61 research outputs found
Single-reaction, multiplex, real-time rt-PCR for the detection, quantitation, and serotyping of dengue viruses.
open15siThis research was supported by the National Institutes of Health grant 1 RC4 TW008781-01. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Background:
Dengue fever results from infection with one or more of four different serotypes of dengue virus (DENV).
Despite the widespread nature of this infection, available molecular diagnostics have significant limitations. The aim of this
study was to develop a multiplex, real-time, reverse transcriptase-PCR (rRT-PCR) for the detection, quantitation, and
serotyping of dengue viruses in a single reaction.
Methodology/Principal Findings:
An rRT-PCR assay targeting the 5
9
untranslated region and capsid gene of the DENV
genome was designed using molecular beacons to provide serotype specificity. Using reference DENV strains, the assay was
linear from 7.0 to 1.0 log
10
cDNA equivalents/
m
L for each serotype. The lower limit of detection using genomic RNA was 0.3,
13.8, 0.8, and 12.4 cDNA equivalents/
m
L for serotypes 1–4, respectively, which was 6- to 275-fold more analytically sensitive
than a widely used hemi-nested RT-PCR. Using samples from Nicaragua collected within the first five days of illness, the
multiplex rRT-PCR was positive in 100% (69/69) of specimens that were positive by the hemi-nested assay, with full serotype
agreement. Furthermore, the multiplex rRT-PCR detected DENV RNA in 97.2% (35/36) of specimens from Sri Lanka positive
for anti-DENV IgM antibodies compared to just 44.4% (16/36) by the hemi-nested RT-PCR. No amplification was observed in
80 clinical samples sent for routine quantitative hepatitis C virus testing or when genomic RNA from other flaviviruses was
tested.
Conclusions/Significance:
This single-reaction, quantitative, multiplex rRT-PCR for DENV serotyping demonstrates superior
analytical and clinical performance, as well as simpler workflow compared to the hemi-nested RT-PCR reference. In
particular, this multiplex rRT-PCR detects viral RNA and provides serotype information in specimens collected more than five
days after fever onset and from patients who had already developed anti-DENV IgM antibodies. The implementation of this
assay in dengue-endemic areas has the potential to improve both dengue diagnosis and epidemiologic surveillance.openWaggoner JJ;Abeynayake J;Sahoo MK;Gresh L;Tellez Y;Gonzalez K;Ballesteros G;Pierro AM;Gaibani P;Guo FP;Sambri V;Balmaseda A;Karunaratne K;Harris E;Pinsky BAWaggoner JJ;Abeynayake J;Sahoo MK;Gresh L;Tellez Y;Gonzalez K;Ballesteros G;Pierro AM;Gaibani P;Guo FP;Sambri V;Balmaseda A;Karunaratne K;Harris E;Pinsky B
Evaluation of an automated system for the quantitation of human Herpesvirus-6 DNA from clinical specimens
Background: Quantitation of human herpesvirus-6 (HHV-6) DNA in clinical specimens is important for the diagnosis and management of HHV-6-associated infection and reactivation in immunocompromised patients, particularly transplant recipients. Methods: The analytical performance of the Altona RealStar ASR HHV-6 qPCR on the semi-automated AltoStar AM16 system was assessed using HHV-6 reference material in plasma and cerebral spinal fluid (CSF). Qualitative and quantitative agreement was determined using 123 clinical EDTA plasma specimens tested using a laboratory-developed HHV-6 qPCR. Results: The 95% Lower Limit of Detection was 20 IU/mL [95% confidence interval (CI): 10 to 29] in plasma and 78 IU/mL (95% CI: 55 to 146) in CSF. The assay was linear from 7.0 to 2.0 log10 IU/mL in both matrices. Overall agreement of the RealStar ASR HHV-6 qPCR on the AltoStar AM16 with a laboratory-developed test was 95.9% (95% CI: 90.8 to 98.7). Passing-Bablok analysis of specimens quantifiable by both methods and at levels >1000 copies/mL revealed a regression line of Y = 1.00*X–0.20, with neither systematic (95% CI Y-intercept: −0.66 to 0.26) nor proportional (95% CI slope: 0.89 to 1.10) bias compared to the reference. Conclusions: The RealStar ASR HHV-6 qPCR on the AltoStar AM16 provides accurate quantitation for clinical monitoring of HHV-6 in immunocompromised hosts
