The Neanderthal Meal: A New Perspective Using Faecal Biomarkers

Neanderthal dietary reconstructions have, to date, been based on indirect evidence and may underestimate the significance of plants as a food source. While zooarchaeological and stable isotope data have conveyed an image of Neanderthals as largely carnivorous, studies on dental calculus and scattered palaeobotanical evidence suggest some degree of contribution of plants to their diet. However, both views remain plausible and there is no categorical indication of an omnivorous diet. Here we present direct evidence of Neanderthal diet using faecal biomarkers, a valuable analytical tool for identifying dietary provenance. Our gas chromatography-mass spectrometry results from El Salt (Spain), a Middle Palaeolithic site dating to ca. 50,000 yr. BP, represents the oldest positive identification of human faecal matter. We show that Neanderthals, like anatomically modern humans, have a high rate of conversion of cholesterol to coprostanol related to the presence of required bacteria in their guts. Analysis of five sediment samples from different occupation floors suggests that Neanderthals predominantly consumed meat, as indicated by high coprostanol proportions, but also had significant plant intake, as shown by the presence of 5β-stigmastanol. This study highlights the applicability of the biomarker approach in Pleistocene contexts as a provider of direct palaeodietary information and supports the opportunity for further research into cholesterol metabolism throughout human evolution.NASA Astrobiology Institute (Grant NNA13AA90A

On identifying Palaeolithic single occupation episodes: archaeostratigraphic and technological approaches to the Neanderthal lithic record of stratigraphic unit xa of El Salt (Alcoi, eastern Iberia)

Within the framework of archaeological palimpsest dissection, stratigraphic association of lithic remains with hearths and other archaeological materials in undisturbed Neanderthal contexts allows us to seek patterns in lithic and faunal assemblage composition, assess the degree of time averaging within assemblages and investigate the spatial distribution of archaeological remains. So far, the European Neanderthal record shows variability in such spatial parameters, not only among different geographic regions but also across time. This approach has been employed to draw conclusions about the main features of Neanderthal occupations from in situ archaeological contexts within individual site sequences. As contribution to this topic, we present new results from our ongoing archaeostratigraphic investigation of stratigraphic unit xa from El Salt (Alcoi, Alacant, eastern Iberia). Our previous study, based on stratigraphic analysis of the lithic record consisting of raw material units, yielded several micropalimpsests within unit xa. Here, we carry out further technological and spatial analysis of the micropalimpsest units. The results obtained from it suggest that we may be able, in specific cases, to recognise indicators of diachrony within the context of these archaeostratigraphic units. This confirms the existence of a micropalimpsest, and suggests that we have the chance to find analytical frames that are even closer to the human temporal scale than the AU. This should be the target of future interdisciplinary behavioural study of El Salt unit xa. This work illustrates the potential of a spatial and archaeostratigraphic approach to stone tool technology and brings to light the importance of archaeological palimpsest dissection as a first step for behavioural analysis in Palaeolithic research.Archaeological excavations at El Salt rockshelter are funded by the Spanish central government through the project called Neandertales en la montaña alicantina: un enfoque multianalítico (MEC-FEDER HAR2015-68321-P), and by the Valencian autonomous government through public research funding (DOGV P-0300900-H). One of the authors (AM) is also funded by Universitat d’Alacant through a university faculty formation grant (UAFPU 2018-049)

Investigating hydrogen isotope variation during heating of n-Alkanes under limited oxygen conditions: implications for palaeoclimate reconstruction in archaeological settings

This paper reports on a series of heating experiments that focus on n-alkanes extracted from leaf, bark, and xylem tissues of the Celtis australis plant. These lipid biomarkers were analysed for their compound-specific hydrogen isotopic composition (δ2Hwax) under limited oxygen conditions at 150, 250, 350, and 450 °C. Our results reveal isotopic variations in wax lipids of different plant organs during short-term low-temperature combustion. We conclude that, in the absence of a detailed characterisation of the depositional environment in advance of sampling, δ2Hwax values in archaeological or otherwise highly anthropogenic environments should be interpreted cautiously. In addition, we observed that variation in δ2Hwax of leaves is minimal at temperatures ≤ 350 °C, highlighting the potential for δ2Hwax in thermally altered combustion substrates to yield palaeoclimate information, which could allow researchers to investigate links between archaeological and climatic records at a high spatial and temporal resolution.info:eu-repo/semantics/publishedVersio

