Comparison of Mechanical, Assisted and Manual Harvest of Origanum vulgare L.

In Italy, the production of Origanum vulgare L. is lower than the national market demand, so there is an increasing interest in this crop, even if the manual harvest and bunch binding represent ca. 60% of the production cost. The aim of this work is to evaluate the harvest efficiency, as well as the working capacity, productivity and quality of mechanical harvesting by means of a reaper-binder designed for cereal and forage crops and assisted harvesting by means of a long-reach edge trimmer. In fact, the final aim is to suggest a machine and a method for oregano harvesting that could significantly reduce the time and, therefore, the cost of this crop operation, while achieving a product quality similar to that obtained by manual harvesting. Tests of mechanical and assisted harvesting were carried out using a reaper-binder after modifying it (i.e., reducing its forward speed, cutting height and bunch size to improve its working capacity, productivity and quality) and an edge trimmer, respectively. The tests of mechanical and assisted harvesting were compared with manual harvesting. In the testing field, with an irregular shape and an area of one hectare, nine rows were selected and divided into three replications, each comprising three tests, i.e., mechanical, assisted and manual harvesting. The modified reaper-binder allowed us to achieve working capacity and productivity rates much higher than those obtained with manual harvesting. Moreover, its harvest quality, in terms of bunch weight and binding height, favourably compares with that manually obtained. Furthermore, the edge trimmer allowed us to achieve working capacity and productivity rates lower than those obtained by means of the reaper-binder but much higher than in manual harvesting. Thus, the reaper-binder can minimise the harvest time and, therefore, cost while harvesting bunches slightly bigger than those manually harvested, even if it requires a high initial investment cost. Instead, the edge trimmer can be a cheap solution for reducing the harvest time and cost. Therefore, mechanical and assisted harvesting could spread oregano production in areas of inland Sicily and other Mediterranean regions that are often cultivated with low-profit herbaceous plant species or lie fallow. Thus, it would be possible to increase farmers’ incomes and job opportunities, as well as preventing or minimising the hydrogeological instability in these areas

Potential production of biogas from prinkly pear (opuntia ficus-indica L.) in sicilian uncultivated areas

The aim of this work is to evaluate the potential production of biogas and, indirectly, biomethane or electric and thermal energy, from prinkly pear (Opuntia ficus-indica L.), to be grown in a part of Sicilian uncultivated areas and co-digested together with the available livestock manure and slurry. In order to increase the Renewable Energy Sources (RES) and reduce the dependency from fossil ones, the conversion of biomass into biogas through Anaerobic Digestion (AD) process is paramount for producing biomethane, to be used as fuel for means of transport and agricultural machines or heating, or electric and thermal energy through Combined Heat and Power (CHP) plants. Moreover, the digestate produced through AD process can be applied to soils as organic fertiliser in the place of chemical ones. Prinkly pear was supposed to be grown, by mechanising the harvest of cladods (modified stems), in a part of the Sicilian Used Agricultural Area that is currently uncultivated (totally 600, 000 ha ca.), identified by means of a GIS software. Thus it was possible to compute the potential production of biogas and, indirectly, biomethane or electric and thermal energy. The results show that the Sicilian potential production of biogas is 612, 115 103m3, from which 342, 784 103m3of biomethane could be extracted or 67, 038 MWh of electric energy and 70, 390 MWh MWh of thermal energy could be generated. Moreover the obtained digestate would be used as biofertiliser, within both conventional and organic farming. This work demonstrates that the production of RES, such as biogas from prinkly pear, represents a very profitable way of using the uncultivated areas: the income of the farmer would include not only that deriving from the sale of biomethane or electric and thermal energy but also the saving for replacing chemical fertilisers with digestate and the subsidy for producing biomethane as fuel for means of transport or electric and thermal energy from biogas

Sicilian potential biogas production from Citrus industry by-product

In Europe, Italy is the second nation after Spain in Citrus production and 49% ca. of national fruit production is located in Sicily. The by-product obtained from the industrial processing of Citrus fruits into juice and essential oils is called \u201cpastazzo\u201d or Citrus Waste (CW). This study is aimed at evaluating the Sicilian potential biogas and energy production from the above \u201cpastazzo\u201d and verifying the possibility of using this by-product for Anaerobic Digestion (AD) process within 30 km from processing plants. The areas cultivated with Citrus species in Sicily and their distribution in the various municipal districts were mapped and analysed by means of QGIS software, together with the main Citrus processing plants and the three existing AD plants. The statistical data about the amounts of the by-product of Citrus processing industry were evaluated, in order to compute the Sicilian potential biogas and energy production. In Sicily it is possible to obtain, through the use of \u201cpastazzo\u201d, a yearly production of 12,916,800 m3 ca. of biogas, equal to 24,250,930 kWh of electric energy and 25,463,477 kWh of thermal energy or 6,200,064 m3 of biomethane. This high potential biogas and energy production is not be currently used in a sustainable way from the energetic and economic points of view, because the existing AD plants are farer than 30 km from Citrus processing plants. The CW produced in Sicily could be used inside a bio-reactor, together with other raw materials (e.g. pomace and wastewater from olive oil mills, cereal straw, poultry manure and Italian sainfoin or Hedysarum coronarium), for AD process

EFFETTI DEL CAMPO MAGNETICO ELF SU ALCUNE SPECIE VEGETALI DI INTERESSE ORNAMENTALE ED ECOLOGICO

Diversi studi riportati nella letteratura scientifica di riferimento, effettuati sia in vivo che in vitro, hanno evidenziato effetti positivi determinati dall’esposizione dei tessuti vegetali al campo magnetico EL

Intravital imaging reveals p53-dependent cancer cell death induced by phototherapy via calcium signaling.