In Situ Transformed Cobalt MetalOrganic Framework Electrocatalysts for the Electrochemical Oxygen Evolution Reaction
Deep Sequencing-Identified Kanamycin-Resistant Paenibacillus Sp. Strain Ks1 Isolated From Epiphyte Tillandsia Usneoides (Spanish Moss) In Central Florida, Usa
Paenibacillus sp. strain KS1 was isolated from an epiphyte, Tillandsia usneoides (Spanish moss), in central Florida, USA. Here, we report a draft genome sequence of this strain, which consists of a total of 398 contigs spanning 6,508,195 bp, with a G+C content of 46.5% and comprising 5,401 predicted coding sequences
Deep Sequencing-Identified Kanamycin-Resistant Paenibacillus sp. Strain KS1 Isolated from Epiphyte Tillandsia usneoides (Spanish Moss) in Central Florida, USA
Paenibacillus sp. strain KS1 was isolated from an epiphyte, Tillandsia usneoides (Spanish moss), in central Florida, USA. Here, we report a draft genome sequence of this strain, which consists of a total of 398 contigs spanning 6,508,195Â bp, with a G+C content of 46.5% and comprising 5,401 predicted coding sequences
Transcription Factor NF-κB: An Update on Intervention Strategies
The nuclear factor (NF)-κB family of transcription factors are ubiquitous and pleiotropic molecules that regulate the expression of more than 150 genes involved in a broad range of processes including inflammation, immunity, cell proliferation, differentiation, and survival. The chronic activation or dysregulation of NF-κB signaling is the central cause of pathogenesis in many disease conditions and, therefore, NF-κB is a major focus of therapeutic intervention. Because of this, understanding the relationship between NF-κB and the induction of various downstream signaling molecules is imperative. In this review, we provide an updated synopsis of the role of NF-κB in DNA repair and in various ailments including cardiovascular diseases, HIV infection, asthma, herpes simplex virus infection, chronic obstructive pulmonary disease, and cancer. Furthermore, we also discuss the specific targets for selective inhibitors and future therapeutic strategies.Fil: Rajendran, Arvind. Louisiana State University; Estados UnidosFil: Inda, MarÃa Eugenia. Consejo Nacional de Investigaciones CientÃficas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias BioquÃmicas y Farmacéuticas. Departamento de MicrobiologÃa; ArgentinaFil: Bagam, Prathyusha. Southern University and A&M College; Estados UnidosFil: Sahoo, Malaya K.. Stanford University School of Medicine; Estados UnidosFil: Osorio, Diana. Louisiana State University; Estados UnidosFil: Batra, Sanjay. Louisiana State University; Estados Unidos. Southern University and A&M College; Estados Unido
Electrocatalytic Activity of Pd<sub>20–<i>x</i></sub>Ag<sub><i>x</i></sub> Nanoparticles Embedded in Carbon Nanotubes for Methanol Oxidation in Alkaline Media
Low
Pd content and highly active bimetallic PdAg electrocatalytic nanoparticles
supported on functionalized multiwalled carbon nanotubes (CNT) have
been developed for the electrochemical methanol oxidation reaction
(MOR). The polyol synthesis procedure is used to substitute Ag into
Pd particles in a Pd/CNT matrix. The Pd<sub>20–<i>x</i></sub>Ag<sub><i>x</i></sub>/CNT samples (<i>x</i> wt % = 0, 5, 10, and 15) are characterized by XRD, XPS, Raman spectroscopy,
and microscopy methods. The electrocatalytic activities and stabilities
of Pd<sub>20–<i>x</i></sub>Ag<sub><i>x</i></sub>/CNT catalysts were examined by cyclic voltammetry (CV), chronoamperometry
(CA), and chronopotentiometry (CP) measurements for electrochemical
oxidation of methanol in alkaline media. The Pd<sub>20–<i>x</i></sub>Ag<sub><i>x</i></sub>/CNT with <i>x</i> = 10 wt % is found to be a semialloyed sample and shows
the best catalytic activity (731 mA mg<sup>–1</sup>) compared
to the Pd/CNT catalyst (408 mA mg<sup>–1</sup>) and other samples.