La preservación del registro arqueomagnético en estructuras de combustión experimentales con 2 y 5 años de antigüedad

Trabajo presentado en: Magiber XI, 4-7 de septiembre de 2019, Condeixa a NovaJCYL-EFRD project BU235P18 and the MINECO-EFRD projects HAR2015-68321-P and CGL2016-77560, as well as the ERC Consolidator Grant project PALEOCHAR (648871

Compound-specific carbon isotope analysis of short-chain fatty acids from pine tissues: characterizing paleo-fire residues and plant exudates

Different types of plant tissues and resin can account for the wax lipids found in sedimentary contexts and archaeological samples. Consequently, there is increasing research to characterize the fatty acid carbon isotope ratios of different plant anatomical parts and their plant exudates (resin). With the aim to explore isotopic differences between plant tissues, state of the fine organic matter, effect of thermal degradation, and to identify plant residues we measured the δ13C values of short-chain fatty acids (δ13C16:0 and δ13C18:0) in: i) dead and fresh (collected and immediately dried) pine needles and branches (Pinus canariensis) and pine resin from laboratory-controlled heating experiments and ii) sediment and charred pine tissue samples from a wild pine forest fire. Our results are compared to previously published experimental open-air fire experiments and pine-fuelled archaeological combustion features. We found that for both fatty acid types, there are differences in δ13C signatures among anatomical parts and initial moisture content. These data allow us to characterize the isotopic signature of pine tissue and the effect of degradation on isotopic biomarkers, as well as to estimate combustion temperatures in pine-fuelled anthropogenic fires.Grant project PALEOCHAR – 648871info:eu-repo/semantics/publishedVersio

Una visión geoarqueológica general del yacimiento musteriense de El Salt (Alcoy, Alicante) a partir de la micromorfología

En aquest article es ressalten algunes de les aportacions principals de la micromorfologia de sòls a l’estudi del jaciment mosterià del Salt, amb la finalitat de destacar el paper tan important que ha tingut aquesta disciplina per a resoldre i caracteritzar aspectes tan importants com els processos de formació (deposicionals i postdeposicionals) geogènics, biogènics i antròpics de jaciments arqueològics, així com la identificació i descripció de sòls d’ocupació i estructures de combustió. Paraules clau: Micromorfologia. Processos de formació. Geoarqueologia. Mosterià. Estructures de combustió.In this article, we highlight some of the main contributions of an ongoing micromorphological study of sediments from the Mousterian site of El Salt (Alcoy, Alicante, Spain) in order to emphasize the important role played by this discipline in the reconstruction of geogenic, biogenic and anthropogenic site formation processes (depositional and postdepositional), as well as in the characterization of human occupation floors and combustion structures. Keywords: Micromorphology. Formation processes. Geoarchaeology. Mousterian. Combustion structures.En el presente artículo se resaltan algunas de las principales aportaciones de la micromorfología de suelos al estudio del yacimiento musteriense de El Salt, con el fin de destacar el importante papel que juega esta disciplina para resolver y caracterizar aspectos tan importantes como los procesos de formación (deposicionales y postdeposicionales) geogénicos, biogénicos y antrópicos de yacimientos arqueológicos así como la identificación y descripción de suelos de ocupación y estructuras de combustión. Palabras clave: Micromorfología. Procesos de formación. Geoarqueología. Musteriense. Estructuras de combustion

Hydroclimate reconstruction through MIS 3 in the Middle Paleolithic site of Crvena Stijena (Montenegro) based on hydrogen-isotopic composition of sedimentary n-alkanes

This study presents a hydroclimatic reconstruction from Crvena Stijena (Montenegro, Balkan Peninsula), a rock shelter that has yielded evidence for Middle Paleolithic human occupation. The integration of lipid biomarkers, hydrogen (dD) isotopic compositions of n-alkanes, and organic elemental geochemistry in the 7-m deep vertical sedimentary sequence enables reconstruction of the main hydrological and environmental changes during the MIS 3 and their correlation with the presence at the site. We apply agglomerative hierarchical clustering and principal component analysis to the geochemical, molecular, and stable isotopic data to obtain a robust hydrological record. We find evidence of three aridity trends from the studied period, one of them correlated with the Heinrich Event 5, and humid and cold -temperate conditions in archaeology-rich layers. Our dataset also contributes to the knowledge of past hydrological variability in the Balkan Peninsula, a sensitive area to short-lived climatic shifts, and overall, in the Mediterranean region during the last glacial/interglacial cycle.(c) 2022 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).info:eu-repo/semantics/publishedVersio