One challenge in biology is signal transduction monitoring in a physiological context. Intravital imaging techniques are revolutionizing our understanding of tumor and host cell behaviors in the tumor environment. However, these deep tissue imaging techniques have not yet been adopted to investigate the second messenger calcium (Ca2+). In the present study, we established conditions that allow the in vivo detection of Ca2+ signaling in three-dimensional tumor masses in mouse models. By combining intravital imaging and a skinfold chamber technique, we determined the ability of photodynamic cancer therapy to induce an increase in intracellular Ca2+ concentrations and, consequently, an increase in cell death in a p53-dependent pathway

Use of biochar as peat substitute for growing substrates of Euphorbia × lomi potted plants

Biochar from conifers wood was used in soilless culture as growing substrate alternative to peat for ornamental crops. Potted plants of Euphorbia × lomi Rauh cv. 'Ilaria' were grown with different mixtures (v:v) of brown peat and biochar in order to evaluate main physical and chemical characteristics of this biomaterial as well as its effect on plant growth, ornamental characteristics and nutrients uptake. Biochar addition to peat increased pH, EC and K content of the growing substrates, as well as air content and bulk density. Biochar content of substrates significantly affected plant growth and biomass partitioning: higher number of shoots and leaves, leaf area and leaf dry weight were recorded in plants grown in 40% peat-60% biochar, with respect to plants grown in 100% peat and secondarily in 100% biochar. Leaf chlorophyll content was higher in plants grown in 60% and 80% biochar, while biomass water use efficiency was higher with 60% biochar. Plant uptake of K and Ca increased as biochar content of the substrates increased. Hence, a growing substrate containing 40% brown peat and 60% conifers wood biochar was identified as the more suitable mixture allowing to have a high-quality production of Euphorbia × lomi potted plants

Development and subunit composition of synaptic NMDA receptors in the amygdala: NR2B Synapses in the adult central amygdala

NMDA receptors are well known to play an important role in synaptic development and plasticity. Functional NMDA receptors are heteromultimers thought to contain two NR1 subunits and two or three NR2 subunits. In central neurons, NMDA receptors at immature glutamatergic synapses contain NR2B subunits and are largely replaced by NR2A subunits with development. At mature synapses, NMDA receptors are thought to be multimers that contain either NR1/NR2A or NR1/NR2A/NR2B subunits, whereas receptors that contain only NR1/NR2B subunits are extrasynaptic. Here, we have studied the properties of NMDA receptors at glutamatergic synapses in the lateral and central amygdala. We find that NMDA receptor-mediated synaptic currents in the central amygdala in both immature and mature synapses have slow kinetics and are substantially blocked by the NR2B-selective antagonists (1S, 2S)-1-(4-hydroxyphenyl)-2-(4-hydroxy-4-phenylpiperidino)-1-propano and ifenprodil, indicating that there is no developmental change in subunit composition. In contrast, at synapses on pyramidal neurons in the lateral amygdala, whereas NMDA EPSCs at immature synapses are slow and blocked by NR2B-selective antagonists, at mature synapses their kinetics are faster and markedly less sensitive to NR2B-selective antagonists, consistent with a change from NR2B to NR2A subunits. Using real-time PCR and Western blotting, we show that in adults the ratio of levels of NR2B to NR2A subunits is greater in the central amygdala than in the lateral amygdala. These results show that the subunit composition synaptic NMDA receptors in the lateral and central amygdala undergo distinct developmental changes

Coarse Grained Density Functional Theories for Metallic Alloys: Generalized Coherent Potential Approximations and Charge Excess Functional Theory

The class of the Generalized Coherent Potential Approximations (GCPA) to the Density Functional Theory (DFT) is introduced within the Multiple Scattering Theory formalism for dealing with, ordered or disordered, metallic alloys. All GCPA theories are based on a common ansatz for the kinetic part of the Hohenberg-Kohn functional and each theory of the class is specified by an external model concerning the potential reconstruction. The GCPA density functional consists of marginally coupled local contributions, does not depend on the details of the charge density and can be exactly rewritten as a function of the appropriate charge multipole moments associated with each lattice site. A general procedure based on the integration of the 'qV' laws is described that allows for the explicit construction the same function. The coarse grained nature of the GCPA density functional implies great computational advantages and is connected with the O(N) scalability of GCPA algorithms. Moreover, it is shown that a convenient truncated series expansion of the GCPA functional leads to the Charge Excess Functional (CEF) theory [E. Bruno, L. Zingales and Y. Wang, Phys. Rev. Lett. {\bf 91}, 166401 (2003)] which here is offered in a generalized version that includes multipolar interactions. CEF and the GCPA numerical results are compared with status of art LAPW full-potential density functional calculations for 62, bcc- and fcc-based, ordered CuZn alloys, in all the range of concentrations. These extensive tests show that the discrepancies between GCPA and CEF are always within the numerical accuracy of the calculations, both for the site charges and the total energies. Furthermore, GCPA and CEF very carefully reproduce the LAPW site charges and the total energy trends.Comment: 19 pages, 11 figure