This semialloyed sample also shows long stability and high resistance
toward CO poisoning. The semialloyed Pd<sub>10</sub>Ag<sub>10</sub> particles with optimized metal ratio on CNT matrix would be a promising
catalyst material for direct methanol fuel cell applications
Reverse-Transcriptase PCR Detection of Leptospira: Absence of Agreement with Single-Specimen Microscopic Agglutination Testing
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Previous issue date: 2015Stanford University School of Medicine.Department of Medicine. Division of Infectious Diseases and Geographic Medicine. Stanford, California, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas, Centro de Referência Nacional para Leptospirose, Coleção de Leptospira, WHO/PAHO Centro Colaborador para Leptospirose. Rio de Janeiro, RJ, Brasil.Stanford University School of Medicine. Department of Pathology. Stanford, California, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas, Centro de Referência Nacional para Leptospirose, Coleção de Leptospira, WHO/PAHO Centro Colaborador para Leptospirose. Rio de Janeiro, RJ, Brasil.Stanford University School of Medicine. Department of Pathology. Stanford, California, USA.Stanford University School of Medicine.Department of Medicine. Division of Infectious Diseases and Geographic Medicine. Stanford, California, USA / Stanford University School of Medicine. Department of Pathology. Stanford, California, USA.Background
Reference diagnostic tests for leptospirosis include nucleic acid amplification tests, bacterial culture, and microscopic agglutination testing (MAT) of acute and convalescent serum. However, clinical laboratories often do not receive paired specimens. In the current study, we tested serum samples using a highly sensitive real-time nucleic acid amplification test for Leptospira and compared results to MAT performed on the same specimens.
Methods/Principal Findings
478 serum samples from suspected leptospirosis cases in Rio de Janeiro were tested using a real-time RT-PCR for the diagnosis of leptospirosis, malaria and dengue (the Lepto-MD assay). The Lepto-MD assay detects all species of Leptospira (saprophytic, intermediate, and pathogenic), and in the current study, we demonstrate that this assay amplifies both Leptospira RNA and DNA. Dengue virus RNA was identified in 10 patients, and no cases of malaria were detected. A total of 65 samples (13.6%) were positive for Leptospira: 35 samples (7.3%) in the Lepto-MD assay, 33 samples (6.9%) by MAT, and 3 samples tested positive by both (kappa statistic 0.02). Poor agreement between methods was consistent regardless of the titer used to define positive MAT results or the day of disease at sample collection. Leptospira nucleic acids were detected in the Lepto-MD assay as late as day 22, and cycle threshold values did not differ based on the day of disease. When Lepto-MD assay results were added to the MAT results for all patients in 2008 (n=818), the number of detected leptospirosis cases increased by 30.4%, from 102 (12.5%) to 133 (16.3%).