An instance of Neanderthal mobility dynamics: a lithological approach to the flint assemblage from stratigraphic unit VIII of El Salt rockshelter (Alcoi, eastern Iberia)

The relationship between hunter-gatherer group mobility and lithic raw material procurement strategies is central to the study of Neanderthal productive behaviours. In this framework, determination of flint procurement sources through lithological analysis is key to infer Neanderthal group mobility patterns. El Salt rockshelter (Alcoi, Alacant, eastern Iberia) features different nearby flint sources, including primary outcrops and secondary deposits containing flint. In this study, we sourced the stratigraphic unit viii archaeological flint assemblage based on identification of geogenic and postgenetic lithological traits. Our results indicate that flint procurement at El Salt during the stratigraphic unit viii Neanderthal occupations was mainly linked to Pleistocene secondary deposits along the upper and middle courses of Serpis river. The artefacts were made predominantly on alluvially reworked nodules of different flint types. Connecting these procurement areas with their corresponding knapping products reveals a direct relationship between flint-source distance and degree of technical intervention, and defines a hypothetically unidirectional series of rivershore itineraries of procurement.This work has been accomplished during the valid period of the research project titled Clima e interacciones humanas en el Mediterráneo central ibérico durante el MIS 4 (IBEMIS4), granted by the Spanish central government (PID2019-107113RB-I00). The first author (AM) is funded by Universitat d’Alacant through a university faculty formation grant (UAFPU2018-049). The second (SSR) and the fourth (LP) are funded by the Valencian autonomous government through a predoctoral research-staff contracting grant (ACIF/2021/407) and a postdoctoral research-staff contracting grant (APOSTD/2020/202), respectively

Multidisciplinary evidence of an isolated Neanderthal occupation in Abric del Pastor (Alcoi, Iberian Peninsula)

Testing Neanderthal behavioural hypotheses requires a spatial–temporal resolution to the level of a human single occupation episode. Yet, most of the behavioural data on Neanderthals has been obtained from coarsely dated, time-averaged contexts affected by the archaeological palimpsest effect and a diversity of postdepositional processes. This implies that time-resolved Neanderthal behaviour remains largely unknown. In this study, we performed archaeostratigraphic analysis on stratigraphic units ive, ivf, ivg, va, vb and vc from Abric del Pastor (Alcoi, Iberian Peninsula). Further, we isolated the archaeological remains associated with the resulting archaeostratigraphic unit and applied raw material, technological, use-wear, archaeozoological and spatial analyses. Our results show a low-density accumulation of remains from flintknapping, flint tool-use and animal processing around a hearth. These data provide a time-resolved human dimension to previous high-resolution environmental and pyrotechnological data on the same hearth, representing the first comprehensive characterisation of a Neanderthal single occupation episode. Our integrated, multidisciplinary method also contributes to advance our understanding of archaeological record formation processes.This paper has been developed during the research project Clima e interacciones humanas en el Mediterráneo central ibérico durante el MIS 4 (IBEMIS4) funded by the Spanish Government (PID2019-107113RB-I00). This paper is part of SSR’s PhD thesis and all authors agree. Several authors developed their work within different research groups/projects/contrats: SSR by the Generalitat Valenciana through a predoctoral research-staff contracting Grant (ACIF/2021/407); AM by Universitat d’Alacant through a university faculty formation Grant (UAFPU2018-049); MB by the Spanish central government through a university faculty formation Grant (FPU17/02885); LP is beneficiary on postdoctoral research Grant (APOSTD/2020/202) funded by the Generalitat Valenciana and European Social Fund. CMH, BG and CM are part of the research group SCRP (Sociedades cazadoras recolectoras paleolíticas), MB and LP are part of the research group PREMEDOC (GIUV2015-213), CM is part of the research group MBA (Micromorfología y Biomarcadores arqueológicos), and MV is a part of the ‘Neanderthal behaviour and paleoecology in Mediterranean ecosystems’ project (PID2019-103987GB-C31)

The formation, alteration and significance of pyrogenic magnetic fabric in mid-paleolithic burnt cave facies

Trabajo presentado en: Magiber XI, 4-7 de septiembre de 2019, Condeixa a NovaB. Bradák acknowledges the financial support of project BU235P18 (Junta de Castilla y Leon, Spain) and the European Regional Development Fund (ERD)