cd poznatihumani karcinogen. Benchmark modelovanje odnosa doza-efekat se teoretski može primeniti na podacima iz humanih, animalnih i ekotoksikoloških studija, sa ciljem dobijanja Benchmark doze (BMD) kao polazne tačke u proceni rizika po zdravlje ljudi. Cilj ovog rada bio je da se Benchmark modelovanjem utvrdi odnos doza-efekat između nivoa Pb i Cd u tkivima karcinoma testisa i prostate pacijenata i nivoa testosterona u serumu. Studija je sprovedena na Kliničkom centru Srbije i uključila je 104 pacijenta sa karcinomom prostate i testisa. Nivoi Cd i Pb (ng/g) su izmereni metodom atomske apsorpcione spektrofotometrije u izolovanim zdravim okolonim i karcinomom zahvaćenim tkivima. Nivoi testosterona (ng/mL) su mereni pomoću metode direktnog kompetitivnog hemiluminiscentnog imunoeseja (CLIA). Modelovanje odnosa doza-efekat je vršeno pomoću PROAST 70.1 softvera (RIVM, Holandija) uz upotrebu kontinuiranih podataka. Dozna zavisnost je dobijena između nivoa Cd u tumorskom tkivu i nivoa testosterona gde je BMD vrednost iznosila 0,0024 ng Cd/g (karcinom prostate) i 0,0038 ng Cd/g (karcinom testisa). Dozna zavisnost je dobijena i za modelovanje odnosa nivoa Cd u zdravom tkivu i testosterona, sa BMD vrednošću 0,0092 ng Cd/g. Pri modelovanju nivoa Pb nije dobijena dozna zavisnost. Podaci ove studije ukazuju na potencijalnu vezu između nivoa Cd u tkivu i nivoa testosterona, ukazujući na efekte izloženosti ovom metalu na muško reproduktivno zdravlje.VIII Kongres farmaceuta Srbije sa međunarodnim učešćem, 12-15.10.2022. Beogra