Conclusions/Significance
This study demonstrates a lack of agreement between nucleic acid detection of Leptospira and single-specimen MAT, which may result from the clearance of bacteremia coinciding with the appearance of agglutinating antibodies. A combined testing strategy for acute leptospirosis, including molecular and serologic testing, appears necessary to maximize case detection
Sensitive Real-Time PCR Detection of Pathogenic Leptospira spp. and a Comparison of Nucleic Acid Amplification Methods for the Diagnosis of Leptospirosis
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Previous issue date: 2014Stanford University School of Medicine. Division of Infectious Diseases and Geographic Medicine. Department of Medicine. Stanford, CA, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. WHO/PAHO Centro Colaborador para Leptospirose. Coleção de Leptospira. Centro de Referência Nacional para Leptospirose. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.Stanford University School of Medicine. Department of Pathology. Stanford, CA, USA.Stanford University School of Medicine. Department of Pathology. Stanford, CA, USA.Stanford University School of Medicine. Department of Pathology. Stanford, CA, USA.Stanford University School of Medicine. Department of Pathology. Stanford, CA, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. WHO/PAHO Centro Colaborador para Leptospirose. Coleção de Leptospira. Centro de Referência Nacional para Leptospirose. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.Stanford University School of Medicine. Division of Infectious Diseases and Geographic Medicine. Department of Medicine. Department of Pathology. Stanford, CA, USA.Background: Bacteria of the genus Leptospira, the causative agents of leptospirosis, are categorized into pathogenic and
non-pathogenic species. However, the benefit of using a clinical diagnostic that is specific for pathogenic species remains
unclear. In this study, we present the development of a real-time PCR (rtPCR) for the detection of pathogenic Leptospira (the
pathogenic rtPCR), and we perform a comparison of the pathogenic rtPCR with a published assay that detects all Leptospira
species [the undifferentiated febrile illness (UFI) assay] and a reference 16S Leptospira rtPCR, which was originally designed
to detect pathogenic species.
Methodology/Principal Findings: For the pathogenic rtPCR, a new hydrolysis probe was designed for use with primers
from the UFI assay, which targets the 16S gene. The pathogenic rtPCR detected Leptospira DNA in 37/37 cultured isolates
from 5 pathogenic and one intermediate species. Two strains of the non-pathogenic L. biflexa produced no signal. Clinical
samples from 65 patients with suspected leptospirosis were then tested using the pathogenic rtPCR and a reference
Leptospira 16S rtPCR. All 65 samples had tested positive for Leptospira using the UFI assay; 62 (95.4%) samples tested
positive using the pathogenic rtPCR (p = 0.24). Only 24 (36.9%) samples tested positive in the reference 16S rtPCR (p,0.0001
for comparison with the pathogenic rtPCR and UFI assays). Amplicon sequencing confirmed the detection of pathogenic
Leptospira species in 49/50 cases, including 3 cases that were only detected using the UFI assay.
Conclusions/Significance: The pathogenic rtPCR displayed similar sensitivity to the UFI assay when testing clinical
specimens with no difference in specificity. Both assays proved significantly more sensitive than a real-time molecular test
used for comparison. Future studies are needed to investigate the clinical and epidemiologic significance of more sensitive
Leptospira detection using these tests
hrHPV prevalence and type distribution in rural Zimbabwe: A community-based self-collection study using near-point-of-care GeneXpert HPV testing
ObjectivesHigh-risk human papilloma viruses (hrHPV) are the causative agents of cervical cancer, the leading cause of cancer deaths among Zimbabwean women. The objective of this study was to describe the hrHPV types found in Zimbabwe for consideration in cervical cancer screening and vaccination efforts.Design and methodsTo determine hrHPV prevalence and type distribution in Zimbabwe we implemented a community-based cross-sectional study of self-collected cervicovaginal samples with hrHPV screening using near-point-of-care Cepheid GeneXpert HPV.ResultsThe hrHPV prevalence was 17% (112/643); 33% (41/123) vs. 14% (71/520) among HIV-1-positive and -negative participants, respectively (p=2.3E-07). Typing via Xpert HPV showed very good overall agreement (77.2%, kappa=0.698) with the Seegene Anyplex II HPV HR Detection kit. The most common types were HPV16, HPV18, HPV35, HPV52, HPV58, HPV68, HPV18, and HPV51, each of which appeared in 14-20% of infections. 37% (28/76) of women with positive cytology results (ASCUS+) had a type not included in the basic vaccine and 25% (19/76) had a type not currently in the nine-valent vaccine.ConclusionshrHPV type distribution includes less common high-risk types in rural Zimbabwe. The distribution and carcinogenicity of hrHPV type distribution should be considered during screening assay design, program development, as well as vaccine distribution and design
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