Phytochemical screening and in vitro antibacterial activity of Moringa oleifera (Lam.) leaf extract

The aim of the study was to investigate the phytochemical constituents and antibacterial activity of ethanolic extract of Moringa oleifera Lam. belonging to family Moringaceae. Distilled water and ethanol was used to extract the bioactive compounds from the leaves of M. oleifera to detect the phytochemical constituents and to screen its antibacterial activity. The phytochemical constituents were screened by qualitative analysis method. The phytochemical screening indicated the presence of tannins, flavonoids, glycosides, terpenoids, phenols, etc., in leaf extract of M. oleifera. The antibacterial activity of ethanolic leaf extract of M. oleifera was examined against gram positive bacteria (Staphylococcus aureus) and gram negative bacteria (Escherichia coli). Antibacterial assay were done with ethanolic extract of M. oleifera in volumes 50, 100, 150 and 200 μL/well, using agar well diffusion method. The study showed that ethanolic extract of M. oleifera showed potent antibacterial activity against S. Aureus and E. coli

Cobb-Douglas Based Firm Production Model under Fuzzy Environment and its Solution using Geometric Programming

In this paper, we consider Cobb-Douglas production function based model in a firm under fuzzy environment, and its solution technique by making use of geometric programming. A firm may use many finite inputs such as labour, capital, coal, iron etc. to produce one single output. It is well known that the primary intention of using production function is to determine maximum output for any given combination of inputs. Also, the firm may gain competitive advantages if it can buy and sell in any quantities at exogenously given prices, independent of initial production decisions. On the other hand, in reality, constraints and/or objective functions in an optimization model may not be crisp quantities. These are usually imprecise in nature and are better represented by using fuzzy sets. Again, geometric programming has many advantages over other optimization techniques. In this paper, Cobb-Douglas production function based models are solved by applying geometric programming technique under fuzzy environment. Illustrative numerical examples further demonstrates the feasibility and efficiency of proposed model under fuzzy environment. Conclusions are drawn at last

Multi-item fuzzy inventory problem with space constraint via geometric programming method

In this paper, a multi-item inventory model with space constraint is developed in both crisp and fuzzy environment. A profit maximization inventory model is proposed here to determine the optimal values of demands and order levels of a product. Selling price and unit price are assumed to be demand-dependent and holding and set-up costs sock dependent. Total profit and warehouse space are considered to be vague and imprecise. The impreciseness in the above objective and constraint goals has been expressed by fuzzy linear membership functions. The problem is then solved using modified geometric programming method. Sensitivity analysis is also presented here

Study on the effect of toxicity under highly arsenic prone zone in Nadia district of West Bengal in India

The present study was carried out on the basis of status of arsenic in soil, drinking water and plants, blood, urine and faeces of animals at arsenic prone zone. Within the ambit with the environment, the examination of animals was taken into consideration. They were screened and categorised on the degree of As toxicity. For field works animals were randomly selected from arsenic prone zone. The external manifestation indicated a complex syndrome and characteristic signs such as increased heart rate and respiratory rate, red urine, congested mucous membrane, anorexia, absence of ruminal motility, diarrhoea with blood, polyuria and unusual weight loss. The haematobiochemical changes such as low Hb level, decreased level of TEC, TLC and increased level ALT, AST, BUN and creatinine. Increased level of arsenic in urine, blood and faeces than the value of control animals could be the confirmatory indication of arsenic toxicity

Prevalence and predictors of metabolic syndrome with comparison of myoinositol and metformin in PCOS women

Background: The objective is to determine the prevalence of metabolic syndrome (MBS) and the effects of insulin sensitizers to improve the clinical and hormonal milieu for better reproductive outcome in PCOS women.Methods: This prospective cross-sectional study was conducted on 50 PCOS women and 50 age matched control to determine the prevalence of the MBS in two tertiary hospitals over one year. Diagnosis of PCOS was based on at least two of ESHRE/ASRM criteria and diagnosis of MBS was based on at least three of NCEPATPIII criteria. Patients already diagnosed as PCOS were treated with insulin sensitizers myoionositol and metformin which were compared.Results: The study revealed that the prevalence of MBS was 40 % (20/50) nearly 4-fold higher than that of control groups. Among PCOS women, the most prevalent MBS factors were high BMI (52%) and low serum HDL-C (42%). The least prevalent factor was high fasting serum glucose level (16%). The resumption of spontaneous regular menstrual cycle and pregnancy rate in infertile groups of PCOS patients with myoionositol and metformin were 61% vs. 26% and 50% vs.91% respectively. The myoionositol group did not require any extra ovulating agents for pregnancy, while 7 out of 11 patients in metfromin group needed clomiphen citrate for ovulation induction to achieve pregnancy.  With myoinositol there is significant reduction of weight, BMI, LH/FSH ratio and fasting insulin level; whereas metformin shows decrement of weight and BMI only.Conclusions: The prevalence of MBS in PCOS is nearly 4 times in present study and there is significant improvement of symptom profile, weight, BMI and change of hormonal pattern in myoinositol group

Effects of clomiphene citrate on seminal parameters in idiopathic oligospermia: a single blinded prospective randomized controlled trial

Background: About 15% married couples face problem of infertility of which male factor is implicated in 20% of them. In about 30% infertile men no causative factor is found and the condition is termed “Idiopathic”. They are often treated by empirical medications or Assisted Reproductive Technologies (ART) although success in few; our present study is carried on effects of clomephene citrate on seminal parameters in idiopathic oligospermia.Methods: This single blinded prospective randomized controlled trial was conducted at the infertility clinic with 200 idiopathic oligospermic men (sperm count 2 years is more in group C than group p (4.3±3.06 versus 4.1±2.57). The semen volume, spermatozoa count, sperm motility and not normal form are all increased after three months of treatment in group C as are biochemical parameters like total testosterone and serum FSH, LH and as also pregnancy rate (22% versus 4%).Conclusions: This study showed clomiphene citrate treatment protocol is inexpensive, relatively safe and easy to administer and improves semen volume, sperm count and sperm motility but not sperm morphology by increasing serum testosterone, FSH and LH in male and resulting in improved pregnancy rate in female partners

Bioequivalence study of two formulations containing 400 mg dexibuprofen in healthy Indian subjects

Objective: This study presents the results of two-period, two-treatment crossover investigations on 24 healthy Indian male subjects to assess the bioequivalence of two oral formulations containing 400 mg of dexibuprofen (CAS 51146-56-6). An attempt was also made to study the pharmacokinetics of dexibuprofen in the local population of Indian origin.Method: Both of the formulations were administered orally as a single dose separated by a one-week washout period. The concentration of dexibuprofen in plasma was determined by a validated HPLC method with UV detection using carbamazepine as internal standard. The formulations were compared using the parameters area under the plasma concentration-time curve (AUC0-t), area under the plasma concentration-time curve from zero to infinity (AUC0-∞), peak plasma concentration (Cmax), and time to reach peak plasma concentration (tmax).Results: The results of this investigation indicated that there were no statistically significant differences between the logarithmically transformed AUC0-∞ and Cmax values of the two preparations. The 90 % confidence interval for the ratio of the logarithmically transformed AUC0-t, AUC0-∞ and Cmax were within the bioequivalence limit of 0.8-1.25 and the relative bioavailability of the test formulation was 99.04 % of that of reference formulationjok?.Conclusion: Thus, these findings clearly indicate that the two formulations are bioequivalent in terms of rate and extent of drug absorption. Both preparations were well tolerated with no adverse reactions observed throughout